Protein Alignment NaCP60E and Cacna1b

DIOPT Version :10

• Sequence 1: NP_001261172.1
• Gene: NaCP60E / 37981
• FlyBaseID: FBgn0085434
• Length: 2896
• Species: Drosophila melanogaster
• Sequence 2: XP_006497689.1
• Gene: Cacna1b / 12287
• MGIID: 88296
• Length: 2351
• Species: Mus musculus
• Alignment Length: 2877
• Identity: 635/2877 - (22%)
• Similarity: 1066/2877 - (37%)
• Gaps: 889/2877 - (30%)

  Fly    80 IYEETFCVVSKRFRKNYIHR--------FTGTKSLFLFYPWSPARRVCVYIATNQFFDYCVMATI 136
            :|:::   :::|.|...::.        ||..:|||:|...:..|:....|.....|:|.::|||
Mouse    44 LYKQS---IAQRARTMALYNPIPVKQNCFTVNRSLFVFSEDNVVRKYAKRITEWPPFEYMILATI 105

  Fly   137 LFNCIFLA------------MTETVEEAEYIFLAIYSIEMVIKIIAKGFLLNKYTYLRNPWNWLD 189
            :.|||.||            |:|.:::.|..|:.|:..|..|||||.||:.:|.:||||.||.:|
Mouse   106 IANCIVLALEQHLPDGDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKGSYLRNGWNVMD 170

  Fly   190 FVVITSG-YATIGMEVGNLAGLRTFRVLRALKTVSIMPGLKTIINALLHSFRQLAEVMTLTIFCL 253
            |||:.:| .||.|.:. :|..||..||||.||.||.:|.|:.::.:::.:...|.::..|..|.:
Mouse   171 FVVVLTGILATAGTDF-DLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAI 234

  Fly   254 MVFALFALQVYMGELRNKCVRQVPTDWTNVSHTDWQIWVNDTDNWLYDEDELPVLCGNLTGARHC 318
            ::||:..|:.|||:....|                  :.|.||.  ....:.|  ||....||.|
Mouse   235 LMFAIIGLEFYMGKFHKAC------------------FPNSTDT--EPVGDFP--CGKDPPARQC 277

  Fly   319 PFEYVCLCVGENPNHGYTNFDNFMWSMLTTFQLITLDYWENV-YNMVLATCGPMSVSFFTVVVFF 382
            ..:..|......||.|.|||||.::::||.||.||::.|.:: ||...|.....:..:|..::..
Mouse   278 DGDTECREYWPGPNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPLIII 342

  Fly   383 GSFYLINLMLAVVALSYEEEAEITNEERKKDLLDHRDDSTFSFDPSVLNVKKLNKNNKKKIDSRK 447
            |||:::||:|.|::..:.:|.|  ..|.::..|..|......        ::|| ...:.|...:
Mouse   343 GSFFMLNLVLGVLSGEFAKERE--RVENRRAFLKLRRQQQIE--------RELN-GYLEWIFKAE 396

  Fly   448 GVLLASYSKKKTRRKKTKGGKEGGTNGNGNGSNGDDNKSHSATPSPGPSPRHSATERPSALTMQA 512
            .|:||.                            :|..:...:|......| :||::
Mouse   397 EVMLAE----------------------------EDKNAEEKSPLDAVLKR-AATKK-------- 424

  Fly   513 QKQYQQMEQQHKLAKSGSGGSNNPMAPTPKGRISFQDSGMGVKNPNMLYPSDYKGQLIANSGQP- 576
                                |.|.:....:|...|.|                    :...|.|
Mouse   425 --------------------SRNDLIHAEEGEDRFVD--------------------LCAVGSPF 449

  Fly   577 --SSNSSGVNRESSQDDSGVVDDHEEQDTTNDMGHVSTVELALSPREVRLIKCNGNIARIKNHNV 639
              :|..||....||
Mouse   450 ARASLKSGKTESSS--------------------------------------------------- 463

  Fly   640 YALHQEFSSEVVVIDDLPDRNCDRCVHWCTDYESWLQFQNCLYKVVRDPLFELAITLCIVLNTAF 704
            |...:|                             ..|:..:.::|:...|...:...:.|||..
Mouse   464 YFRRKE-----------------------------KMFRFFIRRMVKAQSFYWVVLCVVALNTLC 499

  Fly   705 LAMEHHGMSESFRNALDVGNKVFTSIFTFECIVKLMALS-KDFFLCGWNIFDLLIVTASLLDIIF 768
            :||.|:...:....||.....||..:|..|..:|:..|. :.:|...:|.||..::..|:.::::
Mouse   500 VAMVHYNQPQRLTTALYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSFNCFDFGVIVGSIFEVVW 564

