DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF3 and Spata13

DIOPT Version :9

Sequence 1:	NP_612024.4	Gene:	RhoGEF3 / 38050	FlyBaseID:	FBgn0264707	Length:	3519	Species:	Drosophila melanogaster
Sequence 2:	NP_001178615.1	Gene:	Spata13 / 305938	RGDID:	1307364	Length:	1247	Species:	Rattus norvegicus

Alignment Length:	1713	Identity:	366/1713 - (21%)
Similarity:	566/1713 - (33%)	Gaps:	608/1713 - (35%)

- Green bases have known domain annotations that are detailed below.

  Fly  1828 LTKRESGGGSSGSLRSRDSSPKYLHPQATTAYLNAAVKEAPPVYSVPTGSQAKYSP--------- 1883
            :|..:.|.|...:.....|:||......::|..|...|.:.   |.|...:|:.||
  Rat     1 MTTAQDGHGQGATATCSASNPKAPKMVTSSACQNGGCKSSS---SDPEAGEARLSPSKLVRLFSG 62

  Fly  1884 -RSRFERTTERSKT----------HALGRSRK-SQFSRVGSAGPLIAHPNTANVGVSRFRSF-DT 1935
             |.|....:||.::          :||...|| ..|.|:.|: .|....|.....||:..|. |.
  Rat    63 SRKRMNSNSERPRSVVVVGNSSTWNALASFRKMGSFKRLKSS-VLQGIQNREGAEVSKEESSGDQ 126

  Fly  1936 SKS----------QRLNSCDS------GARSDLSNDELDNEDG-----------------GLNDF 1967
            .::          :.|..|.|      ||.||.|:.| |.||.                 |....
  Rat   127 GRAAPNGTIAILGKGLGRCASLGPVGDGAGSDCSDPE-DTEDAFQRSTHRSRSIRRAYGLGRISL 190

  Fly  1968 LSAGSHKFPKIGTSSVSVSPFKMQRQRSLTPDRNDSHSSTSSLRKQRSNTPESRSLTPEERRKKG 2032
            |..|....|:.....:.|...:..|...| |.|:.|..|.|.|:.             ..:||..
  Rat   191 LDLGRQSVPEPAVCEIQVRDPEPSRAVPL-PRRSKSIDSLSVLKS-------------SFKRKSA 241

  Fly  2033 SQLS-LGGARQSSSSRSNTFEGRQRHDDKTPPNISRSSSSSSYSGGESHEPLNGTGSTTVTPSAG 2096
            |.|: |.|.||..|...::|                 .:.|...||
  Rat   242 SNLTELQGDRQVPSRTLSSF-----------------FTDSEKPGG------------------- 270

  Fly  2097 SGSTSHRRALTAKQAEQEHRIRRSRSLQLTERSPNRAHKSIVNVGALVPQQRSVGVPYQQSRVPG 2161
                            .|.|.||.|| .:..:..:|..|.:.||.....::.:   |...:..||
  Rat   271 ----------------SERRTRRWRS-PIRAKDFDRVLKLVSNVKEAAWKRET---PRSAAPSPG 315

  Fly  2162 GGVFPPTIRTSPAGAKIHVNSPAPPNSSVRVRHNETDKARSFDFDYNNFSRGGGAKGSSRTAHTS 2226
            .|:.......:|. :|:|.:.....:|||     |.|            :|.||...|...:...
  Rat   316 SGLGDANPLLAPR-SKLHDDYSRRTSSSV-----EPD------------ARRGGPLSSPCASAPC 362

  Fly  2227 GSGGDSGSNQNLRVER-----ESRSFDDEICEAVLNNNNGSNGSVSGLRFLQPATDSGQVSMAST 2286
            .:...:....:|.|:.     ||:|                         .||....|    ..|
  Rat   363 TAAPYTAPEPHLDVDTAVFPLESKS-------------------------AQPLGTDG----PHT 398

  Fly  2287 SSSRLRKSNSPVSASGMEASRSPQSSGSSTNNLHQQARQSGSPQSYGTRLCDHELTYEMLRKSPI 2351
            |||    |..|....|:..:.|...:||                       .|..       .|:
  Rat   399 SSS----SPIPTDTEGLSQASSEAHTGS-----------------------QHPF-------DPV 429

