Protein Alignment Pex6 and SPAST

DIOPT Version :10

• Sequence 1: NP_001027403.2
• Gene: Pex6 / 3772165
• FlyBaseID: FBgn0033564
• Length: 899
• Species: Drosophila melanogaster
• Sequence 2: NP_055761.2
• Gene: SPAST / 6683
• HGNCID: 11233
• Length: 616
• Species: Homo sapiens
• Alignment Length: 316
• Identity: 100/316 - (31%)
• Similarity: 156/316 - (49%)
• Gaps: 45/316 - (14%)

  Fly   594 KNLTDMQSSFADSLGAPKVPK---VYWSDIGGLAKLKDEIQSSIGLP-LKHVHLMGKNLRRSGIL 654
            ||..::.|:.|:.:....|..   |.:.||.|....|..:|..:.|| |:.....|......|:|
Human   315 KNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQDLAKQALQEIVILPSLRPELFTGLRAPARGLL 379

  Fly   655 LYGPPGTGKTLVAKAVATECNLSFLSVQGPELLNMYVGQSEQNVREVFSRARSAAPCVLFLDELD 719
            |:||||.|||::|||||.|.|.:|.::....|.:.|||:.|:.||.:|:.||...|.::|:||:|
Human   380 LFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVD 444

  Fly   720 SLAPNRGVAGDSGGVMD---RVVSQLLAEMDGM-SDGDTSKPIFILAATNRPDLIDPALLRPGRF 780
            ||...|     ..|..|   |:.::.|.|.||: |.||..  :.::.|||||..:|.|:||  ||
Human   445 SLLCER-----REGEHDASRRLKTEFLIEFDGVQSAGDDR--VLVMGATNRPQELDEAVLR--RF 500

  Fly   781 DKLFYVGPCSTAEDKAAVLRAQTQRFALDAGVDMEQIAE--RLKSEMSGADLYSICSNAWLSAVR 843
            .|..||   |...::..:|..:.......:.:..:::|:  |:....||:||.::..:|.|..:|
Human   501 IKRVYV---SLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIR 562

  Fly   844 RTIDGHLSGTISEKELVPENV---------IVQEEDFTKSFNKFVPSISAKDLEYF 890
                          ||.||.|         .::..|||:|..|...|:|.:.||.:
Human   563 --------------ELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAY 604

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pex6	NP_001027403.2	SpoVK	<367..600	CDD:440232	2/5 (40%)
		RPT1	<609..883	CDD:440835	93/292 (32%)
SPAST	NP_055761.2	Required for interaction with RTN1. /evidence=ECO:0000269|PubMed:16602018	1..300
		Required for midbody localization. /evidence=ECO:0000269|PubMed:18997780	1..194
		Required for interaction with ATL1. /evidence=ECO:0000269|PubMed:16339213, ECO:0000269|PubMed:16815977	1..80
		Required for nuclear localization. /evidence=ECO:0000269|PubMed:15147984	1..50
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..43
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000269|PubMed:15269182	50..87
		Nuclear export signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:16026783	59..67
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000269|PubMed:18997780	112..196
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:15269182	114..200
		MIT_spastin	116..195	CDD:239142
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	224..266
		Sufficient for microtubule severing. /evidence=ECO:0000269|PubMed:15269182	228..616		100/316 (32%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000269|PubMed:15269182	270..328		4/12 (33%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	278..312
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	309..312
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:23272056	310..312
		RecA-like_spastin	343..506	CDD:410932	67/171 (39%)
		AAA_lid_3	533..587	CDD:465537	16/67 (24%)
		Vps4_C	<580..612	CDD:462762	9/25 (36%)