DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Brca2 and Brca2

DIOPT Version :9

Sequence 1:	NP_611925.2	Gene:	Brca2 / 37916	FlyBaseID:	FBgn0050169	Length:	971	Species:	Drosophila melanogaster
Sequence 2:	NP_001074470.1	Gene:	Brca2 / 12190	MGIID:	109337	Length:	3329	Species:	Mus musculus

Alignment Length:	1242	Identity:	263/1242 - (21%)
Similarity:	429/1242 - (34%)	Gaps:	399/1242 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 DQNGASGSH--------PNRLSQGRGAHARERGATVSAAANRSNIIDEMAKICEADRQTFAIARR 58
            |.:|...|.        ||.|..|            ..|.:.|.  ::...:|...|:|..:.:.
Mouse   264 DSSGKRNSFKDCLRKPIPNILEDG------------ETAVDTSE--EDSFSLCFPKRRTRNLQKM 314

  Fly    59 TRGHERLAV---DNSDFVAVEDLILSYAEPTPEDQ----VEMIMSDFCSSPTYAEDEDEPSHESE 116
            ..|..|..:   ..:|.::.|      |....:|:    .||.:.:        .|..:|...|:
Mouse   315 RMGKTRKKIFSETRTDELSEE------ARRQTDDKNSFVFEMELRE--------SDPLDPGVTSQ 365

  Fly   117 PWFRFRNKRIRTYSRKRDPKSHKAVQ-NEKRRGSSGLSVQRDLNTSFTSMACDFDASSQKIHEVL 180
            ..|..:|:.|          .::||| ::.|...|.||...:..|...::. ...:.||.|.|..
Mouse   366 KPFYSQNEEI----------CNEAVQCSDSRWSQSNLSGLNETQTGKITLP-HISSHSQNISEDF 419

  Fly   181 LNLSQYFSATATASGPTPVPSQIDLPT------------------EARQDS--GDE--------- 216
            :::.:..:.:.|:....|..|.:..|.                  |:.:||  |.:
Mouse   420 IDMKKEGTGSITSEKSLPHISSLPEPEKMFSEETVVDKEHEGQHFESLEDSIAGKQMVSRTSQAA 484

  Fly   217 CFNAELE----NLRDDLLQS-GFTFEASF----YENEHEQE--GPGTMA------DTIYGDSMQ- 263
            |.:..:.    .:|:.|.:: |..|..|.    :..|||..  |.|.:.      |:|...|:.
Mouse   485 CLSPSIRKSIFKMREPLDETLGTVFSDSMTNSTFTEEHEASACGLGILTACSQREDSICPSSVDT 549

  Fly   264 -SVPTTRLRLESGKRNVWSDADSTLKKAD-VEIAENKKLLMGQTQAKNVDIEE---NTNFILEGI 323
             |.|||           .:|..:|:|.|. :...:|||.....:.:.:..::.   .|:..||..
Mouse   550 GSWPTT-----------LTDTSATVKNAGLISTLKNKKRKFIYSVSDDASLQGKKLQTHRQLELT 603

  Fly   324 PLSEWL--TPMELPEISKDVIKHIP---DKKLKL-----EPSSQKEQKSSKDSNASKVRGASKQS 378
            .||..|  :..|:|....:|...||   |||..|     |||......::.....|.:.....|
Mouse   604 NLSAQLEASAFEVPLTFTNVNSGIPDSSDKKRCLPNDPEEPSLTNSFGTATSKEISYIHALISQ- 667

  Fly   379 CDINTKNEGTTILDQP---NAAEQENLLNDGELLEEFLFNDWQPMQCSNG----------PSTSK 430
             |:|.| |...|.::|   .|.|.:.||.   |.|....||.:..:.|||          ||
Mouse   668 -DLNDK-EAIVIEEKPQPYTAREADFLLC---LPERTCENDQKSPKVSNGKEKVLVSACLPS--- 724

  Fly   431 NAIQGPKENINSINLDDKEQP---------EKQTPNKSQTISSHQLNGIRKKSFKFIEVSEEMKI 486
             |:|     ::||:.:.:|.|         .|.||:....:|...:.. |:|..|..|     |:
Mouse   725 -AVQ-----LSSISFESQENPLGDHNGTSTLKLTPSSKLPLSKADMVS-REKMCKMPE-----KL 777

  Fly   487 KGEEFVDKVVSGLYHFSHKCNLRTEEYSDNHSQVMESTQCAEFKSAP------SKPIEILDG-KE 544
            :.|             |.|.|:   |.|.|..:|.|....:|....|      ...||:... |.
Mouse   778 QCE-------------SCKVNI---ELSKNILEVNEICILSENSKTPGLLPPGENIIEVASSMKS 826

  Fly   545 TYNAIAKVDVGEINGKFSP--------LNNDTIAEPEFCGFRTASNKAIPISEKMKIKTAEFMAE 601
            .:|..||: |.:.:.|.||        :|::.:. |:     :.:|.|..::.|..      ..:
Mouse   827 QFNQNAKI-VIQKDQKGSPFISEVAVNMNSEELF-PD-----SGNNFAFQVTNKCN------KPD 878

