Protein Alignment NaCP60E and Scn8a

DIOPT Version :10

• Sequence 1: NP_001261172.1
• Gene: NaCP60E / 37981
• FlyBaseID: FBgn0085434
• Length: 2896
• Species: Drosophila melanogaster
• Sequence 2: XP_017172001.1
• Gene: Scn8a / 20273
• MGIID: 103169
• Length: 1988
• Species: Mus musculus
• Alignment Length: 2676
• Identity: 821/2676 - (30%)
• Similarity: 1170/2676 - (43%)
• Gaps: 899/2676 - (33%)

  Fly    45 PRTKASVEDGDVLPRKFEPFPEHMYGKPLEEIDT-FIYEETFCVVSKRFRKNYIHRFTGTKSLFL 108
            |:..:.:|.|..||..:...|:.:...|||:.|. ::.::||.|::   |...:.||:.|.:|::
Mouse    53 PKPNSDLEAGKSLPFIYGDIPQGLVAVPLEDFDPYYLTQKTFVVLN---RGKTLFRFSATPALYI 114

  Fly   109 FYPWSPARRVCVYIATNQFFDYCVMATILFNCIFLAMT---ETVEEAEYIFLAIYSIEMVIKIIA 170
            ..|::..||:.:.|..:..|...:|.|||.||:|:..:   |..:..||.|..||:.|.::||||
Mouse   115 LSPFNLIRRIAIKILIHSVFSMIIMCTILTNCVFMTFSNPPEWSKNVEYTFTGIYTFESLVKIIA 179

  Fly   171 KGFLLNKYTYLRNPWNWLDFVVITSGYATIGMEVGNLAGLRTFRVLRALKTVSIMPGLKTIINAL 235
            :||.::.:|:||:|||||||.||...|.|..:::||::.||||||||||||:|::||||||:.||
Mouse   180 RGFCIDGFTFLRDPWNWLDFSVIMMAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGAL 244

  Fly   236 LHSFRQLAEVMTLTIFCLMVFALFALQVYMGELRNKCVRQVPTDWT---NVSHT-------DWQI 290
            :.|.::|::||.||:|||.||||..||::||.||||||     .|.   |.|:.       ||:.
Mouse   245 IQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCV-----VWPINFNESYLENGTRGFDWEE 304

  Fly   291 WVNDTDNWLYDEDEL-PVLCGNLTGARHCPFEYVCLCVGENPNHGYTNFDNFMWSMLTTFQLITL 354
            ::|:..|:......| |:||||.:.|..||..:.|:..|.|||:|||:||.|.|:.|..|:|:|.
Mouse   305 YINNKTNFYMVPGMLEPLLCGNSSDAGQCPEGFQCMKAGRNPNYGYTSFDTFSWAFLALFRLMTQ 369

  Fly   355 DYWENVYNMVLATCGPMSVSFFTVVVFFGSFYLINLMLAVVALSYEEEAEITNEERKKDLLDHRD 419
            |||||:|.:.|...|...:.||.:|:|.|||||:||:|||||::|||:.:.|.||.:      :.
Mouse   370 DYWENLYQLTLRAAGKTYMIFFVLVIFVGSFYLVNLILAVVAMAYEEQNQATLEEAE------QK 428

  Fly   420 DSTFSFDPSVLNVKKLNKNNKKKIDSRKGVLLASYSKKKTRRKKTKGG-------KEGGTNGNGN 477
            ::.|         |.:.:..||:.:..:...:|           |..|       :|.|.:|.|:
Mouse   429 EAEF---------KAMLEQLKKQQEEAQAAAMA-----------TSAGTVSEDAIEEEGEDGVGS 473

  Fly   478 GSNGDDNKSHSATPSPGPSPRHSATERPSALTMQAQKQYQQMEQQ---HKLAKSGS-GGSNNPMA 538
            ..:..:....|:         .||.||.:....:.||:..:.|::   .|:.||.| .|......
Mouse   474 PRSSSELSKLSS---------KSAKERRNRRKKRKQKELSEGEEKGDPEKVFKSESEDGMRRKAF 529

  Fly   539 PTPKGRIS-----FQDSGMGVKNPNMLYPSDYKGQLIANSGQPSSNSSGVNRESSQDDSGVVDDH 598
            ..|..||.     ...|.:.:.....|...:.|..:.:..|.......|...|.:.|:...|::.
Mouse   530 RLPDNRIGRKFSIMNQSLLSIPGSPFLSRHNSKSSIFSFRGPGRFRDPGSENEFADDEHSTVEES 594

