Protein Alignment mAChR-A and chrm5b

DIOPT Version :10

• Sequence 1: NP_523844.2
• Gene: mAChR-A / 37892
• FlyBaseID: FBgn0000037
• Length: 805
• Species: Drosophila melanogaster
• Sequence 2: XP_068071586.2
• Gene: chrm5b / 561491
• ZFINID: ZDB-GENE-041001-169
• Length: 505
• Species: Danio rerio
• Alignment Length: 699
• Identity: 224/699 - (32%)
• Similarity: 303/699 - (43%)
• Gaps: 228/699 - (32%)

  Fly    99 YSLASMVVMGFVAAILSTVTVAGNVMVMISFKIDKQLQTISNYFLFSLAIADFAIGAISMPLFAV 163
            |||..::.:..|:||:|.:|:.|||:||:|||::.||:|::||:|.|||.||..||..||.|:..
Zfish    22 YSLLEVITIATVSAIVSLITIVGNVLVMLSFKVNSQLKTVNNYYLLSLAFADLIIGVFSMNLYTC 86

  Fly   164 TTILGYWPLGPIVCDTWLALDYLASNASVLNLLIISFDRYFSVTRPLTYRAKRTTNRAAVMIGAA 228
            ..::|||.||...||.||||||:||||||:|||:||||||||:|||||||||||..||.:|||.|
Zfish    87 YILMGYWSLGNTACDLWLALDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTPKRAGIMIGMA 151

  Fly   229 WGISLLLWPPWIYSWPYIEGKRTVPKDECYIQFIETNQYITFGTALAAFYFPVTIMCFLYWRIWR 293
            |.||.:||.|.|..|.|..|:|.||.|:|.|||. :...||||||:||||.||:||..||.:|::
Zfish   152 WLISFILWAPPILCWQYFVGERKVPLDQCQIQFF-SEPIITFGTAIAAFYVPVSIMTILYCKIYK 215

  Fly   294 ETKKRQKDLPNLQAGKKDSSKRSNSSDENTVVNHASGGLLAFAQVGGNDHDTWRRPRSESSPDAE 358
            ||:||.|:|..||
Zfish   216 ETEKRTKNLAELQ---------------------------------------------------- 228

  Fly   359 SVYMTNMVIDSGYHGMHSRKSSIKSTNTIKKSYTCFGSIKEWCIAWWHSGREDSDDFAYEQEEPS 423
                       ||..:.|:... |....|.:.:: |.:.::...|.|.|               |
Zfish   229 -----------GYPSLDSQDGP-KVRKPILRCFS-FKNKRDGTQASWSS---------------S 265

  Fly   424 DLGYATPVTIET-PLQSSVSRCTSMNVMRDNYSMGGSVSGVRPPSILLSDVSPTPLPRPPLASIS 487
            :..|.|..|::: .|.:...:.|::|....:.....|||...|.....:.       .||::...
Zfish   266 NQSYVTRTTLQSDDLWTKSEQVTTLNSYTSSEDDDRSVSESTPRESFKNQ-------EPPVSKNG 323

  Fly   488 QLQEMSAVTASTTANVNTSGNGNGAINNNNNASHNGNGAVNGNGAGNGSGIGLGTTGNATHRDSR 552
            ||                                                        ..:.||:
Zfish   324 QL--------------------------------------------------------VCYEDSK 332

  Fly   553 TLPVINRINSRSVSQDSVYTILIRLPSDGASSNAANGGGGGPGAGAAASASLSMQGDCAPSIKMI 617
            .|                        ||.|..:..|.           ...||.:      .|.|
Zfish   333 YL------------------------SDVAKCSQTNN-----------KKCLSYK------FKPI 356

  Fly   618 HEDGPTTTAAAAPLASAAATRRPLPSRDSEFSLPLGRRMSHAQHDARLLNAKVIPKQLGKAGGGA 682
            ..|       ...|...:.:.|..|.:.|:...|.....|....|.   |.|:
Zfish   357 ISD-------ITVLQGNSESERANPDQSSDQQAPSSTSSSTKPEDP---NLKI------------ 399

  Fly   683 AGGGVGGAHALMNARNAAKKKKKSQEKRQESKAAKTLSAILLSFIITWTPYNILVLIKPLTTCSD 747
                             ...|:|.....:|.|||:|||||||:|::|||||||:|||.  |.||:
Zfish   400 -----------------QMTKRKRMVLIKEKKAAQTLSAILLAFLLTWTPYNIMVLIS--TFCSN 445

  Fly   748 CIPTELWDFFYALCYINSTINPMCYALCNATFRRTYVRILTCKWHTRNR 796
            ||||.||...|.|||:|||:|||||||||.||::|:..::.||| .:||
Zfish   446 CIPTSLWQLGYWLCYVNSTVNPMCYALCNKTFQKTFWMLILCKW-KKNR 493

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
mAChR-A	NP_523844.2	7tmA_mAChR_DM1-like	105..>300	CDD:320428	115/194 (59%)
		TM helix 1	106..132	CDD:320428	13/25 (52%)
		TM helix 2	139..165	CDD:320428	13/25 (52%)
		TM helix 3	177..207	CDD:320428	25/29 (86%)
		TM helix 4	219..242	CDD:320428	13/22 (59%)
		TM helix 5	265..294	CDD:320428	17/28 (61%)
		7tm_GPCRs	<707..783	CDD:475119	49/75 (65%)
		TM helix 6	716..738	CDD:410628	16/21 (76%)
		TM helix 7	751..776	CDD:410628	17/24 (71%)
chrm5b	XP_068071586.2	None