  Fly   769 ELVD-----GLSVLRGLRLLRVLKLAQSWTTMKVLLSIIISTIGALGNLTLILVIVIYIFAVIGM 828
            ..:.     |:||||.|||||:.|:.:.|.:::.|:..:::::.::.:|..:|.:.|.:||::||
Mouse   565 AAIKPGTSFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGM 629

  Fly   829 QLFSKDYTPEKFDPDPVPRWNFNDFFHSFMMIFRILCGE-WIEPLWDCMRAE--EEQGASTCFAI 890
            |||...:..:    |..|..||:.|..:.:.:|:||.|| |...::..:.::  ..:|..:.|..
Mouse   630 QLFGGQFNFQ----DETPTTNFDTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYF 690

  Fly   891 FLPTLVMGNFMVLNLFLALLL-NSFNSEELKSKKEEVGEESKLARSIERVRDLIRKKRQERKDRK 954
            .:.|| .||:.:||:|||:.: |..|::||...:||:.|.:....::::.::
Mouse   691 IVLTL-FGNYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKE------------- 741

  Fly   955 ERKFAEKFQQIVLDAQQAHAQTLSHQAAVGLERGDKPGVLAETKFHRLSYQESMNRPVSGSDFGF 1019
                       |.:.....|..:|..|.|...||                      .||.|
Mouse   742 -----------VAEVSPMSAANISIAAFVKQTRG----------------------TVSRS---- 769

  Fly  1020 QIPLHDGLHTIVDGLEYDDTGDLPEQIQLQAHPLPPTSDSMPPTYESAMMATTGGSFSSVNGNGT 1084
                                                                  .|.||||
Mouse   770 ------------------------------------------------------SSVSSVN---- 776

  Fly  1085 CQNLTPFVQ---AERRLQHQISSGVSTQQYDSREEATYTESIELLGQYNSTDTDPYANDQRSGCG 1146
                :|..|   ..|.:..|.:|.:..|...:..||.|:|            .||   ::|
Mouse   777 ----SPRQQNSAKARSVWEQRASQLRLQNLRASCEALYSE------------MDP---EER---- 818

  Fly  1147 SFNRGDSLQDNSSRRYGSEEHDEAFLKYQKSLLTRSPSYRKSLDRLSQSSGQSQRSLLKSEEAEM 1211
                         .||.|..|..             |..:..:||
Mouse   819 -------------LRYASTRHVR-------------PDMKTHMDR-------------------- 837

  Fly  1212 RRHSSGQSLNSMSIEQDELLSQQGNLREELLNCDQKELFQFLQEEEELQKGTKLRRISNVMRSRR 1276
                             .|:.:.|  |:.|                                 |.
Mouse   838 -----------------PLVVEPG--RDGL---------------------------------RG 850

  Fly  1277 PSSQMGQPENETMVEHSEFDNIIQSFEKELEEIKRSTTSLERKLSNLSEPSPAADEATKAIMEHI 1341
            |.....:||.....|.::..             :|.....:|..::.:.|:....:.|::..
Mouse   851 PVGSKSKPEGTEATESADLP-------------RRHHRHRDRDKTSATAPAGGEQDRTESTE--- 899

  Fly  1342 AIITGASERSAADEVVLPLNPYDSYDLSSVPRRSQSVSAAAQRQSVKLKRRSLEKQRKIDEDFSI 1406
               |||.|..|.                  ||||.|.........|:.:|.....:|...|: :.
Mouse   900 ---TGAREERAR------------------PRRSHSKETPGADTQVRCERSRRHHRRGSPEE-AT 942

  Fly  1407 SNEIRKICDQIHAPFVAMEAMAVA--------------ATSASQAQPNQSPF----LRRKVDPFT 1453
            ..|.|:.....||...:.|..|..              .|...:|:.|:.|.    .|.||.|
Mouse   943 EREPRRHRAHRHAQDSSKEGTAPVLVPKGERRARHRGPRTGPREAENNEEPTRRHRARHKVPP-- 1005

  Fly  1454 VQFDRFKRLSLIERVEEVPEEEKPISTLRIESEKMPRKFLHGPDQLRLDSLSLKSTNSYENLLIQ 1518
                   .|...||  |..|:|    :..:|.:|..|.  |.|.:...|..::..|
Mouse  1006 -------TLQPPER--EAAEKE----SNAVEGDKETRN--HQPKEPHCDLEAIAVT--------- 1046

  Fly  1519 KQKLGMATPPAVPATPPTSLKSSIEPPTLAQISSLKTTPPLAALTEHQQHFHATSIQAAPTPAHT 1583
                |:.   .:...|.|.|:...|.|..|               ::|:  :.|.:.:.|:...|
Mouse  1047 ----GVG---PLHMLPSTCLQKVDEQPEDA---------------DNQR--NVTRMGSQPSDPST 1087

