Protein Alignment Pi3K59F and Pik3cb

DIOPT Version :10

• Sequence 1: NP_477133.1
• Gene: Pi3K59F / 37733
• FlyBaseID: FBgn0015277
• Length: 949
• Species: Drosophila melanogaster
• Sequence 2: NP_445933.1
• Gene: Pik3cb / 85243
• RGDID: 620917
• Length: 1070
• Species: Rattus norvegicus
• Alignment Length: 939
• Identity: 229/939 - (24%)
• Similarity: 369/939 - (39%)
• Gaps: 279/939 - (29%)

  Fly    44 ILRFSGLYSEEHPSFQVRLQVFNQGRPYCLPVTSSYKAFGKRWSWNEWVTLPLQFSDLPRSAML- 107
            :::.:.|.:||.....||..:|:.....|..|.||..:......|||.:...:...||||.|.|
  Rat   337 LVKGNKLNTEETVKVHVRAGLFHGTELLCKTVVSSEISGKNDHIWNEQLEFDINICDLPRMARLC 401

  Fly   108 --VLTILDCSGAGQTTVIGGTSISMFGKDGMFRQGMYDLRVWLGVEGDGNFPSRTPGKGKESSK- 169
              |..:||                               :|                |.|:|:|
  Rat   402 FAVYAVLD-------------------------------KV----------------KTKKSTKT 419

  Fly   170 ---SQMQRLGKLAKKHRNGQVQKVDWLDRLTF------REIEVINEREKRMSDYMFLMIEFPAIV 225
               |:.|.:.|..|.|     ..|.|::.:.|      |..:||........|.:
  Rat   420 INPSKYQTIRKAGKVH-----YPVAWVNTMVFDFKGQLRSGDVILHSWSSFPDEL---------- 469

  Fly   226 VDDMYNYAVVYFEPEGDVK---YKLPAKPKLVSVPDSEIQ------MENLVERKHHRLARSERSG 281
             ::|.|       |.|.|:   |...|....:..|:::.|      .:.::| |...:|..:.:.
  Rat   470 -EEMLN-------PMGTVQTNPYAENATALHIKFPENKKQPYYYPPFDKIIE-KAAEIASGDSAN 525

  Fly   282 ISDRDAKPTASIRDQLHTIVYRYPPTYVLSSEEQDLVWKFR-FYLSSHKKALTKFLKCINW-KLE 344
            :|.|..|...::   |..|:.| .|...|...|.||:|..| ....:..::|.|.|..|.| |||
  Rat   526 VSSRGGKKFLAV---LKEILDR-DPLSQLCENEMDLIWTLRQDCRENFPQSLPKLLLSIKWNKLE 586

  Fly   345 DEVTQALWMLANWAPMDVEDALELLSPTFTHPQVRKYAVSRLAQAPDEDLLLYLLQLVQALKYED 409
            | |.|...:|..|..:...:|||||...:....||:|||..|.|..||:|..|||||||.||||
  Rat   587 D-VAQLQALLQIWPKLPPREALELLDFNYPDQYVREYAVGCLRQMSDEELSQYLLQLVQVLKYE- 649

  Fly   410 PRHIVHLHGCIFPERDVVRSILDDNGSLLDQSSLSDLSATSSGLHGSVIPANQRAASVLAAIKSD 474
                                      ..||
  Rat   650 --------------------------PFLD----------------------------------- 653

  Fly   475 KSVSPGSAGGSGSGGQGSVALPNPSAPATPGSSSLPCDSNSNALMLAEGISFGSVPANLCTFLIQ 539
                                                                    ..|..||::
  Rat   654 --------------------------------------------------------CALSRFLLE 662

  Fly   540 RACTNATLANYFYWYLSIEVEEVESVRKQDERAHDMYAMVLKMFLKVLENGNFNLRGIFYNLRKQ 604
            ||..|..:..:.:|:|..|| ...:|..|       :.::|:.:          .||...:::..
  Rat   663 RALDNRRIGQFLFWHLRSEV-HTPAVSIQ-------FGVILEAY----------CRGSVGHMKVL 709

  Fly   605 RRFIDELVK------LVKLVAKEPGNRNKKTEKFQKLLAEQDMFKVNFTNFEPIPFPLDPEIYIT 663
            .:.::.|.|      |:||.|.:. ||.|..|.....| :|..::...::.:.   ||:|.:.::
  Rat   710 SKQVEALNKLKTLNSLIKLNAMKL-NRAKGKEAMHTCL-KQSAYREALSDLQS---PLNPCVILS 769

  Fly   664 KIVPMRTSLFKSALMPAKLTFVT-SIAHHEYAAIFKHGDDLRQDQLILQMITLMDKLLRRENLDL 727
            ::...:.....|.:.|..|.:.. :........|||:|||||||.|.|||:.|||.|.:...|||
  Rat   770 ELYVEKCRYMDSKMKPLWLVYSNRAFGEDAVGVIFKNGDDLRQDMLTLQMLRLMDLLWKEAGLDL 834

  Fly   728 KLTPYKVLATSSKHGFLQYVD-SCTVAEVLAREGNIH-----------NFFRKHHPCDNGPYGIS 780
            ::.||..|||..:.|.::.|. |.|:|::.....|:.           |:.::::..|:     .
  Rat   835 RMLPYGCLATGDRSGLIEVVSTSETIADIQLNSSNVAATAAFNKDALLNWLKEYNSGDD-----L 894

  Fly   781 AEVMDTYIKSCAGYCVITYLLGVGDRHLDNLLLTTNGKLFHIDFGYILGR-----DPKPMPPPMK 840
            ...::.:..|||||||.:|:||:||||.||:::...|:|||||||:|||.     ..|....|..
  Rat   895 DRAIEEFTLSCAGYCVASYVLGIGDRHSDNIMVKKTGQLFHIDFGHILGNFKSKFGIKRERVPFI 959

  Fly   841 LSKEMVEAM-----GGISSEHHHEFRKQCYTAYLHLRRHANVMLNLFSLMVDATVPDIALEPDKA 900
            |:.:.:..:     |  ::|....||:.|..|||.||||.|:.:.||:||:.|.:|::....|
  Rat   960 LTYDFIHVIQQGKTG--NTEKFGRFRQCCEDAYLILRRHGNLFITLFALMLTAGLPELTSVKD-- 1020

  Fly   901 VKKVEENLQLGLTDEEAVQHLQSLLDVSI 929
            ::.::::|.||.::|||::..:...|.::
  Rat  1021 IQYLKDSLALGKSEEEALKQFKQKFDEAL 1049

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pi3K59F	NP_477133.1	C2_PI3K_class_III	27..186	CDD:176042	32/148 (22%)
		PI3Ka_III	284..561	CDD:238442	63/278 (23%)
		PI3Kc_III	601..945	CDD:270628	109/358 (30%)
Pik3cb	NP_445933.1	PI3K_p85B	41..118	CDD:197539
		PI3K_rbd	180..288	CDD:197540
		C2_PI3K_class_I_beta_delta	324..501	CDD:176075	47/233 (20%)
		Nuclear localization signal (NLS). /evidence=ECO:0000250	410..418		4/23 (17%)
		PI3Ka_I	532..702	CDD:238444	66/310 (21%)
		PI3Kc_IA_beta	706..1067	CDD:270717	109/358 (30%)
		G-loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	778..784		1/5 (20%)
		Catalytic loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	916..924		5/7 (71%)
		Activation loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	935..961		10/25 (40%)