Protein Alignment mAChR-A and Hrh3

DIOPT Version :10

• Sequence 1: NP_523844.2
• Gene: mAChR-A / 37892
• FlyBaseID: FBgn0000037
• Length: 805
• Species: Drosophila melanogaster
• Sequence 2: NP_598610.1
• Gene: Hrh3 / 99296
• MGIID: 2139279
• Length: 445
• Species: Mus musculus
• Alignment Length: 728
• Identity: 154/728 - (21%)
• Similarity: 240/728 - (32%)
• Gaps: 321/728 - (44%)

  Fly    73 GLYDPYSGMYGN--QTNGTIGFETKGPRYSLASMVVMGFVAAILSTVTVAGNVMVMISFKIDKQL 135
            ||.:....:.|.  ...|..||       |.|...|:..:.|:|...||.||.:||::|..|..|
Mouse     8 GLMNASGALAGEAAAAGGARGF-------SAAWTAVLAALMALLIVATVLGNALVMLAFVADSSL 65

  Fly   136 QTISNYFLFSLAIADFAIGAISMPLFAVTTILGYWPLGPIVCDTWLALDYLASNASVLNLLIISF 200
            :|.:|:||.:|||:||.:||..:||:....:.|.|..|..:|..||.:|||...:||.|:::||:
Mouse    66 RTQNNFFLLNLAISDFLVGAFCIPLYVPYVLTGRWTFGRGLCKLWLVVDYLLCASSVFNIVLISY 130

  Fly   201 DRYFSVTRPLTYRAKR-TTNRAAVMIGAAWGISLLLWPPWIYSWPYIEGKRTVPKDECYIQFIET 264
            ||:.||||.::|||:: .|.||...:...|.::.||:.|.|.||.|:.|..::|:..||.:|. .
Mouse   131 DRFLSVTRAVSYRAQQGDTRRAVRKMALVWVLAFLLYGPAILSWEYLSGGSSIPEGHCYAEFF-Y 194

  Fly   265 NQYITFGTALAAFYFPVTIMCF----LYWRIWRETKKRQKDLPNLQAGKKDSSKRSNSSDENTVV 325
            |.|.....:...|:.|...:.|    :|..|.|.|:.|
Mouse   195 NWYFLITASTLEFFTPFLSVTFFNLSIYLNIQRRTRLR--------------------------- 232

  Fly   326 NHASGGLLAFAQVGGNDHDTWRRPRSESSPDAESVYMTNMVIDSGYHGMHSRKSSIKSTNTIKKS 390
                                                     :|
Mouse   233 -----------------------------------------LD---------------------- 234

  Fly   391 YTCFGSIKEWCIAWWHSGREDSDDFAYEQEEPSDLGYATPVTIETPLQSSVSRCTSMNVMRDNYS 455
                                                                             
Mouse   235 ----------------------------------------------------------------- 234

  Fly   456 MGGSVSGVRPPSILLSDVSPTPLPRPPLASISQLQEMSAVTASTTANVNTSGNGNGAINNNNNAS 520
             ||..:|..||    .|..|:|.|.||                :.......|:|...
Mouse   235 -GGREAGPEPP----PDAQPSPPPAPP----------------SCWGCWPKGHGEAM-------- 270

  Fly   521 HNGNGAVNGNGAGN-GSGIGLGTTGNATHRDSRTLPVINRINSRSVSQDSVYTILIRLPSDGASS 584
                 .::..|.|. |.|:   .||.|
Mouse   271 -----PLHRYGVGEAGPGV---ETGEA-------------------------------------- 289

  Fly   585 NAANGGGGGPGAGAAASASLSMQGDCAPSIKMIHEDGPTTTAAAAPLASAAATRRPLP----SRD 645
                |.|||.|.|||||                    ||:::.    :|:..|.||..    |:.
Mouse   290 ----GLGGGSGGGAAAS--------------------PTSSSG----SSSRGTERPRSLKRGSKP 326

  Fly   646 SEFSLPLGRRMSHAQHDARLLNAKVIPKQLGKAGGGAAGGGVGGAHALMNARNAAKKKKKSQEKR 710
            |..|..|.:||.                                    |.:::..::.:.|::|
Mouse   327 SASSASLEKRMK------------------------------------MVSQSITQRFRLSRDK- 354

  Fly   711 QESKAAKTLSAILLSFIITWTPYNILVLIKPLTTC-SDCIPTELWDFFYALCYINSTINPMCYAL 774
               |.||:|:.|:..|.:.|.||.:|::|:  ..| ..|:|...::..:.|.:.||.:||:.|.|
Mouse   355 ---KVAKSLAIIVSIFGLCWAPYTLLMIIR--AACHGHCVPDYWYETSFWLLWANSAVNPVLYPL 414

  Fly   775 CNATFRRTYVRIL 787
            |:.:|||.:.::|
Mouse   415 CHYSFRRAFTKLL 427

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
mAChR-A	NP_523844.2	7tmA_mAChR_DM1-like	105..>300	CDD:320428	74/199 (37%)
		TM helix 1	106..132	CDD:320428	10/25 (40%)
		TM helix 2	139..165	CDD:320428	12/25 (48%)
		TM helix 3	177..207	CDD:320428	14/29 (48%)
		TM helix 4	219..242	CDD:320428	7/22 (32%)
		TM helix 5	265..294	CDD:320428	7/32 (22%)
		7tm_GPCRs	<707..783	CDD:475119	26/76 (34%)
		TM helix 6	716..738	CDD:410628	9/21 (43%)
		TM helix 7	751..776	CDD:410628	7/24 (29%)
Hrh3	NP_598610.1	7tmA_Histamine_H3R	48..423	CDD:320423	142/675 (21%)
		TM helix 2	69..96	CDD:320423	12/26 (46%)
		TM helix 3	107..137	CDD:320423	14/29 (48%)
		TM helix 4	152..170	CDD:320423	4/17 (24%)
		TM helix 5	195..225	CDD:320423	6/29 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	234..259		12/132 (9%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	273..336		27/131 (21%)
		TM helix 6	351..381	CDD:320423	12/33 (36%)
		TM helix 7	391..416	CDD:320423	7/24 (29%)