Protein Alignment Pde8 and Pde5a

DIOPT Version :9

• Sequence 1: NP_001163275.2
• Gene: Pde8 / 37741
• FlyBaseID: FBgn0266377
• Length: 1050
• Species: Drosophila melanogaster
• Sequence 2: NP_700471.2
• Gene: Pde5a / 242202
• MGIID: 2651499
• Length: 865
• Species: Mus musculus
• Alignment Length: 850
• Identity: 171/850 - (20%)
• Similarity: 302/850 - (35%)
• Gaps: 228/850 - (26%)

  Fly   308 PLKALLVFHKSDSICEAITAACQRHQLDVTLVKSKEEALDTLQKSYATAQCYHLIIIDARSSKNL 372
            ||:.::| ..|:.....::.:.::.|:.:|..:...:..|         ||..|:.:....|.:|
Mouse    99 PLRPIVV-KDSEGTVSFLSDSGKKEQMPLTPPRFDSDEGD---------QCSRLLELVKDISSHL 153

  Fly   373 DAEHIARTI-RHTHG---HHLTTIIAVCKKSFFEKDDVLIA-LLDAGVNRCVAETTNLAMCSVEL 432
            |...:...| .|.||   ....::..||:.|  .||..||: |.|......:.|.:|..:.....
Mouse   154 DVTALCHKIFLHIHGLISADRYSLFLVCEDS--SKDKFLISRLFDVAEGSTLEEASNNCIRLEWN 216

  Fly   433 KQILHSII---RPHNV------------------MSTQQALYTALHRLKEVVLITDDLLRIQYAN 476
            |.|:..:.   .|.|:                  ..||..|...:...:|.|:.....:..:..|
Mouse   217 KGIVGHVAAFGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGN 281

  Fly   477 RATERLLNMRLDEIISKQLEDIFVSDLSTISEQCKNIKEFDGILTVRRKSQEGIPMHVRVVPVAC 541
            ..|           .:::.|..|.:.|:.......|.:.::..|...:::|..:.:
Mouse   282 GGT-----------FTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDL--------- 326

  Fly   542 IGSAPTHLIFNFDVPGGQMDFIATLPQPKEAPRGSLHSVRRCS-FDVRSIASDGLRR-------- 597
                 ..|||.      :...:..:.:...|...|...|::|: |.|.....|...|
Mouse   327 -----ASLIFE------EQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCPDSFSRVFHMECEE 380

  Fly   598 ---------------------TSLAKLTSLPLEAP-ITK-------------------------- 614
                                 ....|.|..||..| :||
Mouse   381 VGKPSDPLTREQDANKINYMYAQYVKNTMEPLNIPDVTKDKRFPWTNENMGHVNTPCIGSLLCTP 445

  Fly   615 -----------IINLLSQVQENCSADEARLIDKVLEFLKRE-------------GLYSPQMKEIR 655
                       :..|:::::||..        |:..|.:.:             |:.:.||.| .
Mouse   446 IKNGKKNKVIGVCQLVNKMEENTG--------KIKAFNQNDEQFLEAFVIFCGLGIQNTQMYE-A 501

  Fly   656 TDDPIATDLIGALLTGPSVYSSRRSSNDSIIRTGSSTRTAAIVPAKMKSNPIIMELLDESLSWDF 720
            .:..:|..::..     .|.|...|:.:...|. ....:||:||:..     .:::.|.|.| ||
Mouse   502 VERAMAKQMVTL-----EVLSYHASAAEEETRE-LQALSAAVVPSAQ-----TLKITDFSFS-DF 554

  Fly   721 DIFKLEEITDYHPLLYLGMEMFRRFDVFATLNIDEN-------VCKAWLAVIEAHYRKSNTYHNS 778
            ::..||.     .|..:.|        |..||:.:|       :|: |:..::.:|||:..|||.
Mouse   555 ELSDLET-----ALCTIRM--------FTDLNLVQNFQMKHEVLCR-WILSVKKNYRKNVAYHNW 605

  Fly   779 THAADVMQATGAFIT--QLTNKDMLVMDRMEEATALIAAAAHDVDHPGRSSAFLCNSNDALAVLY 841
            .||.:..|...|.:.  ::.||    :..:|....||||.:||:||.|.:::::..|...||.||
Mouse   606 RHAFNTAQCMFAALKAGKIQNK----LTDLETLALLIAALSHDLDHRGVNNSYIQRSEHPLAQLY 666

  Fly   842 NDLTVLENHHAAITFK---LTLGDDKINIFKNLDKETYKSARSTIIDMILATEMTRHFEHLAKFV 903
            .. :::|:||    |.   :.|......|...|..:.||:....|...||||::..:.:...:|.
Mouse   667 CH-SIMEHHH----FDQCLMILNSPGNQILSGLSIDEYKTTLKIIKQAILATDLALYIKRRGEFF 726

  Fly   904 SV-----FGGEEPRDHNPQTDEETSILMRRMLIKVADVSNPARPMQFCIEWARRIAEEYFMQTD- 962
            .:     |..|:|....         |...||:...|:|...:|.......|..:|.|:|.|.|
Mouse   727 ELIRKNQFSFEDPLQKE---------LFLAMLMTACDLSAITKPWPIQQRIAELVAAEFFDQGDR 782

  Fly   963 EEKQRHLPIVMPMFDRATCSIPKSQIGFIEYI----IQDMMHAWESFIDMPQLITYMQINYSQWK 1023
            |.|:.::.....|.......||..|:|||:.|    .:.:.|..|   |...|:...:.|..:|:
Mouse   783 ERKELNMEPADLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSE---DCLPLLDGCRKNRQKWQ 844

  Fly  1024 KYDEQ 1028
            ...||
Mouse   845 ALAEQ 849

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pde8	NP_001163275.2	PAS	459..539	CDD:238075	10/79 (13%)
		PAS_9	463..539	CDD:290162	9/75 (12%)
		PDEase_I	775..1016	CDD:278654	68/255 (27%)
Pde5a	NP_700471.2	GAF	154..311	CDD:214500	31/169 (18%)
		GAF	337..503	CDD:214500	24/174 (14%)
		PDEase_I	602..837	CDD:278654	68/255 (27%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167844409
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D904682at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.850