Protein Alignment Isha and SCAF4

DIOPT Version :10

• Sequence 1: NP_001097394.1
• Gene: Isha / 37411
• FlyBaseID: FBgn0034598
• Length: 1306
• Species: Drosophila melanogaster
• Sequence 2: NP_065757.1
• Gene: SCAF4 / 57466
• HGNCID: 19304
• Length: 1147
• Species: Homo sapiens
• Alignment Length: 1366
• Identity: 417/1366 - (30%)
• Similarity: 556/1366 - (40%)
• Gaps: 379/1366 - (27%)

  Fly     1 METVVAFNNELSGLYDSRPPISKAKMAAITKSAMRAIKLYKHVVQSVEKFILKCKPEYKVPGLYV 65
            |:.|.|||.||..|.|.:||||:|||..|||:|::||||||||||.|||||.|||||||||||||
Human     1 MDAVNAFNQELFSLMDMKPPISRAKMILITKAAIKAIKLYKHVVQIVEKFIKKCKPEYKVPGLYV 65

  Fly    66 IDSIVRQSRHQYGMDKDLFAPRFQRNLTETFANLFRCAPEDKSRIIRVLNLWQKNNVFKSEVIQP 130
            |||||||||||:|.|||:|.|||.:|:|.||..|:.|..||||:|:|||||||||.|||.|:|||
Human    66 IDSIVRQSRHQFGTDKDVFGPRFSKNITATFQYLYLCPSEDKSKIVRVLNLWQKNGVFKIEIIQP 130

  Fly   131 IFDLADPNHPIYHQMPPVGGSGGQGGGVGPGPSSSGALSLADISSGPNGLNSSGMEISMNSSGGD 195
            :.|:|...    ....||..:.....|..|.|        ..:||.|      ..:.:.||.
Human   131 LLDMAAGT----SNAAPVAENVTNNEGSPPPP--------VKVSSEP------PTQATPNSV--- 174

  Fly   196 DKMGGAMPDLSLGHEKFGG---------GSSSSKRHYSEQHYSKRQS------------VVSCSS 239
                .|:|.|. ..:.|..         |....:...:.|...|.||            .::...
Human   175 ----PAVPQLP-SSDAFAAVAQLFQTTQGQQLQQILQTFQQPPKPQSPALDNAVMAQVQAITAQL 234

  Fly   240 KTSKSHHHKREYV------KSSHDIDYQVREIIEEEDEGLQMLMADDHHCMGDDS-----PPTSS 293
            ||:.:...:::..      |::.|.....|...::|.|.::....:|...:...:     ||..:
Human   235 KTTPTQPSEQKAAFPPPEQKTAFDKKLLDRFDYDDEPEAVEESKKEDTTAVTTTAPAAAVPPAPT 299

  Fly   294 NLLDEKKIKQLLNNP-----------NVLRQLHTL-QNFQNLKPQEENQKHRYQDEALQQ---HF 343
            ..:.........:.|           .:.:..:|. ||....:|:.         ..:||   |.
Human   300 ATVPAAAAPAAASPPPPQAPFGFPGDGMQQPAYTQHQNMDQFQPRM---------MGIQQDPMHH 355

  Fly   344 QNVMKGNAGMPPGMGM---------------------------GMNMNDSMDLN-KDVEFISEQQ 380
            |..:..| |..||.|:                           ....|:.:... ...|...||.
Human   356 QVPLPPN-GQMPGFGLLPTPPFPPMAQPVIPPTPPVQQPFQASFQAQNEPLTQKPHQQEMEVEQP 419

  Fly   381 TIEVINLDGGDSRSPTPDRDRYKRSRRNSRSRTRSPRGRGAGGGTGNDRRRRGTRSRSRSRSPRS 445
            .|:.:.....|:|          :||  |||.:|||:.|               ||||.|||.||
Human   420 CIQEVKRHMSDNR----------KSR--SRSASRSPKRR---------------RSRSGSRSRRS 457

  Fly   446 SRRRG-SRDRDR---MDRSNRDKERDREHERERRKKGLPDIKKEHLSVCSTTLWVGHLSKLVYQE 506
            ..||. ||.|||   ..||...:.||||.|||||:||||.:|.|..||||||||||.|.|...|:
Human   458 RHRRSRSRSRDRRRHSPRSRSQERRDREKERERRQKGLPQVKPETASVCSTTLWVGQLDKRTTQQ 522

  Fly   507 ELSDTFGEYGDIVSIDQIVPRGCAFIVMNRRQDAHKAMQALK--NHKLQGRAITISWAAGKGVKS 569
            :::....|:|.|.||:.|.|||||:|||..||||::|:|.|.  |:|:..::|.|:||..||:|:
Human   523 DVASLLEEFGPIESINMIPPRGCAYIVMVHRQDAYRALQKLSRGNYKVNQKSIKIAWALNKGIKA 587

  Fly   570 KEWKDYWDLELGVTYIPWSKLSSDTDFDALEEGGMFDEDTMPIQMKQKINQAKNAAKEHKGASIA 634
             ::|.|||:|||||||||.|:..: :.::..||||.|.||:....|....:.:|...::.||..:
Human   588 -DYKQYWDVELGVTYIPWDKVKPE-ELESFCEGGMLDSDTLNPDWKGIPKKPENEVAQNGGAETS 650