  Fly   599 EEQDTTNDMGHVSTVELALSPREVR------------------------------LIKCNGNIAR 633
            |        |...::.:.:..||.|                              .:.|||.::.
Mouse   595 E--------GRRDSLFIPIRARERRSSYSGYSGYSQCSRSSRIFPSLRRSVKRNSTVDCNGVVSL 651

  Fly   634 I-KNHNVYALHQEF----------SSEV-----------------------------------VV 652
            | ...::..|..|.          ::||                                   .:
Mouse   652 IGPGSHIGRLLPEVKIDKAATDSATTEVEIKKKGPGSLLVSMEQLASYGRKDRINSIMSVVTNTL 716

  Fly   653 IDDLPD--RNCDRCVH--------W-CTDYESWLQFQNCLYKVVRDPLFELAITLCIVLNTAFLA 706
            :::|.:  |.|..|.:        | |..|  |::.:..:..:|.||..:||||:||||||.|:|
Mouse   717 VEELEESQRKCPPCWYKFANTFLIWECHPY--WIKLKEIVNLIVMDPFVDLAITICIVLNTLFMA 779

  Fly   707 MEHHGMSESFRNALDVGNKVFTSIFTFECIVKLMALSK-DFFLCGWNIFDLLIVTASLLDIIFEL 770
            ||||.|:..|.:.|.|||.|||.|||.|..:||:|:.. .:|..||||||..||:.||:::....
Mouse   780 MEHHPMTPQFEHVLAVGNLVFTGIFTAEMFLKLIAMDPYYYFQEGWNIFDGFIVSLSLMELGLAD 844

  Fly   771 VDGLSVLRGLRLLRVLKLAQSWTTMKVLLSIIISTIGALGNLTLILVIVIYIFAVIGMQLFSKDY 835
            |:||||||..|||||.|||:||.|:.:|:.||.:::||||||||:|.|:::||||:|||||.|.|
Mouse   845 VEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSY 909

  Fly   836 TP--EKFDPD-PVPRWNFNDFFHSFMMIFRILCGEWIEPLWDCMRAEEEQGASTCFAIFLPTLVM 897
            ..  .|...: .:|||:.|||||||:::||:|||||||.:||||   |..|.:.|..:|:..:|:
Mouse   910 KECVCKISQECKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCM---EVAGQAMCLIVFMMVMVI 971

  Fly   898 GNFMVLNLFLALLLNSFNSEELKSKKEEVGEESKLARSIERVRDLIRKKRQERKDRKERKFAEKF 962
            ||.:||||||||||:||:::.|.:..:: ||.:.|..|:.|:
Mouse   972 GNLVVLNLFLALLLSSFSADNLAATDDD-GEMNNLQISVIRI----------------------- 1012

  Fly   963 QQIVLDAQQAHAQTLSHQAAVGLERGDKPGVL-AETKFHRLSYQESMNRPVSGSDFGFQIPLHDG 1026
                                       |.||. |:.|.|..
Mouse  1013 ---------------------------KKGVAWAKVKVHAF------------------------ 1026

  Fly  1027 LHTIVDGLEYDDTGDLPEQIQLQAHPLPPTSDSMPP---TYE---SAMMATTG------GSFSSV 1079
                                 :|||.....:|.:.|   .||   :.:...||      |.|..
Mouse  1027 ---------------------MQAHFKQREADEVKPLDELYEKKANCIANHTGVDIHRNGDFQK- 1069

  Fly  1080 NGNGTCQNLTPFVQAERRLQHQISSGVSTQQYDSREEATYTESIELLGQYNSTDTDPYANDQRSG 1144
            |||||                                                         .||
Mouse  1070 NGNGT---------------------------------------------------------TSG 1077

  Fly  1145 CGSFNRGDSLQDNSSRRYGSEEHDEAFLKYQKSLLTRSPSYRKSLDRLSQSSGQSQRSLLKSEEA 1209
            .||          |..:|..:|...:|:. ..:|..|.|
Mouse  1078 IGS----------SVEKYIIDEDHMSFIN-NPNLTVRVP-------------------------- 1105

  Fly  1210 EMRRHSSGQSLNSMSIEQDELLSQQGNLREELLNCDQKELFQFLQEEEELQKGTKLRRISNVMRS 1274
                                                                             
Mouse  1106 ----------------------------------------------------------------- 1105

