DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment NaCP60E and Cacna1c

DIOPT Version :9

Sequence 1:	NP_001261172.1	Gene:	NaCP60E / 37981	FlyBaseID:	FBgn0085434	Length:	2896	Species:	Drosophila melanogaster
Sequence 2:	NP_036649.2	Gene:	Cacna1c / 24239	RGDID:	2245	Length:	2170	Species:	Rattus norvegicus

Alignment Length:	3036	Identity:	613/3036 - (20%)
Similarity:	978/3036 - (32%)	Gaps:	1320/3036 - (43%)

- Green bases have known domain annotations that are detailed below.

  Fly    69 YGKPLEEIDTFIYEETFCVVSKRFRKNYIHRFTGT---KSLFLFYPWSPARRVCVYIATNQFFDY 130
            ||||.::..|                      |.|   ::|......:|.||.|:.|...:.|:.
  Rat   116 YGKPKKQGGT----------------------TATRPPRALLCLTLKNPIRRACISIVEWKSFEI 158

  Fly   131 CVMATILFNCIFLAM------------TETVEEAEYIFLAIYSIEMVIKIIAKGFLLNKYTYLRN 183
            .::.||..||:.||:            ...:|..||:||.|:::|..:|:||.|.|.:...||||
  Rat   159 IILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRN 223

  Fly   184 PWNWLDFVVITSGYATIGMEV-----------GNLAG-----LRTFRVLRALKTVSIMPGLKTII 232
            .||.|||:::..|..:..:|.           |..||     ||.|||||.|:.||.:|.|:.::
  Rat   224 GWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVL 288

  Fly   233 NALLHSFRQLAEVMTLTIFCLMVFALFALQVYMGELRNKCVRQ-----VPTDWTNVSHTDWQIWV 292
            |:::.:...|..:..|.:|.::::|:..|:::||::...|..|     ||.
  Rat   289 NSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIIDVPA-------------- 339

  Fly   293 NDTDNWLYDEDELPVLCGNLTG-ARHCPFEYVCLCVGENPNHGYTNFDNFMWSMLTTFQLITLDY 356
                    :||..|  |...|| .|.|....||....:.|.||.||||||.::|||.||.||::.
  Rat   340 --------EEDPSP--CALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEG 394

  Fly   357 WENV-YNMVLATCGPMSVSFFTVVVFFGSFYLINLMLAVVALSYEEEAEITNEERKKDLLDHRDD 420
            |.:| |.|..|....:...:|..:|.||||:::||:|
  Rat   395 WTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVL---------------------------- 431

  Fly   421 STFSFDPSVLNVKKLNKNNKKKIDSRKGVLLASYSKKKTRRKKTKGGKEGGTNGNGNGSNGDDNK 485
                                       |||...:||::.:.|                :.||
  Rat   432 ---------------------------GVLSGEFSKEREKAK----------------ARGD--- 450

  Fly   486 SHSATPSPGPSPRHSATERPSALTMQAQKQYQQMEQQHK-----LAKSGSGGSNNPMAPTPKGRI 545
                                    .|..::.||:|:..|     :.::......|
  Rat   451 ------------------------FQKLREKQQLEEDLKGYLDWITQAEDIDPEN---------- 481

  Fly   546 SFQDSGMGVKNPNMLYPSDYKGQLIANSGQPSSNSSGVNRESSQDDSGVVDDHEEQDTTNDMGHV 610
              :|.||....|.             |...|:|.:..||.|:
  Rat   482 --EDEGMDEDKPR-------------NMSMPTSETESVNTEN----------------------- 508

  Fly   611 STVELALSPREVRLIKCNGNIARIKNHNVYALHQEFSSEVVVIDDLPDRNCDRCVHWCTDYESWL 675
                  ::..::....|...:|    |.:  ...:||.                 :|    ..|.
  Rat   509 ------VAGGDIEGENCGARLA----HRI--SKSKFSR-----------------YW----RRWN 540

