DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo1 and robo2

DIOPT Version :9

Sequence 1:	NP_476899.1	Gene:	robo1 / 37603	FlyBaseID:	FBgn0005631	Length:	1395	Species:	Drosophila melanogaster
Sequence 2:	NP_001259868.1	Gene:	robo2 / 44522	FlyBaseID:	FBgn0002543	Length:	1519	Species:	Drosophila melanogaster

Alignment Length:	1549	Identity:	466/1549 - (30%)
Similarity:	707/1549 - (45%)	Gaps:	339/1549 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly    27 SRMWLLPAWLLLVLVASNGLP---AVRGQYQSPRIIEHPTDLVVKKNEPATLNCKVEGKPEPTIE 88
            ||..:.|  |||:|...|||.   |::|  ::|||||||.|..|.||:|.|.||:.||.|.|||:
  Fly    63 SRRAVFP--LLLLLAGLNGLTQVGALKG--ENPRIIEHPMDTTVPKNDPFTFNCQAEGNPTPTIQ 123

  Fly    89 WFKDGEPVSTNEKKSHRVQFKDGALFFYRTMQGKKEQDGGEYWCVAKNRVGQAVSRHASLQIAVL 153
            |||||..:.| :..|||:....|.|||.:.:..::|.|.|.|||.|||..|.|.||:|:||:|.|
  Fly   124 WFKDGRELKT-DTGSHRIMLPAGGLFFLKVIHSRRESDAGTYWCEAKNEFGVARSRNATLQVAFL 187

  Fly   154 RDDFRVEPKDTRVAKGETALLECGPPKGIPEPTLIWIKDGVPLDDLKAMSFGASSRVRIVDGGNL 218
            ||:||:||.:||||:||.||:|||.|:|.|||.:.|.|:|      :.::...:.|:||||||||
  Fly   188 RDEFRLEPANTRVAQGEVALMECGAPRGSPEPQISWRKNG------QTLNLVGNKRIRIVDGGNL 246

  Fly   219 LISNVEPIDEGNYKCIAQNLVGTRESSYAKLIVQVKPYFMKEPKDQVMLYGQTATFHCSVGGDPP 283
            .|......|:|.|:|:.:|:||||||:.|.|.|.|:|:.::.|::|..:.|.:..|.|.:||||.
  Fly   247 AIQEARQSDDGRYQCVVKNVVGTRESATAFLKVHVRPFLIRGPQNQTAVVGSSVVFQCRIGGDPL 311

  Fly   284 PKVLWKK--EEGNIPVSRARILHDEKSLEISNITPTDEGTYVCEAHNNVGQISARASLIVHAPPN 346
            |.|||::  ..||:|:.|..:|.| :||::.::|..|.|.|.|||.|.||.|:|...|.|||||.
  Fly   312 PDVLWRRTASGGNMPLRRVHVLED-RSLKLDDVTLEDMGEYTCEADNAVGGITATGILTVHAPPK 375

  Fly   347 FTKRPSNKKVGLNGVVQLPCMASGNPPPSVFWTKEGVSTLMFPNSSHGRQHVA----ADGTLQIT 407
            |..||.|:.|.:...|...|.|:|:|.|:::|:.||.|:|:.|....||..|.    ....|.|.
  Fly   376 FVIRPKNQLVEIGDEVLFECQANGHPRPTLYWSVEGNSSLLLPGYRDGRMEVTLTPEGRSVLSIA 440

  Fly   408 DVRQEDEGYYV-CSAFSVVDSSTVRVFLQVSSLDERPPPIIQIGPANQTLPKGSVATLPCRATGN 471
            ...:||.|..| |:|.:.|.|.:.|..:.|.:..|.|||||:.||.|||||..|:..||||..|.
  Fly   441 RFAREDSGKVVTCNALNAVGSVSSRTVVSVDTQFELPPPIIEQGPVNQTLPVKSIVVLPCRTLGT 505

  Fly   472 PSPRIKWFHDGHA--VQAGNRYSIIQGSSLRVDDLQL-SDSGTYTCTASGERGETSWAATLTVEK 533
            |.|::.|:.||..  ||...|.::....:|.:.|||. .|.|.|||.||...|::||:..|.::.
  Fly   506 PVPQVSWYLDGIPIDVQEHERRNLSDAGALTISDLQRHEDEGLYTCVASNRNGKSSWSGYLRLDT 570

