Protein Alignment Msp300 and Evpl

DIOPT Version :10

• Sequence 1: NP_001188695.1
• Gene: Msp300 / 3771968
• FlyBaseID: FBgn0261836
• Length: 13540
• Species: Drosophila melanogaster
• Sequence 2: NP_001100536.1
• Gene: Evpl / 303687
• RGDID: 1306141
• Length: 2029
• Species: Rattus norvegicus
• Alignment Length: 2437
• Identity: 474/2437 - (19%)
• Similarity: 837/2437 - (34%)
• Gaps: 771/2437 - (31%)

  Fly 11383 AAQQQLAHLCQ----NARAFQRDFPGKKMPQDVHNAFETSKNIANNIQAERERVLQLQSLAEEYE 11443
            ||.|:||.|..    ||...:||.            .||.|    .:|.:|:...|.|:|..:.|
  Rat    34 AATQELALLISRMQANADQVERDI------------LETQK----KLQQDRQNGAQNQALQHQQE 82

  Fly 11444 --QTLKEFTKITVLADKLVESPIVSSSLEQLNNEVQKQRKFFVNLSHCRAMLESLEENIDSETRE 11506
              :.|||       |:.|         |:.|..:|.|.|:    |.|.:|  |.:|::|
  Rat    83 TGRNLKE-------AEVL---------LKDLFLDVDKARR----LKHPQA--EEIEKDI------ 119

  Fly 11507 KHSELHKELYNRATSLLDKASERSSKLVQAASRWTVLEKGMRDELQWLQVAQQRVPDLSAVTSAD 11571
                  |:|:.|.|   .:.:|     .:|.....||...:...:.|.:|.:|:...:..
  Rat   120 ------KQLHERVT---QECAE-----YRALYEKIVLPPDVGPRVDWTRVLEQKQKLICE----- 165

  Fly 11572 YDQYTTLYQSLSNDISHHYVKMTQLSGIANKLQLLVQAP--NLVEETNEALIVLLKLREEV--AL 11632
             ..|......|...::.|.:...::.....:|:.|| .|  |.:......|:.....|.:.  :|
  Rat   166 -GHYGPGMAELEQQVAEHNILQREIEAYGQQLRTLV-GPDANAIRNQYRDLLKAASWRRQSLGSL 228

  Fly 11633 YLHRLLVFKEIWVQYEQQTDKLEAFVREAEQELRNIQIPSQPTHQPIEHMRQFWEIKARFELHNN 11697
            |.|.....|::....:||.       |..:|:..::........:..||.:|       .||
  Rat   229 YTHLQGCTKQLGALADQQG-------RILQQDWSDLMPDPAGVRREYEHFKQ-------HEL--- 276

  Fly 11698 VRTDTGLSFEKSLQVIPLADEMLQRQFHAQLEDRWQAVAQ----AIELIQHNIVECLSSEDVPAD 11758
                  |:.|:|:.               ||||..:.:.:    ||..||.:            .
  Rat   277 ------LAQEQSIN---------------QLEDDAERMVELGHPAIGPIQAH------------Q 308

  Fly 11759 EKLKMVERELQEIYLTMTSMKGVIKNEEELCL----YIERVQVLRTRVGFIGNELGRIGLQEPAI 11819
            |.|||   |.|              |...||:    .::.|:..|.             .||.|.
  Rat   309 EALKM---EWQ--------------NFLNLCICQESQLQHVEDYRR-------------FQEEAD 343

  Fly 11820 EPEKVGELFSLSHKISTQIAEELEGASVLRDQLQAIQEGISNQRKHQAKISVILDECEAAERQGA 11884
            ...:.  |..||..:.|:.:.....:||...:|                    |.:.||.|:|.|
  Rat   344 SASQT--LAKLSSNLDTKYSFGAGDSSVSPTEL--------------------LLQLEAEEKQLA 386

  Fly 11885 DVLEKAVADCQAAGEEL------------------VISWQ--EIMRIRQMLHTLPMRLKMSVSP- 11928
             :.|:.:.:.|...:|:                  :..|.  |:..:|...:|    ||.:..|
  Rat   387 -IAERTIGELQRRSQEVAPLPQRRNPPKQPLHVDSICDWDSGEVQLLRGERYT----LKDNADPY 446

