Protein Alignment RhoGEF3 and Spata13

DIOPT Version :10

• Sequence 1: NP_612024.4
• Gene: RhoGEF3 / 38050
• FlyBaseID: FBgn0264707
• Length: 3519
• Species: Drosophila melanogaster
• Sequence 2: XP_036014454.1
• Gene: Spata13 / 219140
• MGIID: 104838
• Length: 1295
• Species: Mus musculus
• Alignment Length: 1727
• Identity: 371/1727 - (21%)
• Similarity: 584/1727 - (33%)
• Gaps: 564/1727 - (32%)

  Fly  1794 DGNLSDRLLNLAVLAGQR--ERSPNFSTLTGN-----SPNYLTKRESGGGSSGSLRSRDSSPKYL 1851
            |.:...|..:.|.:.|..  |:.|..:.:.|.     .|..:|..:.|.|...:.....|:||..
Mouse    11 DPDAHSRCESQAAVLGDEGLEQGPPVTGVNGGLSQAAHPKNMTTAQDGHGQGATATCSASNPKAP 75

  Fly  1852 HPQATTAYLNAAVKEAPPVYSVPTGSQAKYSPRSRFERTTERSKTHALGRSRKSQFSRVGSAGPL 1916
            ....::|..|...|.:|.  |.|...:.:.|| |:..|....|      |.|.:..|....:..|
Mouse    76 KMVTSSACQNGGCKSSPS--SDPEAGETRLSP-SKLVRLFSGS------RKRMNSNSERPRSVVL 131

  Fly  1917 IAHPNTANVGVSRFR---SFDTSKSQRLNSCDSGARSDLSNDELDNEDGGLNDFLSAGSHKFPKI 1978
            :.:.:|.| .::.||   ||...||..|....:...:::|.:|...:.|           :....
Mouse   132 VGNSSTWN-ALASFRKMGSFKRLKSSVLQGIQNREGAEVSKEEPTGDPG-----------RAAPN 184

  Fly  1979 GTSSVSVSPFKMQRQRSLTP-------DRNDSHSSTSSLRKQRSNTPESRSLTPEERRKKG---- 2032
            ||  |::....:.|..||.|       |.:|...:..:.  ||| |..|||:    ||..|
Mouse   185 GT--VAILGKGLGRCTSLGPVGDGAGSDCSDPEDTEDAF--QRS-THRSRSI----RRAYGLGRI 240

  Fly  2033 SQLSLG------------GARQSSSSRSNTFEGRQRHDDKTPPNIS----RSSSSSSYSGGESHE 2081
            |.|.||            ..|....||:.....|.:..|......|    :|:|:.:...|:...
Mouse   241 SLLDLGRQPVPEPAVCEIQVRDPEPSRAVPLPRRSKSIDSLSVLKSSFKRKSASNLTELQGDRQV 305

  Fly  2082 PLNGTGSTTVTPSAGSGSTSHRRALTAKQAEQEHRIRRSRSLQLTERSPNRAHKSIVNVGALVPQ 2146
            |.....|....|....||              |.|.||       .|||.|| |....|..||..
Mouse   306 PSRTLSSFFTDPEKPGGS--------------ERRTRR-------WRSPIRA-KDFDRVLRLVSN 348

  Fly  2147 QRSVG----VPYQQSRVPGGGVFPPTIRTSPAGAKIHVNSPAPPNSSVRVRHNETDKARSFDFDY 2207
            .:...    .|...:..||.|:.......:|. :|:|.:.....:|||     |.|
Mouse   349 VKDAAWKREAPRSAAPGPGPGLGDENPLLAPR-SKLHDDYSRRTSSSV-----EPD--------- 398

  Fly  2208 NNFSRGGGAKGSSRTAHTSGSGGDSGSNQNLRVERESRSFDDEICEAVLNNNNGSNGSVSGLRFL 2272
               :|.||:........|:.......     .::.::..|..|...|....|:|...|
Mouse   399 ---ARRGGSPCIPAPCTTAPYAAPEP-----HLDVDTAVFPLETKSAQPLGNDGPRAS------- 448

