Protein Alignment RhoGEF3 and tiam1b

DIOPT Version :9

• Sequence 1: NP_612024.4
• Gene: RhoGEF3 / 38050
• FlyBaseID: FBgn0264707
• Length: 3519
• Species: Drosophila melanogaster
• Sequence 2: XP_009290217.1
• Gene: tiam1b / 100330358
• ZFINID: ZDB-GENE-120221-1
• Length: 1562
• Species: Danio rerio
• Alignment Length: 1651
• Identity: 318/1651 - (19%)
• Similarity: 542/1651 - (32%)
• Gaps: 473/1651 - (28%)

  Fly  2037 LGGARQSSSSRSNTFEGRQRHDDKTPPNISRSSSSSSYSGGESHEPLNGTGSTTVTPSAGSGSTS 2101
            :|.|...|.......:|:::...|......|.||..:|             .|..||   ||...
Zfish     1 MGNAESHSGGHGYYGDGQRQLSRKHMSRSLRLSSKQTY-------------CTRRTP---SGKPE 49

  Fly  2102 HRRALTAKQAEQEHRIRRSRSLQLTERSPNRAHKSIVNVGALVPQQRSVGVPYQQSRVPGGGVFP 2166
            ||.:.|:.::.....|.:|.:....|.         .|...|:|...|   |....|| ...:.|
Zfish    50 HRNSETSTRSSSTPSIPQSLADHGLEP---------FNDSDLLPDFSS---PIWVDRV-AMNLRP 101

  Fly  2167 PTIRTSPAGAKIHVNSPA--PPNSSVRVRHNETDKARSFDFDYNNFSRGGGAKGSSRTAHTSGSG 2229
            .:....|.      :|||  ||..:.:|:|..:...|       :.|:.|..|..|::|
Zfish   102 MSFHNQPP------HSPAVKPPEENAQVQHTRSPTTR-------DASQDGFKKKRSKSA------ 147

  Fly  2230 GDSGSNQNLRV-----ERESRSFDDEICEA--------------VLNNNNGSNGSVSGLR----- 2270
             |.....:|.|     .:|..:..:|:.:.              .|.....|:|..:...
Zfish   148 -DMWREDSLEVSLSDLSQEHMTSTEEMADTPDTHSAQDRANSLDELYGTKPSHGRYARWHKGGAG 211

  Fly  2271 -----FLQPATDSGQVSMASTSSSRLRK--SNSPVSASGMEASRSPQSSGSSTNNLHQQARQSGS 2328
                 .:.|..|.|....|.|...|...  |..|.:..|:...|..|:....|.........||.
Zfish   212 DEEKMMMSPCEDDGAAYGAFTLPCRRSHCLSEGPTNQQGVMQGRRAQTIQDVTAADGSDYEDSGI 276

  Fly  2329 PQSYGTRLCDHELTYEMLRKSPIMN-------------FRRGDSGDYELPVMLRNRETINSGGNS 2380
            .   |..:.|.||:....|:...|:             |...|||              :|.|.:
Zfish   277 D---GVTVADSELSQHQSRRYKTMSVSFSAYSTPAGGAFTGSDSG--------------SSSGAA 324

  Fly  2381 ELNFMSNETRIYEHPTTVLKSQRSLRQSPGSRDDLNLEVAVGVGGDYIYRQPANQPRSSSVKAEF 2445
            ..| ..|...:||:                .|.:|...|.           ||..|...|
Zfish   325 ATN-TDNTQGVYEN----------------FRQELEQSVC-----------PAEAPEDRS----- 356

  Fly  2446 LTTATTKSCTKTLDTCDYWPRCAACQHKSVSGELKKGPSASGKPLTVLDNVQPHVTSVIVNEDNA 2510
             ...:.:..:.||.:..:..........:|.|.::|....:             |.:.:|::.|
Zfish   357 -CAFSDEQSSVTLSSAYHTHTDPLLSVSAVQGTVRKAGRLA-------------VKNFLVHKKN- 406

