DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo1 and Robo2

DIOPT Version :9

Sequence 1:	NP_476899.1	Gene:	robo1 / 37603	FlyBaseID:	FBgn0005631	Length:	1395	Species:	Drosophila melanogaster
Sequence 2:	XP_006523098.1	Gene:	Robo2 / 268902	MGIID:	1890110	Length:	1598	Species:	Mus musculus

Alignment Length:	1447	Identity:	445/1447 - (30%)
Similarity:	658/1447 - (45%)	Gaps:	263/1447 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly    19 NNPSRSRSSRM-WLLPAWLLLVLVASNGLPAVRGQYQSPRIIEHPTDLVVKKNEPATLNCKVEGK 82
            :.|:..:.:|. |....|        .|...:|.:...|||:|||:|::|.|.||.|||||.||:
Mouse    24 SGPTLGKLTRTPWKGKPW--------KGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGR 80

  Fly    83 PEPTIEWFKDGEPVST--NEKKSHRVQFKDGALFFYRTMQGKKEQ-DGGEYWCVAKNRVGQAVSR 144
            |.|||||:||||.|.|  ::.:|||:....|:|||.|.:.|::.: |.|.|.|||:|.:|:||||
Mouse    81 PTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSR 145

  Fly   145 HASLQIAVLRDDFRVEPKDTRVAKGETALLECGPPKGIPEPTLIWIKDGVPLDDLKAMSFGASSR 209
            :|||::|:||||||..|.|..||.||.|:|||.||:|.||||:.|.||.|.:||       ...|
Mouse   146 NASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDD-------KEER 203

  Fly   210 VRIVDGGNLLISNVEPIDEGNYKCIAQNLVGTRESSYAKLIVQVKPYFMKEPKDQVMLYGQTATF 274
            :.| .||.|:|||....|.|.|.|:..|:||.|:|..|:|.|..:|.|::.|.:||:|..:...|
Mouse   204 ISI-RGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEF 267

  Fly   275 HCSVGGDPPPKVLWKKEEGNIPVSRARILHDEKSLEISNITPTDEGTYVCEAHNNVGQISARASL 339
            .|.|.|||.|.|.|||::.::|..|..| .|:.:|.|.....||||||||.|.|.||::.|.|:|
Mouse   268 RCQVQGDPQPTVRWKKDDADLPRGRYDI-KDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATL 331

  Fly   340 IVH----APPNFTKRPSNKKVGLNGVVQLPCMASGNPPPSVFWTKEGVSTLMFPN---SSHGRQH 397
            .|.    |||.|..||.::.|.....|..||...|||.|:|||.|||...|:|||   ..:.|..
Mouse   332 TVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCS 396

  Fly   398 VAADGTLQITDVRQEDEGYYVCSAFSVVDSSTVRVFLQVSS-LDERPPPIIQIGPANQTLPKGSV 461
            |:..|.|.||::::.|.|||:|.|.:|..|...:..|:|:. |.:||||||..||.||||.....
Mouse   397 VSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGT 461

  Fly   462 ATLPCRATGNPSPRIKWFHDGHAVQAGNRYSIIQG-SSLRVDDLQLSDSGTYTCTASGERGETSW 525
            |.|.|:|||.|.|.|.|..:|......:..:.||. .:|::.:|::||:|||||.|:...|||||
Mouse   462 ALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSW 526

  Fly   526 AATLTVEKPGSTSLHRAADPSTYPAPPGTPKVLNVSRTSISLRWAKSQEKPGAVG--PIIGYTVE 588
            :|.|.|.:.|:| :.:..|.:..|.||..|:|.:||:.|::|.|     :||..|  |...|.:|
Mouse   527 SAVLDVTESGAT-ISKNYDMNDLPGPPSKPQVTDVSKNSVTLSW-----QPGTPGVLPASAYIIE 585

  Fly   589 YFSPDLQTGWIVAAQRVGDTQVTISGLTPGTSYVFLVRAENTQGISVPSGLSNTIKTIEADFDAA 653
            .||..:...|...|..|..|..|:.||.|.|.|:|:|||.|.||:|.||.:|:.::|.:.. ..|
Mouse   586 AFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDIS-PPA 649

  Fly   654 SANDLSAARTLLTGKSVELIDASAINASAVRLEWMLHVSADEKYVEGLRIHYKDAS--VPSAQYH 716
            ...|....:..|....|.|.:...:..:.|::.|.  |....::::|.|:.|:..|  ..|..:.
Mouse   650 QGVDHRQVQKELGDVVVRLHNPVVLTPTTVQVTWT--VDRQPQFIQGYRVMYRQTSGLQASTVWQ 712

  Fly   717 SITVMDASAESFVVGNLKKYTKYEFFLTPFFETIEGQPSNSKTALTYEDVPSAPPDNI---QIGM 778
            ::.....:..|.|:.||||...||..:.|:|...:|..|.|||..|.|:.|||||.::   .:|.
Mouse   713 NLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGS 777

