DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo1 and Igdcc4

DIOPT Version :9

Sequence 1:	NP_476899.1	Gene:	robo1 / 37603	FlyBaseID:	FBgn0005631	Length:	1395	Species:	Drosophila melanogaster
Sequence 2:	XP_006511369.1	Gene:	Igdcc4 / 56741	MGIID:	1858497	Length:	1312	Species:	Mus musculus

Alignment Length:	1413	Identity:	294/1413 - (20%)
Similarity:	486/1413 - (34%)	Gaps:	417/1413 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly    27 SRMWLLPAWLLLVLVASNGL--------------------PAVRG--QYQSP---------RIIE 60
            ||.|..||..:|   |..||                    |..||  .::.|         ...|
Mouse    29 SREWASPAPAML---ARRGLWGLAPRGCVSLLCTAWHRACPPARGPCAWELPLPQETTVKLSCDE 90

  Fly    61 HPTDLVVKKNEPATLNCKV---EGKPEPTIEWFKDGEPVSTNEKKSHRVQFKDGALFFYRTMQGK 122
            .|..:::...:...|:|.:   ...|...:.|.|||:.|..:| ..|.:  .:|:|:....::.:
Mouse    91 GPLQVILGPEQAVVLDCTLGATAAGPPTRVTWSKDGDTVLEHE-NLHLL--PNGSLWLSSPLEQE 152

  Fly   123 KEQD----------GGEYWCVAKNRVGQAVSRHASLQIAVLRDDFRVEPKDTRVAKGETALLECG 177
            ...|          .|.|.|:|.:.:|...|:.|.:::|.| :||.:.|:...|.:..||..||
Mouse   153 DSDDEEALRIWKVTEGSYSCLAHSPLGVVASQVAVVKLATL-EDFSLHPESQIVEENGTARFEC- 215

  Fly   178 PPKGIPEPTLIWIKDGVPLDDLKAMSFGASSRVRIVDGGNLLISNVEPIDEGNYKCIAQNLVGTR 242
            ..||:|.|.:.|.||.|.:.:        .||:..:..|.|.|.:|:..|.|:|:|:|.|....|
Mouse   216 HTKGLPAPIITWEKDQVTVPE--------ESRLITLPNGVLQILDVQDSDAGSYRCVATNSARQR 272

  Fly   243 ESSYAKLIVQVK----------PYFMKEPKDQVMLYGQTATFHCSVGGDPPPKVLWKKEEGNIPV 297
            .|..|.|.|.::          ...:..|::..::.||:....|....||.|.|.|.:::|. |:
Mouse   273 FSQEASLTVALRGSLEATRGQDVVIVAAPENTTVVSGQSVVMECVASADPTPFVSWVRQDGK-PI 336

  Fly   298 SRARILHDEKSLEISNITPTDEGTYVCEAHNNVGQ--ISARASLIVHAPPNFTKRPSNKKVGLNG 360
            |...|:....:|.|::..|...|.|||.|:....:  .:|.|.|.|.|.|..::.|.........
Mouse   337 STDVIVLGRTNLLIASAQPRHSGVYVCRANKPRTRDFATAAAELRVLAAPAISQAPEALSRTRAS 401

  Fly   361 VVQLPCMASGNPPPSVFWTKEGVSTLMFPNSSHGRQHV-AADGTLQITDVRQEDEGYYVCSAFSV 424
            ..:..|.|||.|.|::.|..:|:     |...:||..| ...|:|.||.:..:|.|||.|.|.:.
Mouse   402 TARFVCRASGEPRPALHWLHDGI-----PLRPNGRVKVQGGGGSLVITQIGLQDAGYYQCVAENS 461

  Fly   425 VDSSTVRVFLQVSSLDERPPPIIQIGPANQTLPKGSVATLPCRATGNP----SPRIKW----FHD 481
            ..::.....|.|         :::.|     ||     :.|.|.|..|    |..:.|    .|.
Mouse   462 AGTACAAAPLAV---------VVREG-----LP-----SAPTRVTATPLSSSSVLVAWERPELHS 507

  Fly   482 GHAVQAGNRYSIIQG--------------SSLRVDDLQLSDSGTYTCTASGERGETSWAATLTVE 532
            ...:.....|...:|              :.|:|.||:.:....:...|..:.|         ..
Mouse   508 EQIIGFSLHYQKARGVDNVEYQFAVNNDTTELQVRDLEPNTDYEFYVVAYSQLG---------AS 563

  Fly   533 KPGSTSLHRAADPSTYPAPPGTPKVLNVSRTSISLRWAKSQEKPGAV--GPIIGYTVEY------ 589
            :..|.:|....|.....||..|....|.|  .|.:.|.   ..|.::  |.::.|.:||
Mouse   564 RTSSPALVHTLDDVPSAAPQLTLSSPNPS--DIRVAWL---PLPSSLSNGQVLKYKIEYGLGKEE 623

  Fly   590 ---FSPDLQTGWIVAAQRVGDTQVTISGLTPGTSYVFLVRAENTQGISVPS--------GLSN-- 641
               ||.::..         .:||:|::.|.|...|...:.|....|..|||        |:.|
Mouse   624 DQVFSTEVPG---------NETQLTLNSLQPNKVYRVRISAGTGAGYGVPSQWMQHRTPGVHNQS 679

  Fly   642 -------------TIKTI---------------------------EADFD------AASANDLSA 660
                         .::::                           |||.|      ...|.|:..
Mouse   680 HVPFAPAELKVRAKMESLVVSWQPPPHPTQISGYKLYWREVGTEEEADGDRPPGGRGDQAWDVGP 744