  Fly   635 EAAGAPSVGVPPPNLIFGIDTTQPPPVGPVGPVGPP---PGPGAPP--PGLMGMVRGQFPMAPPM 694
            ..  .|...:|.|         .|.||.|: ||..|   |.|..||  ||            ||:
Human   651 HT--EPVSPIPKP---------LPVPVPPI-PVPAPITVPPPQVPPHQPG------------PPV 691

  Fly   695 GINMPPPIMMPPTNMPPPMMMPTTNMPPPMMMPPTMMPPGFPGIGGPPH--PMALPPGAPFP--P 755
            ...:.||...||..:|||...|....|||   ||..:.|||    .|.|  |..||||.|.|  |
Human   692 VGALQPPAFTPPLGIPPPGFGPGVPPPPP---PPPFLRPGF----NPMHLPPGFLPPGPPPPITP 749

  Fly   756 PGAVPPPIPSGGQISNSGNVSDDQMDIEMDLEDAPPLPP---------------QQQANFNP--S 803
            |.::|||......|.|| .::....|...||....|:|.               :...:..|  :
Human   750 PVSIPPPHTPPISIPNS-TIAGINEDTTKDLSIGNPIPTVVSGARGNAESGDSVKMYGSAVPPAA 813

  Fly   804 PSNVDLSPAAMANEMFQQRDRERDRDNRDRSRGPGNSRWGGRDDVVEAAERWRAENGGGGGGPGS 868
            |:|:...|......:.                                        |..|..||.
Human   814 PTNLPTPPVTQPVSLL----------------------------------------GTQGVAPGP 838

  Fly   869 GLGPNA-AFNEARARLNLNPIEH--GMPRPDFMDFDNRGGPGGPRGMGPRGNHNG--GGPGGDFF 928
            .:|..| :.....||..|.|::.  |||.|....|...    .||.|.|...|.|  .||||
Human   839 VIGLQAPSTGLLGARPGLIPLQRPPGMPPPHLQRFPLM----PPRPMPPHMMHRGPPPGPGG--- 896

  Fly   929 PPNMNHNRFNQPTSLMQMRIPPPASFNQRMGGPAGNGGNGVGPMFMRNQGGGPGGAGPSGGGPGG 993
                             ..:|||    ..|.||....|..|.|      ||.||..|| |.||||
Human   897 -----------------FAMPPP----HGMKGPFPPHGPFVRP------GGMPGLGGP-GPGPGG 933

  Fly   994 AGTGGG---------------------------GPGGRQQGPGFFNPRNPFNDNQRGRGGQSGGG 1031
            .....|                           .|..:|..|....|:...|||::    |...|
Human   934 PEDRDGRQQPPQQPQQQPQPQAPQQPQQQQQQQPPPSQQPPPTQQQPQQFRNDNRQ----QFNSG 994

  Fly  1032 VRGVGGGGGGGPGGRGRWSDDEDEGGNNFKRRGGPGGPGGNRFRG--ERGGEMMDDRRGNN---S 1091
                              .|.|..|..:|          |||...  ||.|...|||..:|   .
Human   995 ------------------RDQERFGRRSF----------GNRVENDRERYGNRNDDRDNSNRDRR 1031

  Fly  1092 RGGRGGP-------REDRERPGFGNRRGSRDDSNRHSISSTDEGNKAASETEPKPKNVETELGST 1149
            ..||..|       .|:|.|...|:|...||..:|.|....:|     :..:.||: |....|..
Human  1032 EWGRRSPDRDRHRDLEERNRRSSGHRDRERDSRDRESRREKEE-----ARGKEKPE-VTDRAGGN 1090

  Fly  1150 NTGAP--AAATRVDTEEDWDQELQDYEARMEAQKPGERASQSANSPPQEKLPAENENFAKPADVT 1212
            .|..|  :....|||..:.::.:                |::|...|.|:||||..:..:|...:
Human  1091 KTVEPPISQVGNVDTASELEKGV----------------SEAAVLKPSEELPAEATSSVEPEKDS 1139

  Fly  1213 G 1213
            |
Human  1140 G 1140

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Isha	NP_001097394.1	PHA03247	<1187..1306	CDD:223021	10/27 (37%)
		CID_SCAF8_like	6..135	CDD:340780	93/128 (73%)
		RRM_SCAF4_SCAF8	490..566	CDD:409674	38/77 (49%)
SCAF4	NP_065757.1	CID_SFRS15_SCAF4	5..135	CDD:340802	93/129 (72%)
		PRK12323	<133..281	CDD:481241	28/173 (16%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	145..179		9/54 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	235..254		2/18 (11%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	269..331		6/61 (10%)
		PAT1	<328..>412	CDD:401645	14/93 (15%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	424..502		43/104 (41%)
		RRM_SCAF4	504..584	CDD:409894	40/79 (51%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	629..661		6/33 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	879..1147		85/347 (24%)
		PRK12678	<984..>1053	CDD:237171	25/100 (25%)