  Fly  1584 HAHSQAHAHSMAGQRRRMEHPQSTLDKAASFQSARTESHSSGAADASSALALAMAQKTEQSQSTA 1648
            ..|.........|:...:  |...::         .|..:.|..:|.:
Mouse  1088 TVHVPVTLTGPPGETPVV--PSGNMN---------LEGQAEGKKEAEA----------------- 1124

  Fly  1649 PDATQKPSAFTRLTEKPWHCLVSYVDDLTVGGRRNSQGAYNDPMTFPSYGATKAAKVPDDCFPQK 1713
                                     ||:...|          |.....|.:
Mouse  1125 -------------------------DDVLRRG----------PRPIVPYSS-------------- 1140

  Fly  1714 CYDHFYFRCPWFMSCMDTQSAKHWTRVRTAVLTVVDTPAFEWFVLVLIFASSITLCFED-INLD- 1776
                        |.|:...:.     :|.....:|....||..:||:|..|||.|..|| :..|
Mouse  1141 ------------MFCLSPTNL-----LRRFCHYIVTMRYFEMVILVVIALSSIALAAEDPVRTDS 1188

  Fly  1777 -KNKTLKRVLYWINFSFCLIFVVEMILKWLALGF----SKYFTSFWTILDFIIV----FVSVFSL 1832
             :|..||    ::::.|..:|..||::|.:.||.    ..||...|.|||||:|    ....||.
Mouse  1189 FRNNALK----YMDYIFTGVFTFEMVIKMIDLGLLLHPGAYFRDLWNILDFIVVSGALVAFAFSS 1249

  Fly  1833 LI--EENENLKVLRSLRTLRALRPLRAISRWQGMRIVVNALMYAIPSIFNVLLVCLVFWLIFSIM 1895
            .:  .:.:::..::|||.||.||||:.|.|...::.|.:.::.::.::.|:|:|.::|..||:::
Mouse  1250 FMGGSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVI 1314

  Fly  1896 GVQFFGGKFFKCVNEMGELLPITEVNDKWDC-----------IE-QNYTWINSKITFDHVGMGYL 1948
            .||.|.||||.|.:|..||        :.||           :| |...|......:|:|....|
Mouse  1315 AVQLFKGKFFYCTDESKEL--------ERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALL 1371

  Fly  1949 ALLQVATFEGWMEVMADAVDARGVDLQP----QREANLYAYIYFVIFIVCGSFFTLNLFIGVIID 2009
            .|..|:|.|||..|:..:|||...:..|    :.|.:::..:|||:|    .||.:|:|:.:||.
Mouse  1372 TLFTVSTGEGWPMVLKHSVDATYEEQGPSPGFRMELSIFYVVYFVVF----PFFFVNIFVALIII 1432

  Fly  2010 NFNMLKKKYEGGVLEMFLTESQK---HYYTAMKKLGRKKPQKVIKRPINHFLAMFYDLSNSRRFE 2071
            .|   :::.:..:.|..|.::::   .:..:.|.|.|..||..     ..|....:....|..||
Mouse  1433 TF---QEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNK-----QSFQYKTWTFVVSPPFE 1489

  Fly  2072 IAIFVLIFLNMLTMGIEHYDQPHAVFFILEVSNAFFTTVFGLEAIVKIVGLR-YHYFTVPWNVFD 2135
            ..|..:|.||.:.:.::.||.|:....:|:..|..||::|.:|.|:||:... .:||...|||||
Mouse  1490 YFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFTSMFSMECILKIIAFGVLNYFRDAWNVFD 1554

  Fly  2136 FLLVLASIFGILMEDI-----MIDLPISPTLLRVVRVFRIGRILRLIKAAKGIRKLLFALVVSLP 2195
            |:.||.||..||:.:|     .|:|..       :|:||..|:::|::....||.||:..|.|..
Mouse  1555 FVTVLGSITDILVTEIAETNNFINLSF-------LRLFRAARLIKLLRQGYTIRILLWTFVQSFK 1612

  Fly  2196 ALFNIGALLGLITFIYAILGMSLFGNVKLQGALDDMV------NFQTFGRSMQLLFRLMTSAGWN 2254
            ||..:..|:.::.|||||:||.:|||:    ||||..      ||:||.:::.||||..|...|:
Mouse  1613 ALPYVCLLIAMLFFIYAIIGMQVFGNI----ALDDDTSINRHNNFRTFLQALMLLFRSATGEAWH 1673

  Fly  2255 DVLESLMIQPPDCDPFIHGHTNGN-CGHPLLAITYFTSFIIISYMIVINMYIAIILENFNQAHQE 2318
            :::.|.:.... |||    |.|.: ||.. .|..||.|||.:...:::|:::|:|::||....::
Mouse  1674 EIMLSCLGNRA-CDP----HANASECGSD-FAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRD 1732