  Fly  1275 RRPSSQMGQPENETMVEHSEFDNIIQSFEKELEEIKRSTTSLERKLSNLSEPSPAADEATKAIME 1339
                         ..|..|:|:|:      ..|::           |:.|:|..:.|
Mouse  1106 -------------IAVGESDFENL------NTEDV-----------SSESDPEGSKD-------- 1132

  Fly  1340 HIAIITGASERSAADEVVLPLNPYDSYDLSSVPRRSQSVSAAAQRQSVKLKRRSLEKQRKIDEDF 1404
                                                                       |:|:
Mouse  1133 -----------------------------------------------------------KLDD-- 1136

  Fly  1405 SISNEIRKICDQIHAPFVAMEAMAVAATSASQAQPNQSPFLRRKVDPFTVQFDRFKRLSLIERVE 1469
                                       ||:|:.                      ..:.:...||
Mouse  1137 ---------------------------TSSSEG----------------------STIDIKPEVE 1152

  Fly  1470 EVPEEEKPISTLRIESEKMPRKFLHGPDQLRLDSLSLKSTNSYENLLIQKQKLGMATPPAVPATP 1534
            |||
Mouse  1153 EVP-------------------------------------------------------------- 1155

  Fly  1535 PTSLKSSIEPPTLAQISSLKTTPPLAALTEHQQHFHATSIQAAPTPAHTHAHSQAHAHSMAGQRR 1599
                                                                             
Mouse  1156 ----------------------------------------------------------------- 1155

  Fly  1600 RMEHPQSTLDKAASFQSARTESHSSGAADASSALALAMAQKTEQSQSTAPDATQKPSAFTRLTEK 1664
             :|.|:..||
Mouse  1156 -VEQPEEYLD------------------------------------------------------- 1164

  Fly  1665 PWHCLVSYVDDLTVGGRRNSQGAYNDPMTFPSYGATKAAKVPDDCFPQKCYDHFYFRCPWFMSC- 1728
                                                     ||.||.:.|...  |:|     |
Mouse  1165 -----------------------------------------PDACFTEGCVQR--FKC-----CQ 1181

  Fly  1729 --MDTQSAKHWTRVRTAVLTVVDTPAFEWFVLVLIFASSITLCFEDINLDKNKTLKRVLYWINFS 1791
              ::....|.|..:|.....:|:...||.|::.:|..||..|.||||.:::.||::.:|.:.:..
Mouse  1182 VNIEEGLGKSWWILRKTCFLIVEHNWFETFIIFMILLSSGALAFEDIYIEQRKTIRTILEYADKV 1246

  Fly  1792 FCLIFVVEMILKWLALGFSKYFTSFWTILDFIIVFVSVFSLLIEE--NENLKVLRSLRTLRALRP 1854
            |..||::||:|||.|.||.|:||:.|..|||:||.||:.||:...  ...|..::||||||||||
Mouse  1247 FTYIFILEMLLKWTAYGFVKFFTNAWCWLDFLIVAVSLVSLIANALGYSELGAIKSLRTLRALRP 1311

  Fly  1855 LRAISRWQGMRIVVNALMYAIPSIFNVLLVCLVFWLIFSIMGVQFFGGKFFKCVNEMGEL-LPIT 1918
            |||:||::|||:|||||:.|||||.|||||||:||||||||||..|.||:..|.||..|: ..|.
Mouse  1312 LRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYHYCFNETSEIRFEID 1376

  Fly  1919 EVNDKWDC---IEQNYT---WINSKITFDHVGMGYLALLQVATFEGWMEVMADAVDARGVDLQPQ 1977
            |||:|.||   :|.|.|   |.|.||.||:||.|||||||||||:|||::|..|||:|..|.||.
Mouse  1377 EVNNKTDCEKLMEGNNTEIRWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRKPDEQPD 1441

  Fly  1978 REANLYAYIYFVIFIVCGSFFTLNLFIGVIIDNFNMLKKKYEGGVLEMFLTESQKHYYTAMKKLG 2042
            .|.|:|.||||||||:.||||||||||||||||||..|||:.|  .::|:||.||.||.||||||
Mouse  1442 YEGNIYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGG--QDIFMTEEQKKYYNAMKKLG 1504

  Fly  2043 RKKPQKVIKRPINHFLAMFYDLSNSRRFEIAIFVLIFLNMLTMGIEHYDQPHAVFFILEVSNAFF 2107
            .|||||.|.||:|....:.:|....:.|:|.|.:||.|||:||.:|...|...:..||...|..|
Mouse  1505 SKKPQKPIPRPLNKIQGIVFDFVTQQAFDIVIMMLICLNMVTMMVETDTQSKQMENILYWINLVF 1569