  Fly   676 QFQNCLYK---VVRDPLFELAITLCIVLNTAFLAMEHHGMSESFRNALDVGNKVFTSIFTFECIV 737
            :|  |..|   .|:..:|...:...:.|||..:|.||:..........|..||...::||.|.::
  Rat   541 RF--CRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLL 603

  Fly   738 KLMALS-KDFFLCGWNIFDLLIVTASLLDIIFELVD-------GLSVLRGLRLLRVLKLAQSWTT 794
            |:.:|. :.:|:..:|.||..||...:|:.|  ||:       |:||||.:||||:.|:.:.|.:
  Rat   604 KMYSLGLQAYFVSLFNRFDCFIVCGGILETI--LVETKIMSPLGISVLRCVRLLRIFKITRYWNS 666

  Fly   795 MKVLLSIIISTIGALGNLTLILVIVIYIFAVIGMQLFSKDYTPEKFDPDPVPRWNFNDFFHSFMM 859
            :..|::.:::::.::.:|.|:|.:.|.||:::|||||...:   .||.....|..|::|..|.:.
  Rat   667 LSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKF---NFDEMQTRRSTFDNFPQSLLT 728

  Fly   860 IFRILCGE-WIEPLWDCMRA---EEEQGASTCFAIFLPTLVMGNFMVLNLFLALLL-NSFNSEEL 919
            :|:||.|| |...::|.:.|   ....|...|. .|:...:.||:::||:|||:.: |..::|.|
  Rat   729 VFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCI-YFIILFICGNYILLNVFLAIAVDNLADAESL 792

  Fly   920 KSKKEEVGEESKLARSIERVRDLIRKKRQERKDRKERKFAEKFQQIVLDAQQAHAQTLSHQAAVG 984
            .|.::|..||                  :|||........||.|:::
  Rat   793 TSAQKEEEEE------------------KERKKLARTASPEKKQEVM------------------ 821

  Fly   985 LERGDKPGVLAETKFHRLSYQESMNRPVSGSDFGFQIPLHDGLHTIVDGLEYDDTGDLPEQIQLQ 1049
                :||.|
  Rat   822 ----EKPAV-------------------------------------------------------- 826

  Fly  1050 AHPLPPTSDSMPPTYESAMMATTGGSFSSVNGNGTCQNLTPFVQAERRLQHQISSGVSTQQYDSR 1114

  Rat   827 ----------------------------------------------------------------- 826

  Fly  1115 EEATYTESIELLGQYNSTDTDPYANDQRSGCGSFNRGDSLQDNSSRRYGSEEHDEAFLKYQKSLL 1179
             |.:..|.|||
  Rat   827 -EESKEEKIEL------------------------------------------------------ 836

  Fly  1180 TRSPSYRKSLDRLSQSSGQSQRSLLKSEEAEMRRHSSGQSLNSMSIEQDELLSQQGNLREELLNC 1244
                   ||:.                        :.|:|..:..|..|:|
  Rat   837 -------KSIT------------------------ADGESPPTTKINMDDL-------------- 856

  Fly  1245 DQKELFQFLQEEEELQKGTKLRRISNVMRSRRPSSQMGQPENETMVEHSEFDNIIQSFEKELEEI 1309
                                           :||      |||....|
  Rat   857 -------------------------------QPS------ENEDKSPH----------------- 867

  Fly  1310 KRSTTSLERKLSNLSEPSPAADEATKAIMEHIAIITGASERSAADEVVLPLNPYDSYDLSSVPRR 1374
                          |.|..|.:|
  Rat   868 --------------SNPDTAGEE------------------------------------------ 876

  Fly  1375 SQSVSAAAQRQSVKLKRRSLEKQRKIDEDFSISNEIRKICDQIHAPFVAMEAMAVAATSASQAQP 1439
                                      ||:
  Rat   877 --------------------------DEE------------------------------------ 879

  Fly  1440 NQSPFLRRKVDPFTVQFDRFKRLSLIERVEEVPEEEKPISTLRIESEKMPRKFLHGPDQLRLDSL 1504
                                             |.|.|:                ||....|..|
  Rat   880 ---------------------------------EPEMPV----------------GPRPRPLSEL 895

