DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo1 and Robo3

DIOPT Version :9

Sequence 1:	NP_476899.1	Gene:	robo1 / 37603	FlyBaseID:	FBgn0005631	Length:	1395	Species:	Drosophila melanogaster
Sequence 2:	XP_006510170.1	Gene:	Robo3 / 19649	MGIID:	1343102	Length:	1403	Species:	Mus musculus

Alignment Length:	1518	Identity:	437/1518 - (28%)
Similarity:	626/1518 - (41%)	Gaps:	311/1518 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly    10 AIARSTSTTN-------------NPSRSRSSRMWLLPAWLLLVLVASNGLPAVRG-----QYQSP 56
            ::||..|.::             |||        |||.          |.|::.|     :...|
Mouse    18 SLARDISNSSELLFGFNSSLAALNPS--------LLPP----------GDPSLNGSRVGPEDAMP 64

  Fly    57 RIIEHPTDLVVKKNEPATLNCKVEGKPEPTIEWFKDGEPVST--NEKKSHRVQFKDGALFFYRTM 119
            ||:|.|.||||.:.|||||.|:.||:|.|.|||:|:|..|:|  .:.::||:....|||||.|.:
Mouse    65 RIVEQPPDLVVSRGEPATLPCRAEGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGALFFPRIV 129

  Fly   120 QGKKEQ-DGGEYWCVAKNRVGQAVSRHASLQIAVLRDDFRVEPKDTRVAKGETALLECGPPKGIP 183
            .|::.: |.|.|.|||:|.:|.|.||:|||::||||||||..|.:..||.||.|::||.||||.|
Mouse   130 HGRRSRPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPKGHP 194

  Fly   184 EPTLIWIKDGVPLDDLKAMSFGASSRVRIVDGGNLLISNVEPIDEGNYKCIAQNLVGTRESSYAK 248
            ||.:.|.|..:.|.:       ...|:.| .||.|::|:....|.|.|.|:|.|:.|.|||..|:
Mouse   195 EPLVTWKKGKIKLKE-------EEGRITI-RGGKLMMSHTFKSDAGMYMCVASNMAGERESGAAE 251

  Fly   249 LIVQVKPYFMKEPKDQVMLYGQTATFHCSVGGDPPPKVLWKKEEGNIPVSRARILHDEKSLEISN 313
            |:|..:|.|::.|.:||:|......|.|.|.|||.|.:.|:|::|.:|..|..|..|. ||.|..
Mouse   252 LVVLERPSFLRRPINQVVLADAPVNFLCEVQGDPQPNLHWRKDDGELPAGRYEIRSDH-SLWIDQ 315

  Fly   314 ITPTDEGTYVCEAHNNVGQISARASLIVHAPPNFTKRPSNKKVGLNGVVQLPCMASGNPPPSVFW 378
            ::..|||||.|.|.|:||:..|..||.||.||.|..:|.|:.|.....|...|...|||||::||
Mouse   316 VSSEDEGTYTCVAENSVGRAEASGSLSVHVPPQFVTKPQNQTVAPGANVSFQCETKGNPPPAIFW 380

  Fly   379 TKEGVSTLMFPNSS---HGRQHVAADGTLQITDVRQEDEGYYVCSAFSVVDSSTVRVFLQV--SS 438
            .|||...|:||:.|   .||..|:..|.|.||:|:..|.|||||.|.||..|...:..|::  :|
Mouse   381 QKEGSQVLLFPSQSLQPMGRLLVSPRGQLNITEVKIGDGGYYVCQAVSVAGSILAKALLEIKGAS 445

  Fly   439 LDERPPPIIQIGPANQTLPKGSVATLPCRATGNPSPRIKWFHDGHAVQA-GNRYSIIQGSSLRVD 502
            :|..||.|:| |||||||..||...||||..|||.|.|:|..|...:|. .::::::...:|.:.
Mouse   446 IDGLPPIILQ-GPANQTLVLGSSVWLPCRVIGNPQPNIQWKKDERWLQGDDSQFNLMDNGTLHIA 509

