Protein Alignment Pi3K59F and Pik3ca

DIOPT Version :10

• Sequence 1: NP_477133.1
• Gene: Pi3K59F / 37733
• FlyBaseID: FBgn0015277
• Length: 949
• Species: Drosophila melanogaster
• Sequence 2: NP_032865.2
• Gene: Pik3ca / 18706
• MGIID: 1206581
• Length: 1068
• Species: Mus musculus
• Alignment Length: 910
• Identity: 209/910 - (22%)
• Similarity: 356/910 - (39%)
• Gaps: 276/910 - (30%)

  Fly    60 VRLQVFNQGRPYCLPV-TSSYKAFGKRWSWNEWVTLPLQFSDLPRSAMLVLTILDCSGAGQTTV- 122
            ||..:::.|.|.|..| |........|  ||||:...:...||||:|.|.|:|  ||..|:...
Mouse   356 VRTGIYHGGEPLCDNVNTQRVPCSNPR--WNEWLNYDIYIPDLPRAARLCLSI--CSVKGRKGAK 416

  Fly   123 -----IGGTSISMFGKDGMFRQGMYDLRVW------------LGVEGDGNFPSRTPGKGKESSKS 170
                 :...:|::|........|...|.:|            :||.|.        ...||:...
Mouse   417 EEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGS--------NPNKETPCL 473

  Fly   171 QMQRLGKLAKKHRNGQVQKVDWLDRLTFREIEVINEREKRMSDYMFLMIEFPAI-VVDDMYNYAV 234
            :::                .||...                      :::||.: |:::..|::|
Mouse   474 ELE----------------FDWFSS----------------------VVKFPDMSVIEEHANWSV 500

  Fly   235 VYFEPEGDVKYKLPAKPKLVSVPDSEIQMENLVERKHHRLARSERSGISDRDAKPTASIRDQLHT 299
               ..|....|             |...:.|       ||||......:|::.......||.|..
Mouse   501 ---SREAGFSY-------------SHTGLSN-------RLARDNELRENDKEQLRALCTRDPLSE 542

  Fly   300 IVYRYPPTYVLSSEEQDLVWKFRFYLSSHKKALTKFLKCINWKLEDEVTQALWMLANWAPMDVED 364
            |          :.:|:|.:|..|.|..:..:.|.|.|..:.|...|||.|...::.:|.|:..|.
Mouse   543 I----------TEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQ 597

  Fly   365 ALELLSPTFTHPQVRKYAVSRLAQ-APDEDLLLYLLQLVQALKYEDPRHIVHLHGCIFPERDVVR 428
            |:|||...:..|.||.:||..|.: ..|:.|..||:||||.||||
Mouse   598 AMELLDCNYPDPMVRSFAVRCLEKYLTDDKLSQYLIQLVQVLKYE-------------------- 642

  Fly   429 SILDDNGSLLDQSSLSDLSATSSGLHGSVIPANQRAASVLAAIKSDKSVSPGSAGGSGSGGQGSV 493
            ..||:
Mouse   643 QYLDN------------------------------------------------------------ 647

  Fly   494 ALPNPSAPATPGSSSLPCDSNSNALMLAEGISFGSVPANLCTFLIQRACTNATLANYFYWYLSIE 558
                                                  .|..||:::|.||..:.::|:|:|..|
Mouse   648 --------------------------------------LLVRFLLKKALTNQRIGHFFFWHLKSE 674

  Fly   559 VEEVESVRKQDERAHDMYAMVLKMFLKVLENGNFNLRGIFYNLRKQRRFIDELVKLVKLVAKEPG 623
            :.. ::|.::.....:.|.....|:||              :|.:|...:::|:.|..::.:|
Mouse   675 MHN-KTVSQRFGLLLESYCRACGMYLK--------------HLNRQVEAMEKLINLTDILKQE-- 722

  Fly   624 NRNKKTEKFQ-KLLAEQDMFKVNFTN-FEPIPFPLDPEIYITKIVPMRTSLFKSALMPAKLTF-- 684
             :..:|:|.| |.|.|| |.:.:|.: .:....||:|...:..:......:..||..|..|.:
Mouse   723 -KKDETQKVQMKFLVEQ-MRQPDFMDALQGFLSPLNPAHQLGNLRLEECRIMSSAKRPLWLNWEN 785

  Fly   685 ---VTSIAHHEYAAIFKHGDDLRQDQLILQMITLMDKLLRRENLDLKLTPYKVLATSSKHGFLQY 746
               ::.:.......|||:|||||||.|.||:|.:|:.:.:.:.|||::.||..|:.....|.::.
Mouse   786 PDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEV 850

  Fly   747 V-DSCTVAEVLAREG----------NIHNFFRKHHPCDNGPYGISAEVMDTYIKSCAGYCVITYL 800
            | :|.|:.::..:.|          .:|.:.:     |.....|....:|.:.:|||||||.|::
Mouse   851 VRNSHTIMQIQCKGGLKGALQFNSHTLHQWLK-----DKNKGEIYDAAIDLFTRSCAGYCVATFI 910

  Fly   801 LGVGDRHLDNLLLTTNGKLFHIDFGYILGRDPKPM-----PPPMKLSKEMVEAMGGISSEH---- 856
            ||:||||..|:::..:|:|||||||:.|....|..     ..|..|:::.:..:...:.|:
Mouse   911 LGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQEYTKTR 975

  Fly   857 -HHEFRKQCYTAYLHLRRHANVMLNLFSLMVDATVPDIALEPDKAVKKVEENLQLGLTDEEAVQH 920
             ...|::.||.|||.:|:|||:.:||||:|:.:.:|::....|  :..:.:.|.|..|::||:::
Mouse   976 EFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDD--IAYIRKTLALDKTEQEALEY 1038

  Fly   921  920
            Mouse  1039  1038

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pi3K59F	NP_477133.1	C2_PI3K_class_III	27..186	CDD:176042	33/144 (23%)
		PI3Ka_III	284..561	CDD:238442	55/277 (20%)
		PI3Kc_III	601..945	CDD:270628	101/348 (29%)
Pik3ca	NP_032865.2	PI3K_p85B	31..108	CDD:197539
		PI3K_rbd	173..292	CDD:197540
		C2_PI3K_class_I_alpha	325..484	CDD:176043	35/177 (20%)
		PI3Ka_I	525..696	CDD:238444	57/299 (19%)
		PI3Kc_IA_alpha	695..1064	CDD:270719	104/369 (28%)
		G-loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	771..777		2/5 (40%)
		Catalytic loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	912..920		5/7 (71%)
		Activation loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	931..957		8/25 (32%)