DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo1 and Robo1

DIOPT Version :9

Sequence 1:	NP_476899.1	Gene:	robo1 / 37603	FlyBaseID:	FBgn0005631	Length:	1395	Species:	Drosophila melanogaster
Sequence 2:	XP_017172382.1	Gene:	Robo1 / 19876	MGIID:	1274781	Length:	1687	Species:	Mus musculus

Alignment Length:	1462	Identity:	433/1462 - (29%)
Similarity:	632/1462 - (43%)	Gaps:	324/1462 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 HAIARSTSTTNNPSRSRSSRMWLLPAWLLLVLVAS----------NGLPA--------------- 48
            ||:....:|..:.:.|.:           |||..:          ||.||
Mouse    39 HAVIEMATTVTSVTPSAA-----------LVLFPTDPEDLERGNDNGTPAPTSDNDDNSLGYTGS 92

  Fly    49 -VRGQYQSPRIIEHPTDLVVKKNEPATLNCKVEGKPEPTIEWFKDGEPVST--NEKKSHRVQFKD 110
             :|.:...|||:|||:||:|.|.||||||||.||:|.|||||:|.||.|.|  ::.:|||:....
Mouse    93 RLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPS 157

  Fly   111 GALFFYRTMQGKKEQ-DGGEYWCVAKNRVGQAVSRHASLQIAVLRDDFRVEPKDTRVAKGETALL 174
            |:|||.|.:.|:|.: |.|.|.|||:|.:|:|||.:|||::|:||||||..|.|..||.||.|::
Mouse   158 GSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVM 222

  Fly   175 ECGPPKGIPEPTLIWIKDGVPLDDLKAMSFGASSRVRIVDGGNLLISNVEPIDEGNYKCIAQNLV 239
            ||.||:|.||||:.|.|||.||||       ...|:.| .||.|:|:.....|.|.|.|:..|:|
Mouse   223 ECQPPRGHPEPTISWKKDGSPLDD-------KDERITI-RGGKLMITYTRKSDAGKYVCVGTNMV 279

  Fly   240 GTRESSYAKLIVQVKPYFMKEPKDQVMLYGQTATFHCSVGGDPPPKVLWKKEEGNIPVSRARILH 304
            |.|||..|:|.|..:|.|:|.|.:..:....:|.|.|...|||.|.|.|:|::|.:|.||..| .
Mouse   280 GERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEI-R 343

  Fly   305 DEKSLEISNITPTDEGTYVCEAHNNVGQISARASLIVHA---PPNFTKRPSNKKVGLNGVVQLPC 366
            |:.:|:|..:|..|.|:|.|.|.|.||:..|.|:|.|..   ||:|..:|.::.|.|...|...|
Mouse   344 DDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQC 408

  Fly   367 MASGNPPPSVFWTKEGVSTLMF---PNSSHGRQHVAADGTLQITDVRQEDEGYYVCSAFSVVDSS 428
            .|:|||.|::||.:||...|:|   |..|..|..|:..|.|.||:|::.|.|||:|...:|..|.
Mouse   409 EATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSI 473

  Fly   429 TVRVFLQVSS-LDERPPPIIQIGPANQTLPKGSVATLPCRATGNPSPRIKWFHDGHAVQA-GNRY 491
            ..:.:|:|:. :.:||||:|:.||.|||:.......|.|.|||:|:|.|.|..||..|.. .:|.
Mouse   474 ITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRI 538

  Fly   492 SIIQGSSLRVDDLQLSDSGTYTCTASGERGETSWAATLTVEKPG-STSLHRAADPSTYPAPPGTP 555
            ..::...|::...:|.|:|.||||||...||.:|:|.:.|::.| .....|..||:..|:.|..|
Mouse   539 KQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKP 603

  Fly   556 KVLNVSRTSISLRWAKSQEKPGAVGPIIGYTVEYFSPDLQTGWIVAAQRVGDTQVTISGLTPGTS 620
            :|.:||:.:::|.|..:... ||..  ..|.:|.||....:.|..||:.|......|.||.|...
Mouse   604 EVTDVSKNTVTLSWQPNLNS-GATP--TSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAI 665

  Fly   621 YVFLVRAENTQGISVPSGLSNTIKTIEADFDAASANDLSAARTLLTGKSV-ELIDASAINASAVR 684
            |:|||||.|..|||.||.:|:.:||.:....:...:.....|.|  |..| .|.:.:.:::|:|.
Mouse   666 YLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQREL--GNVVLHLHNPTILSSSSVE 728

  Fly   685 LEWMLHVSADEKYVEGLRIHYKDASVPSAQYHS------ITVMDASAESFVVGNLKKYTKYEFFL 743
            :.|.  |....:|::|.:|.|:    ||...|.      ..|...:..|.|:.:|:|...||...
Mouse   729 VHWT--VDQQSQYIQGYKILYR----PSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKA 787

