Protein Alignment Pi3K59F and Pik3c2a

DIOPT Version :10

• Sequence 1: NP_477133.1
• Gene: Pi3K59F / 37733
• FlyBaseID: FBgn0015277
• Length: 949
• Species: Drosophila melanogaster
• Sequence 2: NP_035213.2
• Gene: Pik3c2a / 18704
• MGIID: 1203729
• Length: 1686
• Species: Mus musculus
• Alignment Length: 927
• Identity: 217/927 - (23%)
• Similarity: 346/927 - (37%)
• Gaps: 293/927 - (31%)

  Fly    66 NQGRPYCLPVTS----SYKAFGKRWSWNEWVTLPLQFSDLPRSAMLVLTIL----DCSGAGQTT- 121
            :.|:....|:.|    :||.|.....|:|.:..|:|.|.||..::|.||:.    ..||:...:
Mouse   715 HNGKDLFKPIQSKKVGTYKNFFYLIKWDELIIFPIQISQLPLESVLHLTLFGVLNQSSGSSPDSN 779

  Fly   122 -------VIGGTSISMFGKDGMFRQGMYDLRVWLGVEGDGNFPSRTPGKGKESSKSQMQRLGKLA 179
                   .:|..|:::|........|...|.:|                               .
Mouse   780 KQRKGPEALGKVSLTLFDFKRFLTCGTKLLYLW-------------------------------T 813

  Fly   180 KKHRN---GQVQKVDWLDRLTFREIEVINEREKRMSDYMFLMIEFPAIVVDDMYNYAVVYFEPEG 241
            ..|.|   |.:.|..:           :.||       :.|.::||:...|      ::|..|:.
Mouse   814 SSHTNSIPGAIPKKSY-----------VMER-------IVLQVDFPSPAFD------IIYTSPQI 854

  Fly   242 DVKYKLPAKPKLVSVPDSEIQMENLVERKHHRLARSERSGISDRDAKPTASIRDQLHTIVYRYPP 306
            |                     .|::::              |:.....:.|:.:|..|::| ..
Mouse   855 D---------------------RNIIQQ--------------DKLETLESDIKGKLLDIIHR-DS 883

  Fly   307 TYVLSSEEQDLVWKFRFYLSSHKKALTKFL-KCINWKLEDEVTQALWMLANWAPMDVEDALELLS 370
            ::.||.|::..:|:.|:|...|...|.|.| ...|||..: :.:...:|..|.|:....|||||.
Mouse   884 SFGLSKEDKVFLWENRYYCLKHPNCLPKILASAPNWKWAN-LAKTYSLLHQWPPLCPLAALELLD 947

  Fly   371 PTFTHPQVRKYAVSRLAQAPDEDLLLYLLQLVQALKYEDPRHIVHLHGCIFPERDVVRSILDDNG 435
            ..|...:||..|||.:....|::|...|.|.|||||||     ::|:
Mouse   948 AKFADQEVRSLAVSWMEAISDDELADLLPQFVQALKYE-----IYLN------------------ 989

  Fly   436 SLLDQSSLSDLSATSSGLHGSVIPANQRAASVLAAIKSDKSVSPGSAGGSGSGGQGSVALPNPSA 500
                                                                             
Mouse   990 ----------------------------------------------------------------- 989

  Fly   501 PATPGSSSLPCDSNSNALMLAEGISFGSVPANLCTFLIQRACTNATLANYFYWYLSIEVEEVESV 565
                                          ::|..||:.||..|..:|:..||.|.
Mouse   990 ------------------------------SSLVRFLLSRALGNIQIAHSLYWLLK--------- 1015

  Fly   566 RKQDERAHD-----MYAMVLKMFLKVLENGNFNLRGIFYNLRKQRRFIDELVKLVKLVAKEPGNR 625
                :..||     .|..||...|.|   |.   :|:...|.||.:    ||:|:..||::....
Mouse  1016 ----DALHDTHFGSRYEHVLGALLSV---GG---KGLREELSKQMK----LVQLLGGVAEKVRQA 1066

