DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo1 and Robo3

DIOPT Version :9

Sequence 1:	NP_476899.1	Gene:	robo1 / 37603	FlyBaseID:	FBgn0005631	Length:	1395	Species:	Drosophila melanogaster
Sequence 2:	XP_038937330.1	Gene:	Robo3 / 315564	RGDID:	1311018	Length:	1451	Species:	Rattus norvegicus

Alignment Length:	1503	Identity:	433/1503 - (28%)
Similarity:	619/1503 - (41%)	Gaps:	276/1503 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly    20 NPSRSRSSRMWLLPAWLLLVLVASNG---LPAVRG-----QYQSPRIIEHPTDLVVKKNEPATLN 76
            |||        |||.....:.||..|   ||...|     :...|||:|.|.||:|.:.|||||.
  Rat    41 NPS--------LLPPGDPSLNVALPGHFELPPSPGSRVGPEDAMPRIVEQPPDLLVSRGEPATLP 97

  Fly    77 CKVEGKPEPTIEWFKDGEPVST--NEKKSHRVQFKDGALFFYRTMQGKKEQ-DGGEYWCVAKNRV 138
            |:.||:|.|.|||:|:|..|:|  .:.::||:....|||||.|.:.|::.: |.|.|.|||:|.:
  Rat    98 CRAEGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGALFFPRIVHGRRSRPDEGVYTCVARNYL 162

  Fly   139 GQAVSRHASLQIAVLRDDFRVEPKDTRVAKGETALLECGPPKGIPEPTLIWIKDGVPLDDLKAMS 203
            |.|.||:|||::||||||||..|.:..||.||.|::||.||||.|||.:.|.|....|.:
  Rat   163 GAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPKGHPEPLVTWKKGNTKLKE----- 222

  Fly   204 FGASSRVRIVDGGNLLISNVEPIDEGNYKCIAQNLVGTRESSYAKLIVQVKPYFMKEPKDQVMLY 268
              ...|:.| .||.|::|:....|.|.|.|:|.|:.|.|||..|:|:|..:|.|::.|.:||:|.
  Rat   223 --EEGRITI-RGGKLMMSHTFKSDAGMYMCVASNMAGERESGAAELVVLERPSFLRRPINQVVLA 284

  Fly   269 GQTATFHCSVGGDPPPKVLWKKEEGNIPVSRARILHDEKSLEISNITPTDEGTYVCEAHNNVGQI 333
            .....|.|.|.|||.|.:.|:|::|.:|..|..|..|. ||.|..::..|||||.|.|.|:||:.
  Rat   285 DAPVNFLCEVQGDPQPNLRWRKDDGELPTGRYEIRSDH-SLWIEQVSAEDEGTYTCVAENSVGRA 348

  Fly   334 SARASLIVHAPPNFTKRPSNKKVGLNGVVQLPCMASGNPPPSVFWTKEGVSTLMFPNSS---HGR 395
            .|..||.||.||.|..:|.|:.......|...|...|||||::||.|||...|:||:.|   .||
  Rat   349 EASGSLSVHVPPQFVTKPQNQTAAPGENVSFQCETKGNPPPAIFWQKEGSQVLLFPSQSLQPMGR 413

  Fly   396 QHVAADGTLQITDVRQEDEGYYVCSAFSVVDSSTVRVFLQV--SSLDERPPPIIQIGPANQTLPK 458
            ..|:..|.|.||:|:..|.|||||.|.||..|...:..|::  :|:|..||.|:| |||||||..
  Rat   414 LLVSPRGQLNITEVKIGDGGYYVCQAVSVAGSILAKALLEIKGASIDGLPPIILQ-GPANQTLVL 477