  Fly  2273 QPATDSGQVSMASTSSSRLRKSNSPVSASGMEASRSPQSSGSSTNNLHQQARQSGSPQSYGTRLC 2337
                                 |.||:..       .|:.....::..|     :||...:
Mouse   449 ---------------------SPSPIPT-------DPEGLSQGSSEAH-----TGSQHPF----- 475

  Fly  2338 DHELTYEMLRKSPIMNFRRGDSGDYELPVMLRNRETINSGGNSELNFMSNETRIYEHPTTVLKSQ 2402
                       :|:           :||..||                         |||. |.|
Mouse   476 -----------NPV-----------QLPTPLR-------------------------PTTP-KPQ 492

  Fly  2403 RSLRQSPGSRDDLNLEVAVGVGGDYIYRQPANQPRSSSVKAEFLTTATTKSCTKTLDTCDYWPRC 2467
            ..  |||||                     ...|.|.||    |:.::..|..:|.|.....|..
Mouse   493 SP--QSPGS---------------------TKCPSSLSV----LSLSSADSEERTEDASHRQPGP 530

  Fly  2468 AACQHK------SVSGELKKGPSASGKPLTVLDNVQPHVTSVIVNEDNAVPGPQLRSLEKSNPSW 2526
            .:.|..      .|.||  .|.|...:.|....:.:.....|:|.||:   .||..:||:
Mouse   531 VSLQDTVLNAAGDVGGE--SGDSYFPENLDGATHPEEKKEEVLVTEDD---WPQGSALEE----- 585

  Fly  2527 TFLCSGIASICQPVQQTETDHSTAQEAAEEDIVIRKVKPNKSRR---TGRSRSLPSVMLRRLYLA 2588
                          :|.|.                   |..|||   :||..
Mouse   586 --------------EQNEA-------------------PRISRRRWGSGRRT------------- 604

  Fly  2589 GMPKPGSNEMGTTSDCAASLSQQIKAVSSPTLSEPCLEEQPQKGTESTDVANTGSSAGSGGLGVL 2653
             .|:|.| :.|..:..:.|:.:...|...|
Mouse   605 -RPRPLS-DYGQLAGRSLSIPEDAIAADPP----------------------------------- 632

  Fly  2654 QKFKRTLNNFNNKNQLHISPLSPAAVGNNAPKTKTSPTTTTASIAPMITTTTASDPGSEAGDTSS 2718
                         ::.|:..:.||:|                       |||:.||.:.:|....
Mouse   633 -------------DEDHVDRMHPASV-----------------------TTTSQDPCAPSGSCRG 661

  Fly  2719 GKYRFGPLIWRSSKERRKTKYNRRDKCNSGDSGI------QIE----------------LEQDEQ 2761
            |                     ||.:..|...|:      |:|                ..|...
Mouse   662 G---------------------RRRRPISVIGGVSFYGNTQVEDVENLLVQPAARPPVPAHQVPP 705

  Fly  2762 YSRATMAVSRQDEPRAFALTNGSCGVALSKMRAIRRTNSAKASSILGPFIVKTKIAKHLNIGESD 2826
            |.    |||.:..|..|   :.|..:.|.::...|:..::..||..|   .::......|..|.|
Mouse   706 YK----AVSARLRPFTF---SQSTPIGLDRVGRRRQMKTSNVSSDGG---AESSALVDDNGSEED 760

  Fly  2827 KAERKAPESLPTRSLSQPNGLESYGMGRPDLEDSDSDSVASNEEAISFYPTTYAEVLYNFTAGGP 2891
            .:..:..::.| |.| ||.|                :.:|.| |.||......||.|::......
Mouse   761 FSYEELCQANP-RYL-QPGG----------------EQLAIN-ELISDGSVVCAEALWDHVTMDD 806

  Fly  2892 QELGLERGMLIEILRKEVGPWWFGRIKKEETNLVEDILGPELGWFPKEFVRIIHCPETDNFFIAH 2956
            ||||.:.|.:|::|......||:||.:.:|            .|||..|||:
Mouse   807 QELGFKAGDVIQVLEASNKDWWWGRNEDKE------------AWFPASFVRL------------- 846

