Protein Alignment Pde8 and pde5aa

DIOPT Version :9

• Sequence 1: NP_001163275.2
• Gene: Pde8 / 37741
• FlyBaseID: FBgn0266377
• Length: 1050
• Species: Drosophila melanogaster
• Sequence 2: XP_009305135.1
• Gene: pde5aa / 100334568
• ZFINID: ZDB-GENE-100414-2
• Length: 922
• Species: Danio rerio
• Alignment Length: 1138
• Identity: 225/1138 - (19%)
• Similarity: 359/1138 - (31%)
• Gaps: 396/1138 - (34%)

  Fly    15 KSSAKSSSSSAGRENPSSEPDS-------DTEPYAIAIAA---------------ISDRSGDNRN 57
            :..::.|.|.:|::..|.|..:       :.|..||..||               :..|.| ..:
Zfish    24 RRGSQGSRSKSGKKRKSGEKSTRVERNEIEKEQCAITSAAWFFTPLWVPGAKTQRLGFRMG-KES 87

  Fly    58 ERPRPLPDD--DSIRSRLISAAHLDLDVGLELASDSTAVPANGGIRPKSEGACPEMKVETISAAT 120
            |:.:...||  ||.||..               |.|:....||         |.|   ...:|.|
Zfish    88 EKVKAWLDDYSDSARSYF---------------SRSSRNWMNG---------CAE---RAQAAQT 125

  Fly   121 SPPPPRPLHGTSGPVAISLPFVGRNSSKTPEEMELEYEANVEAESRDIMTP-----------LRR 174
            ....||......|...:|.|..|                    .||.::.|           :.:
Zfish   126 LITEPRSKTTVLGRRRVSHPMSG--------------------PSRALVHPVTMQRPQSCLSVHQ 170

  Fly   175 NTRPLSVSFGGGGGRSALQPEIVEAIRSLDVPA-LRLNNLSFDGSTDPKGERNHAEEEPLTPGHD 238
            ...|.|:..|||....:|||     |.....|. .|....||.||..|.|:
Zfish   171 TDHPESLDQGGGHFPRSLQP-----IHFTFSPCRSRSRCSSFLGSPRPAGQ-------------- 216

  Fly   239 HEDSVVLRRKVNCLERKAHSLYERRLPRVPKLHLLVAGKKNPPTEEEEFRTFQRNLMDLKYPTVL 303
                  .||..:|                                     .|...|.......:.
Zfish   217 ------CRRGESC-------------------------------------AFMMQLFSGLIGALQ 238

  Fly   304 PPNPPLKALLVFHKSDSICEAITAACQRHQLDVTLV-KSKEEALDTLQKSYATAQCYHLIIIDAR 367
            ..|..|:.|      ..:||...|  ||..|.:|.| |:..:.|..:.:|.|.:     :..:|:
Zfish   239 DKNLHLQVL------QHLCEVTEA--QRVSLSMTTVDKTGGKCLGPVLQSDADS-----VHSNAQ 290

  Fly   368 SSKNLD-AEHIAR-----TIRHTHG-------------------------HHLTTIIAV------ 395
            :..|.. .|::.|     |::..|.                         :|...:|.|
Zfish   291 TKWNKSIMEYVVRTGRPLTVKDAHEDPRFQVELYEGEEDRPTSVLSVPVLNHKKEVIGVVLAVNK 355

  Fly   396 ---CKKSFFEKDDVLIALLDAGVNRCVAETTNLAMCS----------VELKQILHSIIRPHNVMS 447
               |..|||.:.|..:......:...:.|...|...|          :||.|::     .|:..|
Zfish   356 TRTCASSFFSEQDEKVLSSHMMLFGLILENHQLCESSEQENKRNQVLLELAQLV-----SHDYPS 415

  Fly   448 TQQALYTALHRLKEVVL-----------ITDDLLRIQYANRATERLLNMRLDEI---ISKQLEDI 498
            ..    ..|..|..|:|           |:||     .:.|...|::.:..:||   :.....|.
Zfish   416 VD----AMLGELAAVILPITKAQFCTVFISDD-----SSTRLFSRMVRIEYEEIECSVHCSSRDC 471