  Fly  2352 MNFRRGDSGDYELPVMLRNRETINSGGNSELNFMSNETRIYEHPTTVLKSQRSLRQSPGSRDDLN 2416
                       :||..||                         |||. |.|..  |||||
  Rat   430 -----------QLPTPLR-------------------------PTTP-KPQSP--QSPGS----- 450

  Fly  2417 LEVAVGVGGDYIYRQPANQPRSSSVKAEFLTTATTKSCTKTLDTCDYWPRCAACQHKSVSGELKK 2481
                            ...|.|.||              .:|.:.|...|.....|:...
  Rat   451 ----------------TKCPSSLSV--------------LSLSSADSEERSEDAVHRQAG----- 480

  Fly  2482 GPSASGKPLTVLDNVQPHVTSVIVNEDNAVPGPQLRSLEKSNPSWTFLCSGIASICQPVQQ---- 2542
                   |:::.|.| |.....:..|::....|       |||      .|..|   |.::
  Rat   481 -------PMSLQDAV-PAAAGDVGGENSESSFP-------SNP------DGATS---PEERKEEV 521

  Fly  2543 ---TETD---HSTAQEAAEEDIVIRKVKPNKSRRTGRSRSLPSVMLRRLYLAGMPKPGSNEMGTT 2601
               ||.|   .|..:|...|...|.:.|....||||                  |:|.| :.|..
  Rat   522 RVVTEDDWRQGSALEEEQNEAPRISRRKWGSGRRTG------------------PRPLS-DYGQL 567

  Fly  2602 SDCAASLSQQIKAVSSPTLSEPCLEEQPQKGTESTDVANTGSSAGSGGLGVLQKFKRTLNNFNNK 2666
            :..:.|:.:...|...|             |.:..|
  Rat   568 ASRSLSIPEDAIATDPP-------------GEDRVD----------------------------- 590

  Fly  2667 NQLHISPLSPAAVGNNAPKTKTSPTTTTASIAPMITTTTASDPGSEAGDTSSGKYRFGPLIWRSS 2731
             ::|     ||:|                       |||:.||     .||||..|.|
  Rat   591 -RMH-----PASV-----------------------TTTSQDP-----CTSSGCPRGG------- 614

  Fly  2732 KERRKTKYNRRDKCNSGDSGIQ----------------------IELEQDEQYSRATMAVSRQDE 2774
                     ||.:..|...|:.                      :...|...|.    |||.:..
  Rat   615 ---------RRRRPISVIGGVSFYGNTQAEDVENLLVQPAARPPVPAHQVPPYK----AVSARLR 666

  Fly  2775 PRAFALTNGSCGVALSKMRAIRRTNSAKASSILGPFIVKTKIAKHLNIGESDKAERKAPESLPTR 2839
            |..|   :.|..:.|.::...|:..::..||..|   .::......|..|.|.:..:..::.| |
  Rat   667 PFTF---SQSTPIGLDRVGRRRQMKTSNVSSDGG---AESSALVDDNGSEEDFSYEELCQANP-R 724

  Fly  2840 SLSQPNGLESYGMGRPDLEDSDSDSVASNEEAISFYPTTYAEVLYNFTAGGPQELGLERGMLIEI 2904
            .| ||.|                :.:|.| |.||......||.|::......||||.:.|.:|::
  Rat   725 YL-QPGG----------------EQLAIN-ELISDGSVVCAEALWDHVTMDDQELGFKAGDVIQV 771

  Fly  2905 LRKEVGPWWFGRIKKEETNLVEDILGPELGWFPKEFVRIIHCPETDNFFIAHRAAADEAAAEEAT 2969
            |......||:||.:.:|            .|||..|||:             |...:|.....::
  Rat   772 LEASNKDWWWGRNEDKE------------AWFPASFVRL-------------RVNQEELPEPCSS 811

  Fly  2970 ARAEEGSVPVPVAAFAEDTDVTVTTDQSNVTLIVIESPPTPSAPSVDFVAQPDHSTIIRRSAVRE 3034
            ::.||           :|.|.:....:                       .|:....:|.:.::|
  Rat   812 SQGEE-----------QDEDASKARHK-----------------------HPESQQQMRTNVIQE 842

