Protein Alignment NaCP60E and Cacna1h

DIOPT Version :10

• Sequence 1: NP_001261172.1
• Gene: NaCP60E / 37981
• FlyBaseID: FBgn0085434
• Length: 2896
• Species: Drosophila melanogaster
• Sequence 2: XP_006524796.1
• Gene: Cacna1h / 58226
• MGIID: 1928842
• Length: 2367
• Species: Mus musculus
• Alignment Length: 3016
• Identity: 641/3016 - (21%)
• Similarity: 1039/3016 - (34%)
• Gaps: 1122/3016 - (37%)

  Fly   116 RRVCVYIATNQFFDYCVMATILFNCIFLAMTETVEEAE--------------YIFLAIYSIEMVI 166
            |..|:.:..|.:|::..|..|:.||:.|.|....|:.|              :|| |.:::||||
Mouse    90 RSWCLRLVCNPWFEHISMLVIMLNCVTLGMFRPCEDVECRSERCSILEAFDDFIF-AFFAVEMVI 153

  Fly   167 KIIAKGFLLNKYTYLRNPWNWLDFVVITSG---YATIGMEVGNLAGLRTFRVLRALKTVSIMPGL 228
            |::|.| |..:..||.:.||.|||.::.:|   |:..|..| :|:.:||.||||.|:.::.:|.:
Mouse   154 KMVALG-LFGQKCYLGDTWNRLDFFIVMAGMMEYSLDGHNV-SLSAIRTVRVLRPLRAINRVPSM 216

  Fly   229 KTIINALLHSFRQLAEVMTLTIFCLMVFALFALQVYMGELRNKC------VRQ------VPTDWT 281
            :.::..||.:...|..|:.|..|...:|.:..:|::.|.|||:|      ||.      .|...|
Mouse   217 RILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLDSAFVRNNNLTFLRPYYQT 281

  Fly   282 NVSHTDWQIWVNDTDNWLY------DEDELPVLC--------------GNLTGARHC----PFEY 322
            .....:..|..:..||.:.      ...||.|.|              |. .|...|    .:..
Mouse   282 EEGEENPFICSSRRDNGMQKCSHIPSRRELRVQCTLGWEAYGQPQAEDGG-AGRNACINWNQYYN 345

  Fly   323 VCLCVGENPNHGYTNFDNFMWSMLTTFQLITLDYWENVYNMVLATCGPMSVSFFTVVVFFGSFYL 387
            ||.....||::|..||||..::.:..||:|||:.|.::...|:......:..:|.:::..|||::
Mouse   346 VCRSGEFNPHNGAINFDNIGYAWIAIFQVITLEGWVDIMYYVMDAHSFYNFIYFILLIIVGSFFM 410

  Fly   388 INLMLAVVALSYEE----EAEITNEERKKDLLDHRDDSTF-SFDPSVLNVKKLNK---NNKKKID 444
            |||.|.|:|..:.|    |.::..|:|.:.|   .:|||. ||.......::|.|   :..:|:.
Mouse   411 INLCLVVIATQFSETKQRENQLMREQRARYL---SNDSTLASFSEPGSCYEELLKYVGHIFRKVK 472

  Fly   445 SRKGVLLASYS---KKKT-----------RRKKTKGGKE------------------------GG 471
            .|...|.|.:.   :||.           ||:..:.|:.                        |.
Mouse   473 RRSLRLYARWQSRWRKKVDPSSTLHGQGPRRRPRRAGRRTASVHHLVYHHHHHHHHHYHFSHGGP 537

  Fly   472 TNGNGNGSNGDDNKSHSATPSPGPSPRHSATERPSALTM-----QAQKQYQQMEQQHKLA----- 526
            ...:.....||.....:..|...|||.|...:..|..::     ..:...::....|.:|
Mouse   538 RRPSPEPGAGDTRLVRACVPPSPPSPGHGPPDSESVHSIYHADCHVEGPQERARVAHTIATAASL 602