  Fly   534 PGSTSL--HRAADPSTYPAPPGTPKVLNVSRTSISLRWAKSQEKPGAVGPIIGYTVEYFSPDLQT 596
            |.:.::  .||.:.||||.|||.|:::.....|::|.|.:|.:..|:  .::||.:|.|..:...
  Fly   571 PTNPNIKFFRAPELSTYPGPPGKPQMVEKGENSVTLSWTRSNKVGGS--SLVGYVIEMFGKNETD 633

  Fly   597 GWIVAAQRVGDTQVTISGLTPGTSYVFLVRAENTQGISVPSGLSNTIKTIEADFDAASANDLSAA 661
            ||:....||.:|..|.:||.||.:|.||:||||:.|:|:||.:|..|......|:  |..|||.|
  Fly   634 GWVAVGTRVQNTTFTQTGLLPGVNYFFLIRAENSHGLSLPSPMSEPITVGTRYFN--SGLDLSEA 696

  Fly   662 R-TLLTGKSVELIDASAINASAVRLEWMLHVSADEKYVEGLRIHYKDASVP-------------- 711
            | :||:|..|||.:||.:::::::|.|.:   .:.|||||..::.:....|
  Fly   697 RASLLSGDVVELSNASVVDSTSMKLTWQI---INGKYVEGFYVYARQLPNPIVNNPAPVTSNTNP 758

  Fly   712 ----------------------------------------------SAQYHSITVMD-ASAESFV 729
                                                          :.:|..:|::: ..|.|..
  Fly   759 LLGSTSTSASASASASALISTKPNIAAAGKRDGETNQSGGGAPTPLNTKYRMLTILNGGGASSCT 823

  Fly   730 VGNLKKYTKYEFFLTPFFETIEGQPSNSKTALTYEDVPSAPPDNIQIGMYNQTAGWVRWTPPPSQ 794
            :..|.:||.||||:.||::::||:||||:.|.|.|||||..|..::..:.|.:|.:::|..|..:
  Fly   824 ITGLVQYTLYEFFIVPFYKSVEGKPSNSRIARTLEDVPSEAPYGMEALLLNSSAVFLKWKAPELK 888

  Fly   795 HHNGNLYGYKIEV----SAGNTMKVLANMTLNATTTSVLLNNLTTGAVYSVRLNSFTKAGDGPYS 855
            ..:|.|..|.:.|    :|.|..::|.|:|::|.:.:::|.|||.|.:|:|.:.:...||.|||.
  Fly   889 DRHGVLLNYHVIVRGIDTAHNFSRILTNVTIDAASPTLVLANLTEGVMYTVGVAAGNNAGVGPYC 953

  Fly   856 KPISLFMDP-THHVHPPRAHPSGTHDGRHEGQDLTYHNNGNIPPGDIN---PTTHKKTTDYLSGP 916
            .|.:|.:|| |..:.|                             .||   |.......|.|:.|
  Fly   954 VPATLRLDPITKRLDP-----------------------------FINQRYPINQDHVNDVLTQP 989

  Fly   917 WLMVLVCIVLLVLVISAAISMVYFKRKHQMTKELGHLSVVSDN------EITALNINSKESLWID 975
            |.::|:..:|.||::|.. :||:.||||.|.|: ..|:.:..|      ::.:|:..:....|:|
  Fly   990 WFIILLGAILAVLMLSFG-AMVFVKRKHMMMKQ-SALNTMRGNHTSDVLKMPSLSARNGNGYWLD 1052

  Fly   976 HHRG---WR------TADTDKD-----------------------------SGLSESKLLSHVNS 1002
            ...|   ||      :.:..||                             ||.|....:...:.
  Fly  1053 SSTGGMVWRPSPGGDSLEMQKDHIADYAPVCGAPGSPAGGGTSSGGSGGAGSGASGGDDIHGGHG 1117

  Fly  1003 SQSN-------YNNSDGGTDYAEVDTRNLTTFYNCRKSPDNPTPYATTMIIGTSSSETCTKTTSI 1060
            |:.|       |:|..  ||||||.:.........|....:|.||||:.|:
  Fly  1118 SERNQQRYVGEYSNIP--TDYAEVSSFGKAPSEYGRHGNASPAPYATSSIL-------------- 1166

