DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo1 and robo3

DIOPT Version :9

Sequence 1:	NP_476899.1	Gene:	robo1 / 37603	FlyBaseID:	FBgn0005631	Length:	1395	Species:	Drosophila melanogaster
Sequence 2:	XP_005172693.1	Gene:	robo3 / 30770	ZFINID:	ZDB-GENE-000209-4	Length:	1424	Species:	Danio rerio

Alignment Length:	1465	Identity:	458/1465 - (31%)
Similarity:	683/1465 - (46%)	Gaps:	232/1465 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly    45 GLPAVRGQYQ--SPRIIEHPTDLVVKKNEPATLNCKVEGKPEPTIEWFKDGEPVSTN--EKKSHR 105
            ||...|.:.:  :|||:|||:||:|.|.||||||||.||:|.|.:||:||||.|.|:  :.:|||
Zfish    50 GLNGSRPRLEDAAPRIVEHPSDLIVSKGEPATLNCKAEGRPTPMVEWYKDGERVETDREDPRSHR 114

  Fly   106 VQFKDGALFFYRTMQGKKEQ-DGGEYWCVAKNRVGQAVSRHASLQIAVLRDDFRVEPKDTRVAKG 169
            :....|:|||.|.:.|::.: |.|.|.|||:|.:|:||||:|||::|:||||||..|.|..||.|
Zfish   115 MLLPSGSLFFLRIVHGRRSKPDEGVYVCVARNYLGEAVSRNASLEVAILRDDFRQTPSDVVVAAG 179

  Fly   170 ETALLECGPPKGIPEPTLIWIKDGVPLDDLKAMSFGASSRVRIVDGGNLLISNVEPIDEGNYKCI 234
            |.|::||.||:|.||||:.|.::.|.::|       ...|:.| .||.|:|||....|.|.|.|:
Zfish   180 EPAVMECIPPRGHPEPTISWKRNNVKVND-------KDERITI-RGGKLMISNTRKSDAGMYVCV 236

  Fly   235 AQNLVGTRESSYAKLIVQVKPYFMKEPKDQVMLYGQTATFHCSVGGDPPPKVLWKKEEGNIPVSR 299
            ..|:||.::|..|:|:|..:|.|::.|.:||:|...|..|.|.|.|||.|.:.||||||.:|..|
Zfish   237 GTNMVGEKDSDPAELVVFERPMFVRRPVNQVVLADDTVDFQCEVQGDPAPTIRWKKEEGELPRGR 301

  Fly   300 ARILHDEKSLEISNITPTDEGTYVCEAHNNVGQISARASLIVHA----PPNFTKRPSNKKVGLNG 360
            ..|..| .||.::.:...|||:|.|.:.|:||:..|..||.||.    ||....||.::......
Zfish   302 FEIRAD-NSLRLTQVKAEDEGSYTCLSENSVGKAEASGSLQVHVGPSLPPQIVVRPRDQISAQGR 365

  Fly   361 VVQLPCMASGNPPPSVFWTKEGVSTLMFPN---SSHGRQHVAADGTLQITDVRQEDEGYYVCSAF 422
            .|...|...|||||:|||.|||...|:||:   |..||..|:..|.|.||||..||.|||:|.|.
Zfish   366 TVTFLCGTKGNPPPAVFWQKEGSQVLLFPSQPPSQSGRFSVSLSGELTITDVHSEDSGYYICQAI 430

  Fly   423 SVVDSSTVRVFLQVSSL-DERPPPIIQIGPANQTLPKGSVATLPCRATGNPSPRIKWFHDGHAVQ 486
            ||..|...:..|:|.|. .:|.||||:.|||||||..|:.|.|.|...|||.|.|:|..||..:.
Zfish   431 SVAGSILTKALLEVESTPSDRIPPIIRQGPANQTLAPGTTAQLQCHVMGNPLPSIQWERDGQRIL 495

  Fly   487 A-GNRYSIIQGSSLRVDDLQLSDSGTYTCTASGERGETSWAATLTVEKPGSTSLHRAADPSTYPA 550
            . ..|.|:::..:|::..||.:|||.|||.||...|||||:..|||::.|..|....::|...|.
Zfish   496 GIDERISLMENGTLQITALQETDSGAYTCVASSLSGETSWSGVLTVKESGGLSASPVSEPYQLPG 560

