DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pde8 and PDE5A

DIOPT Version :9

Sequence 1:	NP_001163275.2	Gene:	Pde8 / 37741	FlyBaseID:	FBgn0266377	Length:	1050	Species:	Drosophila melanogaster
Sequence 2:	NP_001074.2	Gene:	PDE5A / 8654	HGNCID:	8784	Length:	875	Species:	Homo sapiens

Alignment Length:	931	Identity:	200/931 - (21%)
Similarity:	336/931 - (36%)	Gaps:	265/931 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly   222 KGERNHAE--EEPL--TPGHDHEDSVVLRRKVNCLERKAHSLYERRL-PRVPKLHLLVAGKKNPP 281
            :|.|.|.|  ..||  :|..|:.......||::..|      ::|.| |.|.|
Human    70 EGIRGHTESCSCPLQQSPRADNSAPGTPTRKISASE------FDRPLRPIVVK------------ 116

  Fly   282 TEEEEFRTFQRNLMDLKYPTVLPPNPP---------LKALLVFHKSDSICEAITAACQR-----H 332
             :.|...:|   |.|.:....:|..||         ...||...|..|....:||.|.:     |
Human   117 -DSEGTVSF---LSDSEKKEQMPLTPPRFDHDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIH 177

  Fly   333 QL--------------------------DVTLVKSKEEALDTLQK--------SYATAQCYHLII 363
            .|                          ||....:.||..:...:        .:..|....|.|
Human   178 GLISADRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEVSNNCIRLEWNKGIVGHVAALGEPLNI 242

  Fly   364 IDARSSKNLDAEHIARTIRHTHGHHLTTII----------------AVCKKS-----FFEKDDVL 407
            .||......:||     :....|:...:|:                |:.|||     |.|||:..
Human   243 KDAYEDPRFNAE-----VDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKD 302

  Fly   408 IALLDAGVNRCVAETTNLAMCSVELKQILHSIIRPHNVMSTQQALYTALHRLKEVVLITDDLLRI 472
            .|             ..||.|.:.|          ||....:.:|   |...:..||:  ||..:
Human   303 FA-------------AYLAFCGIVL----------HNAQLYETSL---LENKRNQVLL--DLASL 339

  Fly   473 QY-ANRATERLLNMRLDEIIS-KQLE--DIFVSD-------LSTISEQCKNIKEFDGILTVRRKS 526
            .: ..::.|.:|......||| .|::  .||:.|       .|....:|:.:::....||....:
Human   340 IFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKSSDTLTREHDA 404

  Fly   527 QEGIPMHVRVVPVACIGSAPTHLIFNFDVPGGQMDFIATLPQPKEAP-----RGSLHSVRRCSFD 586
            .:...|:.:.|         .:.:...::|....|        |..|     .|:::  ::|
Human   405 NKINYMYAQYV---------KNTMEPLNIPDVSKD--------KRFPWTTENTGNVN--QQC--- 447

  Fly   587 VRSIASDGLRRTSLAKLTSLPLEAPITKIINLLSQVQENCSADEARLIDKVLEFLKRE------- 644
            :||:....::.....|         :..:..|:::::||..        ||..|.:.:
Human   448 IRSLLCTPIKNGKKNK---------VIGVCQLVNKMEENTG--------KVKPFNRNDEQFLEAF 495

  Fly   645 ------GLYSPQMKEIRTDDPIATDLIGALLTGPSVYSSRRSSNDSIIRTGSSTRTAAIVPAKMK 703
                  |:.:.||.| ..:..:|..::..     .|.|...|:.:...|...|. .||:||:..
Human   496 VIFCGLGIQNTQMYE-AVERAMAKQMVTL-----EVLSYHASAAEEETRELQSL-AAAVVPSAQ- 552

  Fly   704 SNPIIMELLDESLSWDFDIFKLEEITDYHPLLYLGMEMFRRFDVFATLNIDEN-------VCKAW 761
                .:::.|.|.| ||::..||.     .|..:.|        |..||:.:|       :|: |
Human   553 ----TLKITDFSFS-DFELSDLET-----ALCTIRM--------FTDLNLVQNFQMKHEVLCR-W 598

  Fly   762 LAVIEAHYRKSNTYHNSTHAADVMQA------TGAFITQLTNKDMLVMDRMEEATALIAAAAHDV 820
            :..::.:|||:..|||..||.:..|.      .|....:||:.::|.:        ||||.:||:
Human   599 ILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILAL--------LIAALSHDL 655

  Fly   821 DHPGRSSAFLCNSNDALAVLYNDLTVLENHHAAITFK---LTLGDDKINIFKNLDKETYKSARST 882
            ||.|.:::::..|...||.||.. :::|:||    |.   :.|......|...|..|.||:....
Human   656 DHRGVNNSYIQRSEHPLAQLYCH-SIMEHHH----FDQCLMILNSPGNQILSGLSIEEYKTTLKI 715

  Fly   883 IIDMILATEMTRHFEHLAKFVSVFGGEEPRDHNPQTDEETSILMRRMLIKVADVSNPARPMQFCI 947
            |...||||::..:.:...:|..:....:....:|...|    |...||:...|:|...:|.....
Human   716 IKQAILATDLALYIKRRGEFFELIRKNQFNLEDPHQKE----LFLAMLMTACDLSAITKPWPIQQ 776

  Fly   948 EWARRIAEEYFMQTD-EEKQRHLPIVMPMFDRATCSIPKSQIGFIEYI----IQDMMHAWESFID 1007
            ..|..:|.|:|.|.| |.|:.::.....|.......||..|:|||:.|    .:.:.|..|   |
Human   777 RIAELVATEFFDQGDRERKELNIEPTDLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSE---D 838

  Fly  1008 MPQLITYMQINYSQWKKYDEQ 1028
            ...|:...:.|..:|:...||
Human   839 CFPLLDGCRKNRQKWQALAEQ 859

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pde8	NP_001163275.2	PAS	459..539	CDD:238075	19/90 (21%)
		PAS_9	463..539	CDD:290162	19/86 (22%)
		PDEase_I	775..1016	CDD:278654	68/254 (27%)
PDE5A	NP_001074.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..29
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	78..102		6/23 (26%)
		GAF	164..321	CDD:214500	33/184 (18%)
		GAF	347..513	CDD:214500	34/205 (17%)
		PDEase_I	612..847	CDD:278654	68/254 (27%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165154168
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D904682at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	3	2.850