Protein Alignment Brca2 and BRCA2

DIOPT Version :10

• Sequence 1: NP_611925.2
• Gene: Brca2 / 37916
• FlyBaseID: FBgn0050169
• Length: 971
• Species: Drosophila melanogaster
• Sequence 2: NP_000050.3
• Gene: BRCA2 / 675
• HGNCID: 1101
• Length: 3418
• Species: Homo sapiens
• Alignment Length: 1109
• Identity: 204/1109 - (18%)
• Similarity: 386/1109 - (34%)
• Gaps: 312/1109 - (28%)

  Fly    28 TVSAAANRSNIIDEMAKICEADRQTFAIARRTRGHERLAVDNSDFVA-VEDLILSYAEPTPEDQV 91
            |...|::...|...:.:|.|:.::||               |:.|.. :.|  .::.:.|...:.
Human   491 TSPVASSFQGIKKSIFRIRESPKETF---------------NASFSGHMTD--PNFKKETEASES 538

  Fly    92 EMIMSDFCSS------PTYAEDEDEPSHESEPWFRFRNKRIRTYSRKRDPKSHKAVQNEKRRGSS 150
            .:.:...||.      |...::...|:..::.....:|..:.:..:|:..|...|:.:|  ....
Human   539 GLEIHTVCSQKEDSLCPNLIDNGSWPATTTQNSVALKNAGLISTLKKKTNKFIYAIHDE--TSYK 601

  Fly   151 GLSVQRDLNTSFTSMACDFDAS------------SQKIH-------------EVLLNLSQYF--- 187
            |..:.:|..:...:.:..|:|:            |..:|             |..|:|:..|
Human   602 GKKIPKDQKSELINCSAQFEANAFEAPLTFANADSGLLHSSVKRSCSQNDSEEPTLSLTSSFGTI 666

  Fly   188 ----SATATASGPTPVPSQIDLPTEARQDSGDECFNAELENL----RDDL--LQSGFTFEASFYE 242
                |...|.|..|.:...:|. .||:      |...:|:..    .|.|  ||.|..      |
Human   667 LRKCSRNETCSNNTVISQDLDY-KEAK------CNKEKLQLFITPEADSLSCLQEGQC------E 718

  Fly   243 NEHEQEGPGTMADTIYGDSMQSVPTTRLRLESGKRNVWSDADSTLKKADVEIAENKKLLM----- 302
            |:.:.:....:.:.:...:...|..:::.        :||.|...:|:.:...||...|:
Human   719 NDPKSKKVSDIKEEVLAAACHPVQHSKVE--------YSDTDFQSQKSLLYDHENASTLILTPTS 775

  Fly   303 ------------GQTQAKNVDIEENTNF-----ILEGIPLSEWLTPMELPEISKDVIKHIPDKKL 350
                        |:...|..|..:..|:     :.:.||:.:......|.|..|:|....|:|.:
Human   776 KDVLSNLVMISRGKESYKMSDKLKGNNYESDVELTKNIPMEKNQDVCALNENYKNVELLPPEKYM 840

  Fly   351 KLEPSSQKE-----------QKSSKDSNASKVRGASKQSCDINTKNEGTTILDQPNAAEQENLL- 403
            ::...|:|.           ||:.:::.:......:..|.::.:.||...:....|  |:.||.
Human   841 RVASPSRKVQFNQNTNLRVIQKNQEETTSISKITVNPDSEELFSDNENNFVFQVAN--ERNNLAL 903

  Fly   404 -NDGELLEEFLFNDWQPMQCSNGP----ST----------------------------SKNAI-- 433
             |..||.|       ..:.|.|.|    ||                            :||::
Human   904 GNTKELHE-------TDLTCVNEPIFKNSTMVLYGDTGDKQATQVSIKKDLVYVLAEENKNSVKQ 961

  Fly   434 -------QGPKENINSINLDDKEQPEKQTPNKSQ------TISSHQLNG-IRKKSFKFIEVSEEM 484
                   |..|.:| |:|:|  :.|||.....::      .||:|...| .|..|.|.|::||.
Human   962 HIKMTLGQDLKSDI-SLNID--KIPEKNNDYMNKWAGLLGPISNHSFGGSFRTASNKEIKLSEH- 1022

  Fly   485 KIKGEEFVDKVVSGLYHFSHKC----NL----------RTEEYSDNHSQVMESTQCAEFKSAPSK 535
            .||..:...|.:...|..|..|    |.          :.:..:...:.:..|...::.|::...
Human  1023 NIKKSKMFFKDIEEQYPTSLACVEIVNTLALDNQKKLSKPQSINTVSAHLQSSVVVSDCKNSHIT 1087

  Fly   536 PIEILDGKETYNA------IAKVDVGEINGKFSPLNNDTIAEPEFCGFRTASNKAIPISEKMKIK 594
            | ::|..|:.:|:      ..|.::.|:    |.:..::.::.||..||..|.    |.:|...:
Human  1088 P-QMLFSKQDFNSNHNLTPSQKAEITEL----STILEESGSQFEFTQFRKPSY----ILQKSTFE 1143

  Fly   595 TAEFMAEFQSKETIQQNDYLVHQPNDKPTSVGLDTALKRSIESSEEMRSKASKLVVVDTTMREPH 659
            ..|.........:.:..|..:|...:.|:...:|::  :..|.:.|::.|.:.|:..|.......
Human  1144 VPENQMTILKTTSEECRDADLHVIMNAPSIGQVDSS--KQFEGTVEIKRKFAGLLKNDCNKSASG 1206

  Fly   660 QPTLDPVCRDLNESQFFGFRTASNKAIEI-TEAMEKRGAMFLAQSKATDQLNGWQPSDFPDVLPT 723
            ..|      |.||..|.||.:|....:.: |||::|...:|               ||..:: ..
Human  1207 YLT------DENEVGFRGFYSAHGTKLNVSTEALQKAVKLF---------------SDIENI-SE 1249

  Fly   724 SPNNEIHSINVENNKA------------VNTKTVSETEFFGFRTASNK-GIVISENTKIKVAQFM 775
            ..:.|:|.|::.::|.            .|.|||||        .:|| .:::..|.::....|:
Human  1250 ETSAEVHPISLSSSKCHDSVVSMFKIENHNDKTVSE--------KNNKCQLILQNNIEMTTGTFV 1306

  Fly   776 SEF---QAADASTDSNQPTVISEESRNIDAKFVDEAAAEDSANKPTFCNVQSLKNTSDI----EH 833
            .|.   ...:...:.|:.|..|..|.|:      |....||:...|.|   ..|:.:|:    :|
Human  1307 EEITENYKRNTENEDNKYTAASRNSHNL------EFDGSDSSKNDTVC---IHKDETDLLFTDQH 1362

  Fly   834 -----FKHDFFVEHSAKEEHPLCSQPLVRTPRRSQEIHSSLSQLAGKSPLDQATKKSVIARRNLL 893
                 ....|..|.:.:.:..|.....:...:..:..|.:.|           .|:.:.|.:...
Human  1363 NICLKLSGQFMKEGNTQIKEDLSDLTFLEVAKAQEACHGNTS-----------NKEQLTATKTEQ 1416

  Fly   894 SLKRKRKIILSTETS-----------TSCALPTMER----FAPKPSSTSTPLADRDLNRSKDCTK 943
            ::|       ..|||           .|.|..:..:    |..||........:.:|:  .|..|
Human  1417 NIK-------DFETSDTFFQTASGKNISVAKESFNKIVNFFDQKPEELHNFSLNSELH--SDIRK 1472

  Fly   944 NRQD 947
            |:.|
Human  1473 NKMD 1476

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Brca2	NP_611925.2	None
BRCA2	NP_000050.3	Interaction with PALB2	1..40
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	37..68
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	358..381
		Interaction with NPM1. /evidence=ECO:0000269|PubMed:21084279	639..1000		72/393 (18%)
		BRCA2 1	1002..1036		11/34 (32%)
		Interaction with RAD51. /evidence=ECO:0000250|UniProtKB:P97929	1003..2082		105/545 (19%)
		BRCA2	1005..1033	CDD:459881	9/28 (32%)
		BRCA2	1216..1243	CDD:459881	9/41 (22%)
		Interaction with POLH. /evidence=ECO:0000269|PubMed:24485656	1338..1781		28/162 (17%)
		Required for stimulation of POLH DNA polymerization activity	1410..1595		15/76 (20%)
		BRCA2	1424..1452	CDD:459881	4/27 (15%)
		BRCA2	1522..1548	CDD:459881
		BRCA2	1667..1695	CDD:459881
		BRCA2	1974..2002	CDD:459881
		BRCA2 8	2051..2085
		BRCA2	2055..2082	CDD:459881
		Interaction with HSF2BP. /evidence=ECO:0000269|PubMed:31242413	2270..2337
		Interaction with FANCD2	2350..2545
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	2430..2450
		Interaction with SEM1. /evidence=ECO:0000269|PubMed:10373512, ECO:0000269|PubMed:16205630	2481..2832
		BRCA-2_helical	2481..2667	CDD:462700
		BRCA-2_OB1	2670..2796	CDD:462678
		Nuclear export signal, masked by interaction with SEM1. /evidence=ECO:0000269|PubMed:24013206	2682..2698
		BRCA2DBD_OB2	2804..3054	CDD:239940
		BRCA-2_OB3	3052..3186	CDD:462679
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	3393..3418