  Fly  1505 SLKSTNSYENLLIQKQKLGMATPPAVPATPPTSLKSSIEPPTLAQISSLKTTPPLAALTEHQQHF 1569
            .||.
  Rat   896 HLKE------------------------------------------------------------- 899

  Fly  1570 HATSIQAAPTPAHTHAHSQAHAHSMAGQRRRMEHPQSTLDKAASFQSARTESHSSGAADASSALA 1634
                 :|.|.|
  Rat   900 -----KAVPMP------------------------------------------------------ 905

  Fly  1635 LAMAQKTEQSQSTAPDATQKPSAFTRLTEKPWHCLVSYVDDLTVGGRRNSQGAYNDPMTFPSYGA 1699
                               :.|||...:
  Rat   906 -------------------EASAFFIFS------------------------------------- 914

  Fly  1700 TKAAKVPDDCFPQKCYDHFYFRCPWFMSCMDTQSAKHWTRVRTAVLTVVDTPAFEWFVLVLIFAS 1764
                  |::.|..:|:                              .:|:...|...:|..|..|
  Rat   915 ------PNNRFRLQCH------------------------------RIVNDTIFTNLILFFILLS 943

  Fly  1765 SITLCFEDINLDKNKTLKRVLYWINFSFCLIFVVEMILKWLALGF----SKYFTSFWTILDFIIV 1825
            ||:|..|| .:........:|::.:..|..||.:|:.||..|.|.    ..:..:::.|||.::|
  Rat   944 SISLAAED-PVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVV 1007

  Fly  1826 FVSVFSLLIEENENLKVLRSLRTLRALRPLRAISRWQGMRIVVNALMYAIPSIFNVLLVCLVFWL 1890
            .||:.|..|:.:. :.|::.||.||.|||||||:|.:|::.||..:..||.:|.|:::|..:...
  Rat  1008 SVSLISFGIQSSA-INVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQF 1071

  Fly  1891 IFSIMGVQFFGGKFFKCVNEMGELLPITEVNDKWDCIE-----------QNYTWINSKITFDHVG 1944
            :|:.:|||.|.||.:.|.:...:    ||...|.:.|.           |..:|.|||..||:|.
  Rat  1072 MFACIGVQLFKGKLYTCSDSSKQ----TEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVL 1132

  Fly  1945 MGYLALLQVATFEGWMEVMADAVDARGVDLQPQREANLYAY-----IYFVIFIVCGSFFTLNLFI 2004
            ...:||..|:|||||.|::..::|:...|..|     :|.|     |:|:|:|:..:||.:|:|:
  Rat  1133 AAMMALFTVSTFEGWPELLYRSIDSHTEDKGP-----IYNYRVEISIFFIIYIIIIAFFMMNIFV 1192

  Fly  2005 GVIIDNFNMLKKKYEGGVLEMFLTESQKHYYTAMKKLGRKKPQKVIKRPI---NHFLAMFYDLSN 2066
            |.:|..|.      |.|..|....|..|:....::...:.:|   ::|.|   .|...::| :.|
  Rat  1193 GFVIVTFQ------EQGEQEYKNCELDKNQRQCVEYALKARP---LRRYIPKNQHQYKVWY-VVN 1247

  Fly  2067 SRRFEIAIFVLIFLNMLTMGIEHYDQPHAVFFILEVSNAFFTTVFGLEAIVKIVGLR-YHYFTVP 2130
            |..||..:||||.||.:.:.::||.|.......:.:.|..||.:|.:|.|:|::..: .|||...
  Rat  1248 STYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMILKLIAFKPKHYFCDA 1312

  Fly  2131 WNVFDFLLVLASIFGILMEDI--MIDLPISPTL---------LRVVRVFRIGRILRLIKAAKGIR 2184
            ||.||.|:|:.||..|.:.::  ......||::         :...|:||:.|:::|:...:|||
  Rat  1313 WNTFDALIVVGSIVDIAITEVHPAEHTQCSPSMSAEENSRISITFFRLFRVMRLVKLLSRGEGIR 1377