  Fly  2957 RAAADEAAAEEATARAEEGSVPVPVAAFAEDTDVTVTTDQSNVTLIVIESPPTPSAPSVDFVAQP 3021
            |...:|.....:::..||           :|.|.:....:                       .|
Mouse   847 RVNQEELPENCSSSHGEE-----------QDEDTSKARHK-----------------------HP 877

  Fly  3022 DHSTIIRRSAVRELLDTEVNYVKLLAAICDGYLPAMSKRIDIFSPNSIRLIFSNITAIYKFQRKF 3086
            :....:|.:.::|:::||..|:|.|..||:||:....|...:|:...:..||.||..||||||||
Mouse   878 ESQQQMRTNVIQEIMNTERVYIKHLKDICEGYIRQCRKHTGMFTVAQLATIFGNIEDIYKFQRKF 942

  Fly  3087 LEALRRGIEQ-----NQVAKVFLKMHKGFLCYSTYCNAYPRALIELETYDRVKDARTILENCRES 3146
            |:.|.:...:     :::...||:..:||..||.|||.:|.|.:||....:....|...|.||..
Mouse   943 LKDLEKQYNKEEPHLSEIGSCFLEHQEGFAIYSEYCNNHPGACVELSNLMKHSKYRHFFEACRLL 1007

  Fly  3147 QNLAELPLSAHLLAPVQRICRYPLHLNEIIK-SALEKGAKEGNDTDGAKSAGKTTDYEQLDVTEL 3210
            |.:.::.|...||.|||:||:|||.|.|::| :..|.|                 ||
Mouse  1008 QQMIDIALDGFLLTPVQKICKYPLQLAELLKYTTQEHG-----------------DY-------- 1047

  Fly  3211 DIPDSQDTVNLALEAMRGITEAVNEGKRHSET---IARHQASFQNFKGPPLHLHSARFFLQVDAT 3272
                  :.:..|.|||:.:...:||.||..|:   |||.|.|...::|..:...|:......:.|
Mouse  1048 ------NNIKAAYEAMKNVACLINERKRKLESIDKIARWQVSIVGWEGLDILDRSSELIHSGELT 1106

  Fly  3273 RQKQNLWNSSYTLFLFDNQLVYCKRDIIKRSHFIYKGRIFLDRCRVVNVRDG--KMFGHTIKNSL 3335
            :..:...:.....||||:|||.||:|:::|....||||:.:|...:|:|.||  |.:..:::|:.
Mouse  1107 KITRQGKSQQRIFFLFDHQLVSCKKDLLRRDMLYYKGRMDMDEVELVDVEDGRDKDWSLSLRNAF 1171

  Fly  3336 RIYSESRDKWYDFSFRSANRKHRFLSTLALERQFGGKALYVSEMTGFEYNYEERPGDYSDQSDYD 3400
            ::.|::.|:.:.|..|....|.|:|...|.||    :.:...:..|.|.     |.:....:..:
Mouse  1172 KLVSKATDEVHLFCARKQEDKARWLQAYADER----RRVQEDQQMGMEI-----PENQKKLAMLN 1227

  Fly  3401 AQDFELATGVTSGESSLPESPAKSTSRLCETLPKKSQ 3437
            ||  :...|.:.|.:|.|.:|..      ::||...|
Mouse  1228 AQ--KAGHGKSKGYNSCPVAPPH------QSLPPLHQ 1256

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF3	NP_612024.4	SH3	2876..2942	CDD:214620	19/65 (29%)
		RhoGEF	3028..3234	CDD:238091	67/211 (32%)
		PH_Collybistin_ASEF	3240..3373	CDD:269931	41/137 (30%)
Spata13	XP_036014454.1	PHA03247	<251..497	CDD:223021	67/385 (17%)
		SH3_ASEF	777..848	CDD:212906	27/112 (24%)
		RhoGEF	887..1066	CDD:214619	66/209 (32%)
		PH_Collybistin_ASEF	1071..1209	CDD:269931	41/141 (29%)