Protein Alignment RhoGEF3 and DNMBP

DIOPT Version :10

• Sequence 1: NP_612024.4
• Gene: RhoGEF3 / 38050
• FlyBaseID: FBgn0264707
• Length: 3519
• Species: Drosophila melanogaster
• Sequence 2: NP_056036.1
• Gene: DNMBP / 23268
• HGNCID: 30373
• Length: 1577
• Species: Homo sapiens
• Alignment Length: 1226
• Identity: 235/1226 - (19%)
• Similarity: 401/1226 - (32%)
• Gaps: 403/1226 - (32%)

  Fly  2329 PQSYGTRLCDHELTYEMLRKSPIMNFRRGDSGDYELPVMLRNRETINSG----------GNSELN 2383
            |.:||..|    ..::.|..:.:         |:|:...:|...|:..|          |.....
Human   244 PGTYGVAL----YRFQALEPNEL---------DFEVGDKIRILATLEDGWLEGSLKGRTGIFPYR 295

  Fly  2384 F--MSNETRIYEHPTTVLKSQRSLRQSPGSRDDLNLEVAVGVGGDYIYRQPANQPRSSSVKAEFL 2446
            |  :..:||:.|  |..|..:.||.:.|.:..|. ||..:||        ...:..:|..:||..
Human   296 FVKLCPDTRVEE--TMALPQEGSLARIPETSLDC-LENTLGV--------EEQRHETSDHEAEEP 349

  Fly  2447 TTATTKSCTKTLD--TCDYWPRCAACQHKSVSGELKKGPSASGKPLTVLDNVQPHVTSVIVNEDN 2509
            ....:::.|..|.  |.:|.....:.|.:..:|...:.|....:.....|:.....|.|:    |
Human   350 DCIISEAPTSPLGHLTSEYDTDRNSYQDEDTAGGPPRSPGVEWEMPLATDSPTSDPTEVV----N 410

  Fly  2510 AVPGPQLRSLEKSNPSWTFLCSGIASICQPVQQTETDHST---AQEAAEEDIVIRKVKPNKSRRT 2571
            .:         .|.|...|         .|..|....:||   :...:|:       .|:.....
Human   411 GI---------SSQPQVPF---------HPNLQKSQYYSTVGGSHPHSEQ-------YPDLLPLE 450

  Fly  2572 GRSRSLPSVMLRRLYLAGMPKPGSNEMGTTSDCAASLSQQIKAVSSPTLSEPCLEEQPQKGTEST 2636
            .|:|...|:..:|:|                       .|:|.:..|.|  |...
Human   451 ARTRDYASLPPKRMY-----------------------SQLKTLQKPVL--PLYR---------- 480

  Fly  2637 DVANTGSSAGSGGLGVLQKFKRTLNNFNNKNQLHISPLSPAAVGNNAPKTKTSPTTTTASIAPMI 2701
                 |||..:         .|.:....:..|||          |.|..||...|::..||:..:
Human   481 -----GSSVSA---------SRVVKPRQSSPQLH----------NLASYTKKHHTSSVYSISERL 521

  Fly  2702 TTTTASDPGSEAGDTSSGKYRFGPLIWRSSKERRKTKYNRRDKCNSGDSGIQIELEQDEQYSRAT 2766
                ...||.:|..          |:..::..            :.||....::.:..:|.
Human   522 ----EMKPGPQAQG----------LVMEAATH------------SQGDGSTDLDSKLTQQL---- 556

  Fly  2767 MAVSRQDEPRAFALTNGSCGVALSKMRAIRRTNSAKASSILGPFIVKTKIAKHLNI----GESD- 2826
                                :...|             |:.||.....||.:|.:|    .|.|
Human   557 --------------------IEFEK-------------SLAGPGTEPDKILRHFSIMDFNSEKDI 588

  Fly  2827 -----------------KAERKAPESLPTRSLSQPNGLESYGMG-------RPDLEDSDSDSVAS 2867
                             ||.|..|....|...:.|:.|....:.       ||........|...
Human   589 VRGSSKLITEQELPERRKALRPPPPRPCTPVSTSPHLLVDQNLKPAPPLVVRPSRPAPLPPSAQQ 653

  Fly  2868 NEEAISFYPTTYAEVLYNFTAGGPQELGLERGMLIEILRKEVGPWWFGRIKKEETNLVEDILGPE 2932
            ...|:|                 |:.|...| ...|.|.|| ||...|| ..::|:..
Human   654 RTNAVS-----------------PKLLSRHR-PTCETLEKE-GPGHMGR-SLDQTSPC------- 691