  Fly  2185 KLLFALVVSLPALFNIGALLGLITFIYAILGMSLFGNVKLQGALDDMV------NFQTFGRSMQL 2243
            .||:..:.|..||..:..|:.::.||||::||.:||.:    ||:|..      |||||.:::.|
  Rat  1378 TLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKI----ALNDTTEINRNNNFQTFPQAVLL 1438

  Fly  2244 LFRLMTSAGWNDVLESLMIQP-----PDCDPFIHGHTNGN--CGHPLLAITYFTSFIIISYMIVI 2301
            |||..|...|.|::  |...|     |:.:|  ...|.|.  ||.. .|:.||.||.::...::|
  Rat  1439 LFRCATGEAWQDIM--LACMPGKKCAPESEP--SNSTEGETPCGSS-FAVFYFISFYMLCAFLII 1498

  Fly  2302 NMYIAIILENFNQAHQEEEIGIVEDDLEMFYIRWSKYDPHATQFIHFSQLSDFIASLDPPLGISK 2366
            |:::|:|::||:...::..| :....|:.|...|::|||.|...|....:...:..:.||||..|
  Rat  1499 NLFVAVIMDNFDYLTRDWSI-LGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGK 1562

  Fly  2367 --PNNVA---LVSFNLPISKGNKIHCLDILHALVKHVLGHVEETDNFKQLQEQMDVKFKKQFPTR 2426
              |:.||   |||.|:|::....:.....|.|||:..| .::...|.:|..|::....||
  Rat  1563 LCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTAL-RIKTEGNLEQANEELRAIIKK----- 1621

  Fly  2427 KELEIVSSTRIWKRQEK---------------------AAKTIQTGWKEYLRR-KREKERSNSGD 2469
                      ||||...                     |...||    ||.|: |:.||:
  Rat  1622 ----------IWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQ----EYFRKFKKRKEQ----- 1667

  Fly  2470 SATQTSSPGGWQSKLSALNFFHLQVSRRGTACSSRASSRKSSRASDASDLSELAGPWLNLPLMLV 2534
                     |...|.|..|...||...|                    .|.:: ||.:.   ..:
  Rat  1668 ---------GLVGKPSQRNALSLQAGLR--------------------TLHDI-GPEIR---RAI 1699

  Fly  2535 SG----ADEVVKDIKQ------QNDELGKRGSIF---VEAPRASRRRSFYNFFL----------- 2575
            ||    .:|:.|.:|:      ::|...:.|.:|   |...::..|.:|...|.
  Rat  1700 SGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVSYYQSDSRSNFPQTFATQRPLHINKTG 1764

  Fly  2576 ---------RHQDAVDDSLTSPSVHRKTAMNNTTNTTSNSA-----STSGTASSTATAPATGCGP 2626
                     .|:..||.:.| ||.:..|..|...|..:|:|     ..:|.:|:.:|....|
  Rat  1765 NNQADTESPSHEKLVDSTFT-PSSYSSTGSNANINNANNTALGRFPHPAGYSSTVSTVEGHG--- 1825

  Fly  2627 AATSASDSDRHQAVGGGSAPSRKRASSFIRKKPPLERGLSAQSALRVNKNAFVSEASAPEVIVTR 2691
                                            |||      ..|:||.:.|:...:....
  Rat  1826 --------------------------------PPL------SPAVRVQEAAWKLSSKRCH----- 1847

  Fly  2692 PSPEQQTHPHSLSLRPDNATLVHVLVHRESEEYKEEDE--SSPSVSGNGNGFGVGLDMLSKQPPP 2754
             |.|.|....|..:.||..        |.|....||.|  |.||:        :..|:||.|..
  Rat  1848 -SRESQGATVSQDMFPDET--------RSSVRLSEEVEYCSEPSL--------LSTDILSYQDD- 1894

  Fly  2755 QIRITTGSVESSMDTCAMPTVQIMVDSPKDPPRGDFSSAPIDDVGAPIDVNVQGDTSQVFYDYNP 2819
                     |:...||.....:.:..|||   |....||.:.              .:..:....
  Rat  1895 ---------ENRQLTCLEEDKREIQPSPK---RSFLRSASLG--------------RRASFHLEC 1933

