DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment mAChR-A and chrm3

DIOPT Version :9

Sequence 1:	NP_523844.2	Gene:	mAChR-A / 37892	FlyBaseID:	FBgn0000037	Length:	805	Species:	Drosophila melanogaster
Sequence 2:	XP_002935876.2	Gene:	chrm3 / 100493547	XenbaseID:	XB-GENE-940647	Length:	588	Species:	Xenopus tropicalis

Alignment Length:	771	Identity:	252/771 - (32%)
Similarity:	336/771 - (43%)	Gaps:	253/771 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly    57 TLLTALFENLTSTAASGLYDPYSGMYGNQT---------------------------NGT----- 89
            ||||.| .|::|||.:.|   .||...|.|                           ||.
Frog     7 TLLTIL-ANISSTAKTLL---QSGDLYNATEITFWSNDITKFFYNTSDIKDEVNISDNGNYTERD 67

  Fly    90 -IGFETKGPRYSLASMVVMGFVAAILSTVTVAGNVMVMISFKIDKQLQTISNYFLFSLAIADFAI 153
             :|..|....:.:||.      :.|::.|||.||::|:::||::|||:|::||||.|||.||..|
Frog    68 YLGGHTVWQVFLIASF------SGIIALVTVIGNILVILAFKVNKQLKTVNNYFLLSLACADLII 126

  Fly   154 GAISMPLFAVTTILGYWPLGPIVCDTWLALDYLASNASVLNLLIISFDRYFSVTRPLTYRAKRTT 218
            |.|||.||....|:.:|.:|.:.||.||.:||:||||||:|||:||||||||:|||||||.||||
Frog   127 GVISMNLFTTYIIMDHWIMGSLACDLWLMIDYVASNASVMNLLVISFDRYFSITRPLTYRTKRTT 191

  Fly   219 NRAAVMIGAAWGISLLLWPPWIYSWPYIEGKRTVPKDECYIQFIETNQYITFGTALAAFYFPVTI 283
            .||.:|||.||.||.:||.|.|..|.|..||||||.|||:|||. |...||||||:||||.||:|
Frog   192 KRAGIMIGLAWIISFILWAPAILFWQYFVGKRTVPSDECFIQFF-TEPIITFGTAIAAFYLPVSI 255

  Fly   284 MCFLYWRIWRETKKRQKDLPNLQAGKKDSSKRSNSSDENTVVNHASGGLLAFAQVGGNDHDTWRR 348
            |..|||||::||:||.|:|..||                     ||||.|   :|.|..|.|
Frog   256 MTILYWRIYKETEKRTKELAGLQ---------------------ASGGKL---EVPGLSHRT--- 293

  Fly   349 PRSESSPDAESVYMTNMVIDSGYHGMHSRKSSIKSTNTIK-KSYTCFGSIKEWCIAWWHSGREDS 412
                                    |.....|:.|....|| .|.|.:    .||...:......|
Frog   294 ------------------------GNAKGNSNYKIQRNIKFSSRTRY----SWCTFRFKPKMCKS 330

  Fly   413 DDFAYEQEEPSDLGYATPVTIETPLQSSVSRCTSMNVMRDNYSMGGSVSGVRPPSILLSDVSPTP 477
            |....||::.|
Frog   331 DKKQIEQDQSS------------------------------------------------------ 341

  Fly   478 LPRPPLASISQLQEMSAVTASTTANVNTSGNGNGAINNNNNASHNGNGAVNGNGAGNGSGIGLGT 542
                                      :.|.|.|.|     |||                   |..
Frog   342 --------------------------SESWNHNDA-----NAS-------------------LDN 356

  Fly   543 TGNATHRDSRTLPVINRINSRSVSQDSVYTILIRLPSDGASSNAANGGGGGPGAGAAASASLSMQ 607
            :|.:...|:...|            .::|:|:::|                ||..:..|.:.|..
Frog   357 SGTSDEEDAPENP------------RAIYSIVLKL----------------PGHNSVLSKTKSQS 393

  Fly   608 GDCAPSIKMIHEDGPTTTAAAAPLASAAATRRPLPSRDSEFSLPLGRRMSHAQHDARLLNAKVIP 672
            .:...:.|.:.:.||..........:   ::|.|.::|...|:.|..:..|:.:.:....|. :|
Frog   394 SNQGNTSKGMQKSGPQPKELRFKNLN---SQRNLQNKDESISVSLSPQTDHSMNTSSKTTAP-LP 454

  Fly   673 ---KQLGKAGGGAAGGGVGGAHALMNARNAAKKKKKSQEKRQESKAAKTLSAILLSFIITWTPYN 734
               |::..|...||           ..||...|:|: ....:|.|||:|||||||:|||||.|||
Frog   455 ISFKEIALAKRFAA-----------KTRNQITKRKR-MSLIKEKKAAQTLSAILLAFIITWAPYN 507

  Fly   735 ILVLIKPLTTCSDCIPTELWDFFYALCYINSTINPMCYALCNATFRRTYVRILTCK 790
            |:||:.  |.|..|||...|:..|.||||||||||:||||||.|||.|:..:|.|:
Frog   508 IMVLVN--TFCESCIPRIYWNLGYWLCYINSTINPICYALCNKTFRTTFKILLLCQ 561

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mAChR-A	NP_523844.2	7tm_4	117..>241	CDD:304433	77/123 (63%)
mAChR-A	NP_523844.2	7tm_1	121..>314	CDD:278431	117/192 (61%)
chrm3	XP_002935876.2	7tmA_mAChR_M3	75..>279	CDD:320426	123/231 (53%)
		TM helix 1	80..104	CDD:320426	10/29 (34%)
		TM helix 2	113..135	CDD:320426	15/21 (71%)
		TM helix 3	151..173	CDD:320426	15/21 (71%)
		TM helix 4	196..212	CDD:320426	9/15 (60%)
		TM helix 5	238..261	CDD:320426	15/22 (68%)
		7tm_GPCRs	<483..563	CDD:421689	51/81 (63%)
		TM helix 6	486..513	CDD:410628	21/26 (81%)
		TM helix 7	522..547	CDD:410628	16/24 (67%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	352	1.000	Domainoid score	I1000
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	393	1.000	Inparanoid score	I1932
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1245472at2759
OrthoFinder	1	1.000	-	-		FOG0001308
OrthoInspector	1	1.000	-	-		otm48994
Panther	1	1.100	-	-	LDO	PTHR24247
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X324
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	10	10.070