DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment mus201 and ERCC5

DIOPT Version :9

Sequence 1:	NP_001027230.1	Gene:	mus201 / 3772069	FlyBaseID:	FBgn0002887	Length:	1236	Species:	Drosophila melanogaster
Sequence 2:	NP_000114.3	Gene:	ERCC5 / 2073	HGNCID:	3437	Length:	1186	Species:	Homo sapiens

Alignment Length:	1272	Identity:	362/1272 - (28%)
Similarity:	587/1272 - (46%)	Gaps:	277/1272 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MGVTGLWKLIEPCGKPVPVETLEGKILAVDISIWLHQVVKGFQDNKGSALSNAHLLGLFHRLCKL 65
            |||.|||||:|..|:.|..|.||||||||||||||:|.:||.:|..|:::.|.|||.||||||||
Human     1 MGVQGLWKLLECSGRQVSPEALEGKILAVDISIWLNQALKGVRDRHGNSIENPHLLTLFHRLCKL 65

  Fly    66 LYYRVRPVFIFDGCVPQLKRDTIARRQQQRNKLSNEADRIQALLLQSLAKEKVVQQAL-GKNAEL 129
            |::|:||:|:|||..|.||:.|:.:|:|:::..|:::.:....||::..|.:.::.|. .|..|.
Human    66 LFFRIRPIFVFDGDAPLLKKQTLVKRRQRKDLASSDSRKTTEKLLKTFLKRQAIKTAFRSKRDEA 130

  Fly   130 LLKSPVKRPPPAKKNDEDDLFKLPELPAASVQDNQDESEQDTSASASDSSFDESTARHSYNSSLQ 194
            |       |...:...|:||:.||.|.......:::|.|::.....:    .:...:..:..:.|
Human   131 L-------PSLTQVRRENDLYVLPPLQEEEKHSSEEEDEKEWQERMN----QKQALQEEFFHNPQ 184

  Fly   195 AIDVKSQHFRNLPADVRHEILTDIKETRKQSSWGRLHE-LPARSDDFCSFQMKRLLKRRAVQESL 258
            |||::|:.|.:||.:|:||||||:||..|:..  .|.| :|..||||..:|:|.|||:..:.:.:
Human   185 AIDIESEDFSSLPPEVKHEILTDMKEFTKRRR--TLFEAMPEESDDFSQYQLKGLLKKNYLNQHI 247

  Fly   259 EQAEQEM----GGHTLTYAELCDFFNEEGILTPTAIEQCTRQISSDEHTRFLLVRDLKKKAMEST 319
            |..::||    .||.....|     :|.|.|.    |..:|::.|::.:.::|::.::.|.:...
Human   248 EHVQKEMNQQHSGHIRRQYE-----DEGGFLK----EVESRRVVSEDTSHYILIKGIQAKTVAEV 303

  Fly   320 KQE--------------VKMEMIEEVPAEEE-DEKPSTSTKKEAVKSVDLGTEFDEDLAKALSMS 369
            ..|              ||....|::..|:| |..|.:.....|:::..||:..:|:|
Human   304 DSESLPSSSKMHGMSFDVKSSPCEKLKTEKEPDATPPSPRTLLAMQAALLGSSSEEEL------- 361

  Fly   370 MEETKVYDEKDYEYDSDQELRLNRAQTKQLRHAAKGPARAYMIEYGGMNDEEVGNIMEATQFNDT 434
                          :|:     ||.|.:    ....||
Human   362 --------------ESE-----NRRQAR----GRNAPA--------------------------- 376

  Fly   435 QSLEKLLEITTVPTDMADNSIEEAKLISQAIEESKQLSQAIEESKKNLNEDK-VEIV---DTDTD 495
                                         |::|.....:.:...|:.|::|: |::.   |..|.
Human   377 -----------------------------AVDEGSISPRTLSAIKRALDDDEDVKVCAGDDVQTG 412

