DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo1 and ROBO2

DIOPT Version :9

Sequence 1:	NP_476899.1	Gene:	robo1 / 37603	FlyBaseID:	FBgn0005631	Length:	1395	Species:	Drosophila melanogaster
Sequence 2:	XP_011532283.1	Gene:	ROBO2 / 6092	HGNCID:	10250	Length:	1597	Species:	Homo sapiens

Alignment Length:	1472	Identity:	448/1472 - (30%)
Similarity:	660/1472 - (44%)	Gaps:	312/1472 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly    19 NNPSRSRSSRM-WLLPAWLLLVLVASNGLPAVRGQYQSPRIIEHPTDLVVKKNEPATLNCKVEGK 82
            :.|:.|:.:|. |....|        .|...:|.:...|||:|||:|::|.|.||.|||||.||:
Human    24 SGPTLSKLTRTPWKGKPW--------KGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGR 80

  Fly    83 PEPTIEWFKDGEPVST--NEKKSHRVQFKDGALFFYRTMQGKKEQ-DGGEYWCVAKNRVGQAVSR 144
            |.|||||:||||.|.|  ::.:|||:....|:|||.|.:.|::.: |.|.|.|||:|.:|:||||
Human    81 PTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSR 145

  Fly   145 HASLQIAVLRDDFRVEPKDTRVAKGETALLECGPPKGIPEPTLIWIKDGVPLDDLKAMSFGASSR 209
            :|||::|:||||||..|.|..||.||.|:|||.||:|.||||:.|.||.|.:||       ...|
Human   146 NASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDD-------KEER 203

  Fly   210 VRIVDGGNLLISNVEPIDEGNYKCIAQNLVGTRESSYAKLIVQVKPYFMKEPKDQVMLYGQTATF 274
            :.| .||.|:|||....|.|.|.|:..|:||.|:|..|:|.|..:|.|::.|.:||:|..:...|
Human   204 ISI-RGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEF 267

  Fly   275 HCSVGGDPPPKVLWKKEEGNIPVSRARILHDEKSLEISNITPTDEGTYVCEAHNNVGQISARASL 339
            .|.|.|||.|.|.|||::.::|..|..| .|:.:|.|.....||||||:|.|.|.||::.|.|:|
Human   268 RCQVQGDPQPTVRWKKDDADLPRGRYDI-KDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATL 331

  Fly   340 IVH----APPNFTKRPSNKKVGLNGVVQLPCMASGNPPPSVFWTKEGVSTLMFPN---SSHGRQH 397
            .|.    |||.|..||.::.|.....|..||...|||.|:|||.|||...|:|||   ..:.|..
Human   332 TVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCS 396

  Fly   398 VAADGTLQITDVRQEDEGYYVCSAFSVVDSSTVRVFLQVSS-LDERPPPIIQIGPANQTLPKGSV 461
            |:..|.|.||::::.|.|||:|.|.:|..|...:..|:|:. |.:||||||..|||||||.....
Human   397 VSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGT 461

  Fly   462 ATLPCRATGNPSPRIKWFHDGHAVQAGN-RYSIIQGSSLRVDDLQLSDSGTYTCTASGERGETSW 525
            |.|.|:|||:|.|.|.|..:|......: |.:|.:..:|::.:|::||:|||||.|:...|||||
Human   462 ALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSW 526

  Fly   526 AATLTVEKPGSTSLHRAADPSTYPAPPGTPKVLNVSRTSISLRWAKSQEKPGAVG--PIIGYTVE 588
            :|.|.|.:.|:| :.:..|.|..|.||..|:|.:|::.|::|.|     :||..|  |...|.:|
Human   527 SAVLDVTESGAT-ISKNYDLSDLPGPPSKPQVTDVTKNSVTLSW-----QPGTPGTLPASAYIIE 585

  Fly   589 YFSPDLQTGWIVAAQRVGDTQVTISGLTPGTSYVFLVRAENTQGISVPSGLSNTIKTIEADFDAA 653
            .||..:...|...|..|..|..|:.||.|.|.|:|:|||.|.||:|.||.:|:.::|.:.. ..|
Human   586 AFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDIS-PPA 649

  Fly   654 SANDLSAARTLLTGKSVELIDASAINASAVRLEWMLHVSADEKYVEGLRIHYKDAS--VPSAQYH 716
            ...|....:..|....|.|.:...:..:.|::.|.  |....::::|.|:.|:..|  ..::.:.
Human   650 QGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWT--VDRQPQFIQGYRVMYRQTSGLQATSSWQ 712

  Fly   717 SITVMDASAESFVVGNLKKYTKYEFFLTPFFETIEGQPSNSKTALTYEDVPSAPPDNI---QIGM 778
            ::.....:..|.|:.||||...||..:.|:|...:|..|.|||..|.|:.|||||.::   .:|.
Human   713 NLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGS 777

  Fly   779 YNQTAGWVRWTPPPSQHHNGNLYGYKIEVSAGNTMKVLANMTLNATTTSVLLNNLTTGAVYSVRL 843
            ||.|:..|.|.|||..|.||.:..||| ...||..:...|.|::|...||::..|..|..|.|.:
Human   778 YNSTSISVSWDPPPPDHQNGIIQEYKI-WCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEV 841

