DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo1 and robo3

DIOPT Version :9

Sequence 1:	NP_476899.1	Gene:	robo1 / 37603	FlyBaseID:	FBgn0005631	Length:	1395	Species:	Drosophila melanogaster
Sequence 2:	NP_608592.2	Gene:	robo3 / 33314	FlyBaseID:	FBgn0041097	Length:	1342	Species:	Drosophila melanogaster

Alignment Length:	1397	Identity:	455/1397 - (32%)
Similarity:	666/1397 - (47%)	Gaps:	255/1397 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly    56 PRIIEHPTDLVVKKNEPATLNCKVEGKPEPTIEWFKDGEPVSTNEKKSHRVQFKDGALFFYRTMQ 120
            |||:|||.|..|.::||||||||.||.|.|||:|:|||.|:.. ...|||:....|.|||.:.:.
  Fly    23 PRIVEHPIDTTVPRHEPATLNCKAEGSPTPTIQWYKDGVPLKI-LPGSHRITLPAGGLFFLKVVN 86

  Fly   121 GKKEQDGGEYWCVAKNRVGQAVSRHASLQIAVLRDDFRVEPKDTRVAKGETALLECGPPKGIPEP 185
            .::|.|.|.|||.|||.:|.|.||:|:||:|||||:||:||::||:|:|:||||||..|:|||||
  Fly    87 SRRETDAGIYWCEAKNELGVARSRNATLQVAVLRDEFRLEPQNTRIAQGDTALLECAAPRGIPEP 151

  Fly   186 TLIWIKDGVPLDDLKAMSFGASSRVRIVDGGNLLISNVEPIDEGNYKCIAQNLVGTRESSYAKLI 250
            |:.|.|.|..||      ...|.||||||||||.|.:....|||.|:|||:|.||.||||.|.|.
  Fly   152 TVTWKKGGQKLD------LEGSKRVRIVDGGNLAIQDARQTDEGQYQCIAKNPVGVRESSLATLK 210

  Fly   251 VQVKPYFMKEPKDQVMLYGQTATFHCSVGGDPPPKVLWKK--EEGNIPVSRARILHDEKSLEISN 313
            |.||||.::.|.||.:|.|.:.||.|.|||||.|.|||.:  ..||:|:.|..:|.| :||.:..
  Fly   211 VHVKPYIIRGPHDQTVLEGASVTFPCRVGGDPMPDVLWLRTASGGNMPLDRVSVLED-RSLRLER 274

  Fly   314 ITPTDEGTYVCEAHNNVGQISARASLIVHAPPNFTKRPSNKKVGLNGVVQLPCMASGNPPPSVFW 378
            :|..|||.|.|||.|.||.|:|..:|.|:|||.|.:||::|.|.|.......|.|.|||.|::||
  Fly   275 VTIADEGEYSCEADNVVGAITAMGTLTVYAPPKFIQRPASKSVELGADTSFECRAIGNPKPTIFW 339

  Fly   379 TKEGVSTLMFPNSS-----HGRQHVAADGTLQITDVRQEDEGYYV-CSAFSVVDSSTVRVFLQVS 437
            |.:..|||:||.:.     |..........|.:|..::.|:...: |:|.:.|.|.|.||.|.:.
  Fly   340 TIKNNSTLIFPGAPPLDRFHSLNTEEGHSILTLTRFQRTDKDLVILCNAMNEVASITSRVQLSLD 404

  Fly   438 SLDERPPPIIQIGPANQTLPKGSVATLPCRATGNPSPRIKWFHDGHAVQAGNRYSIIQGSSLRVD 502
            |.::||||||..||.|||||..|:|||.|:|.|.|||.|.|:.||..||..::.:|.....|.:.
  Fly   405 SQEDRPPPIIISGPVNQTLPIKSLATLQCKAIGLPSPTISWYRDGIPVQPSSKLNITTSGDLIIS 469

