DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment mAChR-A and chrm3a

DIOPT Version :9

Sequence 1:	NP_523844.2	Gene:	mAChR-A / 37892	FlyBaseID:	FBgn0000037	Length:	805	Species:	Drosophila melanogaster
Sequence 2:	XP_021328763.1	Gene:	chrm3a / 571679	ZFINID:	ZDB-GENE-090410-4	Length:	595	Species:	Danio rerio

Alignment Length:	791	Identity:	249/791 - (31%)
Similarity:	341/791 - (43%)	Gaps:	280/791 - (35%)

- Green bases have known domain annotations that are detailed below.

  Fly    49 YSPG-------YPGTTL-----------LTALFE-NLTSTAASG-LYDPYSGMYGNQTNGTIGFE 93
            :||.       ||.:||           |||:|: |.::..|.| .|||..|
Zfish    11 FSPSDTLGVGMYPASTLWPLLQNTSLVNLTAVFKLNGSNAEAEGQSYDPLGG------------- 62

  Fly    94 TKGPRYSLASMVVMGFVAAILSTVTVAGNVMVMISFKIDKQLQTISNYFLFSLAIADFAIGAISM 158
                 :||..::::.....:||.:|:.||::||:|||:::||:|::||||.|||:||..||.|||
Zfish    63 -----HSLWQVILIVVFTGLLSLITIIGNILVMVSFKVNRQLKTVNNYFLLSLAVADLIIGVISM 122

  Fly   159 PLFAVTTILGYWPLGPIVCDTWLALDYLASNASVLNLLIISFDRYFSVTRPLTYRAKRTTNRAAV 223
            .|:....::|.|.:|...||.|||:||:||||||:|||:||||||||:||||||||||||.||.|
Zfish   123 NLYTAYIVMGQWAMGNWACDLWLAIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTKRAGV 187

  Fly   224 MIGAAWGISLLLWPPWIYSWPYIEGKRTVPKDECYIQFIETNQYITFGTALAAFYFPVTIMCFLY 288
            |||.||.:||:||.|.|..|.|..|:||||:|:|||||: :...|||.||:||||.|||||..||
Zfish   188 MIGLAWFVSLILWAPAILFWQYFVGQRTVPQDKCYIQFL-SEPIITFCTAMAAFYLPVTIMSVLY 251

  Fly   289 WRIWRETKKRQKDLPNLQAG---------------KKDSSKRSNSSDENTVVNHASGGLLAFA-- 336
            |||::||:.|.::|..||..               ....|.||.||.|.....|....:.:.:
Zfish   252 WRIYKETENRSRELAGLQGSGGRFGGVERPRFHLHATRGSSRSCSSFELGQPGHKKASVHSLSGR 316

  Fly   337 ------QVGGNDHDTWRRPRSESSPDAESVYMTNMVIDSGYHGMHSRKSSIKSTNTIKKSYTCFG 395
                  :.||:|.....|...:||.|:   :..|   |:|
Zfish   317 FHCWGWKSGGSDKSGPNREADQSSSDS---WNNN---DAG------------------------- 350

  Fly   396 SIKEWCIAWWHSGREDSDDFAYEQEEPSDLGYATPVTIETP-----LQSSVSRCTSMNVMRDNYS 455
                  ::..|||..|.|:.|     ||.......:.:..|     :.|.|:.|..::...|
Zfish   351 ------LSADHSGSSDEDESA-----PSTTRAIFSIVLSLPGVRAAVNSQVTSCEELDTEED--- 401

  Fly   456 MGGSVSGVRPPSILLSDVSPTPLPRPPLASISQLQEMSAVTASTTANVNTSGNGNGAINNNNNAS 520
                                      ||.|..:                 ..:.:|:|:.:
Zfish   402 --------------------------PLRSAEE-----------------KDSRDGSISRS---- 419

  Fly   521 HNGNGAVNGNGAGNGSGIGLGTTGNATHRDSRTLPVINRINSRSVSQDSVYTILIRLPSDGASSN 585
                 ..|||                    .|.:..::::.|.|..|          |:...:::
Zfish   420 -----VTNGN--------------------KRFVGGMSKVQSMSAIQ----------PTTDTTTD 449

  Fly   586 AANGGGGGPGAGAAASASLSMQGDCAPSIKMIHEDGPTTTAAAAPLASAAATRRPLPSRDSEFSL 650
            :.|.....|.|                         |.|...|| ||...|.|
Zfish   450 STNTTIKSPSA-------------------------PITFKEAA-LAKRFAAR------------ 476

  Fly   651 PLGRRMSHAQHDARLLNAKVIPKQLGKAGGGAAGGGVGGAHALMNARNAAKKKKKSQEKRQESKA 715
                                                         ||....|:|: ....:|.||
Zfish   477 ---------------------------------------------ARTQITKRKR-MSLIKEKKA 495

  Fly   716 AKTLSAILLSFIITWTPYNILVLIKPLTTCSDCIPTELWDFFYALCYINSTINPMCYALCNATFR 780
            |:||||||.:||||||||||:||:.  |.|:.|||..||...|.|||:|||:|||||||||.|||
Zfish   496 AQTLSAILFAFIITWTPYNIMVLVN--TFCNGCIPENLWALGYWLCYVNSTVNPMCYALCNKTFR 558

  Fly   781 RTYVRILTCKW 791
            .|:..||.|:|
Zfish   559 STFKMILLCRW 569

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mAChR-A	NP_523844.2	7tm_4	117..>241	CDD:304433	78/123 (63%)
mAChR-A	NP_523844.2	7tm_1	121..>314	CDD:278431	116/207 (56%)
chrm3a	XP_021328763.1	7tm_GPCRs	66..>258	CDD:333717	113/192 (59%)
		TM helix 1	71..95	CDD:320095	9/23 (39%)
		TM helix 2	104..126	CDD:320095	15/21 (71%)
		TM helix 3	142..164	CDD:320095	16/21 (76%)
		TM helix 4	187..203	CDD:320095	10/15 (67%)
		TM helix 5	229..252	CDD:320095	15/22 (68%)
		7tm_GPCRs	<462..570	CDD:333717	64/169 (38%)
		TM helix 5	462..483	CDD:320095	9/78 (12%)
		TM helix 6	493..518	CDD:320095	19/24 (79%)
		TM helix 7	529..554	CDD:320095	16/24 (67%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	345	1.000	Domainoid score	I1022
eggNOG	1	0.900	-	-		E1_KOG4220
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	385	1.000	Inparanoid score	I1990
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1245472at2759
OrthoFinder	1	1.000	-	-		FOG0001308
OrthoInspector	1	1.000	-	-		otm24829
orthoMCL	1	0.900	-	-		OOG6_108988
Panther	1	1.100	-	-	LDO	PTHR24247
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X324
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	12	11.870