  Fly   844 NSFTKAGDGPYSKPISLFMDPTHHVHPPRAHPSGTHDGRHEGQDLTYHNNGNIPPGDINPTTHKK 908
            .:.|.||.|..|:|..:.:                  ||.....:|.:||          :..::
Human   842 AASTSAGVGVKSEPQPIII------------------GRRNEVVITENNN----------SITEQ 878

  Fly   909 TTDYLSGPWLMVLVCIVLLVLVISAAISMVYFKRK-------HQMTKELGHLSVVSDNEITA-LN 965
            .||.:..|..:..:.....|:::..:| .:|::||       :.:|.:.|...::|:..... ||
Human   879 ITDVVKQPAFIAGIGGACWVILMGFSI-WLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLN 942

  Fly   966 INSKESLWIDHHRGWRTADTDKDSGLSESKLLSHVNSSQSNYNN--------------------- 1009
            .......|:            .||..:.|..:::.||..:...|
Human   943 AGDPSYPWL------------ADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATML 995

  Fly  1010 SDGGTDYAEVDTRNLTTFYNCRKSPDNPTPYATTMIIGTSSSETCTKTTSISADKDSGTHSPYSD 1074
            |||.. |:.:|....|: ||........||||||.|:                      ||....
Human   996 SDGAI-YSSIDFTTKTS-YNSSSQITQATPYATTQIL----------------------HSNSIH 1036

  Fly  1075 AFAGQVPAVPVVKSNYLQYPVEPINWSEFLPPP----------PEH---------PPPSSTYGYA 1120
            ..|..:|. |..||: :|...:.:.:...||..          |::         .|.:.:..::
Human  1037 ELAVDLPD-PQWKSS-IQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFS 1099

  Fly  1121 QGSPESS--RKSSKSAGSGISTNQSILNASIHSSSSGGFSAWGVSP---------------QYAV 1168
            ..|..:|  |..|.|.|.| ...:...|:|....::|  |.|...|               .|||
Human  1100 TTSSHNSSERSGSLSGGKG-GKKKKNKNSSKPQKNNG--STWANVPLPPPPVQPLPGTELEHYAV 1161

  Fly  1169 A-----------CPP---------------------------ENVYSNPLSAVAGGTQNRYQITP 1195
            .           |||                           ..|.|:|  |::.|.|:...:||
Human  1162 EQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSP--AISFGQQSTATLTP 1224

  Fly  1196 ---------------------------------TNQHPPQLPAYFATTGPGGAVPPNHLPFATQR 1227
                                             :|..|||.|           |||  |.:.:..
Human  1225 SPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPP-----------VPP--LGYVSGA 1276

  Fly  1228 HAASEYQAGLNAARCAQSRACNSCDALATPSPMQPPPPVP--------------VPEGWYQPVHP 1278
             ..|:.:..:     |...|.:..:||..|.|::.....|              :..||
Human  1277 -LISDLETDV-----ADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGW------ 1329

  Fly  1279 NSHPMHPTSSNHQIYQCSSECSDHSRSSQSHKRQLQLEEHGS 1320
                              ...||..|:..||:..:.....||
Human  1330 ------------------GSASDEDRNFSSHRSSVGSSSDGS 1353

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo1	NP_476899.1	Ig	56..151	CDD:299845	54/97 (56%)
		I-set	56..150	CDD:254352	54/96 (56%)
		I-set	157..251	CDD:254352	44/93 (47%)
		Ig2_Robo	159..251	CDD:143201	42/91 (46%)
		I-set	255..341	CDD:254352	37/85 (44%)
		Ig3_Robo	272..341	CDD:143202	31/68 (46%)
		IG_like	351..436	CDD:214653	34/87 (39%)
		Ig	362..444	CDD:299845	35/85 (41%)
		I-set	445..531	CDD:254352	40/86 (47%)
		IGc2	459..521	CDD:197706	23/62 (37%)
		FN3	549..637	CDD:238020	35/89 (39%)
		FN3	673..762	CDD:238020	22/90 (24%)
		fn3	770..855	CDD:278470	32/87 (37%)
ROBO2	XP_011532283.1	Ig1_Robo	53..152	CDD:143317	54/98 (55%)
		Ig	160..244	CDD:325142	42/91 (46%)
		Ig	265..333	CDD:325142	31/68 (46%)
		Ig4_Robo	355..444	CDD:143203	35/88 (40%)
		IG	451..532	CDD:214652	36/80 (45%)
		FN3	549..642	CDD:238020	38/97 (39%)
		FN3	673..758	CDD:238020	22/86 (26%)
		FN3	766..859	CDD:238020	34/93 (37%)
		PTN13_u3	<1374..>1431	CDD:318742
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6690
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H43188
Inparanoid	1	1.050	609	1.000	Inparanoid score	I923
Isobase	1	0.950	-	0.917438	Normalized mean entropy	S5483
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	1	1.000	-	-		mtm8568
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3358
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	11	10.840