  Fly   527 --KSGSGGSNNP-MAPT------------PKGRISFQDSGMGVKNPNML-YPSDY-KGQLIANS- 573
              .||.|..|.| :.|:            |||..|....|....:|..| .||.| |.|.:...
Mouse   603 KLASGLGTMNYPTILPSGAVNSKGSTSSRPKGLRSAGTPGATAHSPLSLGSPSPYEKIQHVVGEQ 667

  Fly   574 --GQPSSNSSGVN-------------------------------RESSQDDSGVVDDHEEQDTTN 605
              |:.||:.||::                               .|.|..:||..|.|...:.|.
Mouse   668 GLGRASSHLSGLSVPCPLPSPQAGTLTCELKSCPYCASALEDPEFEFSGSESGDSDAHGVYEFTQ 732

  Fly   606 DMGHVSTVELALSPRE--------------VRLIKCNGNIARIKNHNVYALHQEFSSEVVVIDDL 656
            |:.|....:....|.|              :|.....|.:.|:
Mouse   733 DVRHGDCRDPVQQPHEGGTPGHGNERWRPPLRTASQPGGLGRL---------------------- 775

  Fly   657 PDRNCDRCVHWCTDYESWLQFQNCLYKVVRDPLFELAITLCIVLNTAFLAMEHHGMSESFRNALD 721
                             |..|.:.|.::|....|...|...|::||..:.:|:|...:...|||:
Mouse   776 -----------------WASFSSKLRRIVDSKYFNRGIMAAILVNTLSMGVEYHEQPDELTNALE 823

  Fly   722 VGNKVFTSIFTFECIVKLMALSKDFFLCG--------WNIFDLLIVTASLLDIIFELVDGLSVLR 778
            :.|.||||:|..|.::||:|       ||        :||||.::|..|:.:|:.:...||||||
Mouse   824 ISNIVFTSMFALEMLLKLLA-------CGPLGYIRNPYNIFDGIVVIISVWEIVGQADGGLSVLR 881

  Fly   779 GLRLLRVLKLAQSWTTMKVLLSIIISTIGALGNLTLILVIVIYIFAVIGMQLFSKDYTPEKFDPD 843
            ..||||||||.:....::..|.:::.|:..:....::|::.|:||:::||.||...::.:....|
Mouse   882 TFRLLRVLKLVRFLPALRRQLVVLMRTMDNVATFCMLLMLFIFIFSILGMHLFGCKFSLKTDSGD 946

  Fly   844 PVP-RWNFNDFFHSFMMIFRILCGE-WIEPLWDCMRAEEEQGASTCFAIFLPTLVMGNFMVLNLF 906
            .|| |.||:....:.:.:|:||..| |...|::.|.:.....|    ..|:..:..||:::.||.
Mouse   947 TVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSSWAA----LYFVALMTFGNYVLFNLL 1007

  Fly   907 LALLLNSFNSE--ELKSKKEEVGEESKLARSIERVRDLIRKKRQERKDRKERKFAEKFQQIVLDA 969
            :|:|:..|.:|  ..:|..:|....:.|....:::||:
Mouse  1008 VAILVEGFQAEGDATRSDTDEDKTSTHLEEDFDKLRDV--------------------------- 1045

  Fly   970 QQAHAQTLSHQAAVGLERGDKPGVLAETKFHRLSYQESMNRPVSGSDFGFQIPLHDGLHTIVDGL 1034
                      ||             .|.|.:.|:                          :....
Mouse  1046 ----------QA-------------TEMKMYSLA--------------------------VTPNG 1061

  Fly  1035 EYDDTGDLPEQI--QLQAHPLPPTSDSMPPTYESAMMATTGGSFSSVNGNGTCQNLTPFVQAERR 1097
            ..:..|.||..:  ...|.|:|                                  ||.......
Mouse  1062 HLEGRGSLPPPLITHTAATPMP----------------------------------TPKSSPHLD 1092