  Fly   459 GSVATLPCRATGNPSPRIKWFHDGHAVQAGN-RYSIIQGSSLRVDDLQLSDSGTYTCTASGERGE 522
            ||...||||..|||.|.::|..|...:|..| :::::...:|.:..:|..|.|.|:|.|....||
  Rat   478 GSSVWLPCRVIGNPQPNVQWKKDETWLQGDNSQFNLMDNGTLYIASVQEMDMGFYSCVAKSSIGE 542

  Fly   523 TSWAATLTVEKPGSTSLHRAADPSTYPAPPGTPKVLNVSRTSISLRWAKSQEKPGAVGPIIGYTV 587
            .:|.:.|...:....|...|.:|...|.||..|.|..|:..||:|.| |...:.||  ....|.:
  Rat   543 ATWNSWLRKREHWGASPGPATEPGNPPGPPSQPIVTEVTTNSITLTW-KPNPQSGA--RATSYVI 604

  Fly   588 EYFSPDLQTGWIVAAQRVGDTQVTISGLTPGTSYVFLVRAENTQGISVPSGLSNTIKTIEADFDA 652
            |.||......|...|..|.....|:|||.|.|.|:|||||....|:|.||.:|..::|
  Rat   605 EAFSQAAGNTWRTVADGVQLETHTVSGLQPNTIYLFLVRAVGAWGLSEPSPVSEPVQT------- 662

  Fly   653 ASANDLSAARTL---------LTGKSVELIDASAINASAVRLEWMLHVSADEKYVEGLRIHYKDA 708
               .|.|.:|.:         |...:|.:.:...:....:::.|.  |....:.|:|.|:.::.|
  Rat   663 ---QDSSLSRPVEDPWKGQQGLAEVAVRMQEPIVLGPRTLQVSWT--VEGPGQLVQGFRVSWRIA 722

  Fly   709 SVPSAQYHSITVMDASAESFVVGNLKKYTKYEFFLTPFFETIEGQ-----PSNSKTALTYEDVPS 768
            .:....:.::.|.....:|.|:..|...::.:..:     .::||     .|.|.|....|:.||
  Rat   723 GLDQGSWTALDVQSPHKQSTVLRGLPPGSQIQIKV-----QVQGQEGLGAESPSVTRSIPEEAPS 782

  Fly   769 APPDNIQI--GMYNQTAGWVRWTPPPSQHHNGNLYGYKIEVSAGNTMKVLANMTLNATTTSVLLN 831
            .||..:.:  |....::..|.|.||.....||.:..|:| ...||..:...|.:......||:..
  Rat   783 GPPQGVAVALGGDRNSSITVSWEPPLPSQQNGVITEYQI-WCLGNESRFHLNRSAAGWARSVMFR 846

  Fly   832 NLTTGAVYSVRLNSFTKAGDGPYSKPISLFMDPTHHVHPPRAHPSGTHDGRHEGQDLTYHNNGNI 896
            .|..|.:|...:.:.|.||.|..|.|:.:.:.     .||.|.|.
  Rat   847 GLLPGLLYRAIVAAATSAGVGVASAPVLVQLP-----FPPVAEPG-------------------- 886

  Fly   897 PPGDINPTTHKKTTDYLSGPWLMV---LVCIVLLVLVISAAISMVYFKRKHQMTKELGHLS---- 954
              .:::....::.:..|..|..:.   ..|..|| |.:.||:     .|:.:..|||.|.:
  Rat   887 --PEVSEGLAERLSRVLRKPAFLAGSSAACGALL-LGLCAAL-----YRRQKQRKELSHYTASFA 943

  Fly   955 -VVSDNEITALNINS------------KESLWIDHHRGWRTADTDKDSGLSESKL------LSHV 1000
             ..:...:.:..:.:            ..|:...|..|...:.:....||..:..      ..|.
  Rat   944 YTPAGKRLYSSGLRTPIPALLLPLSPLSSSVSFPHSEGLSGSSSRPPMGLGPAAYPWLADSWPHP 1008