  Fly   499 FVSDLSTISEQCKNIKEFDGILTVRRKSQEGIPMHVRVVPVACIGSAPTHLIFNFDVPGGQMDFI 563
            ...|:|.|                       ..|||:       .|..|..|.|..:
Zfish   472 DTDDVSYI-----------------------YAMHVQ-------NSMETLNIVNRPI-------- 498

  Fly   564 ATLPQPKEAPRGSLHSVRRCSFDVRSIASDGLRRTSLAKLTSLPLEAPITKIINLLSQVQENCSA 628
             ::..|                 :||:....||    .|.:|           |::...|
Zfish   499 -SMVSP-----------------IRSLIGAPLR----GKQSS-----------NVIGVCQ----- 525

  Fly   629 DEARLIDKVLEFLKREGLYSPQMKEIRTDDPIATDLIGALLTGPSVYSSRRSSNDSIIRTGSSTR 693
                |::|.:...:.|..       .|.|:.:..|.        :||.:......:..:|...||
Zfish   526 ----LVNKRMAIAESEAF-------SRADEHLLEDF--------AVYCALGLQKFNTQQTADKTR 571

  Fly   694 TAAIVPAKMKSNPI-------------------IMELLDESLSWDFDIFKLEEITDYHPLLYLGM 739
            ....|..::.|..|                   .:.|||...| |||:.: ..:|.....::|.:
Zfish   572 VRLAVTKEVLSYQISASQEEINELEEAAIPSADTLSLLDFGFS-DFDMSQ-NAMTQAVVRMFLDL 634

  Fly   740 EMFRRFDVFATLNIDENVCKAWLAVIEAHYRKSNTYHNSTHAADVMQATGAFITQLTNKDMLVMD 804
            .:.:.|      .||......|:..::..||.:..|||.:||....|...|.:.....|..|  .
Zfish   635 NLPQEF------KIDYKALCQWVLSVQKCYRSNVVYHNWSHALRTAQCMFAMLQTKELKSNL--S 691

  Fly   805 RMEEATALIAAAAHDVDHPGRSSAFLCNSNDALAVLYNDLTVLENHHAAITFKLTLGDDKINIFK 869
            .:|....:||:.:||:||.|.:::::..||..||.||.. :.||:||..:.. |.|.:....|..
Zfish   692 SLEVLALMIASLSHDLDHRGVNNSYIQRSNQPLAQLYGQ-SSLEHHHYDMCL-LILNNPGSQILS 754

  Fly   870 NLDKETYKSARSTIIDMILATEMTRHFEHLAKFVSVFGGEEPRDHNP--QTDEETSILMRRMLIK 932
            :|....|.:....|...||||::...||...||..:      .::|.  ..||....|:|.||:.
Zfish   755 SLSVNEYMACLQMIEKNILATDLAIFFEKRTKFFKL------AENNSCLWKDEGHRELLRSMLMT 813

  Fly   933 VADVSNPARPMQFCIEWARRIAEEYFMQTDEEKQRHLPI-VMPMFDRATCS-IPKSQIGFIEYII 995
            .:|:....:|.......|..:|.|::.|.|.|| |.|.| .:.:.||...| :|:.|:.:|:.|.
Zfish   814 ASDICAITKPWPVQKRIAELVATEFYAQGDREK-RELNIQPIDVMDRENASRLPQMQVDYIDGIC 877

  Fly   996 QDMMHA---------------------WESFID 1007
            ..:..|                     |:..:|
Zfish   878 SPLYEALAFICESCSPLKEGCSKNRKHWQEMVD 910

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pde8	NP_001163275.2	PAS	459..539	CDD:238075	17/93 (18%)
		PAS_9	463..539	CDD:290162	15/89 (17%)
		PDEase_I	775..1016	CDD:278654	70/258 (27%)
pde5aa	XP_009305135.1	GAF	245..388	CDD:214500	28/155 (18%)
		GAF	415..558	CDD:307634	40/246 (16%)
		PDEase_I	664..894	CDD:306695	68/240 (28%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D904682at2759
OrthoFinder	1	1.000	-	-		FOG0000302
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	2.010