  Fly   503 DL-QLSDSGTYTCTASGERGETSWAATLTVEKPGSTSL--HRAADPSTYPAPPGTPKVLNVSRTS 564
            || :..|.|.|||.||...|:::|:..|.:|.|.:.::  :||.:.:..|:.||.||:||.:.::
  Fly   470 DLDRQQDQGLYTCVASSRAGKSTWSGFLRIELPTNPNIKFYRAPEQTKCPSAPGQPKILNATASA 534

  Fly   565 ISLRWAKSQEKPGAVGPIIGYTVEYFSPDLQTGWIVAAQRVGDTQVTISGLTPGTSYVFLVRAEN 629
            :::.|..| :|.|| ...:||:||.:..:....||..|.|:.:...|:..||.|.:|:|:|||||
  Fly   535 LTIVWPTS-DKAGA-SSFLGYSVEMYCTNQSRTWIPIASRLSEPIFTVESLTQGAAYMFIVRAEN 597

  Fly   630 TQGISVPSGLSNTI---KTIEADFDAASAND----LSAARTLLTGKS-VELIDASAINASAVRLE 686
            :.|.|.||.:|..|   |.:.....:.|...    ||...|||.... |||::|:|.:::..||.
  Fly   598 SLGFSPPSPISEPITAGKLVGVRDGSESTGTSQLLLSDVETLLQANDVVELLEANASDSTTARLS 662

  Fly   687 WMLHVSADEKYVEGLRIHYKDASVPSAQYHSITVMD--ASAESFVVGNLKKYTKYEFFLTPFFET 749
            |.:.   ..:|:||..::.::  :.|::|..:|:::  ....|..|..|.|.:.|||||.||:::
  Fly   663 WDID---SGQYIEGFYLYARE--LHSSEYKMVTLLNKGQGLSSCTVPGLAKASTYEFFLVPFYKS 722

  Fly   750 IEGQPSNSKTALTYEDVPSAPPDNIQIGMYNQTAGWVRWTPP-PSQHHNGNLYGYKIEV---SAG 810
            |.|:||||:...|.||||.|||..::...:|:|:.:::|.|| |::..||.|..|.:.|   ...
  Fly   723 IVGKPSNSRRMRTLEDVPEAPPYGMEAIQFNRTSVFLKWLPPQPNRTRNGILTSYNVVVKGLDVH 787

  Fly   811 NTMKVLANMTLNATTTSVLLNNLTTGAVYSVRLNSFTKAGDGPYSKPISLFMDPTHHVHPPRAHP 875
            ||.::..|||::|...::||.|||||..|.:.:.:.|:.|.||:|||..|.:
  Fly   788 NTTRIFKNMTIDAAAPTLLLANLTTGVTYYIAVAAATRVGVGPFSKPAVLRI------------- 839

  Fly   876 SGTHDGRHEGQDLTYHNNGNIPPGDINPTTHKKTTDYLSGPWLMVLV--CIVLLVLVISAAISMV 938
                |.|.:..|..|..         .|.:.....|:|:..|.:||:  .|.::|.::.|   :|
  Fly   840 ----DARTQSLDTGYTR---------YPISRDIADDFLTQTWFIVLLGSIIAIIVFLLGA---LV 888

  Fly   939 YFKRKHQMTKE--LGHL----SVVSDNEITALNIN------------------SKESLWIDHHRG 979
            .||| :|..|:  ||.|    ::.:..:...|.:|                  |...||||...|
  Fly   889 LFKR-YQFIKQTSLGSLHGNHAIGTVRKFPTLPLNGGGAVGAGGSNSTAAAGQSGHGLWIDPTSG 952

  Fly   980 -WRTADTDKDSGLSESKLLSHVNSSQSNYNNSDGGTDYAEVDTRNLTTFYNCRKSPDNPTPYATT 1043
             ||.|                          :.||                      ||.
  Fly   953 VWRQA--------------------------AGGG----------------------NPG----- 964

  Fly  1044 MIIGTSSSETCTKTTSISADKDSGTHSPYSDAFA--GQVPAV--------PVVKSNYLQYPVEPI 1098
               |..|   ||....:..         |:.|.|  ||.|.:        |:...:|.:     :
  Fly   965 ---GVGS---CTTKEQLPG---------YAQATAQQGQQPTLLPDYERLSPLNMPDYAE-----V 1009