  Fly  2511 VPGPQLRSLEKSNPSWTFLCSGIASICQPVQQTETDHSTAQEAA--EEDIVIRKV--KPNKSRRT 2571
             ...:|....|....|..|........:...::..||::..:.|  .|:.:::.|  .|.|....
Zfish   407 -KKVELAPRRKWKSYWVSLKGCTLFFYETDGRSGIDHNSVPKHAVWAENSIVQAVPEHPKKDFVF 470

  Fly  2572 GRSRSLPSVMLRRLYLAGMPKPGSNEM-----GTTSDCAASLSQQIKAVSSPTLSEPCLEEQPQK 2631
            ..|.||....|       .......|:     |..|.|||::::|.....:..|    |..:.:|
Zfish   471 CLSNSLGDSFL-------FQTCSQTELENWITGIHSACAAAVARQHHREDTVRL----LRAEIRK 524

  Fly  2632 GTESTDVANTGSSAGSGGLGVL---QKFKRTLNNF----NNKNQLH---------ISPLSPAAVG 2680
            ..:..|:.......|...|..:   :|.|..|...    .|..|.|         :|.|.    |
Zfish   525 LEQKIDMDEKMKKMGDMQLSAVTDAKKRKTILEQIFLWEQNLEQFHMDLFRFRCYLSSLQ----G 585

  Fly  2681 NNAPKTK------TSPTT---------TTASIAPMITTTTASD----PGSEAGDTSSGKYRFGPL 2726
            ...|..|      :.||.         :.:|...::...|.|.    ..:.:..||..|.||..|
Zfish   586 GELPNPKRLLAFASRPTKLAMGRLGIFSVSSFHALVAARTESGVRRRTQAMSRSTSKRKSRFSSL 650

  Fly  2727 IWRSSKERRKTK-YNRRDKC-NSGDSGIQIELE-QDEQYSRATMAVSR----------------- 2771
            ....:..:||:| :...|:. ..||..::..|| ..|..|..||..::
Zfish   651 WGLDTHSKRKSKGHPSIDQVFAEGDDPVKSPLEGMFEGSSSETMKDTKTPVKGLPRPNTDHDIWS 715

  Fly  2772 ------------QDEPRAFALTNGSCGVALSKMRAIRRTNSAKASS--ILGPFIVKTKIAKHLNI 2822
                        .|:|....:..|..  ||..:..|.:.:...|:.  :...|:::.::..::..
Zfish   716 RDHLTPSWVLLPNDQPVLAIIQPGES--ALDMLETICKAHQLDATKHYVRLKFLIENQVQFYIPK 778

  Fly  2823 GESDKAE---RKAPESLPTRSLSQPNGLES----YGMGRPDLEDSDSDSVASNEEAISFYPTTYA 2880
            .|.|..:   ::.......|.:.|.:..||    ||.         |.||...:.....|.|
Zfish   779 PEEDIYDLLYKEIELCCKIRKVIQFDRDESCMIGYGF---------SISVVEEDGVQQLYIT--- 831

  Fly  2881 EVLYNFTAGGPQELGLERGM-----LIEILRKEVGPWWFGRIK----KEETNLVEDILGP----E 2932
                :..|||   |...:|:     ::::..|:.....|..:|    :...:|..:.|.|    :
Zfish   832 ----DVRAGG---LAFAKGLNAGDEILQLNGKDSRTLTFADMKVAFAQVSLSLTVNTLPPVERRQ 889

  Fly  2933 LGWFPKEFVRIIHCPETDNFFIAHRAAADEAAAEEATARAEE---GSVPVPVAAFAEDTDVTVTT 2994
            |.:.|..     .....||.:....:.:.|...::......|   .|:......|||..|...:|
Zfish   890 LCYLPPR-----RSDAVDNLYTDIFSQSQEEILDDGVGLIVETSGDSLDDDSELFAEFDDYGKST 949

  Fly  2995 DQSNVTLIVIESPPTPSAPSVDFVAQPDHSTI--------------IRRSAVRELLDTEVNYVKL 3045
            :|.......:......|:.||...:.|:.|::              ..|..:.||::||..|||.
Zfish   950 EQVAAFCRSLHEMNPSSSESVSSSSVPESSSLPPSTATPRQLSDADKLRKVICELVETERTYVKD 1014

