DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RhoGEF3 and Tiam1

DIOPT Version :9

Sequence 1:	NP_612024.4	Gene:	RhoGEF3 / 38050	FlyBaseID:	FBgn0264707	Length:	3519	Species:	Drosophila melanogaster
Sequence 2:	NP_001139358.1	Gene:	Tiam1 / 21844	MGIID:	103306	Length:	1591	Species:	Mus musculus

Alignment Length:	1774	Identity:	375/1774 - (21%)
Similarity:	600/1774 - (33%)	Gaps:	542/1774 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly  1880 KYSPRS-RFERTTERSKTHALGRS--RKSQFSRVGSAGPLIAHPNTANVGVSRFRSFDTSKSQRL 1941
            |::.|| |....|.|::..:.|::  |.|:.|...|:.|.|.. :.|..|:..|       ||
Mouse    24 KHTSRSLRLSHKTRRTRHASSGKAIHRNSEVSTRSSSTPSIPQ-SLAENGLEPF-------SQ-- 78

  Fly  1942 NSCDSGARSDLSND-ELDNEDGGLNDFLSAGSHKFPKIGTSSVSVSPFKMQRQRSLTPDRNDSHS 2005
                .||..|..:. .:|..|.||.          |...|.|            |:||..:.|..
Mouse    79 ----EGALDDFGDPIWVDRVDMGLR----------PVSYTDS------------SVTPSVDGSIV 117

  Fly  2006 STSSLRKQRSNTPESRSLTPEERRKKGSQLSLGGARQSS-SSRSNTF-------EGRQRHDDKTP 2062
            .|::..:...::.|||....:     .:.|:.||.||.. :|...||       :.|.:..|
Mouse   118 LTAASVQSMPDSEESRLYGDD-----ATYLAEGGRRQCPYTSNGPTFMETASFKKKRSKSAD--- 174

  Fly  2063 PNISRSSSSSSYSGGESHEPLNGTGSTTVTPSAGSGSTSHRRALTAKQAEQEHRIRRSRSLQLTE 2127
              |.|..|........|.|.|  |.:..:..||                 :|.....:|.:: ||
Mouse   175 --IWREDSLEFSLSDLSQEHL--TSNEEILGSA-----------------EEKDCEEARGME-TE 217

  Fly  2128 RSP------NRAHKSIVNVGALVPQQRSVGVPYQQSRVPGGGVFPPTIRTS----------PAGA 2176
            .||      .||:    ::|.|..|:.| ||     :..||    |..|.|          |..|
Mouse   218 ASPRQLSTCQRAN----SLGDLYAQKNS-GV-----KANGG----PRNRFSSYCRNLVSDIPDLA 268

  Fly  2177 KIHVNSPA-----PPNSSVRVRHNETDKARSFDFDYNNFSRGGGAKGSSRTAHTSGSGGDSGSNQ 2236
            | |...||     ||.|                 :||..        ..|.:|....|   .:|.
Mouse   269 K-HKMPPAAAEETPPYS-----------------NYNTL--------PCRKSHCLSEG---ATNP 304

  Fly  2237 NLRVERESRSFDDEICEAVLNNNNGSNGSVSGLRFLQPATDSGQVSMASTSSSRL-RKSNSPVSA 2300
            .:.:   |:|......:...:.|.|     .|..|    .||| :..|:|.:..| |:||:..|:
Mouse   305 QISL---SKSMQGRRAKTTQDVNTG-----EGSEF----ADSG-IEGATTDTDLLSRRSNATNSS 356

  Fly  2301 SGMEASRSPQSSGSSTNNLHQQARQSGSPQSYGTRLCDHELTYEMLRKSPIMNFRRGDSGDYELP 2365
            ......|:...|.|.:::....|||.               .||        ||||      ||.
Mouse   357 YSPPTGRAFVGSDSGSSSTGDAARQG---------------VYE--------NFRR------ELE 392

  Fly  2366 VMLRNRETINSGGNSELNFMSNETRIYEHPTTVLKSQRSLRQSPGSRDDLNLEVAVGVGGDYIYR 2430
            :...|.|::...|::..:..|:.|                ..||| :.|:.|..|.|.
Mouse   393 MSTTNSESLEEAGSAHSDEQSSGT----------------LSSPG-QSDILLTAAQGT------- 433