  Fly  2933 LGWFPKEFVRIIHCPETDNFFIAHRAAADE-----AAAEEATARAE--------EGSVPVPVAAF 2984
                |...|||   .|.:.....:..|.:|     ...::.::|||        |.::.......
Human   692 ----PLVLVRI---EEMERDLDMYSRAQEELNLMLEEKQDESSRAETLEDLKFCESNIESLNMEL 749

  Fly  2985 AEDTDVTVTTDQSNVTLIVIESPPTPSAPSVDFVAQ-PDHSTIIRRS-AVRELLDTEVNYVKLLA 3047
            .:..::|:.:.||: :|:         |||....|: |:...:.:|: .:.|||.||.:|::.|.
Human   750 QQLREMTLLSSQSS-SLV---------APSGSVSAENPEQRMLEKRAKVIEELLQTERDYIRDLE 804

  Fly  3048 AICDGYL-PAMSKRIDIFSPN-SIRLIFSNITAIYKFQRKFLEALRRGIEQNQVAKVFLKMHKGF 3110
            ...:..: |....::    || ....:|.|:..:.|..::.|.||.   ..:.|..|||. |:..
Human   805 MCIERIMVPMQQAQV----PNIDFEGLFGNMQMVIKVSKQLLAALE---ISDAVGPVFLG-HRDE 861

  Fly  3111 L--CYSTYCNAYPRALIELETYDRVKDARTILENCRESQNLAELP-------------LSAHLLA 3160
            |  .|..||..:..|:..||.|::.:..:..|::     :||:|.             |.:.|:.
Human   862 LEGTYKIYCQNHDEAIALLEIYEKDEKIQKHLQD-----SLADLKSLYNEWGCTNYINLGSFLIK 921

  Fly  3161 PVQRICRYPLHLNEIIKSALEKGAKEGNDTDGAKSAGKTTDYEQLDVTELDIPDSQDTVNLALEA 3225
            ||||:.||||.|.|::.|..|..                             ||.....|..| |
Human   922 PVQRVMRYPLLLMELLNSTPESH-----------------------------PDKVPLTNAVL-A 956

  Fly  3226 MRGITEAVNEGKRHSETIARHQASFQNFKGPPLHLHSARFFLQVDATRQKQNLWNSSYT-LFLFD 3289
            ::.|...:||.||..:.:.:::      ||....|......|.:.:..:|.|..:|... |..|.
Human   957 VKEINVNINEYKRRKDLVLKYR------KGDEDSLMEKISKLNIHSIIKKSNRVSSHLKHLTGFA 1015

  Fly  3290 NQLVYCKRDIIKRSHFIYKGRIFLDRCRVVN--VRDGKMFGHTIKNSLRIYSESRDKWYDFSFRS 3352
            .|:   |.::.:.:.     :.|..:.|::.  :||..::...|:.|..:...:....:|.....
Human  1016 PQI---KDEVFEETE-----KNFRMQERLIKSFIRDLSLYLQHIRESACVKVVAAVSMWDVCMER 1072

  Fly  3353 ANRK-------HRFLS----TLALERQFGGKALYVSEM-------TG--------FE-----YNY 3386
            .:|.       ||::|    |...||.   :.|.:|.:       ||        |:     ||.
Human  1073 GHRDLEQFERVHRYISDQLFTNFKERT---ERLVISPLNQLLSMFTGPHKLVQKRFDKLLDFYNC 1134

  Fly  3387 EERPGDYSDQSDYDAQDFELATGVTSGESSLPESPAKSTSRLCETLPKKSQSRDGI 3442
            .||.....|:...:    ||.:...:.|:        ..::|.:.|||..|...|:
Human  1135 TERAEKLKDKKTLE----ELQSARNNYEA--------LNAQLLDELPKFHQYAQGL 1178

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF3	NP_612024.4	SH3	2876..2942	CDD:214620	13/65 (20%)
		RhoGEF	3028..3234	CDD:238091	54/223 (24%)
		PH_Collybistin_ASEF	3240..3373	CDD:269931	25/146 (17%)
DNMBP	NP_056036.1	SH3_DNMBP_N1	6..56	CDD:212728
		SH3_DNMBP_N2	70..123	CDD:212729
		SH3_DNMBP_N3	149..199	CDD:212730
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	211..244		235/1226 (19%)
		SH3_DNMBP_N4	247..296	CDD:212731	10/61 (16%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	335..395		10/59 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	591..624		5/32 (16%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	639..659		4/19 (21%)
		RhoGEF	785..965	CDD:238091	54/222 (24%)
		BAR_DNMBP	1017..1208	CDD:153273	35/185 (19%)
		SH3_DNMBP_C1	1289..1345	CDD:212732
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1348..1487
		SH3_DNMBP_C2	1517..1573	CDD:213017