  Fly 11929 -----------------------------VKLERDISQLQDDHAFLESKCTNIMAILRSRLAVWL 11964
                                         .|..|..|:||.    |:.|.:...:.|::..|..|
  Rat   447 TWVVQGPGGETKSAPAACLCIPAPDPEAVAKASRLASELQT----LKQKLSTEKSRLKNASAEHL 507

  Fly 11965 RYERQLELVHGSVQETDFMMELIRVHGQVDYERLRKATERLEGLAGDLHNREQLIDELKGAAKPL 12029
            :..:|...  ||........:|:....|:|               |||...|:.:  |..|..||
  Rat   508 QQGQQAPA--GSAPADPQAQKLLSQMTQLD---------------GDLGQVERQV--LSWARSPL 553

  Fly 12030 IESCDVQIVEQIESAVQEAVVAWNDTSENLQQLRTRYQRAVELWDKYRNASAAVKNSIDQQMDAV 12094
            .:|..   ::.:|..:|..    ..|:::||.|                  .|.|.:..|:.:|.
  Rat   554 NDSSP---LKDLEGRIQSC----EGTAQHLQNL------------------GAEKEAAQQECEAF 593

  Fly 12095 KSLEQPLDALQHAKVCQDNLTTQNDRILELRDIVA-KIAADVGL-----DASALMQGELDALGQR 12153
            .|.:....|.....|..:|:..:...:..|..:.. |..|.:||     :|..::||....|.|.
  Rat   594 LSTKPTGSAALQLPVVLNNVKNKYSDVHSLCYLYGEKAKAALGLEKQIQEADGIIQGFEATLAQE 658

  Fly 12154 --LAECKDAITTLANVAETQDKERKE----------LDKEVTLAK----AYFNNVQ---QDISRE 12199
              :.|...|:.  ..|:|.| ::|||          |.:::..|:    |..||.|   ||:.|:
  Rat   659 GPIPEGSGALQ--ERVSELQ-RQRKELLQQQACVLGLHRQLKAAEHACGALQNNFQEFCQDLPRQ 720

  Fly 12200 APQNPKESEEQLAAL--RAH------------LQTLARTEEQLRQLK-------ERHQNSEVAPS 12243
                    :.|:.||  |.|            ||..:.|.:||:..:       |:..:..|.||
  Rat   721 --------QRQVRALTDRYHSVGDQLDLREKILQDASLTHQQLKNSRDNLSSWLEQLPHHRVQPS 777

  Fly 12244 VASSDDDGILEV---LALWQKIFQDTFQEYHRLSTRLARSQNSSEALRLWRQYLQHVQSFLSCAI 12305
                  ||..::   |...:::.|:.          |.|.|:.:...||.|...:.:|.:
  Rat   778 ------DGPSQIAYKLQAQKRLIQEI----------LGREQDQATVARLTRDLQEALQEY----- 821

  Fly 12306 PEDYSSLREQQQLCAIHQNLLISQQSVLSETPLESELSEQYKALTNLHNETLSRIMQRNGELE-- 12368
                 .||.....|::...|.:|....|....|:..:..|.|.||..:.|..:...|:..:||
  Rat   822 -----ELRADTYRCSLEPTLAVSAPKRLRVISLKENIQAQEKNLTKAYTEVAAAEQQQLHQLEFA 881

  Fly 12369 -------------------RRVSGWNAYRQQLAALLDWLRQREAERNALQLRYIHLKRVPHLKHR 12414
                               |:.||..|:.:..:.:|.  .|.|.||          |||..::..
  Rat   882 KKMLEKKELNEDFQAIHSARQGSGSPAHTKTESEVLK--TQLEEER----------KRVAEVQRE 934

  Fly 12415 LDAMIQQLDQGEQQSKALQEQQQELARHCDD------ALATAMRMEQASIGQRISNLRAALKTWQ 12473
            |:...|||.|...|....:.:::|:.....|      .|..:.|:|:.  |:|.:.|:|.|:.
  Rat   935 LEERRQQLLQLRTQQPVARLEEKEVVEFYRDPQLKSNVLQASSRLEEE--GKRRARLQAELEV-- 995

  Fly 12474 GFLQRVTQLSESYE--------QRVNQLQQEFGAAQKLLDANSESLPTQPAAIEQLLGSLRAQRV 12530
             ..|:|..|....:        :.|.|::::.|            |.:|...:...:..||.:..
  Rat   996 -VAQKVVHLEGKRKAMQPHLLTKEVTQIERDPG------------LDSQVTQLHNEMQGLRGENA 1047

