DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Usp15-31 and Usp4

DIOPT Version :9

Sequence 1:	NP_611959.2	Gene:	Usp15-31 / 37954	FlyBaseID:	FBgn0050421	Length:	1272	Species:	Drosophila melanogaster
Sequence 2:	NP_001128484.1	Gene:	Usp4 / 290864	RGDID:	1587387	Length:	961	Species:	Rattus norvegicus

Alignment Length:	1001	Identity:	265/1001 - (26%)
Similarity:	392/1001 - (39%)	Gaps:	282/1001 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly    50 ADTSTTNAAKKIRRTFSMPRNPFRWTRKFKTAVAVTSSAGAENDN---ESG-----------KNN 100
            |||..| ..|::|:.|::|..  |.||.:...::.|....::.||   ::|           :|.
  Rat   159 ADTIAT-IEKEMRKLFNIPAE--RETRLWNKYMSNTYEQLSKLDNTIQDAGLYQGQVLVIEPQNE 220

  Fly   101 GARERRGSHTAISSCVGGGRGRSGSIVSLSSYEAPFISSTNNNTTTASNGNNNNSSSSNINSKRT 165
            .....|.:..:.||........:.|..|.|.|.:...|...|..:|.|:|.:|
  Rat   221 DGTWPRQTLQSKSSTAPSRNFTTSSKPSASPYSSMSASLIANGDSTNSSGMHN------------ 273

  Fly   166 RALRRSSFRKFLNRIAQHLSSTVNVVSEEKHSPANRIAFIEIVPFYTGCWRRKEANKHGQPSVSG 230
                                                                           ||
  Rat   274 ---------------------------------------------------------------SG 275

  Fly   231 SSSGGGGGAGGPGGGERVPPIGGYQWPADQTPGVMGLKNHGNTCFMNAVLQCLSHTDILAEYFVL 295
            .|.||.|.:......|  ||....|      ||:.||.|.|||||||:.|||||:|..|.|||:.
  Rat   276 VSRGGAGFSASYNCQE--PPSPHIQ------PGLCGLGNLGNTCFMNSALQCLSNTGPLTEYFLK 332

  Fly   296 DQYKADLKRRNKINSRKFGTKGELTEQLANVLKALWTCKNESDHSTSFKAVVDRYGTQFRSSTQH 360
            |:|:|::.|.|.:     |.|||:.|..|.::|.:|:.::.......||..|.|:..||....|.
  Rat   333 DEYEAEINRDNPL-----GMKGEIAEAYAELIKQMWSGRDTHVAPRMFKTQVGRFAPQFSGYQQQ 392

  Fly   361 DAQEFLFWLLDKVHEDLNTASKRRYKSLKNSYGRSDEVIAAETLANHIRCNNSFVQAVFQAQFRS 425
            |:||.|.::||.:|||||...|:.|...|::.||.|.|:|.|...||...|:|.:...|...|:|
  Rat   393 DSQELLAFILDGLHEDLNRVKKKPYLEPKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKS 457

  Fly   426 SLTCPRCEKQSNTFDPFHCISVQLPQLTQQTIFVTVVYMTRLPRQVRMGLRVPAGSPIVALREQL 490
            :|.||.|.|.|.|||||..:::.||....:.:.|.:|......|.::..:.||....|..|.|.|
  Rat   458 TLVCPECAKVSVTFDPFCYLTLPLPLKKDRIMEVFLVPADPHCRPIQYRVTVPLMGAISDLCEAL 522

  Fly   491 QADTGIEGSRMVLVDLNAEGFTRVFYDTQPVETLSSIETIYCIEVPAATPVAKAATPSETGD--- 552
            ...:||....||:.|:....|.::|...:.:..::..:.|:..|:        ..||.:..:
  Rat   523 SKLSGIAAENMVVTDVYNHRFHKIFQMDEGLSHITPRDDIFVYEI--------CTTPMDGSEYIT 579

  Fly   553 --------KSAPTAVTPSPAAAQSQADLLLLVANVYRAKDGADITRFGAPFSMKAPR-----DCS 604
                    ||.|::.:                       .||.:  :|.|..:..|:     :..
  Rat   580 LPVYFREKKSRPSSTS-----------------------SGAVL--YGQPLLVSVPKHRLTLESL 619

  Fly   605 YQDLQKRMLREM-APLLKPEVF---------------SY-------------ATPLAEMFRIRLQ 640
            ||.:.:|:.|.: .||  ||.|               ||             ...|:|:......
  Rat   620 YQAVCERISRYIKQPL--PEEFLSSPLEPGACNGSRGSYEGDEEEMDHQEEGKEQLSEVEESGED 682

