DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo1 and Robo4

DIOPT Version :9

Sequence 1:	NP_476899.1	Gene:	robo1 / 37603	FlyBaseID:	FBgn0005631	Length:	1395	Species:	Drosophila melanogaster
Sequence 2:	XP_006510707.1	Gene:	Robo4 / 74144	MGIID:	1921394	Length:	1074	Species:	Mus musculus

Alignment Length:	1404	Identity:	276/1404 - (19%)
Similarity:	412/1404 - (29%)	Gaps:	639/1404 - (45%)

- Green bases have known domain annotations that are detailed below.

  Fly    30 WLLPAWLLLVLVASNGLPAVRGQYQSPRIIEHPTDLVVKKNEPATLNCKVEGKPEPTIEWFKDGE 94
            |.||. |||.::....|.:      .|:|:.||.|.:::.:.||.:.|:..|:|.|||.|..:|:
Mouse    23 WPLPL-LLLFIMGGEALDS------PPQILVHPQDQLLQGSGPAKMRCRSSGQPPPTIRWLLNGQ 80

  Fly    95 PVSTNEKKSHRVQFKDGALFFYR-TMQGKKEQDG-------GEYWCVAKNRVGQAVSRHASLQIA 151
            |:|......|.: ..||.|..:| ::||:.:.|.       |.|.|.|.||:|.||||.|.|.:|
Mouse    81 PLSMATPDLHYL-LPDGTLLLHRPSVQGRPQDDQNILSAILGVYTCEASNRLGTAVSRGARLSVA 144

  Fly   152 VLRDDFRVEPKDTRVAKGETALLECGPPKGIPEPTLIWIKDGVPLDDLKAMSFGASSRVRIVDGG 216
            ||::||:::|:||....||:.:||||||.|.|:|::.|.|||.||    .:..|.    |.|.|.
Mouse   145 VLQEDFQIQPRDTVAVVGESLVLECGPPWGYPKPSVSWWKDGKPL----VLQPGR----RTVSGD 201

  Fly   217 NLLISNVEPIDEGNYKCIAQNLVGTRESSYAKLIVQVKPYFMKEPKDQVMLYGQTATFHCSVGGD 281
            :|::|..|..|.|.|.|:|.|..|.|||..|::.:|       |.:|.
Mouse   202 SLMVSRAEKNDSGTYMCMATNNAGQRESRAARVSIQ-------ESQDH----------------- 242

  Fly   282 PPPKVLWKKEEGNIPVSRARILHDEKSLEISNITPTDEGTYVCEAHNNVGQISARASLIVHAPPN 346

Mouse   243 ----------------------------------------------------------------- 242

  Fly   347 FTKRPSNKKVGLNGVVQLPCMASGNPPPSVFWTKEGVSTLMFPNSSHGRQHVAADGTLQITDVRQ 411
                                             ||.:..|
Mouse   243 ---------------------------------KEHLELL------------------------- 249

  Fly   412 EDEGYYVCSAFSVVDSSTVRVFLQVSSLDERPPPIIQIGPANQTLPKGSVATLPCRATGNPSPRI 476
                             .||:.|:                 |.||.             ||.|
Mouse   250 -----------------AVRIQLE-----------------NVTLL-------------NPEP-- 265

  Fly   477 KWFHDGHAVQAGNRYSIIQGSSLRVDDLQLSDSGTYTCTASGERGETSWAATLTVEKPGSTSLHR 541
                             ::|                                   .|||
Mouse   266 -----------------VKG-----------------------------------PKPG------ 272

  Fly   542 AADPSTYPAPPGTPKVLNVSRTSISLRWAKSQEKPGAVGPIIGYTVEYFSPDLQTGWIVAAQRVG 606
               ||.:                  |.|..|       ||
Mouse   273 ---PSVW------------------LSWKVS-------GP------------------------- 284

  Fly   607 DTQVTISGLTPGTSYVFLVRAENTQGISVPSGLSNTIKTIEADFDAASANDLSAARTLLTGKSVE 671
                    ..|..||..|.|.:.                        |..|..:..|
Mouse   285 --------AAPAESYTALFRTQR------------------------SPRDQGSPWT-------- 309

  Fly   672 LIDASAINASAVRLEWMLHVSADEKYVEGLRIHYKDASVPSAQYHSITVMDASAESFVVGNLKKY 736
                                   |..:.||                        :|..:|.|...
Mouse   310 -----------------------EVLLRGL------------------------QSAKLGGLHWG 327

  Fly   737 TKYEFFLTPFFETIEGQPSNSKTALTYEDVPSAPPDNIQIGMYNQTAGWVRWTPPPSQHHNGNLY 801
            ..|||.:.|......|..||.......|.||||||..:.:...|.:. :|.|.|||::.|||.:.
Mouse   328 QDYEFKVRPSSGRARGPDSNVLLLRLPEQVPSAPPQGVTLRSGNGSV-FVSWAPPPAESHNGVIR 391

