DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment kcc and Slc12a4

DIOPT Version :9

Sequence 1:	NP_001286795.1	Gene:	kcc / 37800	FlyBaseID:	FBgn0261794	Length:	1077	Species:	Drosophila melanogaster
Sequence 2:	NP_001240733.1	Gene:	Slc12a4 / 20498	MGIID:	1309465	Length:	1087	Species:	Mus musculus

Alignment Length:	1119	Identity:	610/1119 - (54%)
Similarity:	789/1119 - (70%)	Gaps:	93/1119 - (8%)

- Green bases have known domain annotations that are detailed below.

  Fly     7 KDDSDEMEIIPSEHNGTVHETKNEDSGDIHRAEENQ-----------KSSIDPNLYLYDDDLETR 60
            :.|.|.:|.:.....|...|.::.|........||.           ....|.||.|::::|:.|
Mouse    14 RGDYDNLEGLSWVDYGERAEREDSDGEGQGNHRENSPFLCPLDASRGNDYYDRNLALFEEELDIR 78

  Fly    61 PHISTFISSIANYENTIPAATDPD--------AKPAAPSARMGTLIGVFLPCIQNIFGVILFIRL 117
            |.:|:.:..:.:|.|....|.:.:        .:.||.:..||||:||:|||:|||||||||:||
Mouse    79 PKVSSLLGKLVSYTNLTQGAKEHEEAESGEGGRRRAAKAPSMGTLMGVYLPCLQNIFGVILFLRL 143

  Fly   118 TWVVGTAGAVCGFLIVLTCCCVTMLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGMLFY 182
            ||:|||||.:...||||.|||.|:|||||||||||||||||||||||||||||||||||||:.||
Mouse   144 TWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFY 208

  Fly   183 TGTTLAAAMYIVGAVEIVLTYMAPWASIF-GDFTKD-ADAMYNNFRVYGTLLLIFMGLIVFLGVK 245
            .|||.||||||:||:||:|||:||.|:|| ...|.| :.|..||.|||||:.|..|.|:||:|||
Mouse   209 LGTTFAAAMYILGAIEILLTYIAPPAAIFYPSGTHDMSSATLNNMRVYGTIFLTLMTLVVFVGVK 273

  Fly   246 FVNKFATVALACVILSIIAVYVGIFDNIHGNEKLYMCVLGKRLLKDIPLENCTKE--------DS 302
            :|||||::.|||||:||:::|.|...:|.......:|:||.|.|.....:.|.|.        .:
Mouse   274 YVNKFASLFLACVIISILSIYAGGIKSIFDPPVFPVCMLGNRTLSRDQFDICAKTVVVDNETVAT 338

  Fly   303 FLRDIYC--PD---GKCEEYYLANNVTKVKGIKGLASGVFYDNIFPSFLEKGQFISYGKSAIDIE 362
            .|...:|  |:   ..|:.|:|.||||::.||.|.|:||..:|::.::||||:.:.  |..:...
Mouse   339 RLWTFFCHSPNLTADSCDPYFLLNNVTEIPGIPGAAAGVLQENLWSAYLEKGEVVE--KHGLPST 401

  Fly   363 NTSG--ESYN-QIMADITTSFTLLIGIFFPSVTGIMAGSNRSGDLADAQKSIPIGTICAILTTST 424
            :|.|  ||.: .::|||.||||:|:||||||||||||||||||||.|||||||:|||.||:|||.
Mouse   402 DTLGLKESLSLYVVADIATSFTVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTTSL 466

  Fly   425 VYLSSVMFFAGTVDNLLLRDKFGQSIGGKLVVANIAWPNQWVILIGSFLSTLGAGLQSLTGAPRL 489
            ||.|||:.|...::.::||||:|..:...|||..:|||:.|||::|||.||.|||||||||||||
Mouse   467 VYFSSVILFGACIEGVVLRDKYGDGVSRNLVVGTLAWPSPWVIVVGSFFSTCGAGLQSLTGAPRL 531

  Fly   490 LQAIARDEIIPFLAPFAKSSKRGEPTRALLLTIVICQCGILLGNVDLLAPLLSMFFLMCYGFVNL 554
            |||||:|.|||||..|......||||.|||||.:|.:.|||:.::|::||:|||||||||.||||
Mouse   532 LQAIAKDNIIPFLRVFGHGKANGEPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNL 596

