DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Alas and hem1

DIOPT Version :9

Sequence 1:	NP_477281.1	Gene:	Alas / 37815	FlyBaseID:	FBgn0020764	Length:	539	Species:	Drosophila melanogaster
Sequence 2:	NP_594388.1	Gene:	hem1 / 2542028	PomBaseID:	SPAC2F3.09	Length:	558	Species:	Schizosaccharomyces pombe

Alignment Length:	528	Identity:	224/528 - (42%)
Similarity:	302/528 - (57%)	Gaps:	42/528 - (7%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 CPFLNRFTASFIRNYAETLCQSYGSHCPVVGKTL-------------VSGEKKLSLVAASVTRSH 54
            |||:::...|.:|..|.......|:.||||...|             ...:.|...:..|:...|
pombe    12 CPFVSKADPSALRRMAGAGLIRAGARCPVVRHALPVAAATGADVSRGFKSDSKQMAMEPSLDEIH 76

  Fly    55 ------STGANA--HANA-GGPATANATAPVSAPASADPGKAS--------AKETFPYERFFNEQ 102
                  :||:..  ||:| ...|.|..|.||:.........||        ....|.|:.|:.|:
pombe    77 LKAGVVNTGSRTCRHADAVKAAAEAATTTPVTKKHQMPKHYASDLNGVGPATTPRFDYDTFYREE 141

  Fly   103 IMKKKRDHSYRVFKKVNRLAGDGLFPHALEYSERTEKPITVWCSNDYLGMSAHPGVKRAVQDALN 167
            :.||.||.|||.|..:||||.:....|..:.:.|.|    ||||||||.|..|..::.|:...:.
pombe   142 LDKKHRDKSYRYFNNINRLAKEYPLAHLADPNTRVE----VWCSNDYLNMGGHKKIREAMHQCIE 202

  Fly   168 RHGSGAGGTRNISGNSLHHERLESKLAELHQKEAALLFTSCFVANDSTLFTLAKLLPGCEIFSDA 232
            .:|.||||||||:|::.|..|||..||:||||.|||:|.||:||||:||.||.:.||.|...||.
pombe   203 TYGGGAGGTRNIAGHNQHAVRLEKSLADLHQKPAALVFGSCYVANDATLSTLGRKLPNCIFLSDE 267

  Fly   233 GNHASMIMGIRNSGVPKHIFRHNDVDHLHQLLKQTDKSVPKIVAFETVHSMTGAICPLEELLDVA 297
            .||||||.|||||...|.||:|||:..|...|.....:.|||:|||:|:||:|.:.|:.|:.|:|
pombe   268 MNHASMINGIRNSRCEKIIFKHNDLVDLEAKLASLPLNRPKIIAFESVYSMSGNVAPISEICDLA 332

  Fly   298 HEHGAITFIDEVHAVGLYGDHGAGVGER-DGVLHKMDIISGTLGKAFGNIGGYIAGTHNLVDMIR 361
            .::|||||:|||||||:||..||||.|. .|:|.::|||:|||.|::|.:|||||.:..||||||
pombe   333 KKYGAITFLDEVHAVGMYGPRGAGVAEETPGLLSRVDIITGTLAKSYGCVGGYIAASSTLVDMIR 397

  Fly   362 SYAAGFIFTTSLPPTVLCGALEAVNIL--ASEEGRQLRHLHQRNVSYLKSLLKREGFPVEETPSH 424
            |.|.||||||||||.|:.|||.||..|  ::.|..|.|...:|    :|..|...|.||....:|
pombe   398 SLAPGFIFTTSLPPHVMVGALTAVEHLKVSNVEREQQRSAVRR----VKQSLSEIGIPVLSNDTH 458

  Fly   425 IIPIKIGDPLKSSQISNVLIEQFGHYLQSINYPTVARGQEKLRLAPTPFHTFE-MMNALVTDLKK 488
            |:|..:||...:...|:.|:.....|:||||:|||:.|.|:||:.|||.|..| .:.:|...:..
pombe   459 IVPAMVGDAHLAKLASDSLLHDHNIYVQSINFPTVSVGTERLRITPTPAHNTEHYVQSLTNAMND 523

  Fly   489 VWEMVDLS 496
            ||...:::
pombe   524 VWSKFNIN 531

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Alas	NP_477281.1	AAT_I	93..495	CDD:302748	197/405 (49%)
Alas	NP_477281.1	BioF	95..490	CDD:223234	194/398 (49%)
hem1	NP_594388.1	Preseq_ALAS	11..>53	CDD:286162	12/40 (30%)
		BioF	132..525	CDD:223234	195/400 (49%)
		PRK09064	133..532	CDD:236370	196/407 (48%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	333	1.000	Domainoid score	I201
eggNOG	1	0.900	-	-		E1_COG0156
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H17
Inparanoid	1	1.050	378	1.000	Inparanoid score	I422
OMA	1	1.010	-	-		QHG58214
OrthoFinder	1	1.000	-	-		FOG0000902
OrthoInspector	1	1.000	-	-		oto101167
orthoMCL	1	0.900	-	-		OOG6_100359
Panther	1	1.100	-	-	LDO	PTHR13693
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R554
SonicParanoid	1	1.000	-	-		X1924
TreeFam	1	0.960	-	-
	14	13.860