  Fly   802 GYKIEVSAGNTMKVLANMTLNATTTSVLLNNLTTGAVYSVRLNSFTKAGDGPYSKPISLFMDPTH 866
            ||:: .|.||.....||.|:....|.:.:.....|: |.|::.:.|.||.|..|.|:.|.:....
Mouse   392 GYQV-WSLGNASLPAANWTVVGEQTQLEIATRLPGS-YCVQVAAVTGAGAGELSTPVCLLLGEGK 454

  Fly   867 HVH----PPRAHPSGTHDGRHEGQDLTYHNNGNI------------------------------P 897
            .:.    ||...||       ..|.|:|..:.::                              .
Mouse   455 ALSISSTPPPCRPS-------VSQFLSYIFSSSLSCLLVPLTVPLALPLSSTSQTEQAMEQSARD 512

  Fly   898 PGDINPTTHKKTTDYLSGPWLMVLVCIVLLVLVISAAISMVYFKRKHQMTKELGHLSVVSDNEIT 962
            |....|.|.::....|..|.::....::|.:|::...: .:|.:||..:....|.....|::.|.
Mouse   513 PRKHVPWTLEQLRATLRRPEVIASSAVLLWLLLLGITV-CIYRRRKAGVHLGPGLYRYTSEDAIL 576

  Fly   963 ALNINSKESLWIDHHRGWRTADTDKDSGLSESKLLSHVNSSQSNYNNSD---GGTDYAEVDTR-- 1022
            ...::..:|.|:..  .||:....:|  ||.|..|    ||:...:..|   |.......|.|
Mouse   577 KHRMDHSDSPWLAD--TWRSTSGSRD--LSSSSSL----SSRLGLDPRDPLEGRRSLISWDPRSP 633

  Fly  1023 ------NLTTFYN--CRKSPDNP-------TPYA---TTMIIGT-----SSSETC---------- 1054
                  :.:|||.  ..:.|.:|       ||.|   .:.:.||     ||...|
Mouse   634 GVPLLPDTSTFYGSLIAEQPSSPPVRPSPKTPAARRFPSKLAGTSSPWASSDSLCSRRGLCSPRM 698

  Fly  1055 --TKTTSISADKDSGTHSPYSDAFAGQVPAVPVVKSNYLQYPVEPINW------SEFL---PPPP 1108
              |.|.:..|.|....|         |..:.|:::.:   :|:|...|      |:.|   |.|.
Mouse   699 SLTPTEAWKAKKKQELH---------QANSSPLLRGS---HPMEIWAWELGSRASKNLSQSPGPN 751

  Fly  1109 EHPP---PSSTYGYAQGSPESSRKSSKSAG----------SGISTNQ------------------ 1142
            ...|   |.:...:....|:..|.||:.|.          .|.|::.
Mouse   752 SGSPGEAPRAVVSWRAVGPQLHRNSSELASRPLPPTPLSLRGASSHDPQSQCVEKLQAPSSDPLP 816

  Fly  1143 ----SILNASIHSSSSGGFSAWGVSPQYAVACP---PENVYSNPLSA------------------ 1182
                |:||:|..||....|          ::||   ..|:.|:.||:
Mouse   817 AAPLSVLNSSRPSSPQASF----------LSCPSPSSSNLSSSSLSSLEEEEDQDSVLTPEEVAL 871

  Fly  1183 ---VAGGTQNRYQITPTNQHPPQLPAYFATTGPGGAVPP---NHLPFATQRHAASEYQAGLNAAR 1241
               ::.|.:     ||||...|.         |....||   .::...|....|...:||....
Mouse   872 CLELSDGEE-----TPTNSVSPM---------PRAPSPPTTYGYISIPTCSGLADMGRAGGGVG- 921

  Fly  1242 CAQSRACNSCDALATPSPMQPPPPVPVP--------EGW 1272
               |...|.         :.||.|.|.|        .||
Mouse   922 ---SEVGNL---------LYPPRPCPTPTPSEGSLANGW 948

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo1	NP_476899.1	Ig	56..151	CDD:299845	38/102 (37%)
		I-set	56..150	CDD:254352	38/101 (38%)
		I-set	157..251	CDD:254352	39/93 (42%)
		Ig2_Robo	159..251	CDD:143201	38/91 (42%)
		I-set	255..341	CDD:254352	2/85 (2%)
		Ig3_Robo	272..341	CDD:143202	0/68 (0%)
		IG_like	351..436	CDD:214653	6/84 (7%)
		Ig	362..444	CDD:299845	6/81 (7%)
		I-set	445..531	CDD:254352	7/85 (8%)
		IGc2	459..521	CDD:197706	4/61 (7%)
		FN3	549..637	CDD:238020	10/87 (11%)
		FN3	673..762	CDD:238020	13/88 (15%)
		fn3	770..855	CDD:278470	27/84 (32%)
Robo4	XP_006510707.1	Ig	41..144	CDD:386229	38/103 (37%)
		Ig	152..236	CDD:386229	38/91 (42%)
		FN3	361..446	CDD:238020	28/87 (32%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	55	1.000	Domainoid score	I11052
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.910