Protein Alignment mAChR-A and chrm4a

DIOPT Version :10

• Sequence 1: NP_523844.2
• Gene: mAChR-A / 37892
• FlyBaseID: FBgn0000037
• Length: 805
• Species: Drosophila melanogaster
• Sequence 2: XP_001922442.1
• Gene: chrm4a / 100150701
• ZFINID: ZDB-GENE-090410-6
• Length: 513
• Species: Danio rerio
• Alignment Length: 745
• Identity: 225/745 - (30%)
• Similarity: 313/745 - (42%)
• Gaps: 257/745 - (34%)

  Fly    65 NLT-STAASGLYD-PYSGMYGN------------QTNGT---IGFETKGPRYSLASMVVMGFVAA 112
            |:| ||.|.||.. .::|...|            ..||:   :..:.:.| |....||.:..|..
Zfish     2 NVTNSTGAEGLAPWNFNGSLSNVSSDANFSCDSGSANGSCAAVEGDGESP-YKTVEMVFIALVTG 65

  Fly   113 ILSTVTVAGNVMVMISFKIDKQLQTISNYFLFSLAIADFAIGAISMPLFAVTTILGYWPLGPIVC 177
            .||.|||.||::||:|.|:::.|||::||||||||.||..||..||.|:.|..|.|||||||:||
Zfish    66 SLSFVTVVGNILVMLSIKVNRHLQTVNNYFLFSLACADLIIGVFSMNLYTVYIIKGYWPLGPVVC 130

  Fly   178 DTWLALDYLASNASVLNLLIISFDRYFSVTRPLTYRAKRTTNRAAVMIGAAWGISLLLWPPWIYS 242
            |.||||||:.|||||:|||||||||||.||:||:|..:|||..|.:||.:||.:|.:||.|.|..
Zfish   131 DLWLALDYVVSNASVMNLLIISFDRYFCVTKPLSYPTRRTTKMAGLMIASAWILSFILWAPAILF 195

  Fly   243 WPYIEGKRTVPKDECYIQFIETNQYITFGTALAAFYFPVTIMCFLYWRIWRETKKR-QKDLPNLQ 306
            |.:|.|.|||...||||||: :|..:|||||:||||.||.||..||..|...::.| .|..|..:
Zfish   196 WQFIVGARTVAPGECYIQFL-SNPAVTFGTAIAAFYLPVVIMTVLYVHISLASRSRVSKQKPEAK 259

  Fly   307 AGKKDSSKRSNSSDENTVVNHASGGLLAFAQVGGNDHDTWRRPRSESSPDAESVYMTNMVIDSGY 371
            ..||...               |.|||       ..|...:...::|.|
Zfish   260 KEKKAQK---------------SSGLL-------KSHILKQNNNNQSPP---------------- 286

  Fly   372 HGMHSRKSSIKSTNTIKKSYTCFGSIKEWCIAWWHSGREDSDDFAYEQEEPSDLGYATPVTIETP 436
                  |.|:.:.:|:.....  |.::|..:    |.:.||...|.|:|..:|...|: :..:.|
Zfish   287 ------KPSLDTCSTVDTMKN--GKLEESVV----SAKADSCVQAEEKESSNDSSTAS-IAPKEP 338

  Fly   437 LQSSVSRCTSMNVMRDNYSMGGSVSGVRPPSILLSDVSPTPLPRPPLASISQLQEMSAVTASTTA 501
            .:.:.|..||.                       :.::|.|...|.|...|:..::..||..
Zfish   339 KERANSEVTSD-----------------------AGLTPAPAAAPKLNPQSKWSKIKIVTKQ--- 377

  Fly   502 NVNTSGNGNGAINNNNNASHNGNGAVNGNGAGNGSGIGLGTTGNATHRDSRTLPVINRINSRSVS 566
                                          ||:
Zfish   378 ------------------------------AGD-------------------------------- 380

  Fly   567 QDSVYTILIRLPSDGASSNAANGGGGGPGAGAAASASLSMQGDCAPSIKMIHEDGPTTTAAAAPL 631
                                                      :|..:|:::            |.
Zfish   381 ------------------------------------------ECITAIEIV------------PP 391

  Fly   632 ASAAATRRPLPSRDSEFSLPLGRRMSHAQHDARLLNAKVIPKQLGKAGGGAAGGGVGGAHALMNA 696
            .|...:|          |:|:.|..:.|:..|.:..::|
Zfish   392 NSTGESR----------SIPVSRPRTVARKFASIARSQV-------------------------- 420

  Fly   697 RNAAKKKKKSQEKRQESKAAKTLSAILLSFIITWTPYNILVLIKPLTTCSDCIPTELWDFFYALC 761
                  |:|.|...:|.|..||:.||||:|||||||||::|||.  |.|..|:|..:|...|.||
Zfish   421 ------KRKRQMAAREKKVTKTIFAILLAFIITWTPYNVMVLIS--TFCQSCVPDTVWAIGYWLC 477

  Fly   762 YINSTINPMCYALCNATFRRTYVRILTCKW 791
            |:||||||.|||||||||::|:..:|.|::
Zfish   478 YVNSTINPACYALCNATFKKTFKNLLMCQY 507

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
mAChR-A	NP_523844.2	7tmA_mAChR_DM1-like	105..>300	CDD:320428	113/195 (58%)
		TM helix 1	106..132	CDD:320428	12/25 (48%)
		TM helix 2	139..165	CDD:320428	16/25 (64%)
		TM helix 3	177..207	CDD:320428	24/29 (83%)
		TM helix 4	219..242	CDD:320428	10/22 (45%)
		TM helix 5	265..294	CDD:320428	17/28 (61%)
		7tm_GPCRs	<707..783	CDD:475119	45/75 (60%)
		TM helix 6	716..738	CDD:410628	15/21 (71%)
		TM helix 7	751..776	CDD:410628	15/24 (63%)
chrm4a	XP_001922442.1	7tmA_mAChR_M4	58..499	CDD:341344	208/678 (31%)
		TM helix 1	59..85	CDD:341344	12/25 (48%)
		TM helix 2	92..118	CDD:341344	16/25 (64%)
		TM helix 3	130..160	CDD:341344	24/29 (83%)
		TM helix 4	172..195	CDD:341344	10/22 (45%)
		TM helix 5	217..246	CDD:341344	17/28 (61%)
		TM helix 6	428..458	CDD:341344	19/29 (66%)
		TM helix 7	467..492	CDD:341344	15/24 (63%)