  Fly   779 YNQTAGWVRWTPPPSQHHNGNLYGYKIEVSAGNTMKVLANMTLNATTTSVLLNNLTTGAVYSVRL 843
            :|.|:..|.|.|||:.|.||.:..||| ...||..:...|.|::|...||::..|..|..|.|.:
Mouse   778 HNSTSISVSWDPPPADHQNGIIQEYKI-WCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEV 841

  Fly   844 NSFTKAGDGPYSKPISLFMDPTHHVHPPRAHPSGTHDGRHEGQDLTYHNNGNIPPGDINPTTHKK 908
            .:.|.||.|..|:|..:.:                 .||:| ..:|.:||          :..::
Mouse   842 AASTSAGVGVKSEPQPIII-----------------GGRNE-VVITENNN----------SITEQ 878

  Fly   909 TTDYLSGPWLMVLVCIVLLVLVISAAISMVYFKRK-------HQMTKELGHLSVVSDNEITA-LN 965
            .||.:..|..:..:.....|:::..:| .:|::||       :.:|.:.|...::|:..... ||
Mouse   879 ITDVVKQPAFIAGIGGACWVILMGFSI-WLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLN 942

  Fly   966 INSKESLWIDHHRGWRTADTDKDSGLSESKLLSHVNSSQSNYNN--------------------- 1009
            .......|:            .||..:.|..:::.||..:...|
Mouse   943 AGDPNYPWL------------ADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATML 995

  Fly  1010 SDGGTDYAEVDTRNLTTFYNCRKSPDNPTPYATTMIIGTSS------------------------ 1050
            |||.. |:.:|....|| ||........||||||.|:.::|
Mouse   996 SDGAI-YSSIDFTTKTT-YNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKTDLM 1058

  Fly  1051 ------------------------------------SETCTKTTSISADKDSGTHSPYSDAFAGQ 1079
                                                |:..:.|:|.::.:.||:.|      .|:
Mouse  1059 GFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLS------GGK 1117

  Fly  1080 VPAVPVVKSNYLQYPVEPINWSEF-LPPPPEHPPPSSTYGYAQGSPESSRKSSKSAGSGISTNQS 1143
            .......|::..........|:.. |||||..|.|.:..|:.....|          :|..::..
Mouse  1118 GGKKKKTKNSSKAQKNNGSTWANVPLPPPPVQPLPGTELGHYAAEQE----------NGYDSDSW 1172

  Fly  1144 ILNASIHSSSSGGFSAWGVSPQYAVACPP-ENVYSNPLSAVAGGTQNRYQITPT--NQHPPQLPA 1205
            .....:.:....|........:..|..|| ..|.|:|  |::.|.|:...:||:  .:..|.|.|
Mouse  1173 CPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSP--AISFGQQSTATLTPSPREEMQPMLQA 1235

  Fly  1206 YFATTGPGGAVPPNHLPFATQRHAASEYQAGLNAARCAQSRACNSCDALATPSPMQPPPPV---- 1266
                          ||...|:     .||  .:.|:.......|      ||.|..|.||:
Mouse  1236 --------------HLDELTR-----AYQ--FDIAKQTWHIQSN------TPPPQPPAPPLGYVS 1273

  Fly  1267 ---------PVPEGWYQPVHPNSHPMH-----------PTSSNHQIYQCSS--------ECSDHS 1303
                     .||:   :.......|:.           |.||...:....:        ..||..
Mouse  1274 GALISDLETDVPD---EDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDED 1335

  Fly  1304 RSSQSHKRQLQLEEHGS 1320
            |:..||:..:.....||
Mouse  1336 RNFSSHRSSVGSSSDGS 1352

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo1	NP_476899.1	Ig	56..151	CDD:299845	54/97 (56%)
		I-set	56..150	CDD:254352	54/96 (56%)
		I-set	157..251	CDD:254352	44/93 (47%)
		Ig2_Robo	159..251	CDD:143201	42/91 (46%)
		I-set	255..341	CDD:254352	38/85 (45%)
		Ig3_Robo	272..341	CDD:143202	32/68 (47%)
		IG_like	351..436	CDD:214653	34/87 (39%)
		Ig	362..444	CDD:299845	35/85 (41%)
		I-set	445..531	CDD:254352	39/86 (45%)
		IGc2	459..521	CDD:197706	23/62 (37%)
		FN3	549..637	CDD:238020	36/89 (40%)
		FN3	673..762	CDD:238020	23/90 (26%)
		fn3	770..855	CDD:278470	31/87 (36%)
Robo2	XP_006523098.1	Ig1_Robo	53..152	CDD:143317	54/98 (55%)
		Ig	160..244	CDD:386229	42/91 (46%)
		Ig	265..333	CDD:386229	32/68 (47%)
		Ig4_Robo	355..444	CDD:143203	35/88 (40%)
		IG	451..532	CDD:214652	35/80 (44%)
		FN3	549..642	CDD:238020	39/97 (40%)
		FN3	673..758	CDD:238020	23/86 (27%)
		FN3	767..859	CDD:238020	32/92 (35%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6680
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H43188
Inparanoid	1	1.050	602	1.000	Inparanoid score	I935
Isobase	1	0.950	-	0.917438	Normalized mean entropy	S5483
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	1	1.000	-	-		mtm8796
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	1	1.100	-	-	O	PTHR12231
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3358
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	12	11.940