  Fly  1061 SADKDSGTHSPYSDAFAGQ-------VPAVPVVKSNYLQYPVEP--------------------- 1097
                     ||:......|       ||...      ||.|:.|
  Fly  1167 ---------SPHQQQQQQQPRYQQRPVPGYG------LQRPMHPHYQQQQHQQQQAQQTHQQHQA 1216

  Fly  1098 INWSEFLPP--------------PPEHPPPSSTYGYAQGSPESSRKSSKSAGSGISTNQSILNAS 1148
            :...:.|||              |....|..|.|......|:..::...      .|...:.|..
  Fly  1217 LQQHQQLPPSNIYQQMSTTSEIYPTNTGPSRSVYSEQYYYPKDKQRHIH------ITENKLSNCH 1275

  Fly  1149 IHSSSSGGFSAWGVSPQYAVA----CPPENVYSNPLSAVAGGTQNRYQITPTNQHPPQLPAY--- 1206
            .:.::.|...:..:|.|:|..    .||        :...|....|:::..|:|...|....
  Fly  1276 TYEAAPGAKQSSPISSQFASVRRQQLPP--------NCSIGRESARFKVLNTDQGKNQQNLLDLD 1332

  Fly  1207 --------FATTGPGGAVPPNHLPFATQ-RHAASEYQAGLNAARCAQSRACNSCDALATPSPMQP 1262
                    .|.:|.||:..|..:..:.: .||......|          ..:..:.|......|.
  Fly  1333 GSSMCYNGLADSGCGGSPSPMAMLMSHEDEHALYHTADG----------DLDDMERLYVKVDEQQ 1387

  Fly  1263 PPP-----VPVPEGWYQPVHPNSHPMHPTSSNHQIY-------------QCSSECSD--HSRSSQ 1307
            ||.     :|:.            |.||...:.|.:             :|..|.|:  ::..|.
  Fly  1388 PPQQQQQLIPLV------------PQHPAEGHLQSWRNQSTRSSRKNGQECIKEPSELIYAPGSV 1440

  Fly  1308 SHKRQLQLEEHGSSAKQRGGHHRRRAPVVQPCM--ESENENMLAEYEQRQYTSD 1359
            :.:|.|.......::.|..||:      | |.:  .|..::......|.|..||
  Fly  1441 ASERSLLSNSGSGTSSQPAGHN------VXPLLPGSSLGQSQSKSQSQSQSQSD 1488

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo1	NP_476899.1	Ig	56..151	CDD:299845	50/94 (53%)
		I-set	56..150	CDD:254352	49/93 (53%)
		I-set	157..251	CDD:254352	46/93 (49%)
		Ig2_Robo	159..251	CDD:143201	44/91 (48%)
		I-set	255..341	CDD:254352	35/87 (40%)
		Ig3_Robo	272..341	CDD:143202	31/70 (44%)
		IG_like	351..436	CDD:214653	29/89 (33%)
		Ig	362..444	CDD:299845	28/86 (33%)
		I-set	445..531	CDD:254352	39/88 (44%)
		IGc2	459..521	CDD:197706	25/64 (39%)
		FN3	549..637	CDD:238020	33/87 (38%)
		FN3	673..762	CDD:238020	30/149 (20%)
		fn3	770..855	CDD:278470	26/88 (30%)
robo2	NP_001259868.1	Ig1_Robo	90..185	CDD:143317	50/95 (53%)
		I-set	91..184	CDD:254352	49/93 (53%)
		I-set	190..279	CDD:254352	46/94 (49%)
		Ig2_Robo	193..279	CDD:143201	44/91 (48%)
		I-set	283..370	CDD:254352	35/87 (40%)
		Ig	300..370	CDD:299845	31/70 (44%)
		Ig	388..>457	CDD:299845	23/68 (34%)
		I-set	479..561	CDD:254352	36/81 (44%)
		Ig	496..561	CDD:143165	25/64 (39%)
		FN3	588..681	CDD:238020	36/94 (38%)
		FN3	<817..856	CDD:238020	18/38 (47%)
		fn3	864..952	CDD:278470	25/87 (29%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6635
eggNOG	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D173520at2759
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	1	1.100	-	-	P	PTHR12231
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X493
	7	6.920