  Fly   641 DPSADPDTYLEQVEHP-----LLTEMI--------------DMALSVLSSEAGPQHIKLLLEWSS 686
            ....||....::.:.|     |.|..:              |..|..|:|.:     .|.::|.|
  Rat   683 SQGGDPTETTQKAKGPPRHKRLFTFSLVNSCGTADINSLATDGKLLKLNSRS-----TLAIDWDS 742

  Fly   687 PEAHFISKQQDTEEAVVEHESVARLSASKPSDTAALTLEQCLEHYTKAETLSAEDAWRCPHCQQY 751
            ........:|:: ||..:|.|:::....|   .||:.|.:|:|.:|..|||...|.|.||.|:::
  Rat   743 ETRSLYFDEQES-EACEKHTSMSQPQKKK---KAAIALRECIELFTTMETLGEHDPWYCPTCKKH 803

  Fly   752 LPVVKTLGLWSLPDILVVHFKRFRQHQSKGPQAAKLTTMVKFPLTAFDMSPHLARGVHESASSSL 816
            ....|...|||||.|||||.|||..::.   ...||.|:|:||:.|.:||..:
  Rat   804 QQATKKFDLWSLPKILVVHLKRFSYNRY---WRDKLDTVVEFPVRALNMSEFV------------ 853

  Fly   817 GMGTGIGLGLGLGSNPWKKARSVDSRSSTLNSRCD--AKDTRYDLYAVCYHQGDTLETGHYTAAC 879
                                             ||  |:...|||.||..|.| .:..|||||..
  Rat   854 ---------------------------------CDRAARPYVYDLIAVSNHYG-AMGVGHYTAYA 884

  Fly   880 KNPYDRQWYKFDDQRVSKVPEDDIEQDIINNEAYMLFYQRRSVDAGECSGSSSNSGDHWVSRIAP 944
            ||..:.:||.|||..||...||    .|:...||:||||||.   .||..:||.     ||  .|
  Rat   885 KNRLNGKWYYFDDSSVSLASED----QIVTKAAYVLFYQRRD---DECPSTSSP-----VS--FP 935

  Fly   945 ASSSSASSTSGK----EREPVKLEEN 966
            .|...|..:|.:    |.|...::.|
  Rat   936 GSDGGAKLSSSQQDLGEEEAYTMDTN 961

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Usp15-31	NP_611959.2	Peptidase_C19	<262..>383	CDD:271592	53/120 (44%)
		Peptidase_C19	266..>461	CDD:271592	81/194 (42%)
		Peptidase_C19R	<713..918	CDD:239139	69/206 (33%)
Usp4	NP_001128484.1	Necessary for interaction with SART3. /evidence=ECO:0000250\|UniProtKB:Q13107	27..216		15/59 (25%)
		DUSP	27..125	CDD:197831
		UBP12	35..921	CDD:227847	250/949 (26%)
		Nuclear export signal. /evidence=ECO:0000250\|UniProtKB:P35123	133..141
		Ubiquitin_3	139..226	CDD:291502	16/69 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	219..257		8/37 (22%)
		Required for USP4 activation by providing conformational flexibility between the DUSP and catalytic domains. /evidence=ECO:0000250\|UniProtKB:Q13107	229..295		21/142 (15%)
		Peptidase_C19	302..>486	CDD:271592	80/188 (43%)
		Regulates ubiquitin dissociation. /evidence=ECO:0000250\|UniProtKB:Q13107	384..386		0/1 (0%)
		Necessary for interaction with RBL2. /evidence=ECO:0000250\|UniProtKB:P35123	405..407		0/1 (0%)
		Necessary for interaction with RB1 and RBL2. /evidence=ECO:0000250\|UniProtKB:P35123	459..463		2/3 (67%)
		Interacts with DUSP and ubiquitin-like 1 domains and is required for USP4 activation. /evidence=ECO:0000250\|UniProtKB:Q13107	485..773		59/331 (18%)
		USP7_C2	504..>567	CDD:291217	14/62 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	641..700		7/58 (12%)
		Nuclear localization signal. /evidence=ECO:0000250\|UniProtKB:P35123	765..770		0/4 (0%)
		Peptidase_C19R	<774..919	CDD:239139	66/197 (34%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	924..961		12/43 (28%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1283205at2759
OrthoFinder	1	1.000	-	-		FOG0000596
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.920