  Fly  1098 LQHQISSGVSTQQYDSREEATYTESIELLGQYNSTDTDPYANDQRSGCGSFNRGDSLQDNSSRRY 1162
            :.|.:        .|||..             :|...||...||:|..       ||:.:....:
Mouse  1093 MAHTL--------LDSRRS-------------SSGSVDPQLGDQKSLA-------SLRSSPCAPW 1129

  Fly  1163 GSEEHDEAFLKYQKSLLTRSPSYRKSLDRLSQSSGQSQRSLLKSEEAEMRRHSSGQSLNSMSIEQ 1227
            |                             ..|:|.|:||                |.||:
Mouse  1130 G-----------------------------PNSAGSSRRS----------------SWNSL---- 1145

  Fly  1228 DELLSQQGNLREELLNCDQKELFQFLQEEEELQKGTKLRRISNVMRSRRPSSQMGQPENETMVEH 1292
                                                          .|.||
Mouse  1146 ----------------------------------------------GRAPS-------------- 1150

  Fly  1293 SEFDNIIQSFEKELEEIKRSTTSLERKLSNLSEPSPAADEATKAIMEHIAIITGASERSAADEVV 1357
                            :||.:...||:                      ::::|..:.|..||.
Mouse  1151 ----------------LKRRSQCGERE----------------------SLLSGEGKGSTDDEA- 1176

  Fly  1358 LPLNPYDSYDLSSVPRRSQSVSAAAQRQSVKLKRRSLEKQRKIDEDFSISNEIRKICDQIHAPFV 1422
                                                        ||
Mouse  1177 --------------------------------------------ED------------------- 1178

  Fly  1423 AMEAMAVAATSASQAQPNQSPFLRRKVDPFTVQFDRFKRLSLIERVEEVPEEEKPISTLRIESEK 1487
                    :...|...|..||..|                               .:.||
Mouse  1179 --------SRPNSGTHPGASPGPR-------------------------------ATPLR----- 1199

  Fly  1488 MPRKFLHGPDQLRLDSLSLKSTNSYENLLIQKQKLGMATPPAVPAT-PPTSLKSSIEPPTLAQIS 1551
                        |.:||..:||              |...|..||| .||.
Mouse  1200 ------------RAESLGHRST--------------MDLCPPRPATLLPTK-------------- 1224

  Fly  1552 SLKTTPPLAALTEHQQHFHATSIQAAPTPAHTHAHSQAHAHSMAGQRRRMEHPQSTLDKAASFQS 1616
                             |...:.|....|:...
Mouse  1225 -----------------FRDCNGQMVALPSEFF-------------------------------- 1240

  Fly  1617 ARTESHSSGAADASSALALAMAQKTEQSQSTAPDATQKPSAFTRLTEKPWHCLVSYVDDLTVGGR 1681
            .|.:||...||:                                           :.||:
Mouse  1241 LRIDSHKEDAAE-------------------------------------------FDDDI----- 1257

  Fly  1682 RNSQGAYNDPMTFPSYGATKAAKVPDDCFPQKCYDHFYFRCPWFMSCMDTQSAKHWTRVRTAVLT 1746
                   .|...|      :..||.:...||.|..    |..|.:.....|:     |:|.:...
Mouse  1258 -------EDSCCF------RLHKVLEPYAPQWCSS----RESWALYLFPPQN-----RLRVSCQK 1300

  Fly  1747 VVDTPAFEWFVLVLIFASSITLCFEDINLDKNKTLKRVLYWINFSFCLIFVVEMILKWLALGF-- 1809
            |:....|:..|||.||.:.||:..|..::|...|.:..|...|:.|..||||||::|.:|||.
Mouse  1301 VIAHKMFDHVVLVFIFLNCITIALERPDIDPGSTERAFLSVSNYIFTAIFVVEMMVKVVALGLLW 1365

  Fly  1810 --SKYFTSFWTILDFIIVFVSVFSLLIE-----ENENLKVLRSLRTLRALRPLRAISRWQGMRIV 1867
              ..|..|.|.:||.::|.||:..:::.     ..:.|.|||.||.||.|||||.|||..|:::|
Mouse  1366 GEHAYLQSSWNVLDGLLVLVSLVDIIVAVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLV 1430