  Fly  1099 NWSEFLPPPPEHPPPSSTYGYAQGSPESSRKSSKSAGSGISTNQSILNASIHSSSSGGFSAWGVS 1163
            ..|.|..|....||...      |....|.....|.|:..:|| .:.|..::
  Fly  1010 ACSTFKSPTHGAPPMGG------GGGGQSASLYDSCGAYATTN-VVANVKLY------------- 1054

  Fly  1164 PQYAVACPPENVYSNPLSAVAGGTQNRYQITPTNQHPPQLPAYFATTGPGGAVPPNHLPFATQRH 1228
                     :|.|:...:..:|...|.:|...           :.:||...|.|..|......:.
  Fly  1055 ---------QNRYATKPNQSSGSNNNNHQGND-----------YQSTGMYSAPPSAHYGCLEPKQ 1099

  Fly  1229 AASEYQAGLNAARCAQSRACNSCDALATPSPMQPPPPVPVPEGWYQPVHPNSHPMHPTSSNHQIY 1293
            ...:.|..|.....|.:       |:.|.||.: ...:.:.|.....:...|...:|.:...|:.
  Fly  1100 QQQQQQPNLMTTSTAST-------AILTNSPAK-VKKINITENKMDQLEGKSERTNPFNQQQQLL 1156

  Fly  1294 QCSSECSDHSRSSQSHKRQLQLEEHGSSAKQRGGHHRRRAPVVQPCMESENENMLAEYEQRQYTS 1358
            ..|:.......:..:.....|:...|.:...|    |:|.|  :...:||| |:|.:...||.|
  Fly  1157 LASNALKQGLGAYANTTLAAQMASGGGAGTLR----RQRQP--KTLYKSEN-NILGKSGLRQNT- 1213

  Fly  1359 DCCNSSREGDTCSCSEGSCLYAEAGEPAPRQM 1390
                               :.|..|.|:..||
  Fly  1214 -------------------MNASVGAPSNGQM 1226

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo1	NP_476899.1	Ig	56..151	CDD:299845	50/94 (53%)
		I-set	56..150	CDD:254352	49/93 (53%)
		I-set	157..251	CDD:254352	54/93 (58%)
		Ig2_Robo	159..251	CDD:143201	52/91 (57%)
		I-set	255..341	CDD:254352	41/87 (47%)
		Ig3_Robo	272..341	CDD:143202	34/70 (49%)
		IG_like	351..436	CDD:214653	30/90 (33%)
		Ig	362..444	CDD:299845	28/87 (32%)
		I-set	445..531	CDD:254352	40/86 (47%)
		IGc2	459..521	CDD:197706	27/62 (44%)
		FN3	549..637	CDD:238020	33/87 (38%)
		FN3	673..762	CDD:238020	28/90 (31%)
		fn3	770..855	CDD:278470	31/88 (35%)
robo3	NP_608592.2	Ig	22..117	CDD:299845	50/94 (53%)
		I-set	23..116	CDD:254352	49/93 (53%)
		I-set	122..211	CDD:254352	54/94 (57%)
		Ig	125..211	CDD:299845	52/91 (57%)
		I-set	215..302	CDD:254352	41/87 (47%)
		Ig	232..302	CDD:299845	34/70 (49%)
		I-set	306..401	CDD:254352	32/94 (34%)
		Ig	320..411	CDD:299845	28/90 (31%)
		I-set	412..492	CDD:254352	38/79 (48%)
		IGc2	429..489	CDD:197706	26/59 (44%)
		FN3	519..612	CDD:238020	36/94 (38%)
		FN3	644..735	CDD:238020	31/95 (33%)
		fn3	743..832	CDD:278470	31/88 (35%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6635
eggNOG	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0.917438	Normalized mean entropy	S5483
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D173520at2759
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	1	1.000	-	-		mtm6572
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X493
	8	7.770