  Fly  1001 NSSQS-----------------NYNNSDGGTDYAEVDTRNLTTFYNCRKSPDNPTPYATTMIIGT 1048
            :.|.|                 .|.|..|.:.|.....|.       ..:|.....|:|...:|
  Rat  1009 SRSPSAQEPRGSCCPSNPDPDDRYYNEAGISLYLAQTARG-------ANAPGEGPVYSTIDPVG- 1065

  Fly  1049 SSSETCTKTTSISADKDSGTHSPY---------SDAFAGQVPAVPVVKSNYLQYPVE--PINWSE 1102
            ...:|........:..|..|.|.|         |.|..|        |...|..||:  .::|.|
  Rat  1066 EELQTFHGGFPQHSSGDPSTWSQYAPPEWSEGDSGARGG--------KGKLLGKPVQMPSLSWPE 1122

  Fly  1103 FLPPPPEHPPPSSTYGYAQGSPESSRKSSKSAGSGISTNQSILNASIHSSSSGGFSAWGVSPQYA 1167
            .|||    ||||......:| ||...|.|..............:. |.|.|||   |..|:|...
  Rat  1123 ALPP----PPPSCELSCPEG-PEEELKGSSDLEEWCPPMPEKKHL-IDSRSSG---ACMVAPARG 1178

  Fly  1168 VACPPENVYSNPLSAVAGGTQNRYQITPTNQHPPQLPAYF---------ATTGPG---------- 1213
            ....|.:.|         |.|:...:||:...|||.|:..         ...||.
  Rat  1179 ETPSPTSSY---------GQQSTATLTPSPPDPPQPPSDIPHLHQMPRRVPLGPSSPLSVSQPAL 1234

  Fly  1214 ----------GAVP-------PNHLPFATQ------RHAASEYQAGLNAARCAQSRACNSCDALA 1255
                      ||.|       |:.:|....      |....|....|:..|....:...:.....
  Rat  1235 SSHDGRPVGLGAGPIVSYHPSPSPVPSTASSAPGRTRQVTGEMTPPLHGHRARIRKKTKALPHRR 1299

  Fly  1256 TPSPMQ-PPPPVPVPEGWYQPVHPNSHPMHPTSSNHQIYQCSSECSDHSRSSQSHKRQLQLEEHG 1319
            ..||.. ||||:|.||      ...|.|:.        .:.:...|...|.....:|..|....|
  Rat  1300 EHSPGDLPPPPLPPPE------EETSWPLG--------LRAAGSMSSLEREHSGERRVAQAVPLG 1350

  Fly  1320 SS--AKQRGGH----------HRRRAPVVQPCMESENENMLAEYEQRQYTSDC----CNSSREGD 1368
            :.  ..|||.|          .....|..:|...|..          |.||.|    .||||..:
  Rat  1351 AQPPGAQRGPHPDAALLGCAAEEAWLPYGRPSFLSHG----------QGTSTCSTAGSNSSRGSN 1405

