Protein Alignment NaCP60E and scn8aa

DIOPT Version :10

• Sequence 1: NP_001261172.1
• Gene: NaCP60E / 37981
• FlyBaseID: FBgn0085434
• Length: 2896
• Species: Drosophila melanogaster
• Sequence 2: XP_068072894.1
• Gene: scn8aa / 58152
• ZFINID: ZDB-GENE-000828-1
• Length: 1960
• Species: Danio rerio
• Alignment Length: 2651
• Identity: 812/2651 - (30%)
• Similarity: 1172/2651 - (44%)
• Gaps: 894/2651 - (33%)

  Fly    45 PRTKASVEDGDVLPRKFEPFPEHMYGKPLEEIDTF-IYEETFCVVSKRFRKNYIHRFTGTKSLFL 108
            |:....:|.|..||..:...|..|...|||:.|.| |.::||.|::|   ...|.||:.|.:|::
Zfish    53 PKPNGDLEAGKSLPFIYGDIPPGMVATPLEDFDPFYINQKTFVVLNK---GKTIFRFSATPALYM 114

  Fly   109 FYPWSPARRVCVYIATNQFFDYCVMATILFNCIFLAMT---ETVEEAEYIFLAIYSIEMVIKIIA 170
            ..|::.|||:.:.|..:..|...:|.|||.||:|:..:   |..::.||.|..||:.|..:||||
Zfish   115 ISPFNLARRIAIKILIHSVFSMFIMCTILTNCVFMTFSNPPEWSKQVEYTFTGIYTFESAVKIIA 179

  Fly   171 KGFLLNKYTYLRNPWNWLDFVVITSGYATIGMEVGNLAGLRTFRVLRALKTVSIMPGLKTIINAL 235
            :||.::.:|:||:|||||||:||:..|.|..:::||::.||||||||||||:|::||||||:.||
Zfish   180 RGFCIDGFTFLRDPWNWLDFMVISMAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGAL 244

  Fly   236 LHSFRQLAEVMTLTIFCLMVFALFALQVYMGELRNKCV------RQVPTDWTNVSHTDWQIWVND 294
            :.|.::|::||.||:|||.||||..||::||.||.|||      .::..|.......:|..::.:
Zfish   245 IQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRQKCVIWPINITEMFPDVNGSRAFNWDEYILN 309

  Fly   295 TDNWLYDEDEL-PVLCGNLTGARHCPFEYVCLCVGENPNHGYTNFDNFMWSMLTTFQLITLDYWE 358
            ..|:.:..|:| .:||||.:.:..||..|.|:..|.|||:|||:||:|.|:.|..|:|:|.|:||
Zfish   310 ETNFYFLPDQLDALLCGNSSDSGRCPEGYTCMKAGRNPNYGYTSFDSFGWAFLALFRLMTQDFWE 374

  Fly   359 NVYNMVLATCGPMSVSFFTVVVFFGSFYLINLMLAVVALSYEEEAEITNEERKKDLLDHRDDSTF 423
            |:|.:.|...|...:.||.:|:|.|||||:||:|||||::|||:.:.|.||.:      |.::.|
Zfish   375 NLYQLTLRAAGKTYMIFFVLVIFVGSFYLVNLILAVVAMAYEEQNQATMEEAE------RKEAEF 433

  Fly   424 SFDPSVLNVKKLNKNNKKKIDSRKGVLLASYSKKKTRRKKTKGGKEGGT--------NGNGNGSN 480
            .        ..|.:..|::.|::...:..|                .||        :|:|.|
Zfish   434 K--------AMLEQLKKQQEDAQANAMATS----------------AGTVSEDVVEDDGDGEG-- 472

  Fly   481 GDDNKSHSATPSPGPSPRHSATERPSALTMQAQKQYQQMEQ------QHKLAKSGSGGSNNPMAP 539
               |.|.|::.....|.: ||.||     ...:|:::|.||      ..|..||.|...:
Zfish   473 ---NLSCSSSEMSKLSSK-SAKER-----RNRKKKWRQKEQDKEKGDSEKFVKSESDDGS----- 523

  Fly   540 TPKGRISFQDSGMGVKNPNMLYPSDYKGQLIANSGQP-----SSNSS--------GVNRESSQDD 591
              :.|..|.|:.:|.::..|      ...|::..|.|     :|.||        |...|.:.|:
Zfish   524 --RRRFRFPDNRLGRRSSIM------NQSLLSIPGSPFPSRRNSKSSIFSRCKDGGSENEFADDE 580