  Fly 12531 QLGAQVSAL--ESLTVTQEELKECISPHDMKTIRQRNWLLWQQHADLDYQLANLINSIEERLSLL 12593
            .|.|::..|  |.||:.|:|:                                   :::|::.:.
  Rat  1048 VLAARLEGLKDELLTLEQKEI-----------------------------------NVKEKVVVK 1077

  Fly 12594 SNYQIRYD-RISQWLQRLEQRVEKDA-----DVTAMTNPEQAAKQLEQQVNS--------ELQLR 12644
            ...::..| .:.:..|.|..::|:||     ...|:...:...|.|||.::|        |::..
  Rat  1078 EVVKVEKDLEMVKAAQTLRLQIEEDAARRKGAKEAVAKTQARIKDLEQAISSVEPKVIVKEVKKV 1142

  Fly 12645 DKEREWLLSTSRELLTLYSEPEVRSQVQQQSDSLIDRWQRLKYLAKQK-----ATKIGELKMTLL 12704
            :::...|...||....|..|......:.::...|.|:::.::   |||     ..::.|:.....
  Rat  1143 EQDPGLLKEASRLRSLLEEEKNKNVMLARELQELQDKYRAVE---KQKPKVQLQERVSEIFQVHP 1204

  Fly 12705 RLEERIALIRAWLFE-------VESQLDKPL-------------NFESYTPNVIEAKLKEHEQIQ 12749
            ..|:.|..:||.|.|       ||.:::|.|             .::..|..|:  :.:::.::.
  Rat  1205 ETEQEILRLRAQLQETGSKKSCVEREVEKLLPELEVLRAQKPVVEYKEVTQEVV--RHEKNPEVL 1267

  Fly 12750 RSIEHHSSNVGEVLN--------LVEMLLNDADSW---RTQVNTS-------------GLAASAQ 12790
            |.|:...:.:.|::|        |:. |..:.|.|   |::|.|.             .|...|:
  Rat  1268 REIDRLKAQLNELVNTHGRCQEQLIR-LQGERDEWKRERSKVETKMVNKEVVRHEKDPVLEKEAE 1331

  Fly 12791 NLEQRWKNVCSQSAERKARILTIWNLLQQLIKLTAEHKNWLGKQESQIAGFE-----RDQKSHSK 12850
            .|.|..:....   :|:|....::.|..:.:.|...      :.|.||...|     :|.|.|.:
  Rat  1332 RLRQEVREAVQ---KRRAAEDAVYELQNKYLLLERR------RPEEQIVVQEVVVTQKDPKLHEE 1387

  Fly 12851 H-----KLEE-----RQMELR-----AKLEELES-------QSVNL---RQLEQIYAKLAMSAGV 12890
            |     .|:|     ||:||.     |.:||.|:       :|..|   |:|.|:..|:......
  Rat  1388 HSRLSRSLDEEVGRRRQLELEVRQLSASVEEEEAMLSFEEDRSKKLAAERELRQLTLKIQELEKR 1452

  Fly 12891 EPENIQKLTLPTKVMVSMWRQLTPRCHALLDAIDKD----AKLMREFNNAQLEATNSLNAIQKAL 12951
            .|...:|:.:...|.:.....|.....||...:|::    .:|.||....|:|    ::.:||
  Rat  1453 PPALQEKIIMEEVVKLEKDPDLERSTEALRRELDQEKNRVIELHRECKGLQVE----VDLLQK-- 1511

  Fly 12952 EQLPSAENQQTSKAEPKAVLQRLESLEKKLQDAQQHVQQADNLAQEAKTRTKQQPQLKQLLELVS 13016
                       :|::.|.:.:.:..:||......:..:..:.|.:|...|..::..::.|.|.:.
  Rat  1512 -----------TKSQEKTIYKEVIRVEKDPVLESERARVWETLNRERAARQSREEDVRSLKERID 1565

  Fly 13017 AYTTLWQTVQTRIVTLKTTWLTRAAQAAASLPVSEAANAAVQVNTLSQRKLRQAQQMQRETSITA 13081
                       |...|:.:|..:.|:..||   .:......::..|.|:||::|||:|.|..:.:
  Rat  1566 -----------RAEALRRSWSLQKARDQAS---QDCGRLQRELRELEQQKLQKAQQLQEEGRLLS 1616