  Fly   661 AR-----------TLLTGKSVEL----------------------------------------ID 674
            .|           .|:.|:..|:                                        :.
Mouse   745 VRLKKKVKQYELTQLVPGRLYEVKLVAFNKHEDGYAAVWKGKTEKAPTPDLPIQRGPPLPPAHVH 809

  Fly   675 ASAINASAVRLEWMLHVSADEKYVE--------GLR----IHYKDASVPSAQYHSITVMDASAES 727
            |.:.:::::.|.|........|.|.        |||    :.|..:.:||.        .:|.|.
Mouse   810 AESNSSTSIWLRWKKPDFTTVKIVNYTVRFGPWGLRNASLVTYYTSPIPSP--------GSSGED 866

  Fly   728 FVVGNLKKYTKYEFFLTPFFETIEGQPSNSKTALTYEDVPSAPPDNIQIGMYNQTAGWVRWTPPP 792
            .::|.||.:|||||.:......::|...:.....|..|.||.||.::::.....:...:.|.||.
Mouse   867 ILIGGLKPFTKYEFAVQSHGVDMDGPFGSVVERSTLPDRPSTPPSDLRLSPLTPSTVRLHWCPPT 931

  Fly   793 SQHHNGNLYGYKIEVSAGNTM--KVLANMTLNATTTSVLLNNLTTGAVYSVRLNSFTKAGDGPYS 855
            ..  ||.:..|.|..|..:|.  .....:|......|..::.|.:...|..::.:.|:.|.||:|
Mouse   932 EP--NGEIVEYLILYSNNHTQPEHQWTLLTTEGNIFSAEVHGLESDTRYFFKMGARTEVGPGPFS 994

  Fly   856 KPISLFMDPTHHVHPPRAHPSGTHDGRHEGQDLTYHNNGNIPPGDINPTTHKKTTDYL-----SG 915
            :.                            ||:.              |..|..:|.|     :|
Mouse   995 RL----------------------------QDVI--------------TLQKTFSDSLDVHAVTG 1017

  Fly   916 PWLMVLVCIVLLVLVISAAISMVYFKRKHQM----------------TKELGHLSVVSDNEITAL 964
              ::|.||:.||.|:......:.....:..:                ...||..||.:.:|:
Mouse  1018 --IIVGVCLGLLCLLACMCAGLRRSSHREALPGLSSSGTPGNPALYTRARLGPPSVPAAHEL--- 1077

  Fly   965 NINSKESLWIDHHRGWRTADTDKDS--------GLSESKLLSH----VNSSQSNYNNSDGGTDYA 1017
                 |||.....:.|....:|.:.        |.|.|....|    ::.:|:      ||.::|
Mouse  1078 -----ESLVHPRPQDWSPPPSDVEDKAEVHSLMGGSVSDCRGHSKRKISWAQA------GGPNWA 1131

  Fly  1018 EVDTRNLTTFYNCR-KSPDNPTPYATTMII---GTSSSETCTKTTSISADKDSGTHSPYSDAFAG 1078
                   .::..|. .....|.|..|..::   ||..: ...:.....|.|.:|...| |.|...
Mouse  1132 -------GSWAGCELPQGSGPRPALTRALLPPAGTGQT-LLLQALVYDAIKSNGRKKP-SPACRN 1187

  Fly  1079 QVPAVPVVKSNY----------LQ--YPVEPINWSEFLPPPPEHPPPSSTYGYAQGSPESSRKSS 1131
            ||.|..:|.|::          ||  .|.||:. :|.||         ||.|....|..:.....
Mouse  1188 QVEAEVIVHSDFGASKGCPDLHLQDLEPEEPLT-AETLP---------STSGAVDLSQGADWLGR 1242

  Fly  1132 KSAGSGISTNQSILNASIHSSSSGGFSAWGVSPQYAV------ACPPE 1173
            :..|...:|:.......:..::|...|...:.|..|:      :|.|:
Mouse  1243 ELGGCQPTTSGPERLTCLPEAASASCSCSDLQPSTAIEEAPGKSCQPK 1290

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo1	NP_476899.1	Ig	56..151	CDD:299845	23/116 (20%)
		I-set	56..150	CDD:254352	23/115 (20%)
		I-set	157..251	CDD:254352	31/93 (33%)
		Ig2_Robo	159..251	CDD:143201	30/91 (33%)
		I-set	255..341	CDD:254352	24/87 (28%)
		Ig3_Robo	272..341	CDD:143202	21/70 (30%)
		IG_like	351..436	CDD:214653	24/85 (28%)
		Ig	362..444	CDD:299845	24/82 (29%)
		I-set	445..531	CDD:254352	18/107 (17%)
		IGc2	459..521	CDD:197706	14/83 (17%)
		FN3	549..637	CDD:238020	23/98 (23%)
		FN3	673..762	CDD:238020	22/100 (22%)
		fn3	770..855	CDD:278470	19/86 (22%)
Igdcc4	XP_006511369.1	Ig	89..190	CDD:386229	22/103 (21%)
		I-set	196..281	CDD:369462	31/93 (33%)
		IG	301..382	CDD:214652	24/81 (30%)
		Ig	401..473	CDD:386229	23/76 (30%)
		fn3	481..564	CDD:365830	15/91 (16%)
		FN3	578..672	CDD:238020	25/107 (23%)
		fn3	684..778	CDD:365830	10/93 (11%)
		FN3	802..901	CDD:238020	22/106 (21%)
		FN3	909..1001	CDD:238020	22/135 (16%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	2	1.910