  Fly  3046 LAAICDGYLPAMSKRIDIFSPNSIRLIFSNITAIYKFQRKFLEALRRG---------IEQ-NQVA 3100
            |..:...||..:.|. ...:.:.:.::|.|:..:.:||.:||:.|..|         :|: .:..
Zfish  1015 LNCLIGRYLTPLQKE-SFLTQDELDVLFGNLPEMLEFQVEFLKTLEDGTRLVPDLEKLERVEEFK 1078

  Fly  3101 KVFLKMHKGFL-------CYSTYCNAY---PRALIELETYDRVKDA--RTILENCRESQNLAELP 3153
            |:...:...||       .||.:|.::   |:.|::.:|     ||  :..|:. |..:......
Zfish  1079 KILFSLGGSFLYYADRFKIYSAFCASHTKVPKVLVKAKT-----DAAFKAFLDE-RNPKQQHSST 1137

  Fly  3154 LSAHLLAPVQRICRYPLHLNEIIKSALEKGAKEGNDTDGAKSAGKTTDYEQLDVTELDIPDSQD- 3217
            |.::|:.|:||:.:|||.|.|:                     ...||           |||::
Zfish  1138 LESYLIKPIQRVLKYPLLLREL---------------------HSLTD-----------PDSEEH 1170

  Fly  3218 -TVNLALEAMRGITEAVNE-GKRHSETIARHQASFQNFKGPPLHLHSARFFLQV-DATRQKQN-- 3277
             .:|:|::||..:...:|| .|.|.|                   ..|.|.|.: :.|.:|:.
Zfish  1171 YHLNVAMKAMNTVASHINEMQKIHEE-------------------FGAVFDLLISEQTGEKKEVA 1216

  Fly  3278 -------LWNSSYTLFLFDNQLVYCKRDIIKRSHFIYK-GRIFLDRCRVVNVRDGKMFGHTIKNS 3334
                   |.:||.|.....:.|..||:: .:.:.|::| ..:|:  |:..:.:..||.|   .:.
Zfish  1217 DLSMGDLLLHSSVTWINPSSSLGKCKKE-PQLATFVFKTAVVFV--CKDYSKQKKKMGG---SHR 1275

  Fly  3335 LRIYSESRDKWYDFSFRSANRKHRFLSTLALE----RQFGGKALYVSEMTGFEYNYEERPGDYSD 3395
            ..:..|.:|   .|.||      ..:.|.||:    ....|::..|.|:...:...|.||
Zfish  1276 ASVSGEEKD---PFRFR------HMIPTDALQVRTLSNTDGESAAVCEIVHIKSESEGRP----- 1326

  Fly  3396 QSDYDAQDFELATGVTSGESSLPESPA------------KSTSRL--CETLPKKSQ--------- 3437
                 .:.|:|.       .|.|||..            |...:|  .|:||...|
Zfish  1327 -----ERVFQLC-------CSSPESKKDFLKTVHSILRDKQRRQLLKTESLPLSQQYVPFGGKRL 1379

  Fly  3438 -SRDGISSAENSQIVTTTSTGSLGRR 3462
             :..|...|.|..:...:.|  ||||
Zfish  1380 CALKGARPAMNRAVSAPSRT--LGRR 1403

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RhoGEF3	NP_612024.4	DUF4802	<754..>790	CDD:292678
		SH3	2876..2942	CDD:214620	14/78 (18%)
		RhoGEF	3031..3235	CDD:279015	52/227 (23%)
		PH_Collybistin_ASEF	3240..3373	CDD:269931	29/147 (20%)
		PH	<3285..3363	CDD:278594	15/78 (19%)
tiam1b	XP_009290217.1	PH1_Tiam1_2	387..513	CDD:269937	27/147 (18%)
		PH	400..502	CDD:278594	20/110 (18%)
		UBQ	721..796	CDD:294102	10/76 (13%)
		PDZ_signaling	799..879	CDD:238492	19/98 (19%)
		RhoGEF	1000..1189	CDD:214619	52/227 (23%)
		PH2_Tiam1_2	1191..1363	CDD:269957	44/222 (20%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3519
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.810