DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo1 and Robo4

DIOPT Version :9

Sequence 1:	NP_476899.1	Gene:	robo1 / 37603	FlyBaseID:	FBgn0005631	Length:	1395	Species:	Drosophila melanogaster
Sequence 2:	NP_001292144.1	Gene:	Robo4 / 100911068	RGDID:	727947	Length:	1021	Species:	Rattus norvegicus

Alignment Length:	1442	Identity:	275/1442 - (19%)
Similarity:	434/1442 - (30%)	Gaps:	600/1442 - (41%)

- Green bases have known domain annotations that are detailed below.

  Fly    36 LLLVLVASNGLPAVRGQYQSPRIIEHPTDLVVKKNEPATLNCKVEGKPEPTIEWFKDGEPVSTNE 100
            |||.::....|.:      .|:|:.||.|.:::.:.||.::|:..|:|.|||.|..:|:|:|...
  Rat    28 LLLFIMGGKALDS------PPQILVHPQDQLLQGSGPAKMSCRASGQPLPTIRWLLNGQPLSMAT 86

  Fly   101 KKSHRVQFKDGALFFYRTMQGKKEQDG--------GEYWCVAKNRVGQAVSRHASLQIAVLRDDF 157
            ...|.:| .||.|..:|.....:.||.        |.|.|.|.||:|.||||.|.|.:|||::||
  Rat    87 PDLHYLQ-SDGTLLLHRPPTHGRPQDDQNILSAILGVYTCEASNRLGTAVSRGARLSVAVLQEDF 150

  Fly   158 RVEPKDTRVAKGETALLECGPPKGIPEPTLIWIKDGVPLDDLKAMSFGASSRVRIVDGGNLLISN 222
            |::|:||....||:.:||||||.|.|:|::.|.|||.||    .:..|.    |.|.|.:|:::.
  Rat   151 RIQPRDTVAVVGESLVLECGPPWGYPKPSVSWWKDGKPL----VLQPGK----RTVSGDSLMVAR 207

  Fly   223 VEPIDEGNYKCIAQNLVGTRESSYAKLIVQVKPYFMKEPKDQVMLYGQTATFHCSVGGDPPPKVL 287
            .|..|.|.|.|:|.|..|.|||..|::.:|..|    :.|:.:.|..
  Rat   208 AEKNDTGTYMCMATNNAGQRESRAARVSIQESP----DHKEHLELLA------------------ 250

  Fly   288 WKKEEGNIPVSRARILHDEKSLEISNITPTDEGTYVCEAHNNVGQISARASLIVHAPPNFTKRPS 352
                        .||       ::.|:|..:                          |...|.|
  Rat   251 ------------VRI-------QLENVTLLN--------------------------PEPVKGP- 269

  Fly   353 NKKVGLNGVVQLPCMASGNPPPSVFWTKEGVSTLMFPNSSHGRQHVAADGTLQITDVRQEDEGYY 417
                              .|.|:| |....||
  Rat   270 ------------------KPGPAV-WLSWKVS--------------------------------- 282

  Fly   418 VCSAFSVVDSSTVRVFLQVSSLDERPPPIIQIGPANQTLPKGSVATLPCRATGNPSPRIKWFHDG 482
                                            |||
  Rat   283 --------------------------------GPA------------------------------ 285

  Fly   483 HAVQAGNRYSIIQGSSLRVDDLQLSDSGTYTCTASGERGETSWAATLTVEKPGSTSLHRAADPST 547
                                    :.:.:||.....:|.
  Rat   286 ------------------------APAQSYTALFRAQRD-------------------------- 300

  Fly   548 YPAPPGTPKVLNVSRTSISLRWAKSQEKPGAVGPIIGYTVEYFSPDLQTGWIVAAQRVGDTQVTI 612
             |...|:|                                          |         |:|.:
  Rat   301 -PRDQGSP------------------------------------------W---------TEVLL 313

  Fly   613 SGLTPGTSYVFLVRAENTQGISVPSGLSNTIKTIEADFDAASANDLSAARTLLTGKSVELIDASA 677
            .||
  Rat   314 DGL-------------------------------------------------------------- 316

  Fly   678 INASAVRLEWMLHVSADEKYVEGLRIHYKDASVPSAQYHSITVMDASAESFVVGNLKKYTKYEFF 742
            :||.                                                :|.|:....|||.
  Rat   317 LNAK------------------------------------------------LGGLRWGQDYEFK 333

  Fly   743 LTPFFETIEGQPSNSKTALTYEDVPSAPPDNIQIGMYNQTAGWVRWTPPPSQHHNGNLYGYKIEV 807
            :.|......|..||.......|.||||||..:.:...|.:. :|.|.|||:::|||.:.||:: .
  Rat   334 VRPSSGRARGPDSNVLLLRLPEQVPSAPPQEVTLRPGNGSV-FVSWAPPPAENHNGFIRGYQV-W 396

  Fly   808 SAGNTMKVLANMTLNATTTSVLLNNLTTGAVYSVRLNSFTKAGDGPYSKPISLFM---------D 863
            |.||.....||.|:....|.:.:.....|: |.|::.:.|.||.|..|.|:.|.:         |
  Rat   397 SLGNASLPAANWTVVGEQTQLEIAARMPGS-YCVQVAAVTGAGAGEPSIPVCLLLEQAMEQSARD 460