  Fly   496 SDLEEVMEV----QELDKGKKNLEICVDITGQADSNDLFADIFEDGEANKIEKTISVEEDDDFIE 556
            ....|.|.:    :..|:|.|                     ..||:......|::..      .
Human   413 GPGAEEMRINSSTENSDEGLK---------------------VRDGKGIPFTATLASS------S 450

  Fly   557 VKDSEELKLDTEDENKPITNKSIKE-------SNEVKPFIDEIIEVKDSQEAVPAE--------- 605
            |..:||....|.:..:| |:...||       ..|..|..||.: :||.::.:|.|
Human   451 VNSAEEHVASTNEGREP-TDSVPKEQMSLVHVGTEAFPISDESM-IKDRKDRLPLESAVVRHSDA 513

  Fly   606 ---PN---LKPDLESILNDLKKQTAAVKDIQ----------------LNVNEEEKPKPK------ 642
               ||   |.|...:..|.:.|.....:.::                |:.::|.|.||.
Human   514 PGLPNGRELTPASPTCTNSVSKNETHAEVLEQQNELCPYESKFDSSLLSSDDETKCKPNSASEVI 578

  Fly   643 -----VEISSILDELKVKMADVKNITLDNVKLSNSVPIILSSDDESTLKSSKIVPKQELIELCDS 702
                 .|.|||:......:.:|:|:...|.|...:  .:.:..::.|.:|:    .|:||.:
Human   579 GPVSLQETSSIVSVPSEAVDNVENVVSFNAKEHEN--FLETIQEQQTTESA----GQDLISI--- 634

  Fly   703 DDNKNNRLSPNKTPS-KNKSIKDFFETSYVVKRTPDKSQASNETSPGTPKTPKPFFRKRTPKSGR 766
                ...:.|.:..| :::|...|.|...|:        :..|.....|:|.||
Human   635 ----PKAVEPMEIDSEESESDGSFIEVQSVI--------SDEELQAEFPETSKP----------- 676

  Fly   767 KRASDANEDSDEEVSPTK--RSSKASKSLFEPKEPEEEKTVDPEEIIKDAAEALKSQK--TSEEL 827
                 .:|..:||:..|:  .:...|:||.......::...:|:|..|||.::|...:  ..|||
Human   677 -----PSEQGEEELVGTREGEAPAESESLLRDNSERDDVDGEPQEAEKDAEDSLHEWQDINLEEL 736

  Fly   828 QELATNLAQERKELEIERNRQDRMGMSISQRMSIDCQELLRLFGIPYIVAPMEAEAQCAFLNATD 892
            :.|.:||..::..|:.::.:|:|:..:::.:|.::.||||||||||||.||||||||||.|:.||
Human   737 ETLESNLLAQQNSLKAQKQQQERIAATVTGQMFLESQELLRLFGIPYIQAPMEAEAQCAILDLTD 801

  Fly   893 LTHGTITDDSDIWLFGGRTVYKNFFAQNKHVMEFRAEQIEQTFNCNRGKLIQLACLVGSDYTTGI 957
            .|.|||||||||||||.|.||:|||.:||.|..::..........:|.|||.||.|:|||||.||
Human   802 QTSGTITDDSDIWLFGARHVYRNFFNKNKFVEYYQYVDFHNQLGLDRNKLINLAYLLGSDYTEGI 866

  Fly   958 HGIGAVTALEILASFSGQDANGPGICNQSVLQTLIKFRDWW-QAHKCSNLPPGSSARLALRKKLK 1021
            ..:|.|||:|||..|.|..           |:.|:||.:|| :|.|...:.| :.....::|||:
Human   867 PTVGCVTAMEILNEFPGHG-----------LEPLLKFSEWWHEAQKNPKIRP-NPHDTKVKKKLR 919

