DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Hmg-2 and Hmgb1

DIOPT Version :9

Sequence 1:	NP_611553.1	Gene:	Hmg-2 / 37407	FlyBaseID:	FBgn0026582	Length:	376	Species:	Drosophila melanogaster
Sequence 2:	NP_001300823.1	Gene:	Hmgb1 / 15289	MGIID:	96113	Length:	215	Species:	Mus musculus

Alignment Length:	207	Identity:	48/207 - (23%)
Similarity:	89/207 - (42%)	Gaps:	27/207 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 EPTSKTPDA-VPESPVSLPIASQ-----SMDTATIEDFDEDKPAKGKKKAKN---PKGRHSDSDI 58
            |...|.||| |..|..|...:.:     :.:....||..:...|:.:::.|.   |||
Mouse    26 EHKKKHPDASVNFSEFSKKCSERWKTMSAKEKGKFEDMAKADKARYEREMKTYIPPKG------- 83

  Fly    59 GSELKKLAQRRINVAGAPKMPLNGYVRFMNDRREELRREQPQRTALEHTRIIGEEWHQLPEERKL 123
              |.||    :.....|||.|.:.:..|.::.|.:::.|.|..:..:..:.:||.|:....:.|.
Mouse    84 --ETKK----KFKDPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQ 142

  Fly   124 PYIEAAAKDKAIYQEQLQMF-LKEHPEIVANELAKAKKATKLDGSPKEKTPKGESALGKAKKTKA 187
            ||.:.|||.|..|::.:..: .|..|:.....:.||:|:.|    .||:....|....:.::.:.
Mouse   143 PYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKK----KKEEEDDEEDEEDEEEEEEE 203

  Fly   188 KPVKRQSEDPDD 199
            :....:.:|.|:
Mouse   204 EDEDEEEDDDDE 215

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Hmg-2	NP_611553.1	HMGB-UBF_HMG-box	76..141	CDD:238686	18/64 (28%)
Hmgb1	NP_001300823.1	Sufficient for interaction with HAVCR2. /evidence=ECO:0000269\|PubMed:22842346	1..97		20/83 (24%)
		Heparin-binding. /evidence=ECO:0000250\|UniProtKB:P10103	1..10
		LPS binding (delipidated). /evidence=ECO:0000250\|UniProtKB:P09429	3..15
		HMG_box_2	6..78	CDD:401091	12/51 (24%)
		NLS 1. /evidence=ECO:0000250\|UniProtKB:P63159	27..43		6/15 (40%)
		Nuclear localization signal (NLS) 1. /evidence=ECO:0000250\|UniProtKB:P63159	27..43		6/15 (40%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	76..95		7/31 (23%)
		LPS binding (Lipid A). /evidence=ECO:0000250\|UniProtKB:P09429	80..96		7/28 (25%)
		Cytokine-stimulating activity. /evidence=ECO:0000250\|UniProtKB:P09429	89..108		5/18 (28%)
		HMG_box	95..162	CDD:395407	18/66 (27%)
		Binding to AGER/RAGE. /evidence=ECO:0000250\|UniProtKB:P63159	150..183		8/32 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	161..215		10/57 (18%)
		NLS 2. /evidence=ECO:0000250\|UniProtKB:P63159	178..184		3/9 (33%)
		Nuclear localization signal (NLS) 2. /evidence=ECO:0000250\|UniProtKB:P63159	178..184		3/9 (33%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5648
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3288
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.930