DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment mAChR-A and CHRM4

DIOPT Version :9

Sequence 1:	NP_523844.2	Gene:	mAChR-A / 37892	FlyBaseID:	FBgn0000037	Length:	805	Species:	Drosophila melanogaster
Sequence 2:	NP_000732.2	Gene:	CHRM4 / 1132	HGNCID:	1953	Length:	479	Species:	Homo sapiens

Alignment Length:	726	Identity:	223/726 - (30%)
Similarity:	305/726 - (42%)	Gaps:	260/726 - (35%)

- Green bases have known domain annotations that are detailed below.

  Fly    75 YDPYSGMYGNQTNGTIGFETKGPRYSLASMVVMGFVAAILSTVTVAGNVMVMISFKIDKQLQTIS 139
            :.|.:|..|||:...:...:.. ||....||.:..|...||.|||.||::||:|.|:::||||::
Human     4 FTPVNGSSGNQSVRLVTSSSHN-RYETVEMVFIATVTGSLSLVTVVGNILVMLSIKVNRQLQTVN 67

  Fly   140 NYFLFSLAIADFAIGAISMPLFAVTTILGYWPLGPIVCDTWLALDYLASNASVLNLLIISFDRYF 204
            ||||||||.||..|||.||.|:.|..|.||||||.:|||.||||||:.|||||:|||||||||||
Human    68 NYFLFSLACADLIIGAFSMNLYTVYIIKGYWPLGAVVCDLWLALDYVVSNASVMNLLIISFDRYF 132

  Fly   205 SVTRPLTYRAKRTTNRAAVMIGAAWGISLLLWPPWIYSWPYIEGKRTVPKDECYIQFIETNQYIT 269
            .||:||||.|:|||..|.:||.|||.:|.:||.|.|..|.::.||||||.::|:|||: :|..:|
Human   133 CVTKPLTYPARRTTKMAGLMIAAAWVLSFVLWAPAILFWQFVVGKRTVPDNQCFIQFL-SNPAVT 196

  Fly   270 FGTALAAFYFPVTIMCFLYWRIWRETKKR-QKDLPNLQAGKKDSSKRSNSSDENTVVNHASGGLL 333
            ||||:||||.||.||..||..|...::.| .|..|.   |.|:...::                |
Human   197 FGTAIAAFYLPVVIMTVLYIHISLASRSRVHKHRPE---GPKEKKAKT----------------L 242

  Fly   334 AFAQVGGNDHDTWRRPRSESSPDAESVYMTNMVIDSGYHGMHSRKSSIKSTNTIKKSYTCFGSIK 398
            ||.:                ||                    ..|.|:|
Human   243 AFLK----------------SP--------------------LMKQSVK---------------- 255

  Fly   399 EWCIAWWHSGREDSDDFAYEQEEPSDLGYATPVTIETPLQSSVSRCTSMNVMRDNYSMGGSV--- 460
                      :....:.|.|:.....|..|.|..:..|.:....:.||      |.|..||.
Human   256 ----------KPPPGEAAREELRNGKLEEAPPPALPPPPRPVADKDTS------NESSSGSATQN 304

  Fly   461 SGVRPPSILLSDVSPTP-LPRPPLASISQLQEMSAVTASTTANVNTSGNGNGAINNNNNASHNGN 524
            :..||.:.|.:..:.|| :|.|||    |.:.::..:..:...:.|...||
Human   305 TKERPATELSTTEATTPAMPAPPL----QPRALNPASRWSKIQIVTKQTGN-------------- 351

  Fly   525 GAVNGNGAGNGSGIGLGTTGNATHRDSRTLPVINRINSRSVSQDSVYTILIRLPSDGASSNAANG 589

Human   352 ----------------------------------------------------------------- 351

  Fly   590 GGGGPGAGAAASASLSMQGDCAPSIKMIHEDGPTTTAAAAPLASAAATRRPLPSRDSEFSLPLGR 654
                               :|..:|:::    |.|.|...|.|:.|                  |
Human   352 -------------------ECVTAIEIV----PATPAGMRPAANVA------------------R 375

  Fly   655 RMSHAQHDARLLNAKVIPKQLGKAGGGAAGGGVGGAHALMNARNAAKKKKKSQEKRQESKAAKTL 719
            :.:..                                    |||..:||:  |...:|.|..:|:
Human   376 KFASI------------------------------------ARNQVRKKR--QMAARERKVTRTI 402

  Fly   720 SAILLSFIITWTPYNILVLIKPLTTCSDCIPTELWDFFYALCYINSTINPMCYALCNATFRRTYV 784
            .||||:||:||||||::||:.  |.|..|||..:|...|.|||:||||||.|||||||||::|:.
Human   403 FAILLAFILTWTPYNVMVLVN--TFCQSCIPDTVWSIGYWLCYVNSTINPACYALCNATFKKTFR 465

  Fly   785 RILTCKWHTRN 795
            .:|.|::  ||
Human   466 HLLLCQY--RN 474

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mAChR-A	NP_523844.2	7tm_4	117..>241	CDD:304433	82/123 (67%)
mAChR-A	NP_523844.2	7tm_1	121..>314	CDD:278431	113/193 (59%)
CHRM4	NP_000732.2	7tmA_mAChR_M4	33..464	CDD:341344	210/682 (31%)
		TM helix 1	35..59	CDD:341344	11/23 (48%)
		TM helix 2	68..90	CDD:341344	16/21 (76%)
		TM helix 3	106..128	CDD:341344	17/21 (81%)
		TM helix 4	151..167	CDD:341344	8/15 (53%)
		TM helix 5	193..216	CDD:341344	14/22 (64%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	271..333		20/71 (28%)
		TM helix 6	399..421	CDD:341344	13/21 (62%)
		TM helix 7	432..457	CDD:341344	15/24 (63%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	349	1.000	Domainoid score	I1027
eggNOG	1	0.900	-	-		E1_KOG4220
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	385	1.000	Inparanoid score	I2042
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1245472at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm41771
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR24247
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X324
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	10	9.930