  Fly   503 DLQLSDSGTYTCTASGERGETSWAATLTVEKPGSTSLHRAADPSTYPAPPGTPKVLNVSRTSISL 567
            .:|..|.|.|:|.|....||.:|.:.|..::....|...|..||..|.||..|.|..|:..||:|
Mouse   510 SIQEMDMGFYSCVAKSSIGEATWNSWLRKQEDWGASPGPATGPSNPPGPPSQPIVTEVTANSITL 574

  Fly   568 RWAKSQEKPGAVGPIIGYTVEYFSPDLQTGWIVAAQRVGDTQVTISGLTPGTSYVFLVRAENTQG 632
            .| |...:.||..  ..|.:|.||......|...|..|.....|||||.|.|.|:|||||....|
Mouse   575 TW-KPNPQSGATA--TSYVIEAFSQAAGNTWRTVADGVQLETYTISGLQPNTIYLFLVRAVGAWG 636

  Fly   633 ISVPSGLSNTIKTIEADFD-AASANDLSAARTLLTGKSVELIDASAINASAVRLEWMLHVSADEK 696
            :|.||.:|..::| :||.. :..|.|....:..|...:|.:.:.:.:....:::.|.  |....:
Mouse   637 LSEPSPVSEPVQT-QADSSLSRPAEDPWKGQRGLAEVAVRMQEPTVLGPRTLQVSWT--VDGPVQ 698

  Fly   697 YVEGLRIHYKDASVPSAQYHSITVMDASAESFVVGNLKKYTKYEFFLTPFFETIEGQ-----PSN 756
            .|:|.|:.::.|.:....:..:.:.....:|.|:..|....:.:..:     .::||     .|.
Mouse   699 LVQGFRVSWRIAGLDQGSWTMLDLQSPHKQSTVLRGLPPGAQIQIKV-----QVQGQEGLGAESP 758

  Fly   757 SKTALTYEDVPSAPPDNIQI--GMYNQTAGWVRWTPPPSQHHNGNLYGYKIEVSAGNTMKVLANM 819
            ..|....|:.||.||..:.:  |....::..|.|.||.....||.:..|:| ...||..:...|.
Mouse   759 FVTRSIPEEAPSGPPQGVAVALGGDRNSSVTVSWEPPLPSQRNGVITEYQI-WCLGNESRFHLNR 822

  Fly   820 TLNATTTSVLLNNLTTGAVYSVRLNSFTKAGDGPYSKPISLFMDPTHHVHPPRAHPSGTHDGRHE 884
            :......||..:.|..|.:|...:.:.|.||.|..|.|:.:.:.     .||.|.|.
Mouse   823 SAAGWARSVTFSGLLPGQIYRALVAAATSAGVGVASAPVLVQLP-----FPPAAEPG-------- 874

  Fly   885 GQDLTYHNNGNIPPGDINPTTHKKTTDYLSGPWLMV---LVCIVLLVLVISAAISMVYFKRKHQM 946
                          .:::....::....|..|..:.   ..|..|| |...||:     .|:.:.
Mouse   875 --------------PEVSEGLAERLAKVLRKPAFLAGSSAACGALL-LGFCAAL-----YRRQKQ 919

  Fly   947 TKELGHLSVVSDNEITALNINSKESL-----------------WI-------------DHHRGW- 980
            .|||.|.: .|.....|::....|.|                 |:             ...||.
Mouse   920 RKELSHYT-ASFAYTPAVSFPHSEGLSGSSSRPPMGLGPAAYPWLADSWPHPPRSPSAQEPRGSC 983

  Fly   981 --RTADTDKDSGLSESKLLSHVNSSQSNYNNSDGGTDYAEVDT--RNLTTFYNCRKSPDNPTPYA 1041
              ...|.| |...:|:.:..::..:....|.|..|..|:.:|.  ..|.||:.......:..|..
Mouse   984 CPSNPDPD-DRYYNEAGISLYLAQTARGANASGEGPVYSTIDPVGEELQTFHGGFPQHSSGDPST 1047

  Fly  1042 TTMIIGTSSSETCTKTTSISADKDSGTHSPYSDAFAGQVPAVPVVKSNYLQYPVE--PINWSEFL 1104
            .:.......||           .|||       |..||        ...|..||:  .::|.|.|
Mouse  1048 WSQYAPPEWSE-----------GDSG-------ARGGQ--------GKLLGKPVQMPSLSWPEAL 1086