  Fly   592 SGVVDDHEEQ---------------------DTTNDMGHVSTV----------ELAL-------S 618
            ...|::::|:                     .|.:..|.||.:          |:.:       |
Zfish   581 HSTVEEYDERRDSFLSPQRRSSYTGFYGKRNSTVDCNGVVSLIGPGPGGRLLPEVIIDKAATDDS 645

  Fly   619 PR---EVRLIKCNGNI-ARIKNHNVYALHQEFSSEVV------VIDDLPD--RNCDRCVH----- 666
            |.   |::. |.:|:: ..::..|.....:|.::.|:      ::::|.:  |.|..|.:
Zfish   646 PTTDLEIKK-KLSGSLMVSVEQLNTSFGRKERANSVMSALTNTLVEELEESQRKCPPCWYKFANT 709

  Fly   667 ---W-CTDYESWLQFQNCLYKVVRDPLFELAITLCIVLNTAFLAMEHHGMSESFRNALDVGNKVF 727
               | |...  |:..:..:..:|.||..:||||:||||||.|:||||:.|:..|.:.|.|||.||
Zfish   710 FLIWECGPL--WMSIKEIVNLIVMDPFVDLAITICIVLNTLFMAMEHYPMTPQFEHVLSVGNLVF 772

  Fly   728 TSIFTFECIVKLMALSK-DFFLCGWNIFDLLIVTASLLDIIFELVDGLSVLRGLRLLRVLKLAQS 791
            |.|||.|...||:|:.. .:|..||||||..||:.||:::....|:||||||..|||||.|||:|
Zfish   773 TGIFTAEMFAKLVAMDPYYYFQEGWNIFDGFIVSLSLMELGLANVEGLSVLRSFRLLRVFKLAKS 837

  Fly   792 WTTMKVLLSIIISTIGALGNLTLILVIVIYIFAVIGMQLFSKDYTP--EKFDPD-PVPRWNFNDF 853
            |.|:.:|:.||.:::||||||||:|.|:::||||:|||||.|.|..  .|...| .:|||:.|||
Zfish   838 WPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIAQDCELPRWHMNDF 902

  Fly   854 FHSFMMIFRILCGEWIEPLWDCMRAEEEQGASTCFAIFLPTLVMGNFMVLNLFLALLLNSFNSEE 918
            ||||:::||:|||||||.:||||   |..|.:.|..:|:..:|:||.:||||||||||:||:::.
Zfish   903 FHSFLIVFRVLCGEWIETMWDCM---EVAGQAMCLIVFMMVMVIGNLVVLNLFLALLLSSFSADN 964

  Fly   919 LKSKKEEVGEESKLARSIER-----------VRDLI-----RKKRQERK---DRKERKFAEKFQQ 964
            |.:..:: ||.:.|..::.|           ||:|:     ||...|.|   |..:||.
Zfish   965 LAASDDD-GEMNNLQIAVIRIKKGIAWVKAKVRELVNIILGRKVTDEAKPLDDMYDRKL------ 1022

  Fly   965 IVLDAQQAHAQTLSHQAAVGLERGDKPGVLAETKFHRLSYQESMNRPVSGSDFGFQIPLHDGLHT 1029
                      ..:::...|.:.|.             |.||:
Zfish  1023 ----------NCIANHTGVDISRD-------------LDYQK----------------------- 1041

  Fly  1030 IVDGLEYDDTGDLPEQIQLQAHPLPPTSDSMPPTYESAMMATTGGSFSSVNGNGTCQNLTPFVQA 1094
                                                              |||||
Zfish  1042 --------------------------------------------------NGNGT---------- 1046

  Fly  1095 ERRLQHQISSGVSTQQYDSREEATYTESIELLGQYNSTDTDPYANDQRSGCGSFNRGDSLQDNSS 1159
                    :||:
Zfish  1047 --------TSGI----------------------------------------------------- 1050

  Fly  1160 RRYGSEEHDEAFLKYQKSLLTRSPSYRKSLDRLSQSSGQSQRSLLKSEEAEMRRHSSGQSLNSMS 1224
                                                                     |.|:....
Zfish  1051 ---------------------------------------------------------GSSVGKYM 1058

  Fly  1225 IEQDELLSQQGNLREELLNCDQKELFQFLQEEEELQKGTKLRRISNVMRSRRPSSQMGQPENETM 1289
            |:.|.:                    .|:                     ..|:..:..|   ..
Zfish  1059 IDDDHM--------------------SFI---------------------HNPNLTVCVP---IA 1079