  Fly   551 PPGTPKVLNVSRTSISLRWAKSQEKPGAVGPIIGYTVEYFSPDLQTGWIVAAQRVGDTQVTISGL 615
            ||..|.|.:|:|.|::|.|..:..:.||.  :..|.:|.||....:.|...|..|...:.|::||
Zfish   561 PPQKPVVTDVTRNSVTLTWQPNAHEGGAA--VTSYIIEAFSQSAGSTWQTVADFVKLEKHTVTGL 623

  Fly   616 TPGTSYVFLVRAENTQGISVPSGLSNTIKTIEADFDAASANDLSAARTLLTGK-SVELIDASAIN 679
            :|.|.|:|:|||.|..|:|.||.:|..::|.:........:.....|.|  |: ||:|.:...:.
Zfish   624 SPNTIYLFIVRAVNAYGLSDPSPISEPVRTQDVSPTGQGVDHRQVQREL--GEMSVQLHEPVLLT 686

  Fly   680 ASAVRLEWMLHVSADEKYVEGLRIHYKDASVPSAQYHSITVMDASAESFVVGNLKKYTKYEFFLT 744
            ||:|::.|  :|....:|::|.|:.|:......:|..   |..|:..|.|:.||.|.|:||..:.
Zfish   687 ASSVKISW--NVDRQSQYIQGYRLLYRPVGGSWSQQE---VKGATERSAVIANLLKGTEYEIKIR 746

  Fly   745 PFFETIEGQPSNSKTALTYEDVPSAPPDNIQIG---MYNQTAGWVRWTPPPSQHHNGNLYGYKIE 806
            |:|...:|..|.|:|..|.|:||||||..:.:.   :.|.::..|.|.|||:...||.:..||:.
Zfish   747 PYFNEFQGLDSRSQTLRTPEEVPSAPPRAVNVATVKLSNSSSISVSWQPPPTDMQNGVIQEYKVW 811

  Fly   807 VSAGNTM-KVLANMTLNATTTSVLLNNLTTGAVYSVRLNSFTKAGDGPYSKPISLFMDPTHHVHP 870
            ....::. :...|.|::.:..|.:|..|..|.:|.|.:.:.|.||.|.:|:|:|:.      :..
Zfish   812 CLGNDSQTRYNINQTVDGSVLSTVLKGLLPGVLYQVEVAAVTSAGVGKHSQPVSVL------IKL 870

  Fly   871 PRAHPSGTHDGRHEGQDLTYHNNGNIPPGDINPTTHKKTTDYLSGPWLMVLVCIVLLVLVISAAI 935
            |...|:.|..|....:               |.:..::.||.:..|..:..:.....|:::..::
Zfish   871 PAEQPTVTGGGSESEE---------------NVSLAEQITDVVKQPAFIAGIGGACWVILMGFSV 920

  Fly   936 SMVYFKRKHQMTKELGHLSV---------VSDNEITALN-------INSKESLWIDHHRGWRTAD 984
             .:|.:||.:  |||.|.:.         ....|.:.||       .|.....|:..  .|.|.:
Zfish   921 -WIYCRRKKR--KELSHYTASFAYTPAVGFPHGESSGLNGRPGMLGANMGNYPWLAD--SWPTTN 980

  Fly   985 ---TDKDS-------------GLSESKLLSHVNSSQSNYNNSDGGTDYAEV-----DTRNLTTFY 1028
               ..|||             ..:|:.:.:::|.::....:|:.|..|:.:     |.|:.|..|
Zfish   981 LVHNGKDSVNCCTSKHETPERYYNEAGISNYLNQNEKYSADSNEGPIYSTIDPTSEDLRSFTAQY 1045

  Fly  1029 NCRKSPDNPTPYATTMIIGTSSSETCTKTTSISADKDSGTH---------SPYSDAFAGQVPAVP 1084
            :     .:.||||:|.|:..::...|      |.::| |:|         :.|:.....:...|.
Zfish  1046 S-----QHTTPYASTPIMPFTNQAPC------SPEQD-GSHWMTQPGTSNAQYAQPDRSKTENVK 1098