  Fly 13082 ------------KDAYIMELQTAITECQNNLDELQRTVVD--------------KTRKPG-PQKI 13119
                        :...:.:|:.||...::.:.|.:||:.|              :||:.. ..||
  Rat  1617 QKTESERQKAAQRSQALSQLEAAILHEKDKIYEKERTLRDLHTKVSREELNQETQTRETNLSTKI 1681

  Fly 13120 AKL---LGNAQSSTE-----LVKHLSHLLLTECKAD-DQAAEVDTVAELTLRFDTLQSQWKARQQ 13175
            ..|   .||..|..|     ::....:|.|.|.:.| ::........|.::..|.     |:.:|
  Rat  1682 CILEPETGNDMSPYEAYKRGIIDRGQYLQLQELECDWEEVTTSSPCGEESVLLDR-----KSGKQ 1741

  Fly 13176 HDQNASEVGRLTCPLCTQRNWQQI-DNDLWRLEQWLQFAESTQKAQS------------------ 13221
            :...|:    |.|...::..:.:. |..|...|..|..|..|:.:.|
  Rat  1742 YSIEAA----LRCRRISKEEYHRYKDGRLPISEFALLVAGETKPSSSLSIGSIISKSPVCSPGPQ 1802

  Fly 13222 -----APPSNIELLEDVTQDHREFLLDLESHKSIISSLNVVGDHLATHTLDTEKARQLRSRLEAD 13281
                 :|..::.|.||                    |..:.|.:                    |
  Rat  1803 STGFFSPGLSLGLTED--------------------SFPIAGVY--------------------D 1827

  Fly 13282 NERWNNVCINATKWQGLLQTALMGNS--EFHQTIGELVEWLQRTEQNIKASEPVDLTEERSVLET 13344
            ....|...|.|...:.:|. .:.|..  |.....|.:|:.|.|...::..:....|.|..|.
  Rat  1828 TTTDNKCSIKAAVAKNMLD-PITGQKLLEAQAATGGIVDLLSRERYSVHKAVEQGLIENTST--- 1888

  Fly 13345 KFKKFKDLRAELERCEPRVVSLQDAADQLLRSVEGSEQQSQHTYERTLSRLTDLRLRLQSLRRLS 13409
                     ..|...:.....::|...:...||..:.|:.....|..|..|.        ::.|:
  Rat  1889 ---------QRLLNAQKAFTGIEDPVTRKRLSVGEAIQKGWMPQESVLPHLL--------VQHLT 1936

  Fly 13410 GIYI---------VKLGAVLGYEGDNLGVPLHMLSS---ELLDNTTLSTSSMQAAAPNTENANNT 13462
            |..|         |....:.|...::||..|...||   :|.|..|....|.:.|......
  Rat  1937 GGLIDPKRTGRIPVPQAVLCGMISEDLGQLLQDESSYEKDLTDPITKERLSYKEAMGRCHK---- 1997

  Fly 13463 DGGDAVDGDVINTTVLARGARFLGRVARASLP 13494
               |.:.|.::...:| .|.|.. |||..:||
  Rat  1998 ---DPLSGLLLLPAML-EGYRCY-RVASPTLP 2024

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Msp300	NP_001188695.1	SPEC	17..220	CDD:238103
		EnvC	194..>393	CDD:443969
		PTZ00121	<1704..1881	CDD:173412
		SPEC	9546..9732	CDD:413338
		rne	<10668..10827	CDD:236766
		SMC_prok_B	11268..12195	CDD:274008	180/904 (20%)
		YhaN	11930..12426	CDD:443752	123/565 (22%)
		SMC_prok_B	<12205..12954	CDD:274008	180/906 (20%)
		YhaN	12817..>13241	CDD:443752	101/517 (20%)
		SPEC	13196..13374	CDD:238103	29/203 (14%)
		KASH	13484..13540	CDD:463142	5/11 (45%)
Evpl	NP_001100536.1	SPEC	228..>358	CDD:413338	41/211 (19%)
		SH3_10	404..468	CDD:407754	7/67 (10%)
		Smc	668..>1312	CDD:440809	149/750 (20%)
		Smc	<1096..1680	CDD:440809	123/629 (20%)
		Plectin	<1683..1711	CDD:459901	6/27 (22%)
		PLEC	1813..1850	CDD:197605	10/77 (13%)
		Plectin	1853..1891	CDD:459901	8/49 (16%)
		PLEC	1889..1926	CDD:197605	6/36 (17%)
		Plectin	1932..1967	CDD:459901	7/34 (21%)