  Fly  2431 QPANQPRSSSVKAEFLTTATTK---SCTKTLDTCDYWPRCAACQHKSVSGELKKGPSASGKPLTV 2492
              ..:..:.:|| .||.....|   |.|:. ....||.....|.......:.:.|...:..|
Mouse   434 --VRKAGALAVK-NFLVHKKNKKVESATRR-KWKHYWVSLKGCTLFFYETDGRSGIDHNSVP--- 491

  Fly  2493 LDNVQPHVTSVIVNEDNAVPGPQLRSLEKSNPSWTFLCS---GIASICQPVQQTETD------HS 2548
                 .|...|..:...|||       |.....:.|..|   |.|.:.|...|||.:      ||
Mouse   492 -----KHAVWVENSIVQAVP-------EHPKKDFVFCLSNSLGDAFLFQTTSQTELENWITAIHS 544

  Fly  2549 TAQEAA-----EEDIV------IRKVK-----PNKSRRTGRSRSLPSVMLRRLYLAGMPKPGSNE 2597
            ....|.     :||.:      |:|::     ..|.::.|..: |.||            ..|.:
Mouse   545 ACAAAVARHHHKEDTLRLLKSEIKKLEQKIDMDEKMKKMGEMQ-LSSV------------TDSKK 596

  Fly  2598 MGTTSDCAASLSQQIKAVSSPTLSEPCLEEQPQKG-----TESTDVANTGSSAGSGGLGVLQKFK 2657
            ..|..|......|.::..........|.....|.|     ......|:..:....|.||:.
Mouse   597 KKTILDQIFVWEQNLEQFQMDLFRFRCYLASLQGGELPNPKRLLAFASRPTKVAMGRLGIF---- 657

  Fly  2658 RTLNNFNNKNQLHISPLSPAAVGNNAPKTKTSPTTTTASIAPMITTTTASDPGSEAG-DTSSGKY 2721
             ::::|:        .|..|..|....:.:|...:.:||       ...|...|..| ||:|.|.
Mouse   658 -SVSSFH--------ALVAARTGEIGVRRRTQAMSRSAS-------KRRSRFSSLWGLDTTSKKK 706

  Fly  2722 RFGPLIWRSSKE-----RRKTKYNRRDKCNSGDSGIQIELEQDEQYS-RATMAVSRQDEPRAFAL 2780
            :..|.|.:...|     :|..:....|....|....::.|....|:: ...:.|.....|..|.|
Mouse   707 QGRPTINQVFGEGTDAVKRSLEGIFDDTVPDGKREKEVVLPSVHQHNPDCDIWVHEYFTPSWFCL 771

  Fly  2781 TNGSCGV--------ALSKMRAIRRTNSAKASS--ILGPFIVKTKIAKHLNIGESDKAERKAPES 2835
            .|....:        |...:..|.:|:....|:  :...|:::.::..::...|.|..|....|.
Mouse   772 PNNQPALTVVRPGDTARDTLELICKTHQLDHSAHYLRLKFLMENRVQFYIPQPEEDIYELLYKEI 836

  Fly  2836 LPTRSLSQPNGLESYGMGRPDLEDSDSDSVASN---------EEAI-SFYPTTYAEVLYNFTAGG 2890
            .....::|...:|.            ||:.|.|         |:.| ..|..:..|      .|.
Mouse   837 EICPKVTQNIHIEK------------SDAAADNYGFLLSSVDEDGIRRLYVNSVKE------TGL 883

  Fly  2891 PQELGLERG-MLIEILRKEVGPWWFGRIKKEETNLVEDILG-PELGWF------PKEFVRIIHCP 2947
            ..:.||:.| .::||..:..|..        .:::::|.|. |.||..      |:..|.::..|
Mouse   884 ASKKGLKAGDEILEINNRAAGTL--------NSSMLKDFLSQPSLGLLVRTYPEPEGGVELLENP 940

  Fly  2948 -----------ETDNFFI----AHRAAADEAAAEEATARAEEGSVPVPVAAFAEDTDVTVTTDQS 2997
                       |:...|:    .|..::::.::.|..  .|||..|        |.:.:..||.|
Mouse   941 PHRVDGPVDLGESPLAFLTSNPGHSLSSEQGSSAETA--PEEGEGP--------DLESSDETDHS 995

  Fly  2998 NVTLIVI--------ESPPTPSAPSVDFVAQPDHSTIIR-------RSAVRELLDTEVNYVKLLA 3047
            :.:...:        |..|:.|:||......|..:|..:       |..:.|||:||..|||.|.
Mouse   996 SKSTEQVAAFCRSLHEMSPSDSSPSPQDATSPQLATTRQLSDADKLRKVICELLETERTYVKDLN 1060