  Fly  1022 NIELHEGFPSGAVVEAYLAPTIDDNRDAFSWGTPDVESIREFTRKSFGWTTSKTDDILMPVMKKI 1086
            .::|..|||:.||.||||.|.:||::.:|.||.||::.||||.::.|||..:|||:.|.||:|::
Human   920 TLQLTPGFPNPAVAEAYLKPVVDDSKGSFLWGKPDLDKIREFCQRYFGWNRTKTDESLFPVLKQL 984

  Fly  1087 NEKKIQGSIRNYFTAKSALRVQQPHVSKRVQLAIDKMSGKIDETPEKPKKVTRTRRAKAAPPTDD 1151
            :.::.|..|.::|    .|..|:...:||::  ..:::..:.....|.|:...:.....:...:.
Human   985 DAQQTQLRIDSFF----RLAQQEKEDAKRIK--SQRLNRAVTCMLRKEKEAAASEIEAVSVAMEK 1043

  Fly  1152 DLAIADVATKAARPKRGKRKAAPESVVLDGELPSTSQ 1188
            :..:.|.| |....|||......||..|..:..|.|:
Human  1044 EFELLDKA-KGKTQKRGITNTLEESSSLKRKRLSDSK 1079

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mus201	NP_001027230.1	rad2	1..1122	CDD:273166	349/1204 (29%)
		PIN_XPG	1..>121	CDD:189038	61/119 (51%)
		PIN_XPG	<814..1084	CDD:189038	126/272 (46%)
		H3TH_XPG	940..1045	CDD:188624	45/105 (43%)
ERCC5	NP_000114.3	rad2	1..1029	CDD:273166	351/1219 (29%)
		N-domain. /evidence=ECO:0000305\|PubMed:32522879	1..78		49/76 (64%)
		DNA-binding, may bind to the undamaged single-strand DNA of the DNA repair bubble. /evidence=ECO:0000269\|PubMed:32821917, ECO:0000312\|PDB:6TUW, ECO:0007744\|PDB:6TUX	31..67		22/35 (63%)
		Spacer region. /evidence=ECO:0000269\|PubMed:16246722	79..785		187/879 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	306..342		8/35 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	354..385		11/116 (9%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	404..473		16/96 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	510..533		5/22 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	667..724		16/72 (22%)
		I-domain. /evidence=ECO:0000305\|PubMed:32522879	786..881		57/94 (61%)
		DNA-binding, may bind to the undamaged single-strand DNA of the DNA repair bubble. /evidence=ECO:0000269\|PubMed:32821917, ECO:0000312\|PDB:6TUW, ECO:0007744\|PDB:6TUX	820..836		8/15 (53%)
		DNA-binding, H2TH (helix-2turn-helix) motif which binds double-stranded DNA. /evidence=ECO:0000269\|PubMed:32821917, ECO:0000312\|PDB:6TUW, ECO:0007744\|PDB:6TUX	848..880		21/31 (68%)
		DNA-binding, may bind double-stranded DNA. /evidence=ECO:0000269\|PubMed:32821917, ECO:0000312\|PDB:6TUW, ECO:0007744\|PDB:6TUX	912..918		1/5 (20%)
		Interaction with PCNA. /evidence=ECO:0000269\|PubMed:9305916	981..1009		6/31 (19%)
		Interaction with ERCC6/CSB. /evidence=ECO:0000269\|PubMed:16246722	1011..1186		13/72 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1056..1081		8/24 (33%)
		Nuclear localization signal 1. /evidence=ECO:0000305\|PubMed:26812207	1057..1074		6/16 (38%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1095..1186
		Nuclear localization signal 2. /evidence=ECO:0000305\|PubMed:26812207	1169..1186
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	129	1.000	Domainoid score	I5279
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	445	1.000	Inparanoid score	I1652
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1094524at2759
OrthoFinder	1	1.000	-	-		FOG0004850
OrthoInspector	1	1.000	-	-		oto89866
orthoMCL	1	0.900	-	-		OOG6_101242
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1052
SonicParanoid	1	1.000	-	-		X3423
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	9	8.990