  Fly  1369 TCSCSEGS 1376
            :...|.||
  Rat  1406 SSRGSRGS 1413

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo1	NP_476899.1	Ig	56..151	CDD:299845	48/97 (49%)
		I-set	56..150	CDD:254352	48/96 (50%)
		I-set	157..251	CDD:254352	38/93 (41%)
		Ig2_Robo	159..251	CDD:143201	36/91 (40%)
		I-set	255..341	CDD:254352	36/85 (42%)
		Ig3_Robo	272..341	CDD:143202	30/68 (44%)
		IG_like	351..436	CDD:214653	37/87 (43%)
		Ig	362..444	CDD:299845	37/86 (43%)
		I-set	445..531	CDD:254352	34/86 (40%)
		IGc2	459..521	CDD:197706	21/62 (34%)
		FN3	549..637	CDD:238020	34/87 (39%)
		FN3	673..762	CDD:238020	15/93 (16%)
		fn3	770..855	CDD:278470	23/86 (27%)
Robo3	XP_038937330.1	Ig strand A	182..185	CDD:409353	1/2 (50%)
		Ig strand A'	187..191	CDD:409353	0/3 (0%)
		Ig strand B	195..202	CDD:409353	3/6 (50%)
		Ig strand C	210..215	CDD:409353	1/4 (25%)
		Ig strand C'	217..220	CDD:409353	0/2 (0%)
		Ig strand D	226..230	CDD:409353	2/4 (50%)
		Ig strand E	232..238	CDD:409353	2/5 (40%)
		Ig strand F	245..253	CDD:409353	4/7 (57%)
		Ig strand G	256..267	CDD:409353	6/10 (60%)
		Ig	274..356	CDD:416386	34/82 (41%)
		Ig strand A	274..277	CDD:409353	0/2 (0%)
		Ig strand A'	279..283	CDD:409353	2/3 (67%)
		Ig strand B	286..295	CDD:409353	2/8 (25%)
		Ig strand C	301..306	CDD:409353	1/4 (25%)
		Ig strand C'	309..312	CDD:409353	1/2 (50%)
		Ig strand D	315..320	CDD:409353	2/4 (50%)
		Ig strand E	321..328	CDD:409353	4/7 (57%)
		Ig strand F	335..343	CDD:409353	5/7 (71%)
		Ig strand G	346..356	CDD:409353	4/9 (44%)
		Ig	361..458	CDD:416386	38/96 (40%)
		Ig strand A	361..365	CDD:409353	1/3 (33%)
		Ig strand A'	367..371	CDD:409353	1/3 (33%)
		Ig strand B	377..385	CDD:409353	2/7 (29%)
		Ig strand C	388..395	CDD:409353	4/6 (67%)
		Ig strand C'	397..400	CDD:409353	1/2 (50%)
		Ig strand D	413..418	CDD:409353	2/4 (50%)
		Ig strand E	420..427	CDD:409353	4/6 (67%)
		Ig strand F	433..442	CDD:409353	6/8 (75%)
		Ig strand G	444..455	CDD:409353	2/10 (20%)
		Ig	465..549	CDD:416386	33/84 (39%)
		Ig strand A	465..468	CDD:409353	1/2 (50%)
		Ig strand A'	472..475	CDD:409353	2/2 (100%)
		Ig strand B	481..488	CDD:409353	4/6 (67%)
		Ig strand C	494..499	CDD:409353	1/4 (25%)
		Ig strand C'	501..503	CDD:409353	0/1 (0%)
		Ig strand D	510..514	CDD:409353	0/3 (0%)
		Ig strand E	517..522	CDD:409353	1/4 (25%)
		Ig strand F	530..538	CDD:409353	4/7 (57%)
		Ig strand G	541..549	CDD:409353	3/7 (43%)
		FN3	569..662	CDD:238020	37/95 (39%)
		FN3	693..773	CDD:238020	14/86 (16%)
		FN3	783..876	CDD:238020	25/93 (27%)
		Ig	77..175	CDD:416386	48/97 (49%)
		Ig strand A	77..81	CDD:409353	3/3 (100%)
		Ig strand A'	84..89	CDD:409353	2/4 (50%)
		Ig strand B	93..101	CDD:409353	5/7 (71%)
		Ig strand C	107..112	CDD:409353	3/4 (75%)
		Ig strand C'	115..117	CDD:409353	0/1 (0%)
		Ig strand D	128..131	CDD:409353	1/2 (50%)
		Ig strand E	134..140	CDD:409353	5/5 (100%)
		Ig strand F	152..160	CDD:409353	5/7 (71%)
		Ig strand G	163..175	CDD:409353	7/11 (64%)
		Ig	182..267	CDD:416386	37/92 (40%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	106	1.000	Domainoid score	I6432
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	600	1.000	Inparanoid score	I913
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D173520at2759
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	1	1.000	-	-		mtm9043
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	1	1.100	-	-	O	PTHR12231
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	11	10.970