  Fly   626 NKKTEKFQKLLAEQDMFKV-NFTNFEPIPFPLDPEIYITKIVPMRTSLFKSALMPAKLTFVTS-I 688
            :..|   ::::.::.|.:| :|........||.|.:...::.....|.|.|..||.|:|.|.: .
Mouse  1067 SGST---RQVVLQKSMERVQSFFLRNKCRLPLKPSLVAKELNIKSCSFFSSNAMPLKVTMVNADP 1128

  Fly   689 AHHEYAAIFKHGDDLRQDQLILQMITLMDKLLRRENLDLKLTPYKVLATSSKHGFLQYVD-SCTV 752
            ...|...:||.|:|||||.|.||||.:|||:..:|.|||::..::.|:|....|.::.|. |.|:
Mouse  1129 LGEEINVMFKVGEDLRQDMLALQMIKIMDKIWLKEGLDLRMVIFRCLSTGRDRGMVELVPASDTL 1193

  Fly   753 AEVLAREGNIHNF--------FRKHHPCDNGPYGISAEVMDTYIKSCAGYCVITYLLGVGDRHLD 809
            .::....|...:|        .||::|.:. .|..::|   .:|.||||.||.||:||:.|||.|
Mouse  1194 RKIQVEYGVTGSFKDKPLAEWLRKYNPSEE-EYEKASE---NFIYSCAGCCVATYVLGICDRHND 1254

  Fly   810 NLLLTTNGKLFHIDFGYILG---------RDPKPMPPPMKLSKEMVEAMGGISSE--HHHEFRKQ 863
            |::|.:.|.:||||||..||         ||    ..|..|:.:|...:.|....  ....|...
Mouse  1255 NIMLRSTGHMFHIDFGKFLGHAQMFGSFKRD----RAPFVLTSDMAYVINGGEKPTIRFQLFVDL 1315

  Fly   864 CYTAYLHLRRHANVMLNLFSLMVDATVPDIALEPDKAVKKVEENLQLGLTDEEAVQHLQSLLDVS 928
            |..||..:|:..|:.|||.|||:.:.:|::....|  :|.|.:.||...||.||......|::.|
Mouse  1316 CCQAYNLIRKQTNLFLNLLSLMIPSGLPELTSIQD--LKYVRDALQPQTTDAEATIFFTRLIESS 1378

  Fly   929 ITAVMPALVEQIHRFTQ 945
            :.::.......||...|
Mouse  1379 LGSIATKFNFFIHNLAQ 1395

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pi3K59F	NP_477133.1	C2_PI3K_class_III	27..186	CDD:176042	26/138 (19%)
		PI3Ka_III	284..561	CDD:238442	54/277 (19%)
		PI3Kc_III	601..945	CDD:270628	115/365 (32%)
Pik3c2a	NP_035213.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..26
		Interaction with clathrin, sufficient to induce clathrin assembly. /evidence=ECO:0000250|UniProtKB:O00443	2..142
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	41..68
		PI3K_rbd	420..513	CDD:395642
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	615..640
		C2A_PI3K_class_II	677..849	CDD:175979	34/188 (18%)
		PI3Ka_II	869..1038	CDD:238441	60/304 (20%)
		PI3Kc_C2_alpha	1043..1395	CDD:270720	115/368 (31%)
		G-loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	1111..1117		2/5 (40%)
		Catalytic loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	1247..1255		4/7 (57%)
		Activation loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	1266..1292		10/29 (34%)
		PX_PI3K_C2_alpha	1426..1534	CDD:132822
		Interaction with PtdIns(4,5)P2-containing membranes. /evidence=ECO:0000269|PubMed:16338929	1488..1493
		C2B_PI3K_class_II	1560..1681	CDD:176027
		Nuclear localization signal. /evidence=ECO:0000250|UniProtKB:O00443	1606..1617