  Fly  1868 VNALMYAIPSIFNVLLVCLVFWLIFSIMGVQFFGGKFFKCVNEMGELLPITEVNDKWDCIEQNYT 1932
            |..|:.::..|.|::|:|..|::||.|:|||.|.|||:.|     |......:..|.:|...:|.
Mouse  1431 VETLISSLRPIGNIVLICCAFFIIFGILGVQLFKGKFYYC-----EGTDTRNITTKAECHAAHYR 1490

  Fly  1933 WINSKITFDHVGMGYLALLQVATFEGWMEVMADAVDARGVDLQPQREANLYAYIYFVIFIVCGSF 1997
            |:..|..||::|...::|..:::.:||:.:|.|.:||.|:|.||.:..|.:..:||:.|::..||
Mouse  1491 WVRRKYNFDNLGQALMSLFVLSSKDGWVNIMYDGLDAVGIDQQPVQNHNPWMLLYFISFLLIVSF 1555

  Fly  1998 FTLNLFIGVIIDNFNMLKKKYEGGVLEMFLTESQKHYYTAMKKLGRKKPQKVI-------KRP-- 2053
            |.||:|:||:::||:..::..|.       .|:::.....:::|.|::..|.:       :||
Mouse  1556 FVLNMFVGVVVENFHKCRQHQEA-------EEARRREEKRLRRLERRRRSKAVPEVEEAQRRPYY 1613

  Fly  2054 --INHFLAMFYDLSNSRRFEIAIFVLIFLNMLTMGIEHYDQPHAVFFILEVSNAFFTTVFGLEAI 2116
              .:|.....:.|..|...::.|..:|.||::||.:|||:||.::...|:..|..||.||..||.
Mouse  1614 ADYSHTRRSIHSLCTSHYLDLFITFIICLNVITMSMEHYNQPKSLDEALKYCNYVFTIVFVFEAA 1678

  Fly  2117 VKIVGLRY-HYFTVPWNVFDFLLVLASIFGILMEDIMID--LPISPTLLRVVRVFRIGRILRLIK 2178
            :|:|...: .:|...||..|..:||.||.||.:|:|.::  |||:||::|::||.||.|:|:|:|
Mouse  1679 LKLVAFGFRRFFKDRWNQLDLAIVLLSIMGIALEEIEMNAALPINPTIIRIMRVLRIARVLKLLK 1743

  Fly  2179 AAKGIRKLLFALVVSLPALFNIGALLGLITFIYAILGMSLFGNVKLQ-----GALDDMVNFQTFG 2238
            .|.|:|.||..:|.:||.:.|:|.|..|:.||||.||:.|||.::..     ..|.....|..||
Mouse  1744 MATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGRLECSEDNPCEGLSRHATFTNFG 1808

  Fly  2239 RSMQLLFRLMTSAGWN----DVLESLMIQPPDCDPFIHGHTNGNCGHPLLAITYFTSFIIISYMI 2299
            .:...|||:.|...||    |.|.....:...|..::          |.|:..||.:|::::..:
Mouse  1809 MAFLTLFRVSTGDNWNGIMKDTLRECTREDKHCLSYL----------PALSPVYFVTFVLVAQFV 1863

  Fly  2300 VINMYIAIILENFNQAHQE-EEIGIVEDDLEMFYIRWSKYDPHATQFIHFSQLSDFIASLDPPL- 2362
            ::|:.:|:::::..::::| .|...::.::|:...:.|...|.:|     :|.|.......|.|
Mouse  1864 LVNVVVAVLMKHLEESNKEAREDAEMDAEIELEIAQGSTAQPPST-----AQESQGTEPDTPNLL 1923

  Fly  2363 ---------GISKPNNVALVSFNLPISKGNKIHCLDILHALVKHVLGHVE-ETDNFKQLQEQMDV 2417
                     .:|.||:..:..   |::.....|         .|.|..|| ||
Mouse  1924 VVRKVSVSRMLSLPNDSYMFR---PVAPAAAPH---------SHPLQEVEMET------------ 1964