  Fly   744 TPFFETIEGQPSNSKTALTYEDVPSAPPDNIQIGMY--NQTAGWVRWTPPPSQHHNGNLYGYKIE 806
            .|||...:|..|..|.|.|.|:.|||||.::.:...  |.||..|.|.|||....||.:..||:
Mouse   788 RPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKV- 851

  Fly   807 VSAGNTMKVLANMTLNATTTSVLLNNLTTGAVYSVRLNSFTKAGDGPYSKPISLFMDPTHHVHPP 871
            ...||..|...|.|::.:|.||::.:|..|..|||.:.:.|.||.|..|:|..:.:|
Mouse   852 WCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLD-------- 908

  Fly   872 RAHPSGTHDGRHEGQDLTYHNNGN-IPPGDINPTTHKKTTDYLSGP----------WLMVLVCIV 925
                                ::|| :.|.| ..:..::.:|.:..|          |::::|..:
Mouse   909 --------------------SHGNPVSPED-QVSLAQQISDVVRQPAFIAGIGAACWIILMVFSI 952

  Fly   926 LLVLVISAAISMVYFKRKHQ---------------------MTKELGHLSVVSDNEITALNINSK 969
            .|           |..||.:                     :|.:.|..:|.|......|||:..
Mouse   953 WL-----------YRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEP 1006

  Fly   970 ESL-WI--------DHHRGWRTADTDKDSGLSESKLLSHVNSSQ--SNYNNS----------DGG 1013
            .:. |:        ::|...........:|.|:|.|.::...:.  :||||.          ...
Mouse  1007 ATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPES 1071

  Fly  1014 TDYAEVDTRNLTTFYNCRKSPD-----------NPTPYATTMII------------GTSS----- 1050
            |.|.:||..|.........||:           .|||||||.:|            |.||
Mouse  1072 TVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWK 1136

  Fly  1051 ----------------------------SETCTKTTSISADKDSGTHSPYSDAFAGQVPAVPVVK 1087
                                        ::|...|...:...|..|...|:.:..|...:.....
Mouse  1137 PPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGH 1201

  Fly  1088 SNYLQYPVEP----INWSEFLPPPPEHPPPSSTYGYAQGSPESSRKSSKSAGSGISTNQSILNAS 1148
            ....:.|..|    :||::.|||||.||||.|                         |....|.|
Mouse  1202 KKGARTPKAPKQGGMNWADLLPPPPAHPPPHS-------------------------NSEEYNMS 1241

  Fly  1149 IHSSSSGGFSAWGVSPQYAVACPPENVY------------SNPLSAVAGGTQNRYQITPTNQHPP 1201
            :..|         ...:.....||..:|            ..|...|.|...:...::.::|...
Mouse  1242 VDES---------YDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTA 1297

  Fly  1202 QLPAYFATTGPGGAVPP---------NHLPFATQRHAASEYQAGLNAARCAQSRACNSCDALATP 1257
            .|     |..|...:.|         .|:|....|..
Mouse  1298 TL-----TPSPQEELQPMLQDCPEDLGHMPHPPDRRR---------------------------- 1329

  Fly  1258 SPMQPPPPVPVPEGWYQPVHPNSHPMHPTSSN 1289
            .|:.|||| |.|   ..|.|...:...|..|:
Mouse  1330 QPVSPPPP-PRP---ISPPHTYGYISGPLVSD 1357

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo1	NP_476899.1	Ig	56..151	CDD:299845	55/97 (57%)
		I-set	56..150	CDD:254352	55/96 (57%)
		I-set	157..251	CDD:254352	44/93 (47%)
		Ig2_Robo	159..251	CDD:143201	42/91 (46%)
		I-set	255..341	CDD:254352	34/85 (40%)
		Ig3_Robo	272..341	CDD:143202	30/68 (44%)
		IG_like	351..436	CDD:214653	33/87 (38%)
		Ig	362..444	CDD:299845	32/85 (38%)
		I-set	445..531	CDD:254352	34/86 (40%)
		IGc2	459..521	CDD:197706	23/62 (37%)
		FN3	549..637	CDD:238020	32/87 (37%)
		FN3	673..762	CDD:238020	25/94 (27%)
		fn3	770..855	CDD:278470	31/86 (36%)
Robo1	XP_017172382.1	Ig1_Robo	100..199	CDD:143317	55/98 (56%)
		Ig2_Robo	207..291	CDD:143201	42/91 (46%)
		Ig3_Robo	312..380	CDD:143202	30/68 (44%)
		Ig4_Robo	401..490	CDD:143203	32/88 (36%)
		I-set	491..578	CDD:369462	34/86 (40%)
		FN3	597..683	CDD:238020	33/88 (38%)
		FN3	720..806	CDD:238020	25/91 (27%)
		fn3	814..897	CDD:365830	30/83 (36%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6680
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	602	1.000	Inparanoid score	I935
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	1	1.000	-	-		mtm8796
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	1	1.100	-	-	O	PTHR12231
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3358
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	10	9.990