  Fly   555 ACAVQTLLRTPNWRPRFKFYHWSLSLIGLTLCISVMIMTSWYFALIAMGMAIIIYKYIEYRGAEK 619
            ||||||||||||||||||:|||:||.:|::||:::|.::|||:||:||.:|.:|||||||:||||
Mouse   597 ACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLALMFVSSWYYALVAMLIAGMIYKYIEYQGAEK 661

  Fly   620 EWGDGIRGMALTAARYSLLRLEEGPPHTKNWRPQILVLSKLNDNLLPKYRKIFSFATQLKAGKGL 684
            ||||||||::|:||||:|||||||||||||||||:|||.||:::|..||.::.:||:||||||||
Mouse   662 EWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKYPRLLTFASQLKAGKGL 726

  Fly   685 TICVSVIKGDHTKITNKAVDAKATLRKYMTDEKVKGFCDVLVAQQIGEGLSSVIQTIGLGGMKPN 749
            ||..|||:|...:...:|..|:.|::..|..|||||||.|:||.::.|||:.:||:.|||||:.|
Mouse   727 TIVGSVIQGSFLESYGEAQAAEQTIKNMMDIEKVKGFCQVVVASKVREGLAHLIQSCGLGGMRHN 791

  Fly   750 TVIIGWPYSWRQ-EGRNSWKTFIQTVRTVAACHMALMVPKGINFYPESNHK--IGGNIDIWWIVH 811
            :|::||||.||| |...:|||||.|||...|.|:||:|||.|.||| |||:  :.|:||:|||||
Mouse   792 SVVLGWPYGWRQSEDPRAWKTFIDTVRCTTAAHLALLVPKNIAFYP-SNHERYLDGHIDVWWIVH 855

  Fly   812 DGGLLMLLPFLLKQHRTWRNCKLRIFTVAQIEDNSIQMKKDLKTFLYHLRIEADVEVVEMNNSDI 876
            |||:||||||||:||:.|:.|::|||||||::||||||||||..||||||:||:||||||:||||
Mouse   856 DGGMLMLLPFLLRQHKVWKKCRMRIFTVAQMDDNSIQMKKDLAIFLYHLRLEAEVEVVEMHNSDI 920

  Fly   877 SAYTYERTLMMEQRNQMLRALGLNKKENSKVVQTIMDYKETPSDNKMSLVQTIVDHHYDATKTAS 941
            ||||||||||||||:||||.:.|.|.|..:..|.:.|                   .:.|.:..|
Mouse   921 SAYTYERTLMMEQRSQMLRQMRLTKTERDREAQLVKD-------------------RHSALRLES 966

  Fly   942 KVRFADPTIEETQHHDSQNDEKRNSIDLDGPENADTPETTSNKDE------STEKADGDFKS--S 998
                            ..:||:..|:     ..||..:.|..:|:      ....|..:|:.  .
Mouse   967 ----------------LYSDEEEESV-----AGADKIQMTWTRDKYMAEPWDPSHAPDNFRELVH 1010

  Fly   999 VKPDEFNVRRMHTAIKLNEVIVEKSQDAQLVIMNLPGPPREVRAERERNYMEFLEVLTEGLEKVL 1063
            :|||:.||||||||:|||||||.:|.||:||::|:||||:  .:|.:.||||||||||||||:||
Mouse  1011 IKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPK--NSEGDENYMEFLEVLTEGLERVL 1073

  Fly  1064 MVRGGGREVITIYS 1077
            :|||||||||||||
Mouse  1074 LVRGGGREVITIYS 1087

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
kcc	NP_001286795.1	2a30	49..1077	CDD:273347	599/1064 (56%)
kcc	NP_001286795.1	SLC12	674..1077	CDD:281515	220/413 (53%)
Slc12a4	NP_001240733.1	2a30	44..1087	CDD:273347	602/1087 (55%)
		SLC5-6-like_sbd	144..>305	CDD:294310	108/160 (68%)
		SLC12	711..1087	CDD:281515	220/418 (53%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167848619
Domainoid	1	1.000	426	1.000	Domainoid score	I607
eggNOG	1	0.900	-	-		E2759_KOG2082
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H21056
Inparanoid	1	1.050	1163	1.000	Inparanoid score	I205
Isobase	1	0.950	-	0	Normalized mean entropy	S2108
OMA	1	1.010	-	-		QHG57166
OrthoDB	1	1.010	-	-		D110005at33208
OrthoFinder	1	1.000	-	-		FOG0000627
OrthoInspector	1	1.000	-	-		otm43147
orthoMCL	1	0.900	-	-		OOG6_101034
Panther	1	1.100	-	-	LDO	PTHR11827
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3041
SonicParanoid	1	1.000	-	-		X451
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	18	17.750