  Fly  2108 TTVFGLEAIVKIVGLRYHYFTVPWNVFDFLLVLASIFGILMEDIMIDLPISPTLLRVVRVFRIGR 2172
            ...|..|.::|:..||::|||:.||:|||::|:.||.|:.:.||:....:||||.||:|:.||||
Mouse  1570 VIFFTCECVLKMFALRHYYFTIGWNIFDFVVVILSIVGMFLADIIEKYFVSPTLFRVIRLARIGR 1634

  Fly  2173 ILRLIKAAKGIRKLLFALVVSLPALFNIGALLGLITFIYAILGMSLFGNVKLQGALDDMVNFQTF 2237
            ||||||.|||||.|||||::|||||||||.||.|:.||::|.|||.|..||.:..:|||.||:||
Mouse  1635 ILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYVKHEAGIDDMFNFETF 1699

  Fly  2238 GRSMQLLFRLMTSAGWNDVLESLMIQPPDC--DPFIHGH-TNGNCGHPLLAITYFTSFIIISYMI 2299
            |.||..||::.|||||:.:|..::.:||||  |....|. ..|:||:|.:.|.:|.|:||||::|
Mouse  1700 GNSMICLFQITTSAGWDGLLLPILNRPPDCSLDKEHPGSGFKGDCGNPSVGIFFFVSYIIISFLI 1764

  Fly  2300 VINMYIAIILENFNQAHQEEEIGIVEDDLEMFYIRWSKYDPHATQFIHFSQLSDFIASLDPPLGI 2364
            |:|||||||||||:.|.:|....:.|||.|.||..|.|:||.|||||.:.:|:||..:|:.||.:
Mouse  1765 VVNMYIAIILENFSVATEESADPLSEDDFETFYEIWEKFDPDATQFIEYCKLADFADALEHPLRV 1829

  Fly  2365 SKPNNVALVSFNLPISKGNKIHCLDILHALVKHVLGHVEETDNFKQLQEQMDVKFKKQFPTRKEL 2429
            .|||.:.|::.:||:..|::|||||||.|..|.|||...|.|   .|::||:.:|....|::...
Mouse  1830 PKPNTIELIAMDLPMVSGDRIHCLDILFAFTKRVLGDSGELD---ILRQQMEERFVASNPSKVSY 1891

  Fly  2430 EIVSSTRIWKRQEKAAKTIQTGWKEYLRRKREKERSNSGDSATQTSSPGGWQSKLSALNFFHLQV 2494
            |.:::|...|::|.:|..:|..::.:|
Mouse  1892 EPITTTLRRKQEEVSAVVLQRAYRGHL-------------------------------------- 1918

  Fly  2495 SRRGTACSSRASSRKSSRASDASDLSELAGPWLNLPLMLVSGADEVVK--DIKQQNDELGKRGSI 2557
            :|||..| .:.:|.|........:..|......:||     ..|.|.|  ..|||..|.|:|
Mouse  1919 ARRGFIC-RKITSNKLENGGTHREKKESTPSTASLP-----SYDSVTKPDKEKQQRAEEGRR--- 1974

  Fly  2558 FVEAPRASRRR 2568
                .||.|::
Mouse  1975 ----ERAKRQK 1981

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NaCP60E	NP_001261172.1	Ion_trans	125..371	CDD:459842	121/259 (47%)
		Ion_trans	687..902	CDD:459842	118/218 (54%)
		Ion_trans	1751..2019	CDD:459842	163/276 (59%)
		Na_channel_gate	2009..2063	CDD:240441	30/53 (57%)
		Ion_trans	2067..2320	CDD:459842	133/255 (52%)
		GPHH	2332..2378	CDD:465306	20/45 (44%)
Scn8a	XP_017172001.1	Ion_trans	131..421	CDD:459842	140/294 (48%)
		Na_trans_cytopl	546..710	CDD:463401	21/171 (12%)
		Ion_trans	760..973	CDD:459842	116/215 (54%)
		Na_trans_assoc	999..1200	CDD:461936	72/835 (9%)
		Ion_trans	1205..1481	CDD:459842	161/275 (59%)
		Na_channel_gate	1473..1525	CDD:240441	30/53 (57%)
		Ion_trans	1530..1784	CDD:459842	132/253 (52%)
		IQ	1902..1923	CDD:197470	6/58 (10%)