  Fly   864 PTHHVHPPRAHPSGTHDGRHEGQDLTYHNNGNIPPGDINPTTHKKTTDYLSGPWLMVLVCIVLLV 928
            |:.||                                  ..|.::....|..|.::....::|.:
  Rat   461 PSKHV----------------------------------SWTLEQLRATLKRPEVIASGAVLLWL 491

  Fly   929 LVISAAISMVYFKRKHQMTKELGHLSVVSDNEITALNINSKESLWIDHHRGWRTADTDKDSGLSE 993
            |::..|: .:|.:||..:....|.....|::.|....::..:|.|:..  .||:....:|  ||.
  Rat   492 LLLGIAV-CIYRRRKAGVHLGPGLYRYTSEDAILKHRMDHSDSPWLAD--TWRSTSGSRD--LSS 551

  Fly   994 SKLLSHVNSSQSNYNNSDGGTDYAE-VDTRNLTTFYNCRKS-----PDNPTPYATTMIIGTSSSE 1052
            |..||           |..|.|..: :|.|.....::.|..     ||..|.|.:.:...|||..
  Rat   552 SSSLS-----------SRLGVDPRDPLDGRRSLISWDPRSPGVPLLPDTSTFYGSLIAEQTSSPP 605

  Fly  1053 TCTKTTSISA----DKDSGTHSPY--SDAFAG--------------------------QVPAVPV 1085
            ......:.:|    .|.:||.||:  ||:...                          |..:.|:
  Rat   606 VRPSPQTPAARRLPPKLTGTSSPWASSDSLCSRRGLCSPRMSLAPAEAWKAKKKQELHQANSSPL 670

  Fly  1086 VKSNYLQYPVEPINW------SEFL---PPPPEHPP---PSSTYGYAQGSPESSRKSSKSAGSGI 1138
            ::.:   :|:|...|      |:.|   |.|....|   |.:...:....|:..|.||:.|...:
  Rat   671 LRGS---HPMEIWAWELGSRASKNLSQSPGPNTCSPREAPGAVVAWRALGPQLHRNSSELAARPL 732

  Fly  1139 -STNQSILNASIHSSSSGGFSAWGVSPQYAVACPPENVYSNPLSAVAGGTQNRYQITPTNQHPPQ 1202
             .|..|:..|..|            .||.......:...|:||.|.        .::..|...|.
  Rat   733 PPTPLSLRGAPSH------------DPQSQCVEKLQAPSSDPLPAA--------PLSVLNSSRPS 777

  Fly  1203 LP--AYFATTGPGGAVPPNHLPFATQRHAASEYQAGL----NAARCAQSRACNSCDALATP-SPM 1260
            .|  ::.:...||.    ::|..::......|.|..:    ..|.|.:     ..|...|| :.:
  Rat   778 SPQASFLSVPSPGS----SNLSSSSLSSLEEEDQDSVLTPEEVALCLE-----LSDGEETPTNSV 833

  Fly  1261 QPPPPVPVPEGWYQPVHPNSHPMHPTSSNHQIYQCSSECSDHSRSSQSHKRQL------------ 1313
            .|.|..|.|...|      .:...||||.         .:|..|:......::
  Rat   834 SPMPRAPSPPATY------GYISIPTSSG---------LADMGRAGGGVGSEVGNLLCPPRLCPT 883

  Fly  1314 QLEEHGSSAKQRGGHHRRRAPVVQPCMESENE-NMLAEYEQRQYTSDCCNSSREGDTCSCSEGSC 1377
            .....||.|...|.......|..:..:.|.:: :.||:....:..:...:|.  |.:....|..|
  Rat   884 PTPSEGSLANGWGSASEDNVPSARASLVSSSDGSFLADAHFARALAVAVDSF--GFSLEPREADC 946

  Fly  1378 LYAEAGE-PAPR 1388
            ::.:|.. |:||
  Rat   947 VFTDASSPPSPR 958

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo1	NP_476899.1	Ig	56..151	CDD:299845	38/102 (37%)
		I-set	56..150	CDD:254352	38/101 (38%)
		I-set	157..251	CDD:254352	39/93 (42%)
		Ig2_Robo	159..251	CDD:143201	37/91 (41%)
		I-set	255..341	CDD:254352	7/85 (8%)
		Ig3_Robo	272..341	CDD:143202	4/68 (6%)
		IG_like	351..436	CDD:214653	7/84 (8%)
		Ig	362..444	CDD:299845	6/81 (7%)
		I-set	445..531	CDD:254352	6/85 (7%)
		IGc2	459..521	CDD:197706	2/61 (3%)
		FN3	549..637	CDD:238020	8/87 (9%)
		FN3	673..762	CDD:238020	11/88 (13%)
		fn3	770..855	CDD:278470	27/84 (32%)
Robo4	NP_001292144.1	Ig	41..144	CDD:299845	38/103 (37%)
		I-set	42..143	CDD:254352	38/101 (38%)
		I-set	150..231	CDD:254352	38/88 (43%)
		Ig	152..236	CDD:299845	37/91 (41%)
		fn3	275..346	CDD:278470	27/378 (7%)
		fn3	361..443	CDD:278470	27/84 (32%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	544..563		8/31 (26%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	600..634		10/33 (30%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	726..810		19/107 (18%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	81	1.000	Domainoid score	I8282
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.910