  Fly  1290 VEHSEFDNI-IQSFEKELEEIKRSTTSLERKLSNLSEPSPAADEATKAIMEHIAIITGASERSAA 1353
            |..|:|:|: .:.|..|.|                :|.|...|:.:            :||.|..
Zfish  1080 VGESDFENLNTEDFSSESE----------------AEGSKELDDIS------------SSEGSTI 1116

  Fly  1354 DEVVLPLNPYDSYDLSSVPRRSQSVSAAAQRQSVKLKRRSLEKQRKIDEDFSISNEIRKICDQIH 1418
            |                                                   |..|:.
Zfish  1117 D---------------------------------------------------IKPEVE------- 1123

  Fly  1419 APFVAMEAMAVAATSASQAQPNQSPFLRRKVDPFTVQFDRFKRLSLIERVEEVPEEEKPISTLRI 1483
                  ||:                                    ::|.|||
Zfish  1124 ------EAV------------------------------------VVETVEE------------- 1133

  Fly  1484 ESEKMPRKFLHGPDQLRLDSLSLKSTNSYENLLIQKQKLGMATPPAVPATPPTSLKSSIEPPTLA 1548
                                                                             
Zfish  1134 ----------------------------------------------------------------- 1133

  Fly  1549 QISSLKTTPPLAALTEHQQHFHATSIQAAPTPAHTHAHSQAHAHSMAGQRRRMEHPQSTLDKAAS 1613
                                                                             
Zfish  1134 ----------------------------------------------------------------- 1133

  Fly  1614 FQSARTESHSSGAADASSALALAMAQKTEQSQSTAPDATQKPSAFTRLTEKPWHCLVSYVDDLTV 1678
                                                                      |||
Zfish  1134 ----------------------------------------------------------YVD---- 1136

  Fly  1679 GGRRNSQGAYNDPMTFPSYGATKAAKVPDDCFPQKCYDHFYFRCPWFMSCMD---TQS-AKHWTR 1739
                                       |:.|:.:.|...:        .|.|   |:. .|:|..
Zfish  1137 ---------------------------PEACWTEACIARY--------KCCDVPITEGWGKNWWF 1166

  Fly  1740 VRTAVLTVVDTPAFEWFVLVLIFASSITLCFEDINLDKNKTLKRVLYWINFSFCLIFVVEMILKW 1804
            :|.....:|:...||..::.:|..||..|.|||:.:::.||::.:|.:.:..|..||::||:|||
Zfish  1167 LRKTCYLIVEHNWFETLIIFMILLSSGALAFEDVYIEQRKTIRIILEYADMVFTYIFILEMLLKW 1231

  Fly  1805 LALGFSKYFTSFWTILDFIIVFVSVFSLLIEE--NENLKVLRSLRTLRALRPLRAISRWQGMRIV 1867
            :|.||.||||:.|..|||.||.||:.||:...  ..:|..::|||||||||||||:||::|||:|
Zfish  1232 VAYGFVKYFTNAWCWLDFFIVDVSIVSLIANALGYSDLGPIKSLRTLRALRPLRALSRFEGMRVV 1296

  Fly  1868 VNALMYAIPSIFNVLLVCLVFWLIFSIMGVQFFGGKFFKCVNEMGE-LLPITEVNDKWDC---IE 1928
            ||||:.|||||.|||||||:||||||||||..|.||::.|.||..: ...:..||:|.:|   ||
Zfish  1297 VNALVGAIPSIMNVLLVCLIFWLIFSIMGVNMFAGKYYYCYNETEKAYFELDVVNNKSECFALIE 1361

  Fly  1929 QNYT---WINSKITFDHVGMGYLALLQVATFEGWMEVMADAVDARGVDLQPQREANLYAYIYFVI 1990
            ||||   |.|.||.||:||.|||||||||||:|||::|..|||:|.|:.||:.|.|:|.||||||
Zfish  1362 QNYTEVRWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRRVEDQPKYEDNIYMYIYFVI 1426

  Fly  1991 FIVCGSFFTLNLFIGVIIDNFNMLKKKYEGGVLEMFLTESQKHYYTAMKKLGRKKPQKVIKRPIN 2055
            ||:.||||||||||||||||||..|||:.|  .::|:||.||.||.||||||.|||||.|.||.|
Zfish  1427 FIIFGSFFTLNLFIGVIIDNFNQQKKKFGG--QDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPQN 1489

