DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment robo1 and unc5db

DIOPT Version :9

Sequence 1:	NP_476899.1	Gene:	robo1 / 37603	FlyBaseID:	FBgn0005631	Length:	1395	Species:	Drosophila melanogaster
Sequence 2:	XP_001342230.2	Gene:	unc5db / 100002445	ZFINID:	ZDB-GENE-060531-162	Length:	941	Species:	Danio rerio

Alignment Length:	845	Identity:	177/845 - (20%)
Similarity:	299/845 - (35%)	Gaps:	232/845 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly    17 TTNNPSRSRSSRMWLLPAWLLLVLVA-------------SNGLPAVRGQYQSPRIIEHPTDLVVK 68
            |....:|.|:.|  .|....:|.|.|             ::.|.:|.|..  |..::.|.|..:.
Zfish     7 TEMGSTRKRARR--FLEIITVLALCAPHASGRGPVGDALADSLSSVGGTL--PHFLQEPEDAYII 67

  Fly    69 KNEPATLNCKVEGKPEPTIE-WFK-DGEPVSTNEKKSHR-------VQFKDGALFFYRTMQGKKE 124
            ||.|..|.|:.    .|.:: :|| :||.|..|:..|..       |:.::..:..  |.|..::
Zfish    68 KNNPIKLRCRA----TPALQIFFKCNGEWVHQNQHSSQEYMDQNTGVKIREVTINV--TRQQVED 126

  Fly   125 QDGGE-YW--CVAKNRVGQAVSRHASLQIAVLRDDFRVEPKDTRVAKGETALLECGPPKGIPEPT 186
            ..|.| ||  |||.:.:|.:.||.|:::||.||.:|..:|:...|......:|.|.||:|:|...
Zfish   127 FHGPEDYWCLCVAWSHLGTSKSRRATVRIAYLRKNFEQDPQGKEVPIKGMIVLHCRPPEGVPMAK 191

  Fly   187 LIWIKDGVPLDDLKAMSFGASSRVRIVDGGNLLISNVEPIDEGNYKCIAQNLVGTRESSYAKLIV 251
            :.|:|:...:..|:      ...:......||:|:.....|..||.|:|.|:|..|.|:.|.::|
Zfish   192 VEWLKNEELVSALE------DDNIDTRADHNLIINEARLSDSANYTCLATNIVAKRRSTTATVVV 250

  Fly   252 QVKPYFMKEPKDQVMLYGQTATFHCSV--------------------GG---------------- 280
            .|...:        .|:.:.::  |||                    ||
Zfish   251 FVNGGW--------SLWTEWSS--CSVNCGRGVQKRSRTCTNPAPLNGGAFCEGMSVQKIACNAV 305

  Fly   281 -----------------------------DPPPKVLWKKEE----------GNIPVSRARILHDE 306
                                         ||||:...|..|          |.:.....:::||.
Zfish   306 CPVDGVWEEWSEWTMCSAQCERQRWRECNDPPPRHRGKMCEGEGQSTENCTGGLCTQNGKLMHDV 370

  Fly   307 KSLEISNITPTDEGTYVCEAHNNVGQISARASLIVHAPPNFTKRPSNKKVGLNGVVQLPCMASGN 371
            |...:.|  ..|...|...|...:..:....::.::       |.|..:.|:: |:....:|.|.
Zfish   371 KPQSMEN--SMDVALYSGLAAGVIAVVVLIVAITLY-------RKSQSEYGVD-VIDSSALAGGF 425

  Fly   372 PPPSVFWTKEGVSTLMFPNSSHGRQHVAADGTLQITDVRQEDEGYYVCSAFSVVDSSTVRVFLQV 436
            ...:...|::|...|:  |||     :..|.|:          |....|.....||....:|
Zfish   426 QSFNFKTTRQGNPLLL--NSS-----MQPDITV----------GQTYSSPICFQDSIDKELF--- 470

  Fly   437 SSLDERPPPI-------IQIGPANQT--LPKGSVATLPCRATGNPSPRIKWFHDGHAVQAGNRYS 492
               |..|..:       :.:|.|::.  ..|....|||..           .|..:::..|.:.:
Zfish   471 ---DPLPDKVKVQSSFMVSLGVADRAEYHVKAHPQTLPAN-----------LHPNYSMVEGKKTA 521

  Fly   493 IIQGSSLRV-DDLQLSDSGTYTCTASGERGETSWAATLTVEKPGSTSL--HRA-ADPSTYPAPPG 553
            .:..:.|.. ..:||..|..:     |..|     ..|.:...|.:.|  |.| ||.:::.
Zfish   522 TMAPNGLHTPGKIQLKTSAVF-----GHLG-----GRLVIPNAGVSLLVPHGAIADDTSWE---- 572

  Fly   554 TPKVLNVSRTSISLRWAKSQEKPGAVGPIIGYTVEYFSPDLQTGWIVAAQRVGDTQVTISGLTPG 618
            ...::|...||     |:|:|:   ...::|..:.|..|.|.....||        :||
Zfish   573 MYMLINQGETS-----AQSEER---CEILLGPEITYGPPGLSLSCPVA--------MTI------ 615

  Fly   619 TSYVFLVRAENTQGISVPSGLSNTIKTIEADFDAASA----NDLSAARTLLTGKSVELIDASAIN 679
             ::...|.|||...........|..:.|.:..|.:::    .|..:...||.......:....|.
Zfish   616 -AHCAEVSAENWNIKLKRQTKDNKWEEIMSVDDESTSCYCLMDSHSCHLLLDQPGCYALVGEPIT 679

  Fly   680 ASAV-RLEWMLHVSADEKYVE-GLRIHYKDASVPSAQYHSITVMDASAESFVVGNLKKYTKYEFF 742
            .:|: ||...:..|.:...:: .||:|..| ..|.|....:|     ||:...|.|.:..|..||
Zfish   680 DAAIKRLRIAVFGSMEASSLDYSLRVHCVD-DTPHAFQGVVT-----AETCRGGQLLEEPKTLFF 738

Fly 743 742
Zfish 739 738

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
robo1	NP_476899.1	Ig	56..151	CDD:299845	30/106 (28%)
		I-set	56..150	CDD:254352	30/105 (29%)
		I-set	157..251	CDD:254352	25/93 (27%)
		Ig2_Robo	159..251	CDD:143201	24/91 (26%)
		I-set	255..341	CDD:254352	20/160 (13%)
		Ig3_Robo	272..341	CDD:143202	19/143 (13%)
		IG_like	351..436	CDD:214653	18/84 (21%)
		Ig	362..444	CDD:299845	16/81 (20%)
		I-set	445..531	CDD:254352	15/95 (16%)
		IGc2	459..521	CDD:197706	10/62 (16%)
		FN3	549..637	CDD:238020	18/87 (21%)
		FN3	673..762	CDD:238020	19/72 (26%)
		fn3	770..855	CDD:278470
unc5db	XP_001342230.2	I-set	162..250	CDD:254352	25/93 (27%)
		Ig	164..250	CDD:299845	24/91 (26%)
		TSP1	256..307	CDD:214559	6/60 (10%)
		TSP1	312..360	CDD:214559	7/47 (15%)
		ZU5	538..632	CDD:279170	28/130 (22%)
		DD	848..941	CDD:301326
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910