Protein Alignment ppk12 and Scnn1a

DIOPT Version :10

• Sequence 1: NP_611672.1
• Gene: ppk12 / 37566
• FlyBaseID: FBgn0034730
• Length: 569
• Species: Drosophila melanogaster
• Sequence 2: NP_035454.3
• Gene: Scnn1a / 20276
• MGIID: 101782
• Length: 699
• Species: Mus musculus
• Alignment Length: 610
• Identity: 124/610 - (20%)
• Similarity: 228/610 - (37%)
• Gaps: 144/610 - (23%)

  Fly     2 EPS-PSAGTERQGSSLFLLKSFVRSLRQFLNQTSLHGLKFVGDSGLSSWERSFFLGSFVTALIIT 65
            ||| |...||.:.:.:...:|:....:.|.|.|::||...:..|..:..:.:|:...::....:.
Mouse    59 EPSEPRQPTEEEEALIEFHRSYRELFQFFCNNTTIHGAIRLVCSKHNRMKTAFWAVLWLCTFGMM 123

  Fly    66 VHLISNIYVKWDSTPVIIGISPQATSILKVPFPAITICNMNQVQRSLVANYREGSN---ESALLK 127
            ....:.::.::.|.||.:.|:..:.   |:.|||:|:|.:|..:.:.:....|..:   |..|..
Mouse   124 YWQFALLFEEYFSYPVSLNINLNSD---KLVFPAVTVCTLNPYRYTEIKEDLEELDRITEQTLFD 185

  Fly   128 LLCESDSW-----------------------------ESSEADEEFSASNFV-TNNLKISE---- 158
            |...:.|:                             ..:.|....|||:.| .||.::..
Mouse   186 LYKYNSSYTRQAGGRRRSTRDLRGALPHPLQRLRTPPPPNPARSARSASSSVRDNNPQVDRKDWK 250

  Fly   159 -------------FVSNHSQSCERMLLFCRF------SAVERNCSQLFQQILTDEGLCCVFNFQP 204
                         |...:|...:.:..:.||      |.:......|.::.|......|.||..|
Mouse   251 IGFQLCNQNKSDCFYQTYSSGVDAVREWYRFHYINILSRLPDTSPALEEEALGSFIFTCRFNQAP 315

  Fly   205 ------PEYLYKPFAN----NNRNLTNSDGFESVMWDPESGYPEQLPPKFYPATASGTGITLGFT 259
                  .::.:..:.|    ||:|.:|       :|                 .:|..|:..|.:
Mouse   316 CNQANYSQFHHPMYGNCYTFNNKNNSN-------LW-----------------MSSMPGVNNGLS 356

  Fly   260 AVLDAEMGEYYCSSTNGPGFKVYFHNPIEVPKVKEAGLISAIGYETNYRIEMVRAEAVPAI---- 320
            ..|..|..::....:...|.:|..|...|...:.:.|.....|.||:  |.| |.||:.::
Mouse   357 LTLRTEQNDFIPLLSTVTGARVMVHGQDEPAFMDDGGFNVRPGVETS--ISM-RKEALDSLGGNY 418

  Fly   321 RSISRDGRQCLFKNEKELIFYRIYTRLNCENECLAAFLYDTCSCIPFDHPLIYSNASICSMGDTS 385
            ...:.:|.....||    ::...||:..|.:.|....:...|.|....:|. ......|.....|
Mouse   419 GDCTENGSDVPVKN----LYPSKYTQQVCIHSCFQENMIKKCGCAYIFYPK-PKGVEFCDYLKQS 478

  Fly   386 ----CVRRAQRASNRPG---WAKCRQQCLPSCFDLNY-LASGFS-----------FPLAS--NNF 429
                |..:.|.|.:...   ::|||:.|..:    || |::|:|           |.:.|  ||:
Mouse   479 SWGYCYYKLQAAFSLDSLGCFSKCRKPCSVT----NYKLSAGYSRWPSVKSQDWIFEMLSLQNNY 539

  Fly   430 QLANALVESFNKSYLSKNIAVINVYFRESVYYGNTKNAYVGLTEFLSNVGGVMGLFMGFSVIS-- 492
            .:.|.          ...:|.:|::|:|..|..|:::..|.:...|||:|....|:.|.||:|
Mouse   540 TINNK----------RNGVAKLNIFFKELNYKTNSESPSVTMVSLLSNLGSQWSLWFGSSVLSVV 594

  Fly   493 -LAEILYFLILKPLAELFVWKRSSH 516
             :||:::.|::..|..|....||.:
Mouse   595 EMAELIFDLLVITLIMLLHRFRSRY 619

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ppk12	NP_611672.1	ASC	29..499	CDD:459966	112/563 (20%)
Scnn1a	NP_035454.3	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..71		6/11 (55%)
		ENaC	79..676	CDD:273304	118/590 (20%)
		Gating release of inhibition by proteolysis (GRIP), protease-sensitive region that is responsible for the proteolytic activation of the channel. /evidence=ECO:0000305|PubMed:18650438	200..270		8/69 (12%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	211..244		7/32 (22%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	637..699
		PY motif, recruits WW domain-containing proteins and is thereby required for ubiquitination and inhibition of the channel by NEDD4 and NEDD4L. /evidence=ECO:0000250|UniProtKB:P37088	669..673