Protein Alignment Isha and scaf4b

DIOPT Version :10

• Sequence 1: NP_001097394.1
• Gene: Isha / 37411
• FlyBaseID: FBgn0034598
• Length: 1306
• Species: Drosophila melanogaster
• Sequence 2: NP_001373157.1
• Gene: scaf4b / 552961
• ZFINID: ZDB-GENE-050506-42
• Length: 905
• Species: Danio rerio
• Alignment Length: 1069
• Identity: 330/1069 - (30%)
• Similarity: 465/1069 - (43%)
• Gaps: 318/1069 - (29%)

  Fly     1 METVVAFNNELSGLYDSRPPISKAKMAAITKSAMRAIKLYKHVVQSVEKFILKCKPEYKVPGLYV 65
            ||.|.|||.|:|.:.|..||||:|||.::||:.::||:|||||||.|||||.:|||:.|||||||
Zfish     3 MEAVNAFNGEMSSMMDMTPPISRAKMMSVTKAGIKAIRLYKHVVQIVEKFIKRCKPDLKVPGLYV 67

  Fly    66 IDSIVRQSRHQYGMDKDLFAPRFQRNLTETFANLFRCAPEDKSRIIRVLNLWQKNNVFKSEVIQP 130
            :|||:||||||:|.|||||.|||.:|.|.||.|||.|..:||.:|:|||||||||.||..|:|||
Zfish    68 VDSIIRQSRHQFGADKDLFGPRFLKNFTVTFQNLFECPEDDKGKILRVLNLWQKNEVFGLEIIQP 132

  Fly   131 IFDL----------------ADPNHPIYHQMP---PVGGSGGQGGGVGPGP--------SSSGAL 168
            :.|:                |.|..|:...:|   ||...       ||.|        ..:.||
Zfish   133 LVDMATAPVLPVLENGTTVTASPAIPVEVPVPVLVPVPAP-------GPAPVPVPVPILEPAPAL 190

  Fly   169 SLADISSGPNGLNSSGMEIS-----MNSSGGDDKMGGAMPDLSLGHEKFGG-GSSSSKRHYSEQH 227
            :.|.:::.|.....:.:.::     :..:||.:     :..|....::.|| |....|...:|:.
Zfish   191 APAVVTAAPPPTLQTPVPLAAVAQLLQGTGGVE-----LQQLLQTLQQAGGAGPPQPKAPPTEKK 250

  Fly   228 YSKRQSVVSCSSKTSKSHHHKREYVKSSHDIDYQVR-EIIEEEDEGLQMLMADDHHCMGDDSPPT 291
            .|..:|::.                    ..||... |.:||:.|..             .:.|.
Zfish   251 PSLAKSLLD--------------------RFDYDDEPEPVEEKPEPA-------------PAAPI 282

  Fly   292 SSNLLDEKKIKQLLNNPNVLRQLHTLQNFQN-------LKPQEENQKHRYQDEALQQHFQNVMKG 349
            :.||..|  ::|.|       |.|.|....|       :.||.:...........|...|..|..
Zfish   283 TINLPTE--LQQAL-------QAHLLSQIVNQTQMQAQMPPQVQMVPQVQIPPQAQMPPQIQMPP 338

  Fly   350 NAGMPPGMGM--GMNMNDSMDLNKDVEFISEQ-----QTIEV-----INLDGGDSRSPT------ 396
            .|.|||.:.|  .:.|...|.:....|..|::     ||.::     :..:..::.|.|
Zfish   339 QAQMPPQIQMPPQVQMQPQMQVPFQGEMASQEFPAMVQTQQIPENTQVYEESMETSSTTNQPQAV 403

  Fly   397 --PDRDRY--KRSRRNSRSRTRSPRGRGAGGGTGNDRRRRGTRSRSRSRSPRSS-RRRGSRDRDR 456
              .:|..:  :..||:.|:|:|||..:     :.:..|.|.:||.||||..||. .|..||.|:|
Zfish   404 AETERSAHDGREKRRSRRTRSRSPHRQ-----SSSSSRSRRSRSGSRSRRERSRYHRTRSRSRER 463

