DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Menl-2 and NADP-ME3

DIOPT Version :9

Sequence 1:	NP_001027424.2	Gene:	Menl-2 / 3772692	FlyBaseID:	FBgn0029153	Length:	600	Species:	Drosophila melanogaster
Sequence 2:	NP_197960.1	Gene:	NADP-ME3 / 832657	AraportID:	AT5G25880	Length:	588	Species:	Arabidopsis thaliana

Alignment Length:	560	Identity:	212/560 - (37%)
Similarity:	334/560 - (59%)	Gaps:	12/560 - (2%)

- Green bases have known domain annotations that are detailed below.

  Fly    26 VYDDE---MEQITRP-------DWHVVMNGKYNKGLAFTIKERQRLGIMGLMPCSVRSMDDQMNA 80
            ||.::   ::|:..|       .:.::.:.:||||||||.|||....|.||:|..|.|.|.|...
plant    27 VYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYITGLLPPVVLSQDVQERK 91

  Fly    81 ALANFEARPTDIARFTYLSAVHHRHRRLYYRFIKENIEKSLPIVYTPTVGDVVATYGLNFQQAIS 145
            .:.|.......:.|:..|..:..|:.||:|:.:.:|:|:.||:|||||||:....||..:::...
plant    92 VMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQG 156

  Fly   146 LFISIHDKGHIRDLMHNWVDEGVKAICVTDGGRVLGLGDMGANAMGISLGKMILYTALGSIPPST 210
            |:||:.:||.|.:::.||...|::.|.||||.|:|||||:|...|||.:||:.||||||.|.||.
plant   157 LYISLKEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSA 221

  Fly   211 LMPVCLDVGTDNQALLQDPLYVGARIPRVKGPEYDELVDEFMESAVKCFGNNTFIHFEDFATPNA 275
            .:|:.:||||:|:.||.:..|:|.:..|..|.||.|.:.|||.:..:.:|....:.|||||..:|
plant   222 CLPITIDVGTNNEKLLNNEFYIGLKQKRANGEEYAEFLQEFMCAVKQNYGEKVLVQFEDFANHHA 286

  Fly   276 LKFLEKYQYKYCCFNDDIQGTGATGLAAFINVERITGRKLEDTVFLFVGAGSAALGIANMLAMEL 340
            .:.|.||...:..||||||||.:..||..|..:::.|:.|.|..|||:|||.|..|||.::|:::
plant   287 FELLSKYCSSHLVFNDDIQGTASVVLAGLIAAQKVLGKSLADHTFLFLGAGEAGTGIAELIALKI 351

  Fly   341 -EVRGIPAEEATKNIYLMDVNGILTPESPNPPEMGK-IFIKSMEPMKDMMAVLKKLKPSVLVGAT 403
             :..|.|.:|..|.|:|:|..|::..|.....:..| .:....:|:|:::|.:..:||:||:|.:
plant   352 SKETGKPIDETRKKIWLVDSKGLIVSERKESLQHFKQPWAHDHKPVKELLAAVNAIKPTVLIGTS 416

  Fly   404 GVGGIFNEEVLKTMAKNHERPAVFPLSNPTANSECTAEQAFTHTEGRVLFGSGSPFPPVVINGKR 468
            |||..|.:||::.||..:|:|.:..|||||:.:|||||:|:|.|:||.:|.|||||.||..:||:
plant   417 GVGKTFTKEVVEAMATLNEKPLILALSNPTSQAECTAEEAYTWTKGRAIFASGSPFDPVQYDGKK 481

  Fly   469 YRPAQANNCLTFPGIALAAITAKARYLPNEVFSVVSHELARNTPQELLDEGTLFPPIKDAHNVAF 533
            :.|.|||||..|||:.|..|.:.|..:.:::....|..||....:|....|.::||..:...::.
plant   482 FTPGQANNCYIFPGLGLGLIMSGAIRVRDDMLLAASEALASQVTEENFANGLIYPPFANIRKISA 546

  Fly   534 NVGVAMTQYLIDNDLSNVYPKPDDICEFVKRSLYKFDYRN 573
            |:..::.....:..|::..|:|.|:.:..:..:|...|||
plant   547 NIAASVGAKTYELGLASNLPRPKDLVKMAESCMYSPVYRN 586

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Menl-2	NP_001027424.2	PLN03129	8..573	CDD:215594	210/558 (38%)
		malic	102..283	CDD:278802	79/180 (44%)
		NADB_Rossmann	293..567	CDD:304358	98/275 (36%)
NADP-ME3	NP_197960.1	PLN03129	20..588	CDD:215594	212/560 (38%)
		malic	113..293	CDD:278802	78/179 (44%)
		NAD_bind_1_malic_enz	304..582	CDD:133454	99/277 (36%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0281
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG60768
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000475
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100205
Panther	1	1.100	-	-	O	PTHR23406
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X340
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	8	7.780