DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment mAChR-A and chrm4

DIOPT Version :9

Sequence 1:	NP_523844.2	Gene:	mAChR-A / 37892	FlyBaseID:	FBgn0000037	Length:	805	Species:	Drosophila melanogaster
Sequence 2:	XP_031755648.1	Gene:	chrm4 / 100127705	XenbaseID:	XB-GENE-5872073	Length:	505	Species:	Xenopus tropicalis

Alignment Length:	740	Identity:	232/740 - (31%)
Similarity:	305/740 - (41%)	Gaps:	265/740 - (35%)

- Green bases have known domain annotations that are detailed below.

  Fly    64 ENLTSTAASGLYDPYSGMYGNQTNGTIGFETKGP-RYSLASMVVMGFVAAILSTVTVAGNVMVMI 127
            ||:|                |.||.|:...|..| .|....|:.:..|...||.|||.||::||:
Frog    29 ENMT----------------NATNQTLDKTTMAPGTYQTMEMIFIATVTGSLSLVTVVGNILVML 77

  Fly   128 SFKIDKQLQTISNYFLFSLAIADFAIGAISMPLFAVTTILGYWPLGPIVCDTWLALDYLASNASV 192
            |.|:::||||::||||||||.||..||..||.|:::..|.|||||||||||.||||||:.|||||
Frog    78 SIKVNRQLQTVNNYFLFSLACADLIIGVFSMNLYSLYIIKGYWPLGPIVCDLWLALDYVVSNASV 142

  Fly   193 LNLLIISFDRYFSVTRPLTYRAKRTTNRAAVMIGAAWGISLLLWPPWIYSWPYIEGKRTVPKDEC 257
            :|||||||||||.||:||||.|:|||..|.:||.|||.:|.:||.|.|..|.:|.|:|||...||
Frog   143 MNLLIISFDRYFCVTKPLTYPARRTTKMAGLMIAAAWLLSFVLWAPAILFWQFIVGERTVASGEC 207

  Fly   258 YIQFIETNQYITFGTALAAFYFPVTIMCFLYWRIWRETKKRQKDLPNLQAGKKDSSKRSNSSDEN 322
            ||||: :|..:|||||:||||.||.||..||..|...::.|
Frog   208 YIQFL-SNPVVTFGTAIAAFYLPVVIMTILYIHISLASRSR------------------------ 247

  Fly   323 TVVNHASGGLLAFAQVGGNDHDTWRRPRSESSPDAESVYMTNMVIDSGYHGMHSRKSSIKSTNTI 387
             |..|.:                  ..|.|......|:                :...||.|..:
Frog   248 -VRRHCT------------------ETRQEKKKPISSM----------------KSPLIKQTKNL 277

  Fly   388 KKSYTCFGSIKEWCIAWWHSGREDSDDFAYEQEEPSDLGYATPVTIETPLQSSVSRCTSMNVMRD 452
            .|                    :|..|.|.::|
Frog   278 PK--------------------QDPGDEAVKKE-------------------------------- 290

  Fly   453 NYSMGGSVSGVRPPSILLSDVSPTPLPRPPLASISQLQEMSAVTASTTANVNTSGNGNGAINNNN 517
                    :|||...|                      |.|.....|.....||       |.::
Frog   291 --------NGVRNGKI----------------------EKSMTNLQTAEEKETS-------NESS 318

  Fly   518 NASHNGNGAVNGNGAGNGSGIGLGTTGNATHRDSRTLPVINRINSRSV-SQDSVYTILIRLPSDG 581
            :||.:.|.......:...||:.|..|.|..       |:.:::|..|. |:..:.|
Frog   319 SASLSHNPPEKQPVSDASSGVVLAPTQNMP-------PLPSKVNVASKWSKIKIVT--------- 367

  Fly   582 ASSNAANGGGGGPGAGAAASASLSMQGDCAPSIKMIHEDGPTTTAAAAPLASAAATRRPLPSRDS 646
              ....|                    :|..:|:::.|       .|.||.. .|..||
Frog   368 --KQTGN--------------------ECVTAIEIVPE-------CAIPLPQ-QANNRP------ 396

  Fly   647 EFSLPLGRRMSHAQHDARLLNAKVIPKQLGKAGGGAAGGGVGGAHALMNARNAAKKKKKSQEKRQ 711
                                                  |.|....|.: |||..:||:  |...:
Frog   397 --------------------------------------GNVARKFASI-ARNQVRKKR--QMAAR 420

  Fly   712 ESKAAKTLSAILLSFIITWTPYNILVLIKPLTTCSDCIPTELWDFFYALCYINSTINPMCYALCN 776
            |.|..:|:.||||:|||||||||::|||.  |.|..|||..:|...|.|||:||||||.||||||
Frog   421 EKKVTRTIFAILLAFIITWTPYNVMVLIN--TFCQTCIPETVWYIGYWLCYVNSTINPACYALCN 483

  Fly   777 ATFRRTYVRILTCKWHTRNREGMVR 801
            |||::|:..:|.|::.:   .||.|
Frog   484 ATFKKTFKHLLMCQYKS---IGMAR 505

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mAChR-A	NP_523844.2	7tm_4	117..>241	CDD:304433	82/123 (67%)
mAChR-A	NP_523844.2	7tm_1	121..>314	CDD:278431	110/192 (57%)
chrm4	XP_031755648.1	7tmA_mAChR_M4	55..490	CDD:341344	215/678 (32%)
		TM helix 1	57..81	CDD:341344	11/23 (48%)
		TM helix 2	90..112	CDD:341344	15/21 (71%)
		TM helix 3	128..150	CDD:341344	17/21 (81%)
		TM helix 4	173..189	CDD:341344	8/15 (53%)
		TM helix 5	215..238	CDD:341344	14/22 (64%)
		TM helix 6	425..447	CDD:341344	14/21 (67%)
		TM helix 7	458..483	CDD:341344	15/24 (63%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1245472at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm48994
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X324
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	3.010