Protein Alignment mAChR-A and chrm1b

DIOPT Version :10

• Sequence 1: NP_523844.2
• Gene: mAChR-A / 37892
• FlyBaseID: FBgn0000037
• Length: 805
• Species: Drosophila melanogaster
• Sequence 2: XP_001334664.5
• Gene: chrm1b / 794658
• ZFINID: ZDB-GENE-070705-188
• Length: 487
• Species: Danio rerio
• Alignment Length: 749
• Identity: 206/749 - (27%)
• Similarity: 294/749 - (39%)
• Gaps: 285/749 - (38%)

  Fly    65 NLTSTAASGLYDPYSGMYGNQTNGTIGFETKGPRYSLASMVVMGFVAAILSTVTVAGNVMVMISF 129
            |.||...:...||..|                  :....:|::..|...||.:|:.||.:|:||.
Zfish     4 NCTSQTTNLTMDPLGG------------------HQEWEVVLIVLVTGPLSLITILGNSLVVISI 50

  Fly   130 KIDKQLQTISNYFLFSLAIADFAIGAISMPLFAVTTILGYWPLGPIVCDTWLALDYLASNASVLN 194
            :::.||:|||||:|.|||:||..:|.:||.|:....|||.|.|||:.||.||.|||:.|||||:|
Zfish    51 RVNSQLRTISNYYLLSLAVADLILGTVSMNLYTAYVILGRWTLGPLACDLWLTLDYVVSNASVMN 115

  Fly   195 LLIISFDRYFSVTRPLTYRAKRTTNRAAVMIGAAWGISLLLWPPWIYSWPYIEGKRT-VPKDECY 258
            ||:||||||||||||||||.|||...|||:|..||.||.:||.|.|..||::.|:.: .....|.
Zfish   116 LLVISFDRYFSVTRPLTYRTKRTPKTAAVLIALAWVISFILWGPAILFWPHVMGRPSGAEAHVCS 180

  Fly   259 IQFIETNQYITFGTALAAFYFPVTIMCFLYWRIWRETKKRQKDLPNLQAGKKDSSKRSNSSDENT 323
            |.|::... ||:|||:||||.||:||..|||||:.|.:.|.|.|.......|::::.....|:.:
Zfish   181 IPFLKVPP-ITYGTAIAAFYLPVSIMIILYWRIYWEIENRAKGLAGFVGSVKNATEILEGLDKQS 244

  Fly   324 VVNHASGGLLAFAQVGGNDHDTWRRPRSESSPDAESVYMTNMVIDSGYHGMHSRKSSIKSTNTIK 388
            :...:|...|:.::.|                                   .|:|...||..
Zfish   245 MHQTSSKSTLSISREG-----------------------------------ISKKGQGKSVG--- 271

  Fly   389 KSYTCFGSIKEWCIAWWHSGREDSDDFAYEQEEPSDLGYATPVTIETPLQSSVSRCTSMNVMRDN 453
                        |..|                                            ::|:.
Zfish   272 ------------CFTW--------------------------------------------ILRNK 280

  Fly   454 YSMGGSVSGVRPPSILLSDVSPTPLPRPPLASISQLQEMSAVTASTTANVNTSGNGNGAINNNNN 518
            .:...|.                   :.|.:|.|.               ||....:..::::.:
Zfish   281 TAAHESY-------------------KEPYSSSSW---------------NTEDVDDSVLSSSTD 311

  Fly   519 ASHNGNGAVNGNGAGNGSGIGLGTTGNATHRDSRTLPVINRINSRSVSQDSVYTILIRLPSDGAS 583
            ..|..|                                 |::.:.|.:..:     |:|......
Zfish   312 EEHEPN---------------------------------NKLKAPSSNPSA-----IKLKDLHCR 338

  Fly   584 SNAANGGGGGPGAGAAASASLSMQGDCAPS---IKMIHEDGPTTTAAAAPLASAAATRRPLPSRD 645
            |:...|                :|...:|:   .|..|                      |..:.
Zfish   339 SDDTKG----------------VQEQNSPAERLTKQCH----------------------LKPKK 365

  Fly   646 SEFSLPLGRRMSHAQHDARLLNAKVIPKQLGKAGGGAAGGGVGGAHALMNARNAAKKKKKSQEKR 710
            |:.|:   |:...|:|...|:                                           .
Zfish   366 SDHSI---RQQLRAKHRIILI-------------------------------------------I 384

  Fly   711 QESKAAKTLSAILLSFIITWTPYNILVLIKPLTTCSDCIPTELWDFFYALCYINSTINPMCYALC 775
            :|.|||:|||||||:||:|||||||:|    |.:.|.|:|.:||...|.|||:|||:||||||||
Zfish   385 KEKKAARTLSAILLAFILTWTPYNIMV----LASISYCVPEKLWQLGYWLCYVNSTVNPMCYALC 445

  Fly   776 NATFRRTYVRILTC------KWHTR--NREGMVR 801
            |.:||.|:..:|.|      ||.|.  ||...||
Zfish   446 NESFRVTFKSLLLCRGGDKRKWDTNSWNRHAFVR 479

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
mAChR-A	NP_523844.2	7tmA_mAChR_DM1-like	105..>300	CDD:320428	106/195 (54%)
		TM helix 1	106..132	CDD:320428	9/25 (36%)
		TM helix 2	139..165	CDD:320428	13/25 (52%)
		TM helix 3	177..207	CDD:320428	23/29 (79%)
		TM helix 4	219..242	CDD:320428	12/22 (55%)
		TM helix 5	265..294	CDD:320428	18/28 (64%)
		7tm_GPCRs	<707..783	CDD:475119	45/75 (60%)
		TM helix 6	716..738	CDD:410628	17/21 (81%)
		TM helix 7	751..776	CDD:410628	16/24 (67%)
chrm1b	XP_001334664.5	None