  Fly  3035 LLDTEVNYVKLLAAICDGYLPAMSKRIDIFSPNSIRLIFSNITAIYKFQRKFLEALRRGIEQ--- 3096
            :::||..|:|.|..||:||:....|...:|:...:..||.||..|||||||||:.|.:...:
  Rat   843 IMNTERVYIKHLKDICEGYIRQCRKHTGMFTVGQLATIFGNIEDIYKFQRKFLKDLEKQYNKEEP 907

  Fly  3097 --NQVAKVFLKMHKGFLCYSTYCNAYPRALIELETYDRVKDARTILENCRESQNLAELPLSAHLL 3159
              :::...||:..:||..||.|||.:|.|.:||....:....|...|.||..|.:.::.|...||
  Rat   908 HLSEIGSCFLQHQEGFAIYSEYCNNHPGACVELSNLMKQSKYRHFFEACRLLQQMIDIALDGFLL 972

  Fly  3160 APVQRICRYPLHLNEIIKSALEKGAKEGNDTDGAKSAGKTTDYEQLDVTELDIPDSQDTVNLALE 3224
            .|||:||:|||.|.|::|...:                :.:||              :.:..|.|
  Rat   973 TPVQKICKYPLQLAELLKYTTQ----------------EHSDY--------------NNIKAAYE 1007

  Fly  3225 AMRGITEAVNEGKRHSET---IARHQASFQNFKGPPLHLHSARFFLQVDATRQKQNLWNSSYTLF 3286
            ||:.:...:||.||..|:   |||.|.|...::|..:...|:......:.|:..:.........|
  Rat  1008 AMKNVACLINERKRKLESIDKIARWQVSIVGWEGLDILDRSSELIHSGELTKITRQGKTQQRVFF 1072

  Fly  3287 LFDNQLVYCKRDIIKRSHFIYKGRIFLDRCRVVNVRDG--KMFGHTIKNSLRIYSESRDKWYDFS 3349
            |||:|||.||:|:::|....||||:.:|...:|:|.||  |.:..:::|:.::.|::.|:.:.|.
  Rat  1073 LFDHQLVSCKKDLLRRDMLYYKGRMDMDEVDIVDVEDGRDKDWNLSMRNAFKLVSKATDEVHLFC 1137

  Fly  3350 FRSANRKHRFLSTLALERQFGGKALYVSEMTGFEYNYEERPGDYSDQSDYDAQDFELATGVTSGE 3414
            .|....|.|:|...|.||    :.:...:..|.|.:..::     ..:..:||  :...|.:.|.
  Rat  1138 ARKQEDKARWLQAYADER----RRVQEDQQMGMEISENQK-----KLAMLNAQ--KAGHGKSKGY 1191

  Fly  3415 SSLPESPAKSTSRLCETLPKKSQ 3437
            :|.|.:|..      ::||...|
  Rat  1192 NSCPVAPPH------QSLPPLHQ 1208

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF3	NP_612024.4	DUF4802	<754..>790	CDD:292678
		SH3	2876..2942	CDD:214620	19/65 (29%)
		RhoGEF	3031..3235	CDD:279015	64/208 (31%)
		PH_Collybistin_ASEF	3240..3373	CDD:269931	41/137 (30%)
		PH	<3285..3363	CDD:278594	29/79 (37%)
Spata13	NP_001178615.1	SH3_ASEF	729..800	CDD:212906	27/112 (24%)
		RhoGEF	839..1018	CDD:279015	64/208 (31%)
		PH_Collybistin_ASEF	1023..1161	CDD:269931	41/141 (29%)
		PH	1051..1150	CDD:278594	30/98 (31%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	116	1.000	Domainoid score	I5808
eggNOG	1	0.900	-	-		E1_KOG3519
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0001415
OrthoInspector	1	1.000	-	-		otm45073
orthoMCL	1	0.900	-	-		OOG6_103254
Panther	1	1.100	-	-	LDO	PTHR45834
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X1124
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	9	8.810