  Fly   457 MDRS-------NRDKERDREHERERRKKGLPDIKKEHLSVCSTTLWVGHLSKLVYQEELSDTFGE 514
            ..||       .||::::||.|:|||:|..|.:|||.||||:||||:|.|.|...:::::....|
Zfish   464 RGRSPRPRSEERRDRDKEREREKERRQKAEPSVKKETLSVCTTTLWIGQLDKKTQRQDITSLMEE 528

  Fly   515 YGDIVSIDQIVPRGCAFIVMNRRQDAHKAMQALK--NHKLQGRAITISWAAGKGVKSKEWKDYWD 577
            :|.|.||:.|.|||||::.|..||||..|:..|.  :.|:..:.|.|:||..||:|| |:|.:||
Zfish   529 FGQIESINMIPPRGCAYVAMVHRQDAQTALNKLSKTSVKVNQKTIKIAWAMNKGIKS-EYKKFWD 592

  Fly   578 LELGVTYIPWSKLSSDTDFDALEEGGMFDEDTMPIQMKQKINQAKNAAKEHKGASIAEAAGAPSV 642
            :|.|:|:|||.|:..: |..:|:|||:||.:|:..:.|..:.:....|.|....:..:.|..|.|
Zfish   593 VERGITFIPWGKVKPN-DIQSLQEGGLFDPETLKPEWKAALAKKSVPASEGPEGNQEDGAALPMV 656

  Fly   643 GVPPPNLIFGIDTTQPPPVGPVGPV--GPPPGPGAPPPGLMGMVRGQFPMAPPMGINMPPPIMMP 705
            ....|        .||.|| ||..|  ||||....|||||   :.|..|  ||.   :|||: ..
Zfish   657 SQSTP--------VQPVPV-PVMNVVSGPPPIMSLPPPGL---ITGASP--PPF---LPPPV-FS 703

  Fly   706 PTNMPP------PMMMPTTNMP-------------------PPMMMPPT---------------- 729
            ||.|||      |.::|:...|                   ||..:|.|
Zfish   704 PTQMPPVFRPIIPPVIPSAMRPVIPPVIPPAIPVASVNPAVPPASVPETAVEEASEEPLKSPITH 768

  Fly   730 --------MMPPGFPGIG--------GPPH-PMAL-PPGAP---------FPPPGAVPPPIPSGG 767
                    :.||  ||:|        |||| |:.| ||..|         .|||...|..:|.
Zfish   769 VDSMGPQALSPP--PGMGAGLMRPRLGPPHTPVRLRPPFMPAFPNMPRPVHPPPSVAPQMMPP-- 829

  Fly   768 QISNSGNVSDDQMDIEMDLEDAPPL--------PPQQQANFNPSPSNVDLSPAAMANEMFQQRDR 824
            ::||.            ...||||:        ||.|:  |.|              :.|.:..|
Zfish   830 RVSNP------------TFSDAPPVRFRMLRMRPPGQE--FPP--------------QRFPRAPR 866

  Fly   825 ERDRDNRDRSRGPGNSRWGGRDDVVEAAE-RWRAENGGGGGGPGSGLGPNAAFNEARARLNLNPI 888
                        |....|  |:...|.|. |:|:|
Zfish   867 ------------PEEPHW--REQGAERAPLRFRSE------------------------------ 887

  Fly   889 EHGMPRPDFMDFDNRGGPGGPRGMGPRGN 917
                  |.|:.|     .|.||...|.||
Zfish   888 ------PPFLRF-----RGAPRIRSPFGN 905

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Isha	NP_001097394.1	PHA03247	<1187..1306	CDD:223021
		CID_SCAF8_like	6..135	CDD:340780	84/144 (58%)
		RRM_SCAF4_SCAF8	490..566	CDD:409674	31/77 (40%)
scaf4b	NP_001373157.1	CID_SFRS15_SCAF4	7..137	CDD:340802	84/129 (65%)
		PHA03247	<186..345	CDD:223021	38/205 (19%)
		RRM_SF	502..582	CDD:473069	33/79 (42%)
		PHA03247	<708..902	CDD:223021	55/280 (20%)