  Fly  3048 AICDGYLPAMSKRIDIFSPNSIRLIFSNITAIYKFQRKFLEALRRGI----------EQNQVAKV 3102
            .:.:.||..:.|. ...:.:.:.::|.|:|.:.:||.:||:.|..|:          :.:|..||
Mouse  1061 CLMERYLKPLQKE-TFLTQDELDVLFGNLTEMVEFQVEFLKTLEDGVRLVPDLEKLEKVDQFKKV 1124

  Fly  3103 FLKMHKGFL-------CYSTYCNAY---PRALIELETYDRVKDARTILENCRESQNLAELPLSAH 3157
            ...:...||       .||.:|.::   |:.|::.:| |....|....:|.|:..:..   |.::
Mouse  1125 LFSLGGSFLYYADRFKLYSAFCASHTKVPKVLVKAKT-DTAFKAFLDAQNPRQQHSST---LESY 1185

  Fly  3158 LLAPVQRICRYPLHLNEIIKSALEKGAKEGNDTDGAKSAGKTTDYEQLDVTELDIPDSQDTVNLA 3222
            |:.|:||:.:|||.|.|:.  ||                   ||.|..:...||:         |
Mouse  1186 LIKPIQRVLKYPLLLRELF--AL-------------------TDAESEEHYHLDV---------A 1220

  Fly  3223 LEAMRGITEAVNE-GKRHSE-------TIAR---HQASFQNFKGPPLHLHSARFFLQVDATRQKQ 3276
            ::.|..:...:|| .|.|.|       .||.   .:....:.....|.||::..:|...|:..|
Mouse  1221 IKTMNKVASHINEMQKIHEEFGAVFDQLIAEQTGEKKEVADLSMGDLLLHTSVIWLNPPASLGK- 1284

  Fly  3277 NLWNSSYTL--FLFDNQLVYCKRDIIKRSHFIYKGRIFLDRCRVVNVRDG-----KMFGHTIKNS 3334
              |.....|  |:|...:|           .:||              ||     |:.|   .:.
Mouse  1285 --WKKEPELAAFVFKTAVV-----------LVYK--------------DGSKQKKKLVG---SHR 1319

  Fly  3335 LRIYSESRDKWYDFSFRSANRKHRFLSTLALE----RQFGGKALYVSEMTGFEYNYEERPG---D 3392
            |.||.|    |..|.||      ..:.|.||:    .....:|..|.|:...:...|.||.   .
Mouse  1320 LSIYEE----WDPFRFR------HMIPTEALQVRALPSADAEANAVCEIVHVKSESEGRPERVFH 1374

  Fly  3393 YSDQSDYDAQDFELATGVTSGESSLPESPAKSTSRLCETLPKKSQ--------------SRDGIS 3443
            ....|....:||     :.|..|.|.:...:...: .|:||...|              :|..:|
Mouse  1375 LCCSSPESRKDF-----LKSVHSILRDKHRRQLLK-TESLPSAQQYVPFGGKRLCALKGARPAMS 1433

  Fly  3444 SAENSQIVTTTSTGSLGRR 3462
            .|      .:..:.|||||
Mouse  1434 RA------VSAPSKSLGRR 1446

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF3	NP_612024.4	DUF4802	<754..>790	CDD:292678
		SH3	2876..2942	CDD:214620	14/73 (19%)
		RhoGEF	3031..3235	CDD:279015	55/223 (25%)
		PH_Collybistin_ASEF	3240..3373	CDD:269931	31/153 (20%)
		PH	<3285..3363	CDD:278594	18/84 (21%)
Tiam1	NP_001139358.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..70		13/46 (28%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	305..380		19/87 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	393..422		8/45 (18%)
		PH1_Tiam1_2	432..558	CDD:269937	30/151 (20%)
		Tiam_CC_Ex	572..669	CDD:408184	18/122 (15%)
		RBD	766..832	CDD:128731	12/65 (18%)
		PDZ_signaling	846..925	CDD:238492	21/104 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	933..1034		21/110 (19%)
		RhoGEF	1044..1233	CDD:214619	55/223 (25%)
		PH2_Tiam1_2	1235..1406	CDD:269957	45/217 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1456..1481
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3519
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810