  Fly  2820 EKATDDQGNGQDETAQFESLP---------------------------DRQR-XPPV-----NTP 2851
            .|...|||.   :.:|..:||                           .|.| .|||     ..|
  Rat  1934 LKRQKDQGG---DISQKTALPLHLVHHQALAVAGLSPLLQRSHSPSTFPRPRPTPPVTPGSRGRP 1995

  Fly  2852 LRDSEKRSSRGSRSSSSSTSSIRS--CSS-SSSSSAASAAAPSARR 2894
            |:........|:.||....||..|  ||| |..::|.|..:..|||
  Rat  1996 LQPIPTLRLEGAESSEKLNSSFPSIHCSSWSEETTACSGGSSMARR 2041

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
NaCP60E	NP_001261172.1	Ion_trans	152..371	CDD:278921	80/241 (33%)
		Ion_trans	705..902	CDD:278921	63/208 (30%)
		Ion_trans	1768..2019	CDD:278921	86/270 (32%)
		Na_channel_gate	2009..2063	CDD:240441	10/56 (18%)
		Ion_trans	2085..2320	CDD:278921	80/259 (31%)
		GPHH	2331..2386	CDD:293510	20/59 (34%)
Cacna1c	NP_036649.2	Calmodulin-binding. /evidence=ECO:0000250\|UniProtKB:Q13936	77..98
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	104..128		6/33 (18%)
		I	141..438		105/375 (28%)
		Ion_trans	171..446	CDD:278921	97/353 (27%)
		TrbL	<232..>321	CDD:294888	23/88 (26%)
		Selectivity filter of repeat I. /evidence=ECO:0000250\|UniProtKB:P07293	391..394		1/2 (50%)
		AID/alpha-interaction domain, mediates interaction with the beta subunit. /evidence=ECO:0000269\|PubMed:15170217	458..475		4/16 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	479..511		11/85 (13%)
		II	540..786		76/253 (30%)
		Ion_trans	571..794	CDD:278921	71/228 (31%)
		Selectivity filter of repeat II. /evidence=ECO:0000250\|UniProtKB:P07293	734..737		1/2 (50%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	794..891		42/578 (7%)
		Interaction with STAC2. /evidence=ECO:0000250\|UniProtKB:Q13936	859..906		23/375 (6%)
		Ion_trans	947..1206	CDD:278921	88/275 (32%)
		Dihydropyridine binding. /evidence=ECO:0000250\|UniProtKB:P07293	1119..1208		35/99 (35%)
		Selectivity filter of repeat III. /evidence=ECO:0000250\|UniProtKB:P07293	1143..1146		2/2 (100%)
		IV	1236..1509		90/282 (32%)
		Ion_trans	1266..1517	CDD:278921	80/259 (31%)
		Selectivity filter of repeat IV. /evidence=ECO:0000250\|UniProtKB:P07293	1444..1447		1/2 (50%)
		Dihydropyridine binding. /evidence=ECO:0000250\|UniProtKB:P07293	1460..1528		19/71 (27%)
		Phenylalkylamine binding. /evidence=ECO:0000250\|UniProtKB:P07293	1474..1516		13/42 (31%)
		GPHH	1525..1588	CDD:293510	20/62 (32%)
		Ca_chan_IQ	1590..1661	CDD:285917	20/90 (22%)
		Calmodulin-binding. /evidence=ECO:0000250\|UniProtKB:Q13936	1641..1674		11/50 (22%)
		Important for interaction with STAC1, STAC2 and STAC3. /evidence=ECO:0000250\|UniProtKB:P15381	1641..1668		9/44 (20%)
		Calmodulin-binding IQ region. /evidence=ECO:0000250\|UniProtKB:Q13936	1647..1667		8/23 (35%)
		Important for localization in at the junctional membrane. /evidence=ECO:0000250\|UniProtKB:P15381	1681..1700		6/42 (14%)
		CAC1F_C	1684..2117	CDD:293490	94/476 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1762..1794		7/32 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1971..1999		7/27 (26%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	2	1.910