  Fly  2418 KFKKQFPTRKELEIVSSTRIWKRQEKAAKTIQTGWKEYLRRKREKERSNSGDSATQTSSPGGWQS 2482
                                           .||                  ..|...||
Mouse  1965 -------------------------------YTG------------------PVTSAHSP----- 1975

  Fly  2483 KLSALNFFHLQVSRRGTACSSRASSRKSSRASDASDLSELAGPWLNLPLMLVSGADEVVKDIKQQ 2547
                             :...|.|.:..|.||..:.:|:        ||..:|..|
Mouse  1976 -----------------SLEPRTSFQVPSAASSPARVSD--------PLCALSPRD--------- 2006

  Fly  2548 NDELGKRGSIFVEAPRA-------SRRRSFYNFFLRHQ--DAVDDSL---TSPSVHRKTAMNNTT 2600
                         .||:       .|:.:.:...|..|  ||.:||:   |.|:
Mouse  2007 -------------TPRSLSLSRILCRQEAMHAESLEGQIDDAGEDSIPDYTEPA----------- 2047

  Fly  2601 NTTSNSASTSGTASSTATAPATGCGPAATSASDSDRHQAVGGGSAPSRKRASSF----IRKKPPL 2661
            ...|.|.:..||..|..      |.|...|.                |.|..:|    |..:||.
Mouse  2048 ENISMSQAPLGTLRSPP------CSPRPASV----------------RTRKHTFGQHCISSRPPT 2090

  Fly  2662 ERGLSAQSALRVNKNAFVSEASAPEVIVTRPSPEQQTHPHSLSLRPDNATLVHVLVHRESEEYKE 2726
            ..|..|::|                    .|:.|:.:|..| |..|..||..|
Mouse  2091 LGGDDAEAA--------------------DPADEEVSHITS-SAHPWPATEPH------------ 2122

  Fly  2727 EDESSPSVSGNGNGFGVGLDMLSKQPPPQIRITTGSVESSMDT---CAMPTVQIMVDSPKDPPRG 2788
            ..|:||:.|                 |.:     |:|.|..|.   |:: ..|..:|.|..|...
Mouse  2123 SPEASPTAS-----------------PAK-----GTVGSGRDPHRFCSV-DAQSFLDKPGRPDAQ 2164

  Fly  2789 DFSSAPIDDVGAPIDVNVQGDTSQVFYDYNP-----------------EKATDDQGNGQDETAQF 2836
            .:||..:|:....::   .|:......:..|                 :..|:|:|:.:...|:.
Mouse  2165 RWSSVELDNGDGHLE---SGEVRARASELEPALGARRKKKMSPPCISIDPPTEDEGSSRPPAAEG 2226

  Fly  2837 ESLPDRQRXPPVNTPLRDSEKRSSRG 2862
            .:...|:| |.....|......|:.|
Mouse  2227 GNTTLRRRTPSCEAALHRDCPESTEG 2252

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NaCP60E	NP_001261172.1	Ion_trans	125..371	CDD:459842	84/298 (28%)
		Ion_trans	687..902	CDD:459842	70/224 (31%)
		Ion_trans	1751..2019	CDD:459842	102/276 (37%)
		Na_channel_gate	2009..2063	CDD:240441	10/64 (16%)
		Ion_trans	2067..2320	CDD:459842	90/265 (34%)
		GPHH	2332..2378	CDD:465306	10/55 (18%)
Cacna1h	XP_006524796.1	Ion_trans	99..430	CDD:459842	95/334 (28%)
		Ion_trans	789..1023	CDD:459842	76/244 (31%)
		KLF9_13_N-like	<1056..1200	CDD:425361	49/498 (10%)
		Ion_trans	1304..1576	CDD:459842	102/276 (37%)
		Ion_trans	1629..1860	CDD:459842	87/240 (36%)
		PHA03247	<1945..2360	CDD:223021	89/522 (17%)