DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pde8 and PDE4B

DIOPT Version :9

Sequence 1:	NP_001163275.2	Gene:	Pde8 / 37741	FlyBaseID:	FBgn0266377	Length:	1050	Species:	Drosophila melanogaster
Sequence 2:	NP_001032418.1	Gene:	PDE4B / 5142	HGNCID:	8781	Length:	736	Species:	Homo sapiens

Alignment Length:	619	Identity:	161/619 - (26%)
Similarity:	258/619 - (41%)	Gaps:	181/619 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly   503 LSTISEQCKNIKEFD----GILTVRRKSQEGIPMHVRVVPVACIGSAPTHLIFNFDVPGGQMDFI 563
            ::|:|::|.:::...    ..|..:..|..|:.:|
Human    87 ITTVSQECFDVENGPSPGRSPLDPQASSSAGLVLH------------------------------ 121

  Fly   564 ATLPQPKEAPRGSLHSVRRCSFDVRSIASDGLRRTSLAKLTSLPLE--------APITKIINLLS 620
            ||.|.         ||.||.||..||.:...|...::::.:|||.|        .|..:::..|.
Human   122 ATFPG---------HSQRRESFLYRSDSDYDLSPKAMSRNSSLPSEQHGDDLIVTPFAQVLASLR 177

  Fly   621 QVQEN----------------------------------------------C-----------SA 628
            .|:.|                                              |           |.
Human   178 SVRNNFTILTNLHGTSNKRSPAASQPPVSRVNPQEESYQKLAMETLEELDWCLDQLETIQTYRSV 242

  Fly   629 DE------ARLIDKVLEFL---KREG-------------------LYSPQMK--EIRTDDPIATD 663
            .|      .|::::.|..|   .|.|                   :.||..|  |.:....:.|.
Human   243 SEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQNDVEIPSPTQKDREKKKKQQLMTQ 307

  Fly   664 LIGALLTGPSVYSSRRSSNDSIIRTGSSTRTAAIVPAKMKSNPIIMELLDESLSWDFDIFKLEEI 728
            :.|.    ..:..|...:|.||.|.|.:|          ::...:.:.|::...|..:||.:...
Human   308 ISGV----KKLMHSSSLNNTSISRFGVNT----------ENEDHLAKELEDLNKWGLNIFNVAGY 358

  Fly   729 TDYHPLLYLGMEMFRRFDVFATLNIDENVCKAWLAVIEAHYRKSNTYHNSTHAADVMQATGAFIT 793
            :...||..:...:|:..|:..|..|..:....::..:|.||.....||||.|||||.|:|...::
Human   359 SHNRPLTCIMYAIFQERDLLKTFRISSDTFITYMMTLEDHYHSDVAYHNSLHAADVAQSTHVLLS 423

  Fly   794 QLTNKDMLVMDRMEEATALIAAAAHDVDHPGRSSAFLCNSNDALAVLYNDLTVLENHHAAITFKL 858
              |.....|...:|...|:.|||.|||||||.|:.||.|:|..||::|||.:||||||.|:.|||
Human   424 --TPALDAVFTDLEILAAIFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKL 486

  Fly   859 TLGDDKINIFKNLDKETYKSARSTIIDMILATEMTRHFEHLAKFVSVFGGEEPRDHNPQTDEETS 923
             |.::..:||.||.|:..::.|..:|||:|||:|::|...||...::.          :|.:.||
Human   487 -LQEEHCDIFMNLTKKQRQTLRKMVIDMVLATDMSKHMSLLADLKTMV----------ETKKVTS 540

  Fly   924 ------------ILMRRMLIKVADVSNPARPMQFCIEWARRIAEEYFMQTDEEKQRHLPIVMPMF 976
                        |.:.|.::..||:|||.:.::...:|..||.||:|.|.|:|::|.:.| .||.
Human   541 SGVLLLDNYTDRIQVLRNMVHCADLSNPTKSLELYRQWTDRIMEEFFQQGDKERERGMEI-SPMC 604

  Fly   977 DRATCSIPKSQIGFIEYIIQDMMHAWESFIDMPQ 1010
            |:.|.|:.|||:|||:||:..:   ||::.|:.|
Human   605 DKHTASVEKSQVGFIDYIVHPL---WETWADLVQ 635

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pde8	NP_001163275.2	PAS	459..539	CDD:238075	7/39 (18%)
		PAS_9	463..539	CDD:290162	7/39 (18%)
		PDEase_I	775..1016	CDD:278654	100/248 (40%)
PDE4B	NP_001032418.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	51..78
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	189..209		0/19 (0%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	282..301		4/18 (22%)
		PDEase_I	405..646	CDD:278654	100/248 (40%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	685..736
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D904682at2759
OrthoFinder	1	1.000	-	-		FOG0000302
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	3	2.920