  Fly  2056 HFLAMFYDLSNSRRFEIAIFVLIFLNMLTMGIEHYDQPHAVFFILEVSNAFFTTVFGLEAIVKIV 2120
            ....|.:|....:.|:|:|.:||.|||:||.:|..||......||...|..|...|..|.::|:.
Zfish  1490 KLQGMVFDFVTQQVFDISIMILICLNMVTMMVETDDQSQETENILYWINFIFIVAFTSEFVLKLF 1554

  Fly  2121 GLRYHYFTVPWNVFDFLLVLASIFGILMEDIMIDLPISPTLLRVVRVFRIGRILRLIKAAKGIRK 2185
            .||::|||..||:||.::|:.||.|:.:.|::....:||||.||:|:.||||||||||.|||||.
Zfish  1555 ALRHYYFTNGWNIFDCVVVILSIVGMFLADLIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRT 1619

  Fly  2186 LLFALVVSLPALFNIGALLGLITFIYAILGMSLFGNVKLQGALDDMVNFQTFGRSMQLLFRLMTS 2250
            |||||::|||||||||.||.|:.||::|.|||.|..||.:..:|||.||:|||.||..||.:.||
Zfish  1620 LLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYVKRESGIDDMYNFETFGNSMICLFMITTS 1684

  Fly  2251 AGWNDVLESLMIQPPDCDPFIHG---HTNGNCGHPLLAITYFTSFIIISYMIVINMYIAIILENF 2312
            |||:.:|..::..||||:|....   ...||||:|.:.|.:|..:||:|::||:|||||||||||
Zfish  1685 AGWDGLLAPILNYPPDCEPTKENPGTSVKGNCGNPSVGIFFFVMYIIVSFLIVVNMYIAIILENF 1749

  Fly  2313 NQAHQEEEIGIVEDDLEMFYIRWSKYDPHATQFIHFSQLSDFIASLDPPLGISKPNNVALVSFNL 2377
            :.|.:|....:.|||.|.||..|.|:||.|:|||.|::|.||..:|:.||.:.|||.:.|::.:|
Zfish  1750 SVATEESADPLCEDDFESFYEIWEKFDPTASQFITFAKLGDFADTLEHPLRVPKPNTIELIAMDL 1814

  Fly  2378 PISKGNKIHCLDILHALVKHVLGHVEETDNFKQLQEQMDVKFKKQFPTRKELEIVSSTRIWKRQE 2442
            |:..|::|||||||.|..|.|||   ::.:...:::||:.:|....|::...|.:::|...|::.
Zfish  1815 PMVSGDRIHCLDILFAFTKRVLG---DSGDLDMMRQQMEERFIAANPSKVSFEPITTTLRRKQEH 1876

  Fly  2443 KAAKTIQTGWKEYLRRKREKERSNSGDSATQTSSPGGWQSKLSALNFFHLQVSRRGTACSSRASS 2507
            .:|..||..::.:|.||                                      |..|....||
Zfish  1877 MSAGVIQRAFRAHLIRK--------------------------------------GFICKRLLSS 1903

  Fly  2508 RKSSRASDASDLSELAGPWLNLPLMLVSGADEVVKDIK---QQNDELGKRG 2555
            .:........|..|......:||     ..|.|.|..|   :::|..||:|
Zfish  1904 SRLENGGTNQDKKEGTPSTASLP-----SYDSVTKPEKEKLEESDSKGKKG 1949

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NaCP60E	NP_001261172.1	Ion_trans	125..371	CDD:459842	115/255 (45%)
		Ion_trans	687..902	CDD:459842	118/218 (54%)
		Ion_trans	1751..2019	CDD:459842	161/276 (58%)
		Na_channel_gate	2009..2063	CDD:240441	31/53 (58%)
		Ion_trans	2067..2320	CDD:459842	130/255 (51%)
		GPHH	2332..2378	CDD:465306	20/45 (44%)
scn8aa	XP_068072894.1	Ion_trans	131..423	CDD:459842	134/291 (46%)
		ARGLU	<416..>448	CDD:405931	10/45 (22%)
		Na_trans_cytopl	544..682	CDD:463401	24/138 (17%)
		Ion_trans	732..945	CDD:459842	116/215 (54%)
		Na_trans_assoc	971..1172	CDD:461936	67/843 (8%)
		Ion_trans	1177..1453	CDD:459842	159/275 (58%)
		Na_channel_gate	1445..1497	CDD:240441	31/53 (58%)
		Ion_trans	1502..1756	CDD:459842	129/253 (51%)