  Fly  1105 PPPPEHPPPSSTYGYAQGSPESSRKSSKSAGSGIS--TNQSILNASIHSSSSGGFSAWGVSPQYA 1167
            ||    ||||......:| ||...|.|........  ..:|.|   :.|||||.          .
Mouse  1087 PP----PPPSCELSCPEG-PEEELKGSSDLEEWCPPVPEKSHL---VGSSSSGA----------C 1133

  Fly  1168 VACPPENVYSNPLSAVAGGTQNRYQITPTNQHPPQLPAYF---------ATTGPGGAVPPNHLPF 1223
            :..|......:|.|:.  |.|:...:||:...|||.|...         ...||..       |.
Mouse  1134 MVAPAPRDTPSPTSSY--GQQSTATLTPSPPDPPQPPTDIPHLHQMPRRVPLGPSS-------PL 1189

  Fly  1224 ATQRHAASEYQ---AGLNAARCAQSRACNSCDALATPSPMQ------------------------ 1261
            :..:.|.|.:.   .||.|.......        |:|||:.
Mouse  1190 SVSQPALSSHDGRPVGLGAGPVLSYH--------ASPSPVPSTASSAPGRTRQVTGEMTPPLHGH 1246

  Fly  1262 ----------------------PPPPVPVPEGWYQPVHPNSHPMHPTSSNHQIYQCSSECSDHSR 1304
                                  ||||:|.||      ...|.|:.        .:.:...|...|
Mouse  1247 RARIRKKPKALPYRREHSPGDLPPPPLPPPE------EETSWPLG--------LRAAGSMSSLER 1297

  Fly  1305 SSQSHKRQLQLEEHGSS--AKQRGGH----------HRRRAPVVQPCMESENENMLAEYEQRQYT 1357
            .....:|.:|....|:.  ..|||.|          .....|..:|...|..          |.|
Mouse  1298 ERSGERRVVQAVPLGAQPLGAQRGPHPDAALLGCAAEEAWLPYGRPSFLSHG----------QGT 1352

  Fly  1358 SDC----CNSSREGDTCSCSEGS 1376
            |.|    .||||..::...|.||
Mouse  1353 STCSTAGSNSSRGSNSSRGSRGS 1375

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo1	NP_476899.1	Ig	56..151	CDD:299845	49/97 (51%)
		I-set	56..150	CDD:254352	49/96 (51%)
		I-set	157..251	CDD:254352	38/93 (41%)
		Ig2_Robo	159..251	CDD:143201	36/91 (40%)
		I-set	255..341	CDD:254352	36/85 (42%)
		Ig3_Robo	272..341	CDD:143202	30/68 (44%)
		IG_like	351..436	CDD:214653	38/87 (44%)
		Ig	362..444	CDD:299845	37/86 (43%)
		I-set	445..531	CDD:254352	34/86 (40%)
		IGc2	459..521	CDD:197706	21/62 (34%)
		FN3	549..637	CDD:238020	35/87 (40%)
		FN3	673..762	CDD:238020	13/93 (14%)
		fn3	770..855	CDD:278470	23/86 (27%)
Robo3	XP_006510170.1	Ig	64..162	CDD:386229	49/97 (51%)
		Ig	170..254	CDD:386229	36/91 (40%)
		Ig	275..343	CDD:386229	30/68 (44%)
		Ig4_Robo	361..450	CDD:143203	37/88 (42%)
		Ig	451..531	CDD:386229	33/80 (41%)
		FN3	564..649	CDD:238020	34/87 (39%)
		FN3	678..761	CDD:238020	12/89 (13%)
		FN3	771..864	CDD:238020	25/93 (27%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	104	1.000	Domainoid score	I6680
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	602	1.000	Inparanoid score	I935
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000772
OrthoInspector	1	1.000	-	-		mtm8796
orthoMCL	1	0.900	-	-		OOG6_101537
Panther	1	1.100	-	-	O	PTHR12231
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X493
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	9	8.960