  Fly  2319 EEIGIVEDDLEMFYIRWSKYDPHATQFIHFSQLSDFIASLDPPLGISK--PNNVA---LVSFNLP 2378
            ..| :....|:.|...|::|||.|...|.::.:.:.:..:.||||:.|  |..||   ||..|:|
Mouse  1733 SSI-LGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLGLGKKCPARVAYKRLVRMNMP 1796

  Fly  2379 ISKGN-KIHCLDILHALVKHVLGHVEETDNFKQLQEQMDVKFKKQFPT------RKELEIVSSTR 2436
            ||..: .:|....|.||::..|.........|  |.|.|.:.:|:..:      :|.|:::....
Mouse  1797 ISNEDMTVHFTSTLMALIRTALEIKLAPAGTK--QHQCDAELRKEISSVWANLPQKTLDLLVPPH 1859

  Fly  2437 IWKRQEKAAKTIQTG---WKEYLRRKREKERSNSGDSATQTSSPGGWQSKLSALNFFH-LQVSRR 2497
              |..|.....:...   :..|.:.|..:::::        .:||| .|::..::.|| |:.:..
Mouse  1860 --KPDEMTVGKVYAALMIFDFYKQNKTTRDQTH--------QAPGG-LSQMGPVSLFHPLKATLE 1913

  Fly  2498 GTACSSRASSRKSSRASDASDLSELAGPWLNLPLMLVSGADEVVKDIKQQNDELGKRGSIFVEAP 2562
            .|..:....:|...|...|:.||.             .||        .|..|.|.:.|:.....
Mouse  1914 QTQPAVLRGARVFLRQKSATSLSN-------------GGA--------IQTQESGIKESLSWGTQ 1957

  Fly  2563 RASRRRSFYNFFLRHQDAVDDSLTSPSVHRKTAMNNTTNTTSNSASTSGT-ASSTATAPATGCGP 2626
            |.             |||         ::...|.....::.......||| |........|..||
Mouse  1958 RT-------------QDA---------LYEARAPLERGHSAEIPVGQSGTLAVDVQMQNMTLRGP 2000

  Fly  2627 AATSASDSDRHQAV-GGGSAPSRKRASSFIRKKP---PLERGLSAQSALRVNKNAFVSEASAPEV 2687
                  |.:....: ..|.|.|..|.::..:..|   |::|.:|. .|.|.:.....|      .
Mouse  2001 ------DGEPQPGLESQGRAASMPRLAAETQPAPNASPMKRSIST-LAPRPHGTQLCS------T 2052

  Fly  2688 IVTRPSPEQQTHPH-------------------SLSLRPDNATLVHV---LVH----------RE 2720
            ::.||.|.|.:|.|                   |||:.|:.|.....   |.|          |:
Mouse  2053 VLDRPPPSQASHHHHHRCHRRRDKKQRSLEKGPSLSVDPEGAPSTAAGPGLPHGEGSTACRRDRK 2117

  Fly  2721 SEEYKEEDESSPSVSGNGNGFGVGLDMLSKQPPPQIRITTGSVESSMDTCAMPTVQIMVDSPKDP 2785
            .|..:.::...||.|.:........|....:.|||:..:..|..:|      ||..:   .|...
Mouse  2118 QERGRSQERRQPSSSSSEKQRFYSCDRFGSREPPQLMPSLSSHPTS------PTAAL---EPAPH 2173

  Fly  2786 PRGDFSSAPIDDVGAPI 2802
            |:|   |..::  |:|:
Mouse  2174 PQG---SGSVN--GSPL 2185

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NaCP60E	NP_001261172.1	Ion_trans	125..371	CDD:459842	89/259 (34%)
		Ion_trans	687..902	CDD:459842	60/223 (27%)
		Ion_trans	1751..2019	CDD:459842	93/296 (31%)
		Na_channel_gate	2009..2063	CDD:240441	9/56 (16%)
		Ion_trans	2067..2320	CDD:459842	93/265 (35%)
		GPHH	2332..2378	CDD:465306	17/50 (34%)
Cacna1b	XP_006497689.1	Ion_trans	94..367	CDD:459842	99/297 (33%)
		Ion_trans	482..721	CDD:459842	69/243 (28%)
		Granin	<936..>1041	CDD:279595	27/122 (22%)
		Ion_trans	1161..1395	CDD:459842	80/245 (33%)
		Ion_trans	1485..1734	CDD:459842	93/265 (35%)
		GPHH	1743..1796	CDD:465306	18/52 (35%)
		Ca_chan_IQ	1807..1884	CDD:462591	14/80 (18%)
		PHA03307	1959..>2326	CDD:223039	55/276 (20%)