  Fly  1085 VVKSNYLQYPVE--PINWSEFLPPPPEH--------------------------PP-PSSTY--- 1117
            ..|...:..||:  .:||.:.|||||..                          || |..:|
Zfish  1099 QPKQKLMGKPVKTPSLNWMDALPPPPISGDLEQCDEEDDNELIQDMGSDEEEWCPPLPERSYLMD 1163

  Fly  1118 GYAQGSPESSRKSSKSAGSGISTNQSILNASIHSSSSGGFS-----------------AWGVSPQ 1165
            |..:..|....:...|:.:...::||....:......|.|.                 ..|..|.
Zfish  1164 GMVEDGPVPPPRGEASSPTTSYSHQSTATLTPSPRDDGHFHDIPRLHQLEQPHMTRRLPCGPVPV 1228

  Fly  1166 YAVACPPENVYSNP-LSAVAGGTQNRYQITPTNQHP-------------PQLPAYFATTGPGGAV 1216
            .....|| ...||| |||        .|..|.|..|             .|.....:.|.||.|.
Zfish  1229 PRAPSPP-LTQSNPNLSA--------NQECPDNTVPHRSAHKRDLSQGAAQSVENISNTTPGRAR 1284

  Fly  1217 PPNHLPFATQRHAASEYQAGL--NAARCAQSRACNSCDALATPSPMQPPPPVPVPEGWYQPVHPN 1279
            |       :..|.:|..::.:  .|::.||.|.    |.|...   .||||.|.||......|..
Zfish  1285 P-------SADHTSSGQKSKVKKKASKSAQYRR----DILQGD---LPPPPEPPPEDQDNCAHVG 1335

  Fly  1280 SHPMHPTSSNHQIYQCSSECSDHSRSSQSHKRQLQLEEHGSSAKQRGGHHRRRAPVVQPCMESEN 1344
            .            ::|.....:.:.||.|     .:|....|...|.....||.......:....
Zfish  1336 G------------FECGQTSLERAGSSNS-----SMERREQSTPNRKNSAHRRIDADDDLIPYNK 1383

  Fly  1345 ENMLAEYEQRQYTSDCC---NSSREGDTCSCSEGS 1376
            .|.|:   :.|.:|:|.   :||..|.|.|..:|:
Zfish  1384 PNFLS---RGQMSSNCSTTGSSSSRGSTGSRGQGT 1415

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo1	NP_476899.1	Ig	56..151	CDD:299845	54/97 (56%)
		I-set	56..150	CDD:254352	54/96 (56%)
		I-set	157..251	CDD:254352	39/93 (42%)
		Ig2_Robo	159..251	CDD:143201	37/91 (41%)
		I-set	255..341	CDD:254352	37/85 (44%)
		Ig3_Robo	272..341	CDD:143202	30/68 (44%)
		IG_like	351..436	CDD:214653	38/87 (44%)
		Ig	362..444	CDD:299845	40/85 (47%)
		I-set	445..531	CDD:254352	41/86 (48%)
		IGc2	459..521	CDD:197706	25/62 (40%)
		FN3	549..637	CDD:238020	32/87 (37%)
		FN3	673..762	CDD:238020	26/88 (30%)
		fn3	770..855	CDD:278470	24/88 (27%)
robo3	XP_005172693.1	Ig1_Robo	62..161	CDD:143317	54/98 (55%)
		I-set	63..160	CDD:254352	54/96 (56%)
		I-set	167..253	CDD:254352	39/93 (42%)
		Ig	169..253	CDD:299845	37/91 (41%)
		I-set	257..342	CDD:254352	37/85 (44%)
		Ig3_Robo	274..342	CDD:143202	30/68 (44%)
		IG_like	349..444	CDD:214653	41/94 (44%)
		Ig	364..453	CDD:299845	40/88 (45%)
		IG_like	460..541	CDD:214653	37/80 (46%)
		IGc2	467..528	CDD:197706	24/60 (40%)
		FN3	559..653	CDD:238020	35/95 (37%)
		FN3	685..764	CDD:238020	26/83 (31%)
		FN3	<793..866	CDD:238020	22/72 (31%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6635
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	622	1.000	Inparanoid score	I848
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D173520at2759
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	1	1.000	-	-		mtm6572
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3358
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	11	10.900