  Fly   602 FQSKETIQQNDYLVHQ--PNDKPTSVGLDTALKRSIESSEEMRSKASKLVVV-DTTMR-----EP 658
            ..|...:|:.| |.|.  |:.|.:.:.:|..:..:..:...:...:..|.|| |.|.:     |.
Mouse   879 LGSSVELQEED-LSHTQGPSLKNSPMAVDEDVDDAHAAQVLITKDSDSLAVVHDYTEKSRNNIEQ 942

  Fly   659 HQ-----------------------------PTLDPVCRDLNESQFFGFRTASNKAIEITEAMEK 694
            ||                             ..||||   ||.:....|||||||.|:::|...|
Mouse   943 HQKGTEDKDFKSNSSLNMKSDGNSDCSDKWSEFLDPV---LNHNFGGSFRTASNKEIKLSEHNVK 1004

  Fly   695 RGAMF----------------------LAQSK----------------ATDQLNGWQPS-DFPDV 720
            :..||                      ||..|                :|...|....| :..|.
Mouse  1005 KSKMFFKDIEEQYPTRLACIDIVNTLPLANQKKLSEPHIFDLKSVTTVSTQSHNQSSVSHEDTDT 1069

  Fly   721 LP--TSPNNEIHSINVENNK--------AVNTKTVSETEFFGFRTASNKGIVISENTKIKVAQFM 775
            .|  .|...:.||.|:..::        .:..::.|:.||..||..|:    |::||.......|
Mouse  1070 APQMLSSKQDFHSNNLTTSQKAEITELSTILEESGSQFEFTQFRKPSH----IAQNTSEVPGNQM 1130

  Fly   776 SEFQAAD---ASTDSNQPTVISEESRNIDAKFVDEAAA--------EDSANKPT----------- 818
            .....|.   ..||.:.|...|....:...:|...|..        ||:.||.|
Mouse  1131 VVLSTASKEWKDTDLHLPVDPSVGQTDHSKQFEGSAGVKQSFPHLLEDTCNKNTSCFLPNINEME 1195

  Fly   819 ---FCNV-------------QSLKNTSDIEHFKHDFFVEHSAKEEHPLCSQPLVRTPR--RSQEI 865
               ||:.             :::|..||||:.:     |.|||.           .||  .|...
Mouse  1196 FGGFCSALGTKLSVSNEALRKAMKLFSDIENSE-----EPSAKV-----------GPRGFSSSAH 1244

  Fly   866 HSSLS-------QLAGKSPLDQATKKSVIARRNLLSL-------KRKRKIILST---ETSTSCAL 913
            |.|::       |...|| .|:.:.|..:..:|.:.:       :...|.|.:|   ::.||...
Mouse  1245 HDSVASVFKIKKQNTEKS-FDEKSSKCQVTLQNNIEMTTCIFVGRNPEKYIKNTKHEDSYTSSQR 1308

  Fly   914 PTMERFAPKPSSTSTPL----------ADRDLNRSKDCT-----KNRQDAEDMSPI-CMQPKKSR 962
            ..:|......||||.|:          ||:.....:.||     :|.|..|::|.: |::..|:.
Mouse  1309 NNLENSDGSMSSTSGPVYIHKGDSDLPADQGSKCPESCTQYAREENTQIKENISDLTCLEIMKAE 1373

  Fly   963 RLGLSRS 969
            ...:..|
Mouse  1374 ETCMKSS 1380

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Brca2	NP_611925.2	None
Brca2	NP_001074470.1	Interaction with PALB2. /evidence=ECO:0000250	1..40
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	37..69
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	207..241
		Interaction with NPM1. /evidence=ECO:0000250	628..979		102/463 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	628..650		6/21 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	934..965		14/52 (27%)
		BRCA2 1	981..1015		9/43 (21%)
		Interaction with RAD51. /evidence=ECO:0000269\|PubMed:30760716	982..2035		71/333 (21%)
		BRCA2	982..1012	CDD:395509	6/39 (15%)
		BRCA2	1193..1223	CDD:395509	9/35 (26%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1296..1340
		BRCA2	1395..1423	CDD:395509
		BRCA2	1492..1522	CDD:395509
		BRCA2	1624..1654	CDD:395509
		BRCA2	1927..1955	CDD:395509
		BRCA2 7	2004..2038
		BRCA2	2005..2035	CDD:395509
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2073..2099
		Interaction with HSF2BP. /evidence=ECO:0000269\|PubMed:30760716, ECO:0000269\|PubMed:32345962	2219..2285
		Interaction with FANCD2. /evidence=ECO:0000250	2298..2466
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2361..2393
		Interaction with SEM1. /evidence=ECO:0000250\|UniProtKB:P51587	2402..2753
		BRCA-2_helical	2402..2588	CDD:401201
		BRCA-2_OB1	2591..2714	CDD:401152
		Nuclear export signal, masked by interaction with SEM1. /evidence=ECO:0000250\|UniProtKB:P51587	2603..2619
		BRCA2DBD_OB2	2725..2973	CDD:239940
		BRCA-2_OB3	2974..3102	CDD